LOCUS       NM_001374758            3744 bp    mRNA    linear   ROD 28-AUG-2024
DEFINITION  Mus musculus GIT ArfGAP 1 (Git1), transcript variant 2, mRNA.
ACCESSION   NM_001374758 XM_006532949
VERSION     NM_001374758.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Liu,H., Yi,J., Zhang,C., Li,Y., Wang,Q., Wang,S., Dai,S., Zheng,Z.,
            Jiang,T., Gao,P., Xue,A., Huang,Z., Kong,F., Wang,Y., He,B.,
            Guo,X., Li,Q., Chen,J., Yin,G. and Zhao,S.
  TITLE     Macrophage GIT1 promotes oligodendrocyte precursor cell
            differentiation and remyelination after spinal cord injury
  JOURNAL   Glia 72 (9), 1674-1692 (2024)
   PUBMED   38899731
  REMARK    GeneRIF: Macrophage GIT1 promotes oligodendrocyte precursor cell
            differentiation and remyelination after spinal cord injury.
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Xu,T., Gao,P., Huang,Y., Wu,M., Yi,J., Zhou,Z., Zhao,X., Jiang,T.,
            Liu,H., Qin,T., Yang,Z., Wang,X., Bao,T., Chen,J., Zhao,S. and
            Yin,G.
  TITLE     Git1-PGK1 interaction achieves self-protection against spinal cord
            ischemia-reperfusion injury by modulating Keap1/Nrf2 signaling
  JOURNAL   Redox Biol 62, 102682 (2023)
   PUBMED   36963288
  REMARK    GeneRIF: Git1-PGK1 interaction achieves self-protection against
            spinal cord ischemia-reperfusion injury by modulating Keap1/Nrf2
            signaling.
REFERENCE   3  (bases 1 to 3744)
  AUTHORS   Schlienger,S., Yam,P.T., Balekoglu,N., Ducuing,H., Michaud,J.F.,
            Makihara,S., Kramer,D.K., Chen,B., Fasano,A., Berardelli,A.,
            Hamdan,F.F., Rouleau,G.A., Srour,M. and Charron,F.
  TITLE     Genetics of mirror movements identifies a multifunctional complex
            required for Netrin-1 guidance and lateralization of motor control
  JOURNAL   Sci Adv 9 (19), eadd5501 (2023)
   PUBMED   37172092
REFERENCE   4  (bases 1 to 3744)
  AUTHORS   Fass,D.M., Lewis,M.C., Ahmad,R., Szucs,M.J., Zhang,Q.,
            Fleishman,M., Wang,D., Kim,M.J., Biag,J., Carr,S.A., Scolnick,E.M.,
            Premont,R.T. and Haggarty,S.J.
  TITLE     Brain-specific deletion of GIT1 impairs cognition and alters
            phosphorylation of synaptic protein networks implicated in
            schizophrenia susceptibility
  JOURNAL   Mol Psychiatry 27 (8), 3272-3285 (2022)
   PUBMED   35505090
  REMARK    GeneRIF: Brain-specific deletion of GIT1 impairs cognition and
            alters phosphorylation of synaptic protein networks implicated in
            schizophrenia susceptibility.
REFERENCE   5  (bases 1 to 3744)
  AUTHORS   Nagahori,K., Kuramasu,M., Kawata,S., Yakura,T., Li,Z., Hirai,S.,
            Qu,N. and Itoh,M.
  TITLE     GIT1 is an untolerized autoantigen involved in immunologic
            disturbance of spermatogenesis
  JOURNAL   Histochem Cell Biol 157 (3), 309-319 (2022)
   PUBMED   35066604
  REMARK    GeneRIF: GIT1 is an untolerized autoantigen involved in immunologic
            disturbance of spermatogenesis.
REFERENCE   6  (bases 1 to 3744)
  AUTHORS   Angrand,P.O., Segura,I., Volkel,P., Ghidelli,S., Terry,R.,
            Brajenovic,M., Vintersten,K., Klein,R., Superti-Furga,G.,
            Drewes,G., Kuster,B., Bouwmeester,T. and Acker-Palmer,A.
  TITLE     Transgenic mouse proteomics identifies new 14-3-3-associated
            proteins involved in cytoskeletal rearrangements and cell signaling
  JOURNAL   Mol Cell Proteomics 5 (12), 2211-2227 (2006)
   PUBMED   16959763
REFERENCE   7  (bases 1 to 3744)
  AUTHORS   Yin,G., Zheng,Q., Yan,C. and Berk,B.C.
