LOCUS       NM_008115               3487 bp    mRNA    linear   ROD 21-MAY-2024
DEFINITION  Mus musculus glial cell line derived neurotrophic factor family
            receptor alpha 2 (Gfra2), transcript variant 1, mRNA.
ACCESSION   NM_008115
VERSION     NM_008115.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Morikawa,M., Yoshizaki,H., Yasui,Y., Nishida,S., Saikawa,Y.,
            Kohno,M. and Okajima,H.
  TITLE     Mesenchymal cells regulate enteric neural crest cell migration via
            RET-GFRA1b trans-signaling
  JOURNAL   Biochem Biophys Res Commun 710, 149861 (2024)
   PUBMED   38581949
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Vincent,E., Chatterjee,S., Cannon,G.H., Auer,D., Ross,H.,
            Chakravarti,A. and Goff,L.A.
  TITLE     Ret deficiency decreases neural crest progenitor proliferation and
            restricts fate potential during enteric nervous system development
  JOURNAL   Proc Natl Acad Sci U S A 120 (34), e2211986120 (2023)
   PUBMED   37585461
REFERENCE   3  (bases 1 to 3487)
  AUTHORS   Goodyer,W.R., Beyersdorf,B.M., Duan,L., van den Berg,N.S.,
            Mantri,S., Galdos,F.X., Puluca,N., Buikema,J.W., Lee,S., Salmi,D.,
            Robinson,E.R., Rogalla,S., Cogan,D.P., Khosla,C., Rosenthal,E.L.
            and Wu,S.M.
  TITLE     In vivo visualization and molecular targeting of the cardiac
            conduction system
  JOURNAL   J Clin Invest 132 (20), e156955 (2022)
   PUBMED   35951416
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3487)
  AUTHORS   Rios,A.S., De Vincenti,A.P., Casadei,M., Aquino,J.B.,
            Brumovsky,P.R., Paratcha,G. and Ledda,F.
  TITLE     Etv4 regulates nociception by controlling peptidergic sensory
            neuron development and peripheral tissue innervation
  JOURNAL   Development 149 (16) (2022)
   PUBMED   35904071
REFERENCE   5  (bases 1 to 3487)
  AUTHORS   Gadomski,S., Fielding,C., Garcia-Garcia,A., Korn,C., Kapeni,C.,
            Ashraf,S., Villadiego,J., Toro,R.D., Domingues,O., Skepper,J.N.,
            Michel,T., Zimmer,J., Sendtner,R., Dillon,S., Poole,K.E.S.,
            Holdsworth,G., Sendtner,M., Toledo-Aral,J.J., De Bari,C.,
            McCaskie,A.W., Robey,P.G. and Mendez-Ferrer,S.
  TITLE     A cholinergic neuroskeletal interface promotes bone formation
            during postnatal growth and exercise
  JOURNAL   Cell Stem Cell 29 (4), 528-544 (2022)
   PUBMED   35276096
REFERENCE   6  (bases 1 to 3487)
  AUTHORS   Wong,Y.W. and Too,H.P.
  TITLE     Identification of mammalian GFRalpha-2 splice isoforms
  JOURNAL   Neuroreport 9 (17), 3767-3773 (1998)
   PUBMED   9875703
REFERENCE   7  (bases 1 to 3487)
  AUTHORS   Jing,S., Yu,Y., Fang,M., Hu,Z., Holst,P.L., Boone,T., Delaney,J.,
            Schultz,H., Zhou,R. and Fox,G.M.
  TITLE     GFRalpha-2 and GFRalpha-3 are two new receptors for ligands of the
            GDNF family
  JOURNAL   J Biol Chem 272 (52), 33111-33117 (1997)
   PUBMED   9407096
REFERENCE   8  (bases 1 to 3487)
  AUTHORS   Widenfalk,J., Nosrat,C., Tomac,A., Westphal,H., Hoffer,B. and
            Olson,L.
