LOCUS       NM_027014               1100 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus GINS complex subunit 1 (Gins1), transcript variant 1,
            mRNA.
ACCESSION   NM_027014 XM_130416 XM_918384
VERSION     NM_027014.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Kang,Y.H., Galal,W.C., Farina,A., Tappin,I. and Hurwitz,J.
  TITLE     Properties of the human Cdc45/Mcm2-7/GINS helicase complex and its
            action with DNA polymerase epsilon in rolling circle DNA synthesis
  JOURNAL   Proc Natl Acad Sci U S A 109 (16), 6042-6047 (2012)
   PUBMED   22474384
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Nagahama,Y., Ueno,M., Miyamoto,S., Morii,E., Minami,T.,
            Mochizuki,N., Saya,H. and Takakura,N.
  TITLE     PSF1, a DNA replication factor expressed widely in stem and
            progenitor cells, drives tumorigenic and metastatic properties
  JOURNAL   Cancer Res 70 (3), 1215-1224 (2010)
   PUBMED   20103637
  REMARK    GeneRIF: Findings implicate PSF1 in tumorigenesis and offer initial
            evidence of its potential as a theranostic target.
REFERENCE   3  (bases 1 to 1100)
  AUTHORS   Ueno,M., Itoh,M., Sugihara,K., Asano,M. and Takakura,N.
  TITLE     Both alleles of PSF1 are required for maintenance of pool size of
            immature hematopoietic cells and acute bone marrow regeneration
  JOURNAL   Blood 113 (3), 555-562 (2009)
   PUBMED   18984863
REFERENCE   4  (bases 1 to 1100)
  AUTHORS   Labib,K. and Gambus,A.
  TITLE     A key role for the GINS complex at DNA replication forks
  JOURNAL   Trends Cell Biol 17 (6), 271-278 (2007)
   PUBMED   17467990
  REMARK    Review article
REFERENCE   5  (bases 1 to 1100)
  AUTHORS   Hayashi,R., Arauchi,T., Tategu,M., Goto,Y. and Yoshida,K.
  TITLE     A combined computational and experimental study on the
            structure-regulation relationships of putative mammalian DNA
            replication initiator GINS
  JOURNAL   Genomics Proteomics Bioinformatics 4 (3), 156-164 (2006)
   PUBMED   17127213
  REMARK    GeneRIF: expression of mammalian GINS is regulated by
            17beta-Estradiol-stimulated estrogen receptor alpha, and PSF3 acts
            as a gene responsive to transcription factor E2F1
REFERENCE   6  (bases 1 to 1100)
  AUTHORS   Kong,L., Ueno,M., Itoh,M., Yoshioka,K. and Takakura,N.
  TITLE     Identification and characterization of mouse PSF1-binding protein,
            SLD5
  JOURNAL   Biochem Biophys Res Commun 339 (4), 1204-1207 (2006)
   PUBMED   16338220
REFERENCE   7  (bases 1 to 1100)
  AUTHORS   Ueno,M., Itoh,M., Kong,L., Sugihara,K., Asano,M. and Takakura,N.
  TITLE     PSF1 is essential for early embryogenesis in mice
  JOURNAL   Mol Cell Biol 25 (23), 10528-10532 (2005)
   PUBMED   16287864
REFERENCE   8  (bases 1 to 1100)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   9  (bases 1 to 1100)
  AUTHORS   VanBuren,V., Piao,Y., Dudekula,D.B., Qian,Y., Carter,M.G.,
            Martin,P.R., Stagg,C.A., Bassey,U.C., Aiba,K., Hamatani,T.,
            Kargul,G.J., Luo,A.G., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Assembly, verification, and initial annotation of the NIA mouse
            7.4K cDNA clone set
  JOURNAL   Genome Res 12 (12), 1999-2003 (2002)
   PUBMED   12466305
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BQ553304.1, AK013116.1 and AW122649.1.