  TITLE     GIT1 is a scaffold for ERK1/2 activation in focal adhesions
  JOURNAL   J Biol Chem 280 (30), 27705-27712 (2005)
   PUBMED   15923189
  REMARK    GeneRIF: GIT1 has a role as a scaffold for ERK1/2 activation in
            focal adhesions
REFERENCE   8  (bases 1 to 3744)
  AUTHORS   Zhang,H., Webb,D.J., Asmussen,H., Niu,S. and Horwitz,A.F.
  TITLE     A GIT1/PIX/Rac/PAK signaling module regulates spine morphogenesis
            and synapse formation through MLC
  JOURNAL   J Neurosci 25 (13), 3379-3388 (2005)
   PUBMED   15800193
REFERENCE   9  (bases 1 to 3744)
  AUTHORS   Yin,G., Haendeler,J., Yan,C. and Berk,B.C.
  TITLE     GIT1 functions as a scaffold for MEK1-extracellular
            signal-regulated kinase 1 and 2 activation by angiotensin II and
            epidermal growth factor
  JOURNAL   Mol Cell Biol 24 (2), 875-885 (2004)
   PUBMED   14701758
  REMARK    GeneRIF: GIT1 serves as a scaffold protein to facilitate
            c-Src-dependent activation of MEK1-ERK1/2 in response to both G
            protein-coupled receptors and tyrosine kinase-coupled receptors
REFERENCE   10 (bases 1 to 3744)
  AUTHORS   Bagrodia,S., Bailey,D., Lenard,Z., Hart,M., Guan,J.L.,
            Premont,R.T., Taylor,S.J. and Cerione,R.A.
  TITLE     A tyrosine-phosphorylated protein that binds to an important
            regulatory region on the cool family of p21-activated
            kinase-binding proteins
  JOURNAL   J Biol Chem 274 (32), 22393-22400 (1999)
   PUBMED   10428811
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL607072.15.
            
            On Oct 11, 2019 this sequence version replaced XM_006532949.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11927932.3370027.1,
                                           SRR11927937.4984101.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-347               AL607072.15        74905-75251
            348-481             AL607072.15        80582-80715
            482-594             AL607072.15        81134-81246
            595-700             AL607072.15        81356-81461
            701-918             AL607072.15        81965-82182
            919-1013            AL607072.15        82264-82358
            1014-1056           AL607072.15        82689-82731
            1057-1105           AL607072.15        84635-84683
            1106-1178           AL607072.15        84973-85045
            1179-1276           AL607072.15        85538-85635
            1277-1388           AL607072.15        85989-86100
            1389-1522           AL607072.15        86216-86349
            1523-1906           AL607072.15        86485-86868
            1907-1960           AL607072.15        86940-86993
            1961-2047           AL607072.15        87121-87207
            2048-2121           AL607072.15        87297-87370
            2122-2304           AL607072.15        87497-87679
            2305-2368           AL607072.15        87796-87859
            2369-3744           AL607072.15        88013-89388
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 46.61 cM"
     gene            1..3744
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /note="GIT ArfGAP 1"
                     /db_xref="GeneID:216963"
                     /db_xref="MGI:MGI:1927140"
     exon            1..347
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     CDS             296..2581
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /EC_number="3.6.5.2"
                     /note="isoform 2 is encoded by transcript variant 2; G
                     protein-coupled receptor kinase-associated ADP
                     ribosylation factor GTPase-activating protein; ARF
                     GTPase-activating protein GIT1; ARF GAP GIT1;
                     GRK-interacting protein 1; G protein-coupled receptor
                     kinase-interactor 1"
                     /codon_start=1
