LOCUS       NM_001289652            4412 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus GID complex subunit 8 (Gid8), transcript variant 3,
            mRNA.
ACCESSION   NM_001289652 XM_006500731
VERSION     NM_001289652.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Francis,O., Baker,G.E., Race,P.R. and Adams,J.C.
  TITLE     Studies of recombinant TWA1 reveal constitutive dimerization
  JOURNAL   Biosci Rep 37 (1) (2017)
   PUBMED   27920276
  REMARK    GeneRIF: CD spectroscopy of recombinant TWA1 indicated a
            predominantly alpha-helical protein. Gel filtration chromatography,
            size-exclusion chromatography (SEC) with multi-angle light
            scattering (SEC-MALS) and native PAGE demonstrated a propensity of
            untagged TWA1 to form stable dimers and, to a lesser extent, higher
            order oligomers.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4412)
  AUTHORS   Umeda,M., Nishitani,H. and Nishimoto,T.
  TITLE     A novel nuclear protein, Twa1, and Muskelin comprise a complex with
            RanBPM
  JOURNAL   Gene 303, 47-54 (2003)
   PUBMED   12559565
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL732560.11, BY131338.1 and AK009106.1.
            
            On Apr 17, 2014 this sequence version replaced XM_006500731.1.
            
            Transcript Variant: This variant (3) differs in the 5' UTR,
            compared to variant 1. Variants 1, 2 and 3 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK009106.1, SRR9219382.72240.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 AL732560.11        72525-72533
            10-41               BY131338.1         1-32
            42-1546             AK009106.1         2-1506
            1547-4412           AL732560.11        81033-83898
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 103.26 cM"
     gene            1..4412
                     /gene="Gid8"
                     /gene_synonym="2310003C23Rik; 4833420G11Rik; Twa1"
                     /note="GID complex subunit 8"
                     /db_xref="GeneID:76425"
                     /db_xref="MGI:MGI:1923675"
     exon            1..125
                     /gene="Gid8"
                     /gene_synonym="2310003C23Rik; 4833420G11Rik; Twa1"
                     /inference="alignment:Splign:2.1.0"
     exon            126..255
                     /gene="Gid8"
                     /gene_synonym="2310003C23Rik; 4833420G11Rik; Twa1"
                     /inference="alignment:Splign:2.1.0"
     CDS             138..824
                     /gene="Gid8"
                     /gene_synonym="2310003C23Rik; 4833420G11Rik; Twa1"
                     /note="protein C20orf11 homolog; two hybrid-associated
                     protein 1 with RanBPM; GID complex subunit 8 homolog"
                     /codon_start=1
                     /product="glucose-induced degradation protein 8 homolog"
                     /protein_id="NP_001276581.1"
                     /db_xref="CCDS:CCDS17185.1"
                     /db_xref="GeneID:76425"
                     /db_xref="MGI:MGI:1923675"
                     /translation="MSYAEKPDEITKDEWMEKLNNLHVQRADMNRLIMNYLVTEGFKE
                     AAEKFRMESGIEPSVDLETLDERIKIREMILKGQIQEAIALINSLHPELLDTNRYLYF
                     HLQQQHLIELIRQRETEAALEFAQTQLAEQGEESRECLTEMERTLALLAFDSPEESPF