  TITLE     Neurturin and glial cell line-derived neurotrophic factor
            receptor-beta (GDNFR-beta), novel proteins related to GDNF and
            GDNFR-alpha with specific cellular patterns of expression
            suggesting roles in the developing and adult nervous system and in
            peripheral organs
  JOURNAL   J Neurosci 17 (21), 8506-8519 (1997)
   PUBMED   9334423
REFERENCE   9  (bases 1 to 3487)
  AUTHORS   Suvanto,P., Wartiovaara,K., Lindahl,M., Arumae,U., Moshnyakov,M.,
            Horelli-Kuitunen,N., Airaksinen,M.S., Palotie,A., Sariola,H. and
            Saarma,M.
  TITLE     Cloning, mRNA distribution and chromosomal localisation of the gene
            for glial cell line-derived neurotrophic factor receptor beta, a
            homologue to GDNFR-alpha
  JOURNAL   Hum Mol Genet 6 (8), 1267-1273 (1997)
   PUBMED   9259272
REFERENCE   10 (bases 1 to 3487)
  AUTHORS   Baloh,R.H., Tansey,M.G., Golden,J.P., Creedon,D.J.,
            Heuckeroth,R.O., Keck,C.L., Zimonjic,D.B., Popescu,N.C.,
            Johnson,E.M. Jr. and Milbrandt,J.
  TITLE     TrnR2, a novel receptor that mediates neurturin and GDNF signaling
            through Ret
  JOURNAL   Neuron 18 (5), 793-802 (1997)
   PUBMED   9182803
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY257515.1, AK138512.1,
            AK149349.1, AI462772.1 and AC124202.3.
            
            On Oct 2, 2014 this sequence version replaced NM_008115.2.
            
            Summary: The protein encoded by this gene is part of the receptor
            complex that transduces glial cell-derived neurotrophic factor and
            neurturin signals by mediating autophosphorylation and activation
            of the RET receptor. Mice lacking this protein are viable and
            fertile but display growth retardation attributed to impaired
            salivary and pancreatic secretion and innervation deficits in the
            intestinal tract. In addition, knockout mice display neural defects
            including a failure to initiate outgrowth of dorsal ganglion root
            neurons, demonstrating a requirement in neuronal differentiation of
            these cells. Alternative splicing results in multiple transcript
            variants encoding different isoforms. [provided by RefSeq, Oct
            2014].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK149349.1, AK154579.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                BY257515.1         2-24
            24-2146             AK138512.1         2-2124
            2147-3485           AK149349.1         2129-3467
            3486-3486           AI462772.1         4-4                 c
            3487-3487           AC124202.3         24172-24172         c
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 36.79 cM"
     gene            1..3487
                     /gene="Gfra2"
                     /note="glial cell line derived neurotrophic factor family
                     receptor alpha 2"
                     /db_xref="GeneID:14586"
                     /db_xref="MGI:MGI:1195462"
     exon            1..788
                     /gene="Gfra2"
                     /inference="alignment:Splign:2.1.0"
     CDS             749..2143
                     /gene="Gfra2"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; GDNF family receptor alpha-2; GDNF receptor
