LOCUS       NM_019802               2926 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus gamma-glutamyl carboxylase (Ggcx), transcript variant
            1, mRNA.
ACCESSION   NM_019802
VERSION     NM_019802.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Lacombe,J., Guo,K., Bonneau,J., Faubert,D., Gioanni,F., Vivoli,A.,
            Muir,S.M., Hezzaz,S., Poitout,V. and Ferron,M.
  TITLE     Vitamin K-dependent carboxylation regulates Ca2+ flux and
            adaptation to metabolic stress in beta cells
  JOURNAL   Cell Rep 42 (5), 112500 (2023)
   PUBMED   37171959
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Shiba,S., Ikeda,K., Horie-Inoue,K., Azuma,K., Hasegawa,T.,
            Amizuka,N., Tanaka,T., Takeiwa,T., Shibata,Y., Koji,T. and Inoue,S.
  TITLE     Vitamin K-Dependent gamma-Glutamyl Carboxylase in Sertoli Cells Is
            Essential for Male Fertility in Mice
  JOURNAL   Mol Cell Biol 41 (4), e00404-20 (2021)
   PUBMED   33526452
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2926)
  AUTHORS   Lacombe,J., Rishavy,M.A., Berkner,K.L. and Ferron,M.
  TITLE     VKOR paralog VKORC1L1 supports vitamin K-dependent protein
            carboxylation in vivo
  JOURNAL   JCI Insight 3 (1), 96501 (2018)
   PUBMED   29321368
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2926)
  AUTHORS   Al Rifai,O., Chow,J., Lacombe,J., Julien,C., Faubert,D.,
            Susan-Resiga,D., Essalmani,R., Creemers,J.W., Seidah,N.G. and
            Ferron,M.
  TITLE     Proprotein convertase furin regulates osteocalcin and bone
            endocrine function
  JOURNAL   J Clin Invest 127 (11), 4104-4117 (2017)
   PUBMED   28972540
REFERENCE   5  (bases 1 to 2926)
  AUTHORS   Ferron,M., Lacombe,J., Germain,A., Oury,F. and Karsenty,G.
  TITLE     GGCX and VKORC1 inhibit osteocalcin endocrine functions
  JOURNAL   J Cell Biol 208 (6), 761-776 (2015)
   PUBMED   25753038
  REMARK    GeneRIF: OCN is gamma-carboxylated by the gamma-carboxylase (GGCX)
            on three glutamic acid residues, a cellular process requiring
            reduction of vitamin K by a second enzyme, VKORC1.
            Erratum:[J Cell Biol. 2019 Jun 3;218(6):2071. doi:
            10.1083/jcb.20140911104082019c.. PMID: 31040164]
REFERENCE   6  (bases 1 to 2926)
  AUTHORS   Li,Q. and Uitto,J.
  TITLE     The mineralization phenotype in Abcc6 (-/-) mice is affected by
            Ggcx gene deficiency and genetic background--a model for
            pseudoxanthoma elasticum
  JOURNAL   J Mol Med (Berl) 88 (2), 173-181 (2010)
   PUBMED   19784827
  REMARK    GeneRIF: These findings suggest a role for both the GGCX gene and
            the genetic background as well as dietary factors in modulating the
            phenotypic severity of Pseudoxanthoma elasticum caused by
            loss-of-function mutations in ABCC6.
REFERENCE   7  (bases 1 to 2926)
  AUTHORS   Zhu,A., Sun,H., Raymond,R.M. Jr., Furie,B.C., Furie,B.,
            Bronstein,M., Kaufman,R.J., Westrick,R. and Ginsburg,D.
  TITLE     Fatal hemorrhage in mice lacking gamma-glutamyl carboxylase
  JOURNAL   Blood 109 (12), 5270-5275 (2007)
   PUBMED   17327402
  REMARK    GeneRIF: Analysis of a Ggcx(+/-) intercross revealed a partial
            developmental block with only 50% of expected Ggcx(-/-) offspring
            surviving to term, with the latter animals dying uniformly at birth
            of massive intra-abdominal hemorrhage
REFERENCE   8  (bases 1 to 2926)
  AUTHORS   Czerwiec,E., Begley,G.S., Bronstein,M., Stenflo,J., Taylor,K.,
            Furie,B.C. and Furie,B.