                     /product="ARF GTPase-activating protein GIT1 isoform 2"
                     /protein_id="NP_001361687.1"
                     /db_xref="CCDS:CCDS88196.1"
                     /db_xref="GeneID:216963"
                     /db_xref="MGI:MGI:1927140"
                     /translation="MSRKGPRAEVCADCSAPDPGWASISRGVLVCDECCSVHRSLGRH
                     ISIVKHLRHSAWPPTLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPI
                     KSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQAN
                     FFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNGRTPIDYARQAGHHELAER
                     LVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADSLDLSELAKAAKKKLQALSNRLF
                     EELAMDVYDEVDRRENDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLAR
                     FNAREFATLIIDILSEAKRRQQGKSLSSPTDNLELSARSQSELDDQHDYDSVASDEDT
                     DQEPLPSAGATRNNRARSMDSSDLSDGAVTLQEYLELKKALATSEAKVQQLMKVNSSL
                     SDELRRLQREIHKLQAENLQLRQPPGPVPPPSLPSERAEHTLMGPGGSTHRRDRQAFS
                     MYEPGSALKPFGGTPGDELATRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASSVP
                     FTPSSPLLSCSQEGSRHASKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELSKEDEL
                     HPELESLDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAV
                     TEMASLFPKRPALEPVRSSLRLLNASAYRLQSECRKTVPPEPGAPVDFQLLTQQVIQC
                     AYDIAKAAKQLVTITTREKKQ"
     exon            348..481
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     exon            482..594
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     exon            595..700
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     exon            701..918
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1013
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..1056
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1105
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1178
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1276
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1388
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     exon            1389..1522
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     exon            1523..1906
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     exon            1907..1960
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     exon            1961..2047
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     exon            2048..2121
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     exon            2122..2304
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     exon            2305..2368
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     exon            2369..3744
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3722..3727
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /note="hexamer: AATAAA"
     polyA_site      3744
                     /gene="Git1"
                     /gene_synonym="Cat-1; p95Cat"
                     /note="major polyA site"
ORIGIN      
        1 gcttcctgca ggagcggcct gggccgcgca gggggcgggg agagcggcga gcggcggcgg
       61 ggcggcgcgg ggcggcgcgg ggcggcgcgg ggcggagggc gcccgagggc gagcgggcgg
      121 gcgggcacgg cgggttccgg gccagccccg ggggcggacg gttggccgcg cgcgcggagc
      181 ctcgccgggg ccttgcggga gccgggccgg gccgcagggc cgccgccgcc gcccgggccc
      241 gccgcccgcg ccccccacgc tggcccagag ggctgcggcc gcgtcgccgc tgaggatgtc
      301 ccggaagggg ccgcgagcgg aggtgtgtgc ggactgcagc gctccggacc ctggctgggc
      361 atctatcagc agaggtgtgc tggtttgtga cgagtgctgc agtgtgcacc ggagcctggg
      421 acgacacatc tccattgtca agcacctgcg ccacagcgcc tggcctccta cgctgctcca
      481 gatggtgcac acgcttgcca gcaacggggc caactccatc tgggagcact ccctgctgga
      541 ccctgcgcaa gtgcaaagtg gccggcgcaa agccaacccc caagataaag tccaccccat
      601 caagtcagag ttcatcaggg caaagtacca gatgctggcg ttcgtgcaca agcttccctg
