LOCUS       NM_013528               6248 bp    mRNA    linear   ROD 04-DEC-2024
DEFINITION  Mus musculus glutamine fructose-6-phosphate transaminase 1 (Gfpt1),
            mRNA.
ACCESSION   NM_013528
VERSION     NM_013528.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Holland,S.H., Carmona-Martinez,R., O'Connor,K., O'Neil,D., Roos,A.,
            Spendiff,S. and Lochmuller,H.
  TITLE     A Deficiency in Glutamine-Fructose-6-Phosphate Transaminase 1
            (Gfpt1) in Skeletal Muscle Results in Reduced Glycosylation of the
            Delta Subunit of the Nicotinic Acetylcholine Receptor (AChRdelta)
  JOURNAL   Biomolecules 14 (10), 1252 (2024)
   PUBMED   39456185
  REMARK    GeneRIF: A Deficiency in Glutamine-Fructose-6-Phosphate
            Transaminase 1 (Gfpt1) in Skeletal Muscle Results in Reduced
            Glycosylation of the Delta Subunit of the Nicotinic Acetylcholine
            Receptor (AChRdelta).
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Zhang,R., Farshadyeganeh,P., Ohkawara,B., Nakajima,K., Takeda,J.I.,
            Ito,M., Zhang,S., Miyasaka,Y., Ohno,T., Mori-Yoshimura,M.,
            Masuda,A. and Ohno,K.
  TITLE     Muscle-specific lack of Gfpt1 triggers ER stress to alleviate
            misfolded protein accumulation
  JOURNAL   Dis Model Mech 17 (8) (2024)
   PUBMED   38903011
  REMARK    GeneRIF: Muscle-specific lack of Gfpt1 triggers ER stress to
            alleviate misfolded protein accumulation.
REFERENCE   3  (bases 1 to 6248)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 6248)
  AUTHORS   Farshadyeganeh,P., Nazim,M., Zhang,R., Ohkawara,B., Nakajima,K.,
            Rahman,M.A., Nasrin,F., Ito,M., Takeda,J.I., Ohe,K., Miyasaka,Y.,
            Ohno,T., Masuda,A. and Ohno,K.
  TITLE     Splicing regulation of GFPT1 muscle-specific isoform and its roles
            in glucose metabolisms and neuromuscular junction
  JOURNAL   iScience 26 (10), 107746 (2023)
   PUBMED   37744035
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6248)
  AUTHORS   Werlen,G., Li,M.L., Tottone,L., da Silva-Diz,V., Su,X., Herranz,D.
            and Jacinto,E.
  TITLE     Dietary glucosamine overcomes the defects in alphabeta-T cell
            ontogeny caused by the loss of de novo hexosamine biosynthesis
  JOURNAL   Nat Commun 13 (1), 7404 (2022)
   PUBMED   36456551
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6248)
  AUTHORS   Tang,J., Neidigh,J.L., Cooksey,R.C. and McClain,D.A.
  TITLE     Transgenic mice with increased hexosamine flux specifically
            targeted to beta-cells exhibit hyperinsulinemia and peripheral
            insulin resistance
  JOURNAL   Diabetes 49 (9), 1492-1499 (2000)
   PUBMED   10969833
REFERENCE   7  (bases 1 to 6248)
  AUTHORS   Huynh,Q.K., Gulve,E.A. and Dian,T.
  TITLE     Purification and characterization of glutamine:fructose 6-phosphate
            amidotransferase from rat liver
  JOURNAL   Arch Biochem Biophys 379 (2), 307-313 (2000)
   PUBMED   10898949
REFERENCE   8  (bases 1 to 6248)
  AUTHORS   Oki,T., Yamazaki,K., Kuromitsu,J., Okada,M. and Tanaka,I.
  TITLE     cDNA cloning and mapping of a novel subtype of
            glutamine:fructose-6-phosphate amidotransferase (GFAT2) in human
            and mouse
  JOURNAL   Genomics 57 (2), 227-234 (1999)
   PUBMED   10198162
REFERENCE   9  (bases 1 to 6248)
  AUTHORS   Sayeski,P.P., Wang,D., Su,K., Han,I.O. and Kudlow,J.E.