                     GDLLHMMQRQKVWSEVNQAVLDYENRESTPKLAKLLKLLLWAQNELDQKKVKYPKMTD
                     LSKGVIEEPK"
     exon            256..452
                     /gene="Gid8"
                     /gene_synonym="2310003C23Rik; 4833420G11Rik; Twa1"
                     /inference="alignment:Splign:2.1.0"
     exon            453..650
                     /gene="Gid8"
                     /gene_synonym="2310003C23Rik; 4833420G11Rik; Twa1"
                     /inference="alignment:Splign:2.1.0"
     exon            651..4412
                     /gene="Gid8"
                     /gene_synonym="2310003C23Rik; 4833420G11Rik; Twa1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4385..4390
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gid8"
                     /gene_synonym="2310003C23Rik; 4833420G11Rik; Twa1"
                     /note="hexamer: AGTAAA"
     polyA_site      4412
                     /gene="Gid8"
                     /gene_synonym="2310003C23Rik; 4833420G11Rik; Twa1"
                     /note="major polyA site"
ORIGIN      
        1 gcccccgccg ccgccgccgc ctccgcctcc tctatctccg cagtctctgc cgcccctccc
       61 cggccccggg agcggccgcg gagccgccac ccagacactg tcgcacggga ggcgggatcc
      121 gcacgaaata aatcataatg agttatgcag aaaagccgga tgaaataacc aaagatgaat
      181 ggatggaaaa gctcaataac ttgcatgttc aacgagcaga catgaaccga ctcatcatga
      241 actacctagt cacagagggc tttaaggaag cagcagagaa atttcggatg gagtctggga
      301 tcgaaccaag tgtggaccta gaaacacttg atgagcgaat caagattcgg gagatgattc
      361 tgaagggtca gatccaggag gccattgcct tgatcaacag cctccaccca gagctcctag
      421 acacaaaccg gtatctttac ttccaccttc agcaacaaca cttgattgag cttatccgtc
      481 agcgtgagac agaagcagca ttggagtttg cccagacaca actggcagag cagggtgagg
      541 aaagccgaga atgcctcaca gagatggagc gcacactggc cttgctggcc ttcgatagcc
      601 ctgaggagtc gccttttgga gacctccttc acatgatgca gaggcaaaag gtatggagtg
      661 aagttaacca ggctgttctg gattatgaga atcgagagtc cacacccaag ctggcaaaat
      721 tactgaaact gctactttgg gctcagaatg agctagacca gaagaaagta aaatatccca
      781 aaatgacaga cctcagcaaa ggtgtgattg aggagcccaa gtagagcctg tgcatggacc
      841 cagcactgtc aataggcaca ggactctccc tcctccggct gtgactggca gattgtcacc
      901 acagtagaga acctttcccc tgtacttttc tttggcccca ttgtttaaaa ggggaaaatg
      961 gcctttttaa accctgtcaa gcccatgatg gaagacacca cctctgccat gccctgtatc
     1021 ccgagagctg cacacactgc ttccagattg ctggttccag aagatgtaat ttgccactgt
     1081 gtgctgcacg gctagggagt gtcatctcag ctgaaggcct cacccaagac aggcagacag
     1141 acatgaacat tactgatgtg cttttccgac ctgctatgtg caggcaggca ggggttatct
     1201 tcctgggtct cacagacttt catggttttc cttacccctt cttttctttt tcttcctcat
     1261 tttgttcctt cataggaaaa aaatgtataa atttgtaaat cttaattcaa agataacttt
     1321 tgtgtgggta ttgagtttgg atcggttttt tgtcttggtg tgggctgtgt ggcttttggg
     1381 aggggatgtg tgtgagagag gtgaggttgt ggtggttttt attttttaaa atatattttt
     1441 ggtgctgtat gtgatgagag actcattcat agtggatcaa tgaataatct cttgtaggta
     1501 actttatttg aaaataaaga tttcctttta cttccagaat aaccacaatg gcttctaaaa
     1561 agttctcatc tcacacagcc cttgttttgg gtgtccttta ccttgcacag gggcagcacc
     1621 ttaactcaca gctgaaagac taattgccat gtgcctaggg aatgccctgc gtaataaata
     1681 gcaaagcagc ctggtgttga tgggatgact ggctggcatt acccagctgg atgttcatgc
     1741 tagccagatt gaacttaagt catttcttat ctcttccttc agtgtcagca gtgctgtgtg