                     beta; neurturin receptor alpha; TGF-beta-related
                     neurotrophic factor receptor 2; GFR alpha 2;
                     GDNFR-alpha-2; GDNFR-beta; NRTNR-alpha; NTNR-alpha"
                     /codon_start=1
                     /product="GDNF family receptor alpha-2 isoform 1
                     preproprotein"
                     /protein_id="NP_032141.2"
                     /db_xref="CCDS:CCDS27263.1"
                     /db_xref="GeneID:14586"
                     /db_xref="MGI:MGI:1195462"
                     /translation="MILANAFCLFFFLDETLRSLASPSSPQGSELHGWRPQVDCVRAN
                     ELCAAESNCSSRYRTLRQCLAGRDRNTMLANKECQAALEVLQESPLYDCRCKRGMKKE
                     LQCLQIYWSIHLGLTEGEEFYEASPYEPVTSRLSDIFRLASIFSGTGADPVVSAKSNH
                     CLDAAKACNLNDNCKKLRSSYISICNREISPTERCNRRKCHKALRQFFDRVPSEYTYR
                     MLFCSCQDQACAERRRQTILPSCSYEDKEKPNCLDLRSLCRTDHLCRSRLADFHANCR
                     ASYRTITSCPADNYQACLGSYAGMIGFDMTPNYVDSNPTGIVVSPWCNCRGSGNMEEE
                     CEKFLKDFTENPCLRNAIQAFGNGTDVNMSPKGPTFSATQAPRVEKTPSLPDDLSDST
                     SLGTSVITTCTSIQEQGLKANNSKELSMCFTELTTNISPGSKKVIKLYSGSCRARLST
                     ALTALPLLMVTLAQ"
     exon            789..1103
                     /gene="Gfra2"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1187
                     /gene="Gfra2"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1542
                     /gene="Gfra2"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1652
                     /gene="Gfra2"
                     /inference="alignment:Splign:2.1.0"
     exon            1653..1793
                     /gene="Gfra2"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..1966
                     /gene="Gfra2"
                     /inference="alignment:Splign:2.1.0"
     exon            1967..2020
                     /gene="Gfra2"
                     /inference="alignment:Splign:2.1.0"
     exon            2021..3487
                     /gene="Gfra2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3450..3455
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gfra2"
                     /note="hexamer: AATAAA"
     polyA_site      3470
                     /gene="Gfra2"
                     /note="major polyA site"
ORIGIN      
        1 gagggagagc ggagatcgag agcaaagagc gagagcgagg gagaggagag gagaggagag
       61 gagagggaga gaggaaggga ggaaagacac acgcaagcac aggcacaccg gcactggaat
      121 tccattagaa aaagtgagcc gaagcaaggg atagcgggag aagatttttt tttttttatt
      181 ttgaatcttt ttcttcgtct tggtgccaaa gaagcgactc tggtctcccg tcctagaagc
      241 tctactggat tgctcccatc cgtcggtgga tttctttcct atttgcattt attctgaccc
      301 ccctccctcg ctgctttctt ccagtccttc actttcagat cgcctcgccc ccacctctcc
      361 agtcccctcc tgggaagtgc aggggaattg gacccacggg gactcgcgcc ttcccggccg
      421 cgagagcgaa gggctgggct gacctcagga ccaggctgtt ggcttagaag gcagccagac
      481 acatagctac gtgtgtttga tttcagtggc aaggggggga cgtcgagagg cagcccaccg
      541 cccgcctccc acccctcccc ctccagccag cagcgagaat ccctggactc gggatcttca
      601 accggcggcc gcctggcggg atctccgcat tggatttggg ggtcgttatc attgcttggc
      661 tgttattatt accgttgtta ttttattttt attttttaaa cctaagggag aaagacacat
      721 acacacaaaa ctgtgggatt tatttaacat gatcttggca aacgccttct gcctcttctt
      781 ctttttagac gaaaccctcc gctctttggc cagcccttcc tctccgcagg gctctgagct
