LOCUS       NM_008121               3180 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus gap junction protein, alpha 5 (Gja5), transcript
            variant 2, mRNA.
ACCESSION   NM_008121
VERSION     NM_008121.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Sedmera,D., Olejnickova,V., Sankova,B., Kolesova,H., Bartos,M.,
            Kvasilova,A., Phillips,L.C., Bamforth,S.D. and Phillips,H.M.
  TITLE     Morphological, electrophysiological, and molecular alterations in
            foetal noncompacted cardiomyopathy induced by disruption of ROCK
            signalling
  JOURNAL   Front Cell Dev Biol 12, 1471751 (2024)
   PUBMED   39435333
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Gomez-Del Arco,P., Isern,J., Jimenez-Carretero,D.,
            Lopez-Maderuelo,D., Pineiro-Sabaris,R., El Abdellaoui-Soussi,F.,
            Torroja,C., Vera-Pedrosa,M.L., Grima-Terren,M., Benguria,A.,
            Simon-Chica,A., Queiro-Palou,A., Dopazo,A., Sanchez-Cabo,F.,
            Jalife,J., de la Pompa,J.L., Filgueiras-Rama,D., Munoz-Canoves,P.
            and Redondo,J.M.
  TITLE     The G4 resolvase Dhx36 modulates cardiomyocyte differentiation and
            ventricular conduction system development
  JOURNAL   Nat Commun 15 (1), 8602 (2024)
   PUBMED   39366945
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3180)
  AUTHORS   Skelin,L., Racetin,A., Kelam,N., Ogorevc,M., Znaor,L.,
            Saraga-Babic,M., Filipovic,N., Katsuyama,Y., Pogorelic,Z. and
            Vukojevic,K.
  TITLE     Connexin Expression Is Altered in the Eye Development of Yotari
            Mice: A Preliminary Study
  JOURNAL   Biomolecules 14 (9), 1174 (2024)
   PUBMED   39334940
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3180)
  AUTHORS   Boulgakoff,L., Sturny,R., Olejnickova,V., Sedmera,D., Kelly,R.G.
            and Miquerol,L.
  TITLE     Participation of ventricular trabeculae in neonatal cardiac
            regeneration leads to ectopic recruitment of Purkinje-like cells
  JOURNAL   Nat Cardiovasc Res 3 (9), 1140-1157 (2024)
   PUBMED   39198628
  REMARK    Erratum:[Nat Cardiovasc Res. 2024 Sep;3(9):1178. doi:
            10.1038/s44161-024-00548-3.. PMID: 39261682]
REFERENCE   5  (bases 1 to 3180)
  AUTHORS   Yao,Z.J., Jiang,Y.P., Yuan,D., Hong,P., He,M.J., Li,F.X., Xu,S.Y.,
            Lin,H.B. and Zhang,H.F.
  TITLE     Decreased connexin 40 expression of the sinoatrial node mediates
            ischemic stroke-induced arrhythmia in mice
  JOURNAL   Exp Neurol 376, 114773 (2024)
   PUBMED   38599368
  REMARK    GeneRIF: Decreased connexin 40 expression of the sinoatrial node
            mediates ischemic stroke-induced arrhythmia in mice.
REFERENCE   6  (bases 1 to 3180)
  AUTHORS   Schwarz,H.J., Chang,Y.S., Hennemann,H., Dahl,E., Lalley,P.A. and
            Willecke,K.
  TITLE     Chromosomal assignments of mouse connexin genes, coding for gap
            junctional proteins, by somatic cell hybridization
  JOURNAL   Somat Cell Mol Genet 18 (4), 351-359 (1992)
   PUBMED   1332196
REFERENCE   7  (bases 1 to 3180)
  AUTHORS   Ludwig,T., Ruther,U., Metzger,R., Copeland,N.G., Jenkins,N.A.,
            Lobel,P. and Hoflack,B.
  TITLE     Gene and pseudogene of the mouse cation-dependent mannose
            6-phosphate receptor. Genomic organization, expression, and
            chromosomal localization
  JOURNAL   J Biol Chem 267 (17), 12211-12219 (1992)
   PUBMED   1376319
REFERENCE   8  (bases 1 to 3180)
  AUTHORS   Hennemann,H., Suchyna,T., Lichtenberg-Frate,H., Jungbluth,S.,
            Dahl,E., Schwarz,J., Nicholson,B.J. and Willecke,K.
  TITLE     Molecular cloning and functional expression of mouse connexin40, a
            second gap junction gene preferentially expressed in lung
  JOURNAL   J Cell Biol 117 (6), 1299-1310 (1992)
   PUBMED   1318884
REFERENCE   9  (bases 1 to 3180)
  AUTHORS   Haefliger,J.A., Bruzzone,R., Jenkins,N.A., Gilbert,D.J.,
            Copeland,N.G. and Paul,D.L.