  TITLE     Cloning and partial characterization of the mouse
            glutamine:fructose-6-phosphate amidotransferase (GFAT) gene
            promoter
  JOURNAL   Nucleic Acids Res 25 (7), 1458-1466 (1997)
   PUBMED   9060444
REFERENCE   10 (bases 1 to 6248)
  AUTHORS   Sayeski,P.P., Paterson,A.J. and Kudlow,J.E.
  TITLE     The murine glutamine:fructose-6-phosphate amidotransferase-encoding
            cDNA sequence
  JOURNAL   Gene 140 (2), 289-290 (1994)
   PUBMED   8144040
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB664274.1, AK019852.1, AC162465.3 and CN664418.1.
            
            On Sep 19, 2008 this sequence version replaced NM_013528.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK019852.1, BC050762.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 BB664274.1         2-5
            5-2412              AK019852.1         1-2408
            2413-5631           AC162465.3         36825-40043
            5632-6248           CN664418.1         1-617
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 37.81 cM"
     gene            1..6248
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /note="glutamine fructose-6-phosphate transaminase 1"
                     /db_xref="GeneID:14583"
                     /db_xref="MGI:MGI:95698"
     exon            1..160
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     exon            161..268
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     exon            269..376
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     exon            377..502
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     exon            503..561
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     exon            562..696
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     exon            697..758
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     exon            759..838
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     exon            839..944
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1108
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1204
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1302
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..1423
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     exon            1424..1581
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     exon            1582..1696
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1824
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     exon            1825..1992
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2154
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     exon            2155..6248
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6219..6224
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /note="hexamer: AATAAA"
     polyA_site      6238
                     /gene="Gfpt1"
                     /gene_synonym="2810423A18Rik; GFA; GFAT; GFAT1; GFAT1m;
                     Gfpt"
                     /note="major polyA site"
ORIGIN      
        1 agttgagagc gaagcgagcg ctgagtcgga ctgtcgggtc tgagctgtcg catcccagag
       61 tcctctcatt gccaccaccc cggcccgagc tcaccctcgc ttctgaagct ctccgcgcgc
      121 ccgacagctc agccctcgcc cgtgaccaac atcatgtgcg gtatatttgc ttatttaaat
      181 taccatgttc ctcgaacaag acgagaaatc ttggagacac taatcaaagg ccttcagaga
      241 ctggaataca gaggatatga ttctgctggt gtgggacttg acggaggcaa tgacaaagac