            
            On or before Jul 23, 2009 this sequence version replaced
            XM_918384.3, XM_130416.6.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: CF734948.1, AK013116.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 BQ553304.1         1-7
            8-1085              AK013116.1         1-1078
            1086-1100           AW122649.1         1-15                c
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 74.75 cM"
     gene            1..1100
                     /gene="Gins1"
                     /gene_synonym="2810418N01Rik; Gins4; mKIAA0186; PSF1"
                     /note="GINS complex subunit 1"
                     /db_xref="GeneID:69270"
                     /db_xref="MGI:MGI:1916520"
     exon            1..200
                     /gene="Gins1"
                     /gene_synonym="2810418N01Rik; Gins4; mKIAA0186; PSF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             126..716
                     /gene="Gins1"
                     /gene_synonym="2810418N01Rik; Gins4; mKIAA0186; PSF1"
                     /note="isoform 1 is encoded by transcript variant 1; DNA
                     replication complex GINS protein PSF1; GINS complex
                     subunit 4 (Sld5 homolog); GINS complex subunit 1 (Psf1
                     homolog)"
                     /codon_start=1
                     /product="DNA replication complex GINS protein PSF1
                     isoform 1"
                     /protein_id="NP_081290.1"
                     /db_xref="CCDS:CCDS50743.1"
                     /db_xref="GeneID:69270"
                     /db_xref="MGI:MGI:1916520"
                     /translation="MFCEKAMELVRELHRAPEGQLPAFNEDGLRQVLEEMKALYEQNQ
                     SDVNEAKSAGRGDLIPTVKFRHCALLRNRRCTIAYLYDRLLRIRALRWEYGSVLPNSL
                     RFHMSAEETEWFNHYKKSLATYMRSLGGDEGLDITQDVKPPKSLYIEVRCLKDYGEFE
                     VDDGTSVLLKKNSQHFLPRWKCEQLIRQGVLEHVLS"
     exon            201..265
                     /gene="Gins1"
                     /gene_synonym="2810418N01Rik; Gins4; mKIAA0186; PSF1"
                     /inference="alignment:Splign:2.1.0"
     exon            266..364
                     /gene="Gins1"
                     /gene_synonym="2810418N01Rik; Gins4; mKIAA0186; PSF1"
                     /inference="alignment:Splign:2.1.0"
     exon            365..455
                     /gene="Gins1"
                     /gene_synonym="2810418N01Rik; Gins4; mKIAA0186; PSF1"
                     /inference="alignment:Splign:2.1.0"
     exon            456..572
                     /gene="Gins1"
                     /gene_synonym="2810418N01Rik; Gins4; mKIAA0186; PSF1"
                     /inference="alignment:Splign:2.1.0"
     exon            573..647
                     /gene="Gins1"
                     /gene_synonym="2810418N01Rik; Gins4; mKIAA0186; PSF1"
                     /inference="alignment:Splign:2.1.0"
     exon            648..1087
                     /gene="Gins1"
                     /gene_synonym="2810418N01Rik; Gins4; mKIAA0186; PSF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1053..1058
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gins1"
                     /gene_synonym="2810418N01Rik; Gins4; mKIAA0186; PSF1"
                     /note="hexamer: AATAAA"
     polyA_site      1074
                     /gene="Gins1"
                     /gene_synonym="2810418N01Rik; Gins4; mKIAA0186; PSF1"
                     /note="major polyA site"
ORIGIN      
        1 aaagcgagga gctggttgct ggtgttgtgc gactctgaac aatagaagtg ccgcggcctg
       61 ggaactagcg accgtttggg gtgacttggc agttgacggg accacgggac ccgggcgcgt
      121 ccgtcatgtt ctgcgaaaaa gctatggagc ttgtccgcga gttacaccgc gcgccggaag
      181 ggcagctgcc ggcctttaat gaggacggac tcagacaagt tctggaggag atgaaagctt
      241 tgtatgaaca aaaccagtct gatgtgaatg aagccaagtc agctggacga ggggatctga
      301 taccaaccgt caaatttcgg cactgtgctt tgttaagaaa tagacgctgc acgatagcat
      361 acctgtatga ccggttgctt cggattagag cactcaggtg ggaatatggg agtgtcttgc
      421 caaatagttt acgattccac atgtctgctg aagaaacgga gtggttcaac cattataaaa
      481 agtctcttgc tacttacatg aggtcgctgg gaggagatga aggcttggac atcacacaag
      541 atgtgaagcc ccccaaaagc ctatatattg aagtgcggtg tttaaaagac tatggagaat
      601 ttgaagttga tgatggcact tcagtcctgc ttaaaaagaa tagtcagcac tttttgcctc
      661 ggtggaagtg tgagcagtta atcagacaag gagttctaga gcacgtgctg tcctgactgt
      721 gtcctgtggt gacagcagct ggatgtgaag ctaagccaac tcacagaagg aaacgtcctc
      781 actggacctc cccctccttg gttttaaatt gacagacatg cattaacata accaagatgg
      841 gctggggtcc agctcattgg tagagcacct gcttaggatt tgagaaaccc agagtttcat
      901 ccctagtacc tcaagaaaca agacaacaaa aatcaaagac aaaaccaaga actcttggct
      961 aggagcataa gctgccagct cttaagcctt tgttacaggt tatatctcta gttattccat
     1021 tcccacagta atattgtact cttataattg aaaataaaca gtctttggtt tgaatctgtt
     1081 ttcacaaaaa aaaaaaaaaa
//