  TITLE     Four novel members of the connexin family of gap junction proteins.
            Molecular cloning, expression, and chromosome mapping
  JOURNAL   J Biol Chem 267 (3), 2057-2064 (1992)
   PUBMED   1370487
REFERENCE   10 (bases 1 to 3180)
  AUTHORS   Willecke,K., Hennemann,H., Dahl,E., Jungbluth,S. and Heynkes,R.
  TITLE     The diversity of connexin genes encoding gap junctional proteins
  JOURNAL   Eur J Cell Biol 56 (1), 1-7 (1991)
   PUBMED   1666038
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB621080.1 and BC053054.1.
            
            On Oct 27, 2012 this sequence version replaced NM_008121.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC053054.1, AK137069.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                BB621080.1         3-16
            15-3180             BC053054.1         1-3166
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 42.04 cM"
     gene            1..3180
                     /gene="Gja5"
                     /gene_synonym="5730555N10Rik; Cnx40; Cx40; Cxni; Gja-5"
                     /note="gap junction protein, alpha 5"
                     /db_xref="GeneID:14613"
                     /db_xref="MGI:MGI:95716"
     exon            1..84
                     /gene="Gja5"
                     /gene_synonym="5730555N10Rik; Cnx40; Cx40; Cxni; Gja-5"
                     /inference="alignment:Splign:2.1.0"
     exon            85..3123
                     /gene="Gja5"
                     /gene_synonym="5730555N10Rik; Cnx40; Cx40; Cxni; Gja-5"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..1194
                     /gene="Gja5"
                     /gene_synonym="5730555N10Rik; Cnx40; Cx40; Cxni; Gja-5"
                     /note="connexin-40; gap junction membrane channel protein
                     alpha 5; connexin i"
                     /codon_start=1
                     /product="gap junction alpha-5 protein"
                     /protein_id="NP_032147.1"
                     /db_xref="CCDS:CCDS17652.1"
                     /db_xref="GeneID:14613"
                     /db_xref="MGI:MGI:95716"
                     /translation="MGDWSFLGEFLEEVHKHSTVIGKVWLTVLFIFRMLVLGTAAESS
                     WGDEQADFRCDTIQPGCQNVCYDQAFPISHIRYWVLQIIFVSTPSLVYMGHAMHTVRM
                     QEKQKLRDAEKAKEAHRTGAYEYPVAEKAELSCWKEVDGKIVLQGTLLNTYVCTILIR
                     TTMEVAFIVGQYLLYGIFLDTLHVCRRSPCPHPVNCYVSRPTEKNVFIVFMMAVAGLS
                     LFLSLAELYHLGWKKIRQRFGKSRQGVDKHQLPGPPTSLVQSLTPPPDFNQCLKNSSG
                     EKFFSDFSNNMGSRKNPDALATGEVPNQEQIPGEGFIHMHYSQKPEYASGASAGHRLP
                     QGYHSDKRRLSKASSKARSDDLSV"
     regulatory      3099..3104
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gja5"
                     /gene_synonym="5730555N10Rik; Cnx40; Cx40; Cxni; Gja-5"
                     /note="hexamer: AATAAA"
     polyA_site      3123
                     /gene="Gja5"
                     /gene_synonym="5730555N10Rik; Cnx40; Cx40; Cxni; Gja-5"
                     /note="major polyA site"
ORIGIN      
        1 aggctcaaga gcaaataaca gtgggcagtt gaacagcagc cagagcctga agaagccaac
       61 tccagggagg aggaaaggaa gcagaaggct cggcctcggt ctcctactct tggcaagatg
      121 ggtgactgga gcttcctggg ggagttcctg gaggaggtcc acaagcactc cacagtcatc
      181 ggcaaggtct ggctcactgt cctgttcatt ttccgcatgc tggtcctggg caccgctgct
      241 gagtcctcct ggggagatga gcaggccgac ttccggtgcg ataccattca gcctggttgc
      301 caaaatgtct gctatgacca agccttcccc atctcccaca ttcgttattg ggtactgcag