      301 tgggaagcca acgcctgcaa aatccagctc attaagaaga aaggaaaagt taaggcactg
      361 gatgaagaag ttcacaaaca acaagatatg gacttggata tagaatttga tgtgcatctt
      421 ggaatagctc atacccgttg ggcgacacat ggagaaccca atcctgtcaa tagtcacccc
      481 cagcgctctg ataaaaataa tgaattcatt gttattcata atggaatcat caccaactac
      541 aaagacttga aaaagtttct ggaaagcaaa ggctatgact ttgaatctga aacagacaca
      601 gaaaccattg ccaagctcgt caagtacatg tatgacaact gggagagcca ggacgtcagt
      661 tttaccacct tggtggagag agttatccaa caattggaag gcgcctttgc tcttgtgttt
      721 aaaagtgtcc attttcccgg gcaagcagtt ggcacaaggc gaggtagccc tctcttgatt
      781 ggtgtgcgga gtgaacataa gctttctaca gatcacattc cgattctgta cagaacaggc
      841 aaagacaaga aaggaagctg cggtctttcc cgtgtggaca gcacgacatg cctgttccct
      901 gttgaggaaa aggcagttga atattacttt gcttctgatg caagtgccgt gatagagcac
      961 accaatcgtg tcatctttct ggaagatgat gatgttgcag cagtggtgga tggccgtctc
     1021 tctatccacc gaattaaacg aactgcagga gaccatcctg gccgagctgt gcaaactctc
     1081 cagatggagc tccagcagat catgaagggc aactttagtt catttatgca gaaggaaatt
     1141 tttgagcagc cagaatctgt tgtgaacaca atgagaggaa gagtcaattt tgatgactac
     1201 actgtgaatt tgggaggttt gaaagatcac attaaggaga tccagcggtg tcggcggttg
     1261 attcttattg cttgtggcac aagttaccac gctggtgtgg caacccgtca ggtcctggag
     1321 gagctgaccg agctgcccgt gatggtggag cttgccagtg acttcttgga tagaaacact
     1381 ccagtctttc gagatgatgt ttgctttttc attagtcaat caggcgagac agctgacacc
     1441 ctgatgggac ttcgttactg taaggagaga ggagccttaa ctgtggggat cacaaataca
     1501 gtcggcagtt ctatatcaag ggagacagat tgcggggttc atattaatgc tggtcctgag
     1561 attggcgtgg ccagtacaaa ggcatacacc agccagtttg tgtccctcgt gatgtttgct
     1621 ctcatgatgt gtgatgacag gatctccatg caagagagac gcaaagagat catgctcgga
     1681 ctgaagcgac tgccggactt gattaaggaa gtgctgagca tggatgatga aatccagaag
     1741 ctggcgacgg agctttacca ccagaagtcg gtcctgataa tggggcgggg ctaccattat
     1801 gctacatgcc ttgaaggggc tctgaaaatc aaggagatta cttatatgca ttcggaaggc
     1861 atccttgctg gtgagctcaa gcacggccct ctggccttgg tggacaagtt gatgcctgtc
     1921 atcatgatca tcatgcgaga ccacacttat gccaagtgcc agaacgctct tcagcaggtg
     1981 gttgcacggc aggggcgtcc agtcgtgatc tgtgataagg aggatactga gaccattaag
     2041 aatacaaaaa ggacaatcaa ggtgccccac tcagtggact gcttgcaggg cattctcagt
     2101 gtgattcccc tgcagctgct ggctttccac ctggctgtgc tgagaggcta cgatgttgat
     2161 tttccacgga atcttgccaa atctgtaaca gtagagtaac agacacctga aacttaagac
     2221 agttaagcaa cacgagatac cttttgtatt taaatttttg atttaaacta ttgcctcctg
     2281 aaagcctttt tttagtaaat cttatttata tatcagttat aattattcca ctcaactaat
     2341 gatttttgtg caattgtctc attttcaata atacatgggg gaatttgatt catagaattt
     2401 aagtctaaac ctaaaatcag agagatctca gctgtatttt gttcagagaa ctggataatg
     2461 gatttctggg ccctccattt catagaggag gcttggtgag tgtcaaggcg cttggtgttt
     2521 gcttcagcat aacgtgattc ggtcagtcag actgagggaa gaccagtgca ttgaattgga
     2581 tactccaagt cagtgtgtgc cagtgcctgg acagccacca aaggctttgc tgcgctactt
     2641 ataaatagaa gatgctgcag tttatgttga agcttatttc tgttttgttt ttaaatcaaa
     2701 ctgtacacca aaaaacttgg tgagattttt atcttagttt agttaaatta gttcaaacat
     2761 gtctgtcatc tctgtagaca gtgcgtttgg gtcctccaca cctgcagaga agaccttgtg
     2821 gagtttggaa aacttgtgct gttagacatt gatatgtggc tggacatttg aagataggac
     2881 acagggatcc actccttatt tcttcatctc ttaagagtag ttggcatttt ctcgcaactg
     2941 tagagcttaa ctgaaaagca agtactgttt gttgagtgac gtactttgta gctgcctttg