  TITLE     Expression and characterization of recombinant vitamin K-dependent
            gamma-glutamyl carboxylase from an invertebrate, Conus textile
  JOURNAL   Eur J Biochem 269 (24), 6162-6172 (2002)
   PUBMED   12473112
REFERENCE   9  (bases 1 to 2926)
  AUTHORS   Pudota,B.N., Hommema,E.L., Hallgren,K.W., McNally,B.A., Lee,S. and
            Berkner,K.L.
  TITLE     Identification of sequences within the gamma-carboxylase that
            represent a novel contact site with vitamin K-dependent proteins
            and that are required for activity
  JOURNAL   J Biol Chem 276 (50), 46878-46886 (2001)
   PUBMED   11591726
REFERENCE   10 (bases 1 to 2926)
  AUTHORS   Begley,G.S., Furie,B.C., Czerwiec,E., Taylor,K.L., Furie,G.L.,
            Bronstein,L., Stenflo,J. and Furie,B.
  TITLE     A conserved motif within the vitamin K-dependent carboxylase gene
            is widely distributed across animal phyla
  JOURNAL   J Biol Chem 275 (46), 36245-36249 (2000)
   PUBMED   10893417
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC116115.11.
            
            On Aug 24, 2022 this sequence version replaced NM_019802.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK036577.1, AK135425.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-138               AC116115.11        178400-178537
            139-309             AC116115.11        178760-178930
            310-468             AC116115.11        181943-182101
            469-634             AC116115.11        182389-182554
            635-713             AC116115.11        186504-186582
            714-820             AC116115.11        188022-188128
            821-984             AC116115.11        189874-190037
            985-1250            AC116115.11        190449-190714
            1251-1382           AC116115.11        191130-191261
            1383-1534           AC116115.11        191986-192137
            1535-1704           AC116115.11        192546-192715
            1705-1835           AC116115.11        192885-193015
            1836-1983           AC116115.11        193221-193368
            1984-2179           AC116115.11        193532-193727
            2180-2926           AC116115.11        194055-194801
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 32.27 cM"
     gene            1..2926
                     /gene="Ggcx"
                     /note="gamma-glutamyl carboxylase"
                     /db_xref="GeneID:56316"
                     /db_xref="MGI:MGI:1927655"
     exon            1..138
                     /gene="Ggcx"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..2369
                     /gene="Ggcx"
                     /EC_number="4.1.1.90"
                     /note="isoform 1 is encoded by transcript variant 1;
                     vitamin K-dependent gamma-carboxylase; vitamin K gamma
                     glutamyl carboxylase; peptidyl-glutamate 4-carboxylase"
                     /codon_start=1
                     /product="vitamin K-dependent gamma-carboxylase isoform 1"
                     /protein_id="NP_062776.1"
                     /db_xref="CCDS:CCDS20242.1"
                     /db_xref="GeneID:56316"
                     /db_xref="MGI:MGI:1927655"
                     /translation="MAVHRGSALVAPASDKVQKNKSAQTSGLKQGSRMEKILGFEWTD
                     LSSWQSVVTLLNKPTDPANLAVFRFLFAFLMLLDIPQERGLSSLDRKYLDGLDVCRFP