      361 atcatctttg tgtccacgcc ttctctagtg tacatgggcc atgccatgca cactgtgcgc
      421 atgcaggaaa agcagaaatt gcgggatgct gagaaagcta aagaggccca ccgcactggt
      481 gcctatgagt acccagtagc cgaaaaggcc gagctgtcct gctggaaaga agtagatggg
      541 aagattgtcc tccagggcac cctactcaac acctatgtct gcaccattct gatccgcacc
      601 accatggagg tggccttcat cgtaggccag tacctcctct atgggatctt cctggatacc
      661 ctgcatgtct gccgcaggag tccctgtccc cacccagtca actgttatgt ttcgaggccc
      721 acggagaaga atgtcttcat tgtctttatg atggctgtgg ctggactgtc tctgtttctc
      781 agcctggctg aactctacca cctgggctgg aagaagatcc gacagcgctt tggcaagtca
      841 cggcagggtg tggacaagca ccagctgcct ggccctccca ccagcctcgt ccagagcctc
      901 actcctcccc ctgacttcaa tcagtgccta aagaacagct ccggagagaa attcttcagc
      961 gacttcagta ataacatggg ctcccggaag aatccagacg ctctggccac tggggaagtg
     1021 ccaaaccagg agcagattcc aggggaaggc ttcatccaca tgcactatag ccagaagcca
     1081 gagtacgcca gtggagcctc tgcgggccac cgccttcctc agggctacca tagtgacaaa
     1141 cggcgcctta gtaaggccag cagcaaagca aggtcagatg acctgtcagt gtgatcctcc
     1201 ttttagggag ggccaggccc aggagggatc taaggagtgt gaggcaggaa agaaacagtc
     1261 tctctcgtga tggtatactc tcctcagcac tactttgctc tgtctgtcct gggtctgggt
     1321 ggcattcatt ggcattcatt gctcgttatc ctcaaaatta cagttctgag tcttctcaat
     1381 gactgggcta ctcctcaagt ctcattccct cctactcatc taccagtatt cacagtgaag
     1441 ccatagtgga ggcaggatca ggaaccgtgg caaaatctgg tctggggagg acaggttcaa
     1501 caccagcaac ataccagata gaccccccac ccaccccaag tcttctcttt ggtactatgc
     1561 ccatcctctt gttttgtttt gttttgtttt ttcttaagag cccaaggttg agagtattca
     1621 tcaagtaatc tgccctgtat cattcctttg aatcagttgt ttccatgtgc catacctcgg
     1681 agtgctggtg aggtaactgt gggctgccct tggctgcatc tccttcctcc cagtcttaaa
     1741 ggggtatcct tggtccttgg ccagcatcag atttacaagt gccttggtat gtacctttgg
     1801 caaatgtttc atgttgatga tgtctcagcc ttactgccag gctagacacc cagctcgtaa
     1861 gggtgccaag acctccagaa gtcactgtgc acacaagcat cgctggaatt gccaccagct
     1921 gtcatcctgc ggtcctttat agtttaacct aatgacagaa accatcctgg ccggctcacc
     1981 caccgttcct ctaaaatctt tgttaacagg aatgtccaaa gtcaccatcc tctcttggat
     2041 cctggccaga ttaccagcca ccaagttcag tggaatttcc ccaggattta ctgaaacaaa
     2101 gagagcattg tgcttctcgg gttcttctgg gtctggggat gggggtggag ggggaaattc
     2161 tgctgtgaag atgaaaaggt ggggggagga cactgaaaaa ctattttccc ttctgtgtat
     2221 gccttccact gtctgccagc ttttaatgcc gagaggtgtg gtgatagcaa ttgaggccca
     2281 agatctctct tagaggcatc aaggagacat ggaagcagaa agggtccagt tggtttggac
     2341 ccttatttag tgcagatatt cctatgtttt cttggtaccc ctaggtacaa caggggagga
     2401 ctgtagaaaa tccctgcctt cctcagggtc tatcctgggg tatctactga gtggatccat
     2461 cttatgcaga tggaagaagc tcagcaccag ataccgagat ttacaaacac aatactgtgt
     2521 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtttgtgtgt gcatgcccat ccatctctgt
     2581 gtgcctctgt gtgtgtgtgc tcgtgtgtgc atgcagatag aatggtgaag aagagccctg
     2641 aagaacgata aggaaacatg tagagaaact tgggtctttc tgcttccttc tcagttgtct
     2701 ggcaggaggg ctgagcttgc ttcttaatca gcctaaagat caggcatgag aagttcagga
     2761 gggtgaaggg tcagaggtca gtccctgtcc cagtgaccca tctctgggtc cctctgctgg
     2821 catttggttg ctactaatgg atagaaagaa attgactgag gatacctggg aaaggaagac
     2881 gggctgttcc agtcccagtg catttcatcc attcccgaca ctggcactaa gactctctgt
     2941 tctggctatg gagtgtgtgt ttgttttctg aaatgggatt tttttgaatt agaaaatctg
     3001 agaccagtcg ttgatggtga ggtttttttt gttttctgtt tttttttttt tttttttttc
     3061 agaagagaaa gaggaaatac tatgtttgac tggttttaaa taaaatctat ctctacatgc
     3121 caaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//