      661 ccgtgatgat gatggggtca ccgccaaaga cctcagcaag caactgcact caagtgtgcg
      721 aacaggcaac ttggaaacat gtctgcgcct gctttccttg ggtgcccagg ccaacttttt
      781 tcacccagaa aagggcacta cacctctaca tgtggctgcc aaggcagggc agacactgca
      841 ggctgagctg ctggtagtgt atggggctga ccccgggtct cctgatgtca atggccgcac
      901 acccatcgac tatgccaggc aggcggggca ccatgaactg gcagaaaggc tagttgagtg
      961 ccagtatgag ctcactgacc ggttggcctt ctatctctgt ggacgaaagc ctgatcacaa
     1021 gaatgggcat tacatcatcc cacagatggc tgacagcttg gatctgtctg aattggccaa
     1081 agctgccaag aagaagctgc aagcgctcag caaccggctt tttgaggaac ttgccatgga
     1141 tgtgtatgat gaggtggatc ggagagaaaa tgatgctgtg tggctagcta cccaaaatca
     1201 tagcaccctg gtgacggagc gcagtgctgt gcccttcctg ccagtcaacc ctgaatactc
     1261 agccactcgg aatcagggac gacagaaatt ggcccgtttc aatgcccgag agtttgccac
     1321 cttgatcatc gacattctca gtgaggccaa acggaggcag cagggcaaga gtctaagcag
     1381 ccccacagac aaccttgagc tgtctgcacg gagccagagc gagctggatg accagcacga
     1441 ctacgacagc gtggcttccg acgaagacac ggaccaggag cccttgccca gtgcaggcgc
     1501 cacgcggaac aaccgtgcca ggagcatgga ctcctcagat ctgtcagatg gggcagtgac
     1561 actgcaggag tacctggagc tgaagaaggc tctggctacc tccgaggcca aggtgcaaca
     1621 gctcatgaag gtcaacagca gcctgagtga cgagctgcgg cggctgcaaa gggagatcca
     1681 caaattgcag gcagagaacc tgcagcttcg gcagccaccc ggaccagtgc ctccaccctc
     1741 acttcccagt gaacgggcag aacacaccct catggggcct ggtggaagca cccaccgcag
     1801 ggaccgccag gccttctcta tgtatgagcc aggctctgca ttgaagccct ttggaggtac
     1861 acctggggac gagctcgcca cccggctcca gcctttccac agcacggagc tcgaagacga
     1921 tgccatctac tcagtgcatg tccctgccgg cctttatcgg atccggaagg gggtgtctgc
     1981 ctcttctgtg cccttcactc cctcctcccc actgctgtcg tgctcccaag aaggaagtcg
     2041 ccacgcgagc aagctctcac gccacggcag tggtgcggac agtgactatg agaacacaca
     2101 gagtggggac cctctgcttg ggcttgaagg gaagcgattc ctagagctga gcaaggagga
     2161 tgagctgcac cctgagctgg agagcttaga tggagacctc gaccccgggc tccccagcac
     2221 cgaagacgtc atcctaaaga cagagcaggt caccaagaac attcaggaac tattgcgggc
     2281 agcccaggag ttcaaacatg acagctttgt accctgttca gaaaagatac atttggctgt
     2341 gactgagatg gcttctctct tcccaaagag gccagccctg gagcctgtgc gcagttcact
     2401 gcggctgctc aacgccagcg cctaccggct tcagagcgag tgccggaaga cagtgcctcc
     2461 agagccgggc gcccctgtgg acttccagct gctgactcag caggtgatcc agtgcgccta
     2521 tgacatcgcc aaggctgcca agcagctggt caccatcacc acccgagaga agaaacagtg
     2581 accactttcc ctgcccttac ccacaccctg ggacctcact ggccatggga gctgggccac
     2641 tccagacact aacccacccc caacagagcc actggcacaa gtgcccttag tgccaccgtt
     2701 ttcctctggc atccaggtgc cctggtgccc tcccctcaag ccccaaggac agaaatgtga
     2761 ggtgacagga gcctctgtct cccacattcc ataccctccc tctgtacata gcatccctgc
     2821 cctgctgatg tgcaggagcc tcaaggaacc cctcccagct cttcacctgc cttccaggcg
     2881 ccctcagcaa agggtgtggg gcagggacac tccagatggg gcagctccgc ctactgcacc
     2941 ctatccccat gagccagttt agccctgggg ggccgagcag gggcaccccc tcctttgtac
     3001 attatctctg gatgtctctg tcctgtagcc agcagccccc ccttgctgct ctttcatgcc
     3061 aaacggcccc tgcctagagc acaggcccca acttgtgtgc cagggtcccc agcagcaaac
     3121 actggaaagt cttttcctct ccctacccct taattttaac tctgtggtaa ctgagtgtcc
     3181 ctgcgttcct gcaactgagg atgtgagcgg cagtgccgtt ccagagacct ggcccacctg
     3241 gagctctgag gagcagggga accagagtag tggaaccagc attggaagtc agcccctctc
     3301 cccccacctg ggacagggag actgtcaggg tcaccagctg tggtccagtt gctgccccta
     3361 cttgtatccc tgtcctataa acttaaaagg aaaaccactt ccctccatct ctccccgacc
     3421 cctatgggaa gtgtgctggc caggcagagg acagcacctg cgcctggggc tggctcccca
     3481 gggtccccaa ctcagctggt ggctgtggaa ctgagcccct ccccctagcc tctgcaaggc
     3541 cagcacccac atcactacct gcacctacag tgacccaccc ctgaaggcct ggggatctgg
     3601 ctgcagctgg gaaggccggc aaggcactga cagcacccac cagctcttct ccccaaccac
     3661 cttccctagc ctgtggtggt gggtagctgt cagaacgtgt gtcatgtaca tttgtatcaa
     3721 aaataaataa gtgaccatgt gcca
//