                     LLDALRPLPLDWMYLVYTIMFLGALGMMLGLCYRLSCVLFLLPYWYVFLLDKTSWNNH
                     SYLYGLLAFQLTFMDANHYWSVDGLLNARKKNAHVPLWNYTVLRGQIFIVYFIAGVKK
                     LDADWVGGYSMEHLSRHWLFSPFKLVLSEELTSLLVVHWCGLLLDLSAGFLLFFDASR
                     PVGLFFVSYFHCMNSQLFSIGMFPYVMLASSPLFCSAEWPRKLVARCPKRLQELLPTK
                     AAPRPSASCVYKRSRGKAGPKPGLRHQLGAIFTLLYLLEQLFLPYSHFLTQGYNNWTN
                     GLYGYSWDMMVHSRSHQHVKITYRDGLTGELGYLNPGVFTQSRRWKDHADMLKQYATC
                     LSLLLPKYNVTEPQIYFDIWVSINDRFQQRLFDPRVDIVQAVWSPFQRTPWVQPLLMD
                     LSPWRTKLQDIKSSLDNHTEVVFIADFPGLHLENFVSEDLGNTSIQLLQGEVTVELVA
                     EQKNQTLQEGEKMQLPAGEYHKVYTVSSSPSCYMYVYVNTTEVALEQDLAYLQELKEK
                     VENGSETGPLPPELQPLLEGEVKGGPEPTPLVQTFLRRQRKLQEIERRRNSPFHERFL
                     RFVLRKLYVFRRSFLMTRISLRNLLLGRPSLEQLAQEVTYANLRPFEPVDESSASNTD
                     SSNHPSEPDSEHVHSEF"
     exon            139..309
                     /gene="Ggcx"
                     /inference="alignment:Splign:2.1.0"
     exon            310..468
                     /gene="Ggcx"
                     /inference="alignment:Splign:2.1.0"
     exon            469..634
                     /gene="Ggcx"
                     /inference="alignment:Splign:2.1.0"
     exon            635..713
                     /gene="Ggcx"
                     /inference="alignment:Splign:2.1.0"
     exon            714..820
                     /gene="Ggcx"
                     /inference="alignment:Splign:2.1.0"
     exon            821..984
                     /gene="Ggcx"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1250
                     /gene="Ggcx"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1382
                     /gene="Ggcx"
                     /inference="alignment:Splign:2.1.0"
     exon            1383..1534
                     /gene="Ggcx"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1704
                     /gene="Ggcx"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1835
                     /gene="Ggcx"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1983
                     /gene="Ggcx"
                     /inference="alignment:Splign:2.1.0"
     exon            1984..2179
                     /gene="Ggcx"
                     /inference="alignment:Splign:2.1.0"
     exon            2180..2926
                     /gene="Ggcx"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2536..2541
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ggcx"
                     /note="hexamer: ATTACA"
     polyA_site      2557
                     /gene="Ggcx"
     regulatory      2861..2866
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ggcx"
                     /note="hexamer: ATTAAA"
     polyA_site      2880
                     /gene="Ggcx"
     regulatory      2896..2901
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ggcx"
                     /note="hexamer: ATTAAA"
     polyA_site      2926
                     /gene="Ggcx"
                     /note="major polyA site"
ORIGIN      
        1 gtgtcgagga ggcggggcct aagacagcaa gtctaagtct ggaggttcca ctgggtccga
       61 cctggctgca gagaggctca cctgtccctg cagtcatggc tgtgcaccgc ggctccgcac
      121 tggttgctcc cgcctcagat aaagtacaga aaaacaagtc tgcacagaca tcaggactga
      181 aacagggcag ccgaatggag aaaattttag ggtttgaatg gacagattta tctagctggc