      841 ccacggctgg cgcccccaag tggactgtgt ccgggccaat gagctgtgtg cggctgaatc
      901 caactgcagc tccaggtacc gcacccttcg gcagtgcctg gcaggccggg atcgcaatac
      961 catgctggcc aataaggagt gccaggcggc cctggaggtc ttgcaggaaa gcccattgta
     1021 tgactgccgc tgcaagcggg gcatgaagaa ggagctgcag tgtctgcaga tctattggag
     1081 catccatctg gggctgacgg agggtgagga gttctacgaa gcttcgccct atgagcctgt
     1141 gacctcccgc ctctcggaca tcttcaggct cgcttcaatc ttctcaggga caggggcaga
     1201 cccggtggtc agtgccaaga gcaaccactg cctggatgcc gccaaggcct gcaacctgaa
     1261 cgacaactgc aagaagctcc gctcctccta catctccatc tgcaaccgcg agatctctcc
     1321 caccgaacgc tgcaaccgcc gcaagtgcca caaggccctg cgccagttct tcgaccgtgt
     1381 gcccagcgag tatacctacc gcatgctctt ctgctcctgt caggaccagg catgcgccga
     1441 gcgtcgccgg caaaccatcc tgcccagctg ttcctatgag gacaaagaga agcccaattg
     1501 cttggacctg cgcagcctgt gtcgtacaga ccacttgtgc cggtcccgcc tggcagactt
     1561 ccacgccaac tgtcgagcct cctaccggac aatcaccagc tgccctgcgg acaactacca
     1621 ggcatgtctg ggctcctatg ctggcatgat tgggtttgat atgacaccga actatgtgga
     1681 ctccaacccc acgggcatcg tggtgtctcc ctggtgcaat tgtcgtggca gtgggaacat
     1741 ggaagaagag tgtgagaagt tcctcaagga cttcacagaa aacccatgcc tccggaatgc
     1801 cattcaagcc tttggcaatg gcacagatgt gaacatgtct cccaaaggcc ccacattttc
     1861 agctacccag gcccctcggg tagagaaaac tccttcactg ccagatgacc tcagtgatag
     1921 caccagcctg gggaccagtg tcatcaccac ctgcacatct atccaggagc aagggctgaa
     1981 ggccaacaac tccaaagagt taagcatgtg tttcacagag ctcacgacaa atatcagccc
     2041 agggagtaaa aaggtgatca aactttactc aggctcctgc agagccagac tgtcgactgc
     2101 cttgactgcc ctcccactcc tgatggtgac cttggcccag taggcctttg gaacccagca
     2161 cgaaagttct tcaagcaacc cagatacgaa ctcccgcctg acaaaatgga aacatgcaca
     2221 cacacacaca cacacacaca cacacacaca cacacacaca cacagacaca cacacacaga
     2281 catattctct ctctctctct ctctctctct ctctctctct ctctctctct ctctcacaca
     2341 cacacacaca cacactcccc ttgcaaaaac actttttttc cctacattgc ctctgaacct
     2401 ttctcctccc aaatttcttc tctggagaag tttttctaaa ccaaacagac aagcaggcgg
     2461 gcagtcagaa gcctgcccag aggtccccta caagggacat ccagcaccga gggctcaagg
     2521 ctcttgagag acttttttct cttcactggt gttttctctc tggaccagat gagaccctgc
     2581 tgtggaagga acgttgctgt gcctggtgtg gactggggaa aggacaattg cggctgccca
     2641 ctctggggac ctgcccaagg gttcgcagag agtttcagtc agcaaggagc agggctagcc
     2701 acaaggacct tgtcacctct tcctcttggc ttcaaagatg gaaacggttt gctgccatcc
     2761 ctatccctta tggggcctag tggatctgag tctggggtag ggacccccag ggttgctcca
     2821 ggccgaggtg cctgcagcat ggtggggttg ggagctgtta caggacgaag cttctttggg
     2881 gaacaagcaa gccttggttg ggtaccagct ccaagatgct ctttcctcct tcatgccagg
     2941 aatcttgaag tcaaaaagag atgatcctct gttggctttt tttgtttgtt tgtttttgaa
     3001 tttttttgtg ggtccatttg gcgggtctct cttggggaga agggctgttg agcggggctg
     3061 gggacaccct agctggacgt gtgtacagaa cactctggtg ggctcccaag cttgcccctt
     3121 ctctcttctc cttgtttctg ctttctctct cctcatttct gagacattca tgcactgtct
     3181 gtacatactg gatctctttt ctcaacatat gtgtatatcc atatacatat atcctgtgat
     3241 ttttctttca tttttttaaa aaaaagaaac aaaactatgg aaataatacc ctacagatga
     3301 gcgaaaatgt attattgtaa agtttatttt ttttaataat gttgtctatg atgggaagaa
     3361 aggtaccagg acccctgagc tctggtccag ttgggctgat ggggctgtgg ccggggactc
     3421 ccgattgcat tcactcttaa ccaagctcca ataaacgtac taggaagcga gttgccccgt
     3481 gcttcac
//
