LOCUS       NM_024240               1364 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus GINS complex subunit 4 (Gins4), mRNA.
ACCESSION   NM_024240 NM_177459
VERSION     NM_024240.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Kafer,G.R. and Cesare,A.J.
  TITLE     A Survey of Essential Genome Stability Genes Reveals That
            Replication Stress Mitigation Is Critical for Peri-Implantation
            Embryogenesis
  JOURNAL   Front Cell Dev Biol 8, 416 (2020)
   PUBMED   32548123
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1364)
  AUTHORS   Zhu,L., Zhao,W., Lu,J., Li,S., Zhou,K., Jiang,W., Duan,X., Fu,L.,
            Yu,B., Cai,K.Q., Gao,G.F., Liu,W. and Fang,M.
  TITLE     Influenza virus matrix protein M1 interacts with SLD5 to block host
            cell cycle
  JOURNAL   Cell Microbiol 21 (8), e13038 (2019)
   PUBMED   31050118
  REMARK    GeneRIF: Influenza virus matrix protein M1 interacts with SLD5 to
            block host cell cycle.
REFERENCE   4  (bases 1 to 1364)
  AUTHORS   Gong,Z.Y., Kidoya,H., Mohri,T., Han,Y. and Takakura,N.
  TITLE     DNA damage enhanced by the attenuation of SLD5 delays cell cycle
            restoration in normal cells but not in cancer cells
  JOURNAL   PLoS One 9 (10), e110483 (2014)
   PUBMED   25334017
  REMARK    GeneRIF: Attenuation of SLD5 expression results in marked DNA
            damage in both normal cells and cancer cells, suggesting that it
            protects against DNA damage.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1364)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 1364)
  AUTHORS   Mohri,T., Ueno,M., Nagahama,Y., Gong,Z.Y., Asano,M., Oshima,H.,
            Oshima,M., Fujio,Y. and Takakura,N.
  TITLE     Requirement of SLD5 for early embryogenesis
  JOURNAL   PLoS One 8 (11), e78961 (2013)
   PUBMED   24244394
  REMARK    GeneRIF: Requirement of SLD5 for early embryogenesis.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1364)
  AUTHORS   Kang,Y.H., Galal,W.C., Farina,A., Tappin,I. and Hurwitz,J.
  TITLE     Properties of the human Cdc45/Mcm2-7/GINS helicase complex and its
            action with DNA polymerase epsilon in rolling circle DNA synthesis
  JOURNAL   Proc Natl Acad Sci U S A 109 (16), 6042-6047 (2012)
   PUBMED   22474384
REFERENCE   8  (bases 1 to 1364)
  AUTHORS   Labib,K. and Gambus,A.
  TITLE     A key role for the GINS complex at DNA replication forks
  JOURNAL   Trends Cell Biol 17 (6), 271-278 (2007)
   PUBMED   17467990
  REMARK    Review article
REFERENCE   9  (bases 1 to 1364)
  AUTHORS   Kong,L., Ueno,M., Itoh,M., Yoshioka,K. and Takakura,N.
  TITLE     Identification and characterization of mouse PSF1-binding protein,
            SLD5
  JOURNAL   Biochem Biophys Res Commun 339 (4), 1204-1207 (2006)
   PUBMED   16338220
  REMARK    GeneRIF: These data suggest that PSF1 and SLD5 may cooperate in the
            proliferation of immature cell populations.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY309805.1, BG342391.1, AK076104.1 and BB750165.1.