     1801 tcctgcttct catcagccat tttttacctg ctcagtggaa ggcagaaaag acattacctg
     1861 tagggtatgg tatgcacatg taggtcggtg ctctgagtga tgagtgtata catgtattgt
     1921 gcctgacacc ggtgtgtttg atttttggca gtggtccagg cctgatggtg tcagtgctaa
     1981 aggctggggt gggggtgcac aaacctgcaa agccctcaga cagttatcca tggatgtgga
     2041 tggtgaagat gatgtgggta ctgtcctttg gtggaagttc ctgcctgtct gacactagag
     2101 ccaggagttc tggtactggc cattaagaaa cactgttctt agtaattact gaattgggat
     2161 tgtttccctt gttctttttt tttttttttt tttttttttt tttggttttc tgagacaggg
     2221 tttctctata tagccctggc tgtcctggaa ctcactctgt agactaggct ggactcgaac
     2281 tcagaaatct gcctgcctct gcctcctgag tgctaggatt aaagttgtgc gccaccacgc
     2341 ccggttgttt cgttgttctt gagcaatatt gactagctag gtcagactac atttattcaa
     2401 aagttttctg gcacctgagc tgctcagata acacagctga ccagctgtct ctatcttgtt
     2461 ccctacttca gtattgttca ttggtatctg ttttttaccc acaggattct gttcttgttg
     2521 ggatggaatg aagtgtcttc cagctctctg cttctactgc agagggcagg tactttggca
     2581 gtatttgcct taggatcaca ttagtgacag actcaaggat gaagttgttg ctgtcattat
     2641 gaccctgcac tgctcttctg gtctgtgagt aaggagtgag cagagctttc cttacctcct
     2701 ggacaggtgg ctttttaaca tcattgtgag atgtaacctc ccatctgggg taaggataag
     2761 aataaggtag aggagtttca ggagagacgt gcctttggtt aagaagacat gacaaataat
     2821 catgaagacc agagtccggc acccagaaaa gtcaggcatc ccccaaattc ctgtcacttc
     2881 agttccaagg aatctgatgt cctctttggc ctccacatac acataggcag gcaggcacaa
     2941 gaacacacac acacatacac acacgtcttt ttttaaggca gaggaaaagg aagttttaat
     3001 tttcaggtag ctaatagatt aaatgaaaca gaagttggga aggagtatcg aagattaagt
     3061 gtttgttttt aaagcaccat ataaagccag gcatggtggc tcctgcctgc aattacagca
     3121 tttacaaggc tgaggaaggg tttttccagt ccagccaagg ctatattata cataagatct
     3181 tttcttatat gaaaaagtac cagatagcta gcatatttgt atcttttgtt aatatatata
     3241 tatgattact tatatagcta atcactttga cgtgttctta aaattagagg gcatgagatg
     3301 tatagtaatg gaggttctct ctgtagggat tatcctcaaa tacacaggag gcttgttgca
     3361 tgcataaaat gttcagtggc tgtaagacaa ggagttgaca atttaccaac atttcagtca
     3421 gtagcttagc tgatgccaca gataccagtg actctggaga acgtcaaata gaactgcttg
     3481 cttatccatg gtctgttagt gcctagaaac cccctccaag gatttacatg tagacttttc
     3541 ctgttagagc agaccaagta gctttatttc tatttgcacc ttttgatgga aagtgccttg
     3601 aaataaacga gacaagaata ttctggtact cttctccaac gtcatacgta gagctgtgca
     3661 aggaaaaacc aagtttgaaa ttttctaaag cgtggtaatc tttgcacact aaagatgaga
     3721 atttgttttg tctcttgaat tatgatttgg gtttggcatt tgagaccccc tcctcgcccc
     3781 catagtgtat gtagttgaag actttgtata gtgactttga ggtggggcct gtagaactga
     3841 ggtgggcagt atgtcatctt tattttcttt tgtgctatta gttttgaaga ggagaaacca
     3901 ggagaatcct tccagaggga ttcttagatg ttccatgata atgacactga tgtgatcaag
     3961 agaaagaagt gggattccct tccaggttgt tgctctccag tctcagaaca atcaaaagac
     4021 caaattccaa atgtcaggtt aacagaacag tgtgtctaca cccctgcatt tcaacatgct
     4081 gaattgtgtg caggggacac catgcagggg tatagactgt tcaccagcct ttgcttactg
     4141 ctgctttgtt gacatgtctc gttctgacac catgcatatt ttttgtttca caaacattgc
     4201 ttttttcaga ttcttagtaa gccgccttgc cagcaggaga tgcagagtct gtataaacct
     4261 ctagctcgct ggagcactca tgtgtatatg taattgtaat tcttcattgg gggtatttgt
     4321 atccctgcca catatcatct gagtacaggt cgggtagctt ctgggtgcct gctgtgaatt
     4381 tgttagtaaa agttcttatg tttttcccag aa
//