      241 agagtgtcgt gaccctgctt aacaaaccaa cggaccctgc aaacctggct gtctttcgtt
      301 ttctctttgc tttcttgatg ctgctggaca ttccccagga acgcggcctt agctccctgg
      361 accgaaaata cttggatggg ctggatgtgt gccgtttccc cttgctggat gccttgcgcc
      421 cactgccact ggactggatg tatcttgtct acaccatcat gtttctgggg gcactgggca
      481 tgatgctggg gctatgctac cggctaagct gtgtgttatt cctgctaccg tactggtacg
      541 tgtttctcct ggacaagact tcgtggaaca atcactccta tctgtatggt ttgttggcct
      601 ttcagttgac attcatggat gcaaaccact actggtctgt ggatggcttg ctgaatgccc
      661 gaaagaagaa tgctcacgtg cccctttgga actacacagt tctgcgtggc cagatcttca
      721 tcgtgtactt catcgcgggt gtgaagaagc tcgatgctga ctgggttggg ggctactcca
      781 tggagcacct gtcccggcac tggctcttca gtcccttcaa gctggtgttg tcggaggagc
      841 tgacaagcct gctggtagta cactggtgtg ggcttctcct tgacctctcg gctggcttcc
      901 tgctcttctt tgatgcctcc agacccgtcg gcctgttctt cgtgtcctac tttcactgca
      961 tgaactcgca gctcttcagc atcgggatgt ttccctatgt catgctggcc agcagccctc
     1021 tcttctgctc agctgaatgg cctcggaagt tggtagcccg atgcccgaaa aggctgcaag
     1081 agctgctgcc caccaaagcc gctcctcggc ctagtgcttc ctgtgtgtat aagaggtccc
     1141 ggggcaaagc tggcccgaag cccgggctgc gccaccagct gggagccatc ttcaccctgc
     1201 tctacctcct agagcagctc ttcctgccct attcccactt cctgacccag ggttacaata
     1261 actggacaaa tgggctgtat ggctattcct gggacatgat ggtgcactcc cgctcccacc
     1321 agcacgtaaa gatcacctac cgcgacggcc tcacgggcga gctgggctac cttaaccctg
     1381 gggtattcac acagagccgg cgatggaagg atcatgcaga catgctgaag caatatgcca
     1441 cttgcctgag cctcctgctt cccaagtaca atgtcactga gccccagatc tactttgata
     1501 tttgggtctc catcaatgac cgcttccagc agaggctttt tgaccctcgt gtggacatcg
     1561 tgcaggctgt ctggtccccc ttccagcgca caccttgggt gcagccactc ttgatggatt
     1621 tatctccctg gaggaccaag ttacaggata ttaagagcag tctggacaac cacaccgagg
     1681 tggtcttcat tgcagatttc cctgggcttc acttggagaa ttttgtgagt gaagacctgg
     1741 gcaacactag catccagctg ctgcagggag aagtcaccgt ggaattggtg gcagaacaga
     1801 aaaatcagac tcttcaagaa ggagagaaaa tgcagttgcc tgctggagag taccataaag
     1861 tctatactgt atcatctagt ccttcctgct acatgtacgt ctatgtcaac actacagagg
     1921 tcgcactgga gcaagacctg gcatatctgc aagaattaaa ggagaaggtg gagaacggaa
     1981 gtgaaacagg gcccctgcct ccagaacttc agcctctttt ggaaggggaa gtaaaagggg
     2041 gccctgagcc aacacctctg gtccaaactt ttctcagacg acagaggaag ctccaagaaa
     2101 ttgaacgcag gcgaaatagc cctttccatg agcgatttct ccgcttcgtg ctgcgaaagc
     2161 tctacgtctt tcgacgcagc ttcctgatga ctcgaatttc actccgaaac ctgctattag
     2221 gccgcccttc cctagagcaa ctagcccaag aggtgacata tgcaaacttg cgaccatttg
     2281 aaccagttga tgagtcaagt gcttcaaaca cagattcttc aaatcacccg tcagagccag
     2341 attctgagca tgttcactct gagttctgag ggatgtacag atgctctgtg cagatgtggg
     2401 ggcagcctgt tataggctta ttgtctacgc aaagaacata tttttggaga aaaatgatat
     2461 gggacaggct ttcacagtac agcccaggct ggcctcaaac tcatggttgg tccctctgct
     2521 tcagcctgtt ttgtaattac atagatcacc aaacctagtt gcttttccct ttacattttt
     2581 tccccttata agttctttaa aattatagct tacatttttt cttttttctt tttttttttt
     2641 ttgtattttt tctttttcaa gacagggtct ctctctgtgt agcactggct gtcctggaac
     2701 tcactctgta gtccaggctg gcctccaact cagaaattct cctgcctctg cctcccaagt
     2761 gctgggatta aaggtgtgtg ccaccacgcc ccactgggct tttagttttt atagacaaga
     2821 tttctccatg tagaccagac cagctctcct gagtgctgaa attaaaggca cgggacatca
     2881 ctacctggct ttcttattaa acttgtttta gtggtctcaa aattca
//