            
            On Apr 28, 2009 this sequence version replaced NM_024240.5.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK076104.1, BC002156.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                BY309805.1         2-15
            15-35               BG342391.1         7-27
            36-1349             AK076104.1         28-1341
            1350-1364           BB750165.1         428-442
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 11.42 cM"
     gene            1..1364
                     /gene="Gins4"
                     /gene_synonym="2810037C03Rik; 4933405K01Rik; Sld5"
                     /note="GINS complex subunit 4"
                     /db_xref="GeneID:109145"
                     /db_xref="MGI:MGI:1923847"
     exon            1..199
                     /gene="Gins4"
                     /gene_synonym="2810037C03Rik; 4933405K01Rik; Sld5"
                     /inference="alignment:Splign:2.1.0"
     exon            200..319
                     /gene="Gins4"
                     /gene_synonym="2810037C03Rik; 4933405K01Rik; Sld5"
                     /inference="alignment:Splign:2.1.0"
     CDS             224..895
                     /gene="Gins4"
                     /gene_synonym="2810037C03Rik; 4933405K01Rik; Sld5"
                     /note="GINS complex subunit 4 (Sld5 homolog)"
                     /codon_start=1
                     /product="DNA replication complex GINS protein SLD5"
                     /protein_id="NP_077202.1"
                     /db_xref="CCDS:CCDS22190.1"
                     /db_xref="GeneID:109145"
                     /db_xref="MGI:MGI:1923847"
                     /translation="MTEVLDLHGQDSDGGSEEMVLTPAELIEKLEQAWMNEKFAPELL
                     ESKAEIVECVMEQLEHMEENLRRAKKGDLKVSIHRMEMERIRYVLSSYLRCRLMKIEK
                     FFPHILEKEKVRSEGEPSSLSPEEFVFAKEYMDHTETHFKNVALKHMPPNLQKVDLLR
                     AVPKPDLDSYVFLRVKERQENILVEPEADEQRDYVIDLEVGSQHLIRYKTIAPLVASG
                     AVQLI"
     exon            320..406
                     /gene="Gins4"
                     /gene_synonym="2810037C03Rik; 4933405K01Rik; Sld5"
                     /inference="alignment:Splign:2.1.0"
     exon            407..520
                     /gene="Gins4"
                     /gene_synonym="2810037C03Rik; 4933405K01Rik; Sld5"
                     /inference="alignment:Splign:2.1.0"
     exon            521..618
                     /gene="Gins4"
                     /gene_synonym="2810037C03Rik; 4933405K01Rik; Sld5"
                     /inference="alignment:Splign:2.1.0"
     exon            619..707
                     /gene="Gins4"
                     /gene_synonym="2810037C03Rik; 4933405K01Rik; Sld5"
                     /inference="alignment:Splign:2.1.0"
     exon            708..798
                     /gene="Gins4"
                     /gene_synonym="2810037C03Rik; 4933405K01Rik; Sld5"
                     /inference="alignment:Splign:2.1.0"
     exon            799..1364
                     /gene="Gins4"
                     /gene_synonym="2810037C03Rik; 4933405K01Rik; Sld5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1118..1123
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gins4"
                     /gene_synonym="2810037C03Rik; 4933405K01Rik; Sld5"
                     /note="hexamer: AATAAA"
     polyA_site      1148
                     /gene="Gins4"
                     /gene_synonym="2810037C03Rik; 4933405K01Rik; Sld5"
     regulatory      1353..1358
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gins4"
                     /gene_synonym="2810037C03Rik; 4933405K01Rik; Sld5"
                     /note="hexamer: AACAAA"
     polyA_site      1358
                     /gene="Gins4"
                     /gene_synonym="2810037C03Rik; 4933405K01Rik; Sld5"
                     /note="major polyA site"
ORIGIN      
        1 gtcgacgctg tgtttgggga aaggagcgcg ggagctgtac cgctgctggt cgggtctgtg
       61 ggccgcgggc tttccgcgcg atctccggcg cccaccgctc ggggagcctc agtccgtggt
      121 gtcccccgag cgtgccggct aggagagaag ggaagaggga tccctgagcc gtgtagccgc
      181 tttgagtgcc aactgcgagg tgtaggttcc cggttgagaa acgatgacgg aggttctgga
      241 tctccacgga caggactctg atgggggtag tgaggagatg gtcctaactc ctgcagagct
      301 cattgaaaag ctggagcagg cctggatgaa tgaaaagttt gcccctgagc tcctggaaag
      361 caaggctgag attgtcgaat gcgtcatgga acagcttgag cacatggaag aaaatctcag
      421 aagagccaag aagggggatc tgaaggtcag catccatcgc atggagatgg agaggatccg
      481 ctatgtcctc agcagctatt tgcggtgtcg actcatgaag atagagaagt ttttccctca
      541 catcctagaa aaggagaaag tgcgcagtga gggggagcct tccagcctgt ctccagagga
      601 gtttgtcttt gccaaagagt atatggacca cacggagacc cactttaaaa acgttgcctt
      661 aaagcacatg cctcccaacc tgcagaaggt ggacctcttg agggcagttc ccaaaccaga
      721 cctagattca tacgtgtttc tgcgagtgaa agaacgacaa gaaaacatac tagtagaacc
      781 agaagccgat gagcagagag actacgtgat tgacttggag gtgggctcac agcacttgat
      841 ccgatacaaa accatcgcac ctcttgttgc ttctggagca gttcagctaa tataaaacaa
      901 agcaagcctg aggacatgga gtggtcacag acaatattag aaacgccatc ttttgtgtct
      961 aatagtgaag tcatgttcca tacagttcgt cagcaatgtg tggaccactt ggacgtagaa
     1021 aggcgccgat gcagaattcc cttcgttgtc acagccttct cctaaattcc tgtttaacca
     1081 gcaccaacag atgggcccgt gctctgaata ctcagcaaat aaatcaaccg tcaggtgtgt
     1141 gccttttatt tttctactac ttactgagct caggaggggt ctcgaagagg cttccctgcc
     1201 ctgcccacga cggccagatg gactgggcat aacttgatga ttacagaact cctttagctt
     1261 tcctggtgga agatcataag cagttgtggt ctgcttctat acagcgttaa tatgtggcct
     1321 aatgatttgg aacctcaaat actaagctta aaaacaaatg tctc
//