     3001 gcatcatagc acagagttgc tgtcaagcct caaattggtg tcagggtgct caattctctc
     3061 ttcagctttg tcccaagaag agtttcctgc agttaccttt acttcctccc ctgccttgtt
     3121 gcagaaagca tcccttggtg ccagtgtaga agttctgctt ggggggtggc aggccttgct
     3181 gctcatttga ccctcagtgt atgtggcagg ctcagctgag aacggcacat acttggcact
     3241 gtggagtgac gtcagtttca gggttgtttt ctcgatgttt tccttgggac tgttttgact
     3301 cctgttgagg agtgaatgca gtataaatac gtgagactgt catgccttca gctgattgtg
     3361 tcagaaatgc aggtcagcaa gttggataaa atatttaaaa tagaacatta gtttgacacc
     3421 ccaaaaatgt gcagagtaat ttcttcacag agctatttgg gtcagattgt gtgggcagtg
     3481 ggcaggtgtg tgtgttcaag ttaggtccat tgttttcaaa agctttcacc tgtacacggt
     3541 gaaagcgcag tgcttcacgg agtcatgctt tgtgtttgaa cttagtggta agcagttaac
     3601 acttgtgaag gtgagttgaa gaaaaagttg atgtcacttt aggcatttca gaaggcttca
     3661 ttcacactga tggaggctgg aaacctgatt ttgataaccc aagacagggc cacagtttgt
     3721 actccatgtc actgctttgg gtacgtttct ctgttgtatt tgtccctaag acttcatgtt
     3781 agtggagcat gtgtgtaagg tcatttctta ggcctgatgt ggtagcacag ctgctcgtgt
     3841 ttgggtgtgg cttttgtttg gtgtgtggct ctggagcaca gggtgatgtt ggtgtgttga
     3901 gacactgaaa ctgggagacg atcagctcag tgctgtctgg tcactcactg tcagctcagt
     3961 cactaccccg gggtcctgtt ggagcagttt tggattatac agctcagtct tgctaaccac
     4021 cctccgtgca gcggaacgtc acaactcttt ttgtcttctg tctatgatat tgaacccatt
     4081 taccagtttc tcatccatac acactccaag ctagaataaa tactaaacca gtcagaaacc
     4141 cagatgagga ccagggtagt actgtctaaa caaatattat tgttttccat atcccatttt
     4201 gctttctacc taaattcttt tattagttct taatacccag atattttagc aaggcaaggc
     4261 actaggtgag taggtcccac taatttggta taatacagga catagaaatc taatttttca
     4321 aaacaaaaat accctgtaaa agggaggctt tctattaaaa ttctcccccc cccctacccc
     4381 cagctccctg tttttcaaga cagggtttct ctgtgcagtc ctggctgaac tggaactcac
     4441 tctgtagatt aggctggcct cgaactcaca gagatctccc tacctctgcc tccagagtgc
     4501 tgtcattaaa ttctaatcta tctatctatc tttctttctt tctaaaaagt aattatttta
     4561 tttgtttaca tttcaaatgt cctccttcct ggtttcccct ccatgaacct cccaccccat
     4621 ccccctcctt tttgcctcta agagggtgct ccccaccccc tccgcacccc ctagcatctc
     4681 ccttctctgg ggcatcaggc cgccacagga ccgagcacat ttatagtgtg tttaagttca
     4741 cacacacatc tttacctggg agtccttgct aggcagggag gactgaggca cctgacacat
     4801 ggcccacttg atggttacta ctttctaaaa aagagaatct atgccgtaag atagaaaggt
     4861 tagaaggtga gccactcact cttagcctgt gactggctcg gcggctgtgt atgagcggct
     4921 gccatgctgg gcagcagagc tagaccctca gagcaattgt gactgtccct ggtggacctg
     4981 gtccttgtga ggctcaggag ggagggtgga aaggtggagc tgtgctgagg tattaaatgc
     5041 tgggcctggg ttcgtttgac atgttcataa gcagctgccc cggcctgtcc gtggaacagg
     5101 ggactataaa gaacagcagt tagtaatatg gcaagcagtt gtgtgtccct gctcagcaga
     5161 gtgatgtcac agggtactgt gcagaaggct gttagttcag aacaccaaca tcagcttttt
     5221 aatagcaggc tgtttctgaa gtgacaggtg gtaacccttt cagtccaggc ctcttgcctc
     5281 agtggttctg aaggtgggag gaaaggaggc tggacttggt cccatgtaga gggaaactaa
     5341 cactttcaca acacaccttc agtctttggg gttactcttt tatctttttt tataattagg
     5401 atattctaga gcaataaaag agactaaggt tacctgttta ttatatacat taactggtat
     5461 gagttttcta aaaaaaaata ataaagcaca atgtcattgg aaaatgtttg tttaaaaaaa
     5521 gaattcataa tttacaactt gggaaggcca aagacatgga agggaaaggt aaagacattt
     5581 tgatttatga catccacatt ttgatttatg acaacccata gtgggttgtg tgtacagtaa
     5641 tactacgcag ggttttaagc ttggagacat ttcacatggc ttttatttag tttcacattc
     5701 aaaagtttta acataaattg gactttttca ctgtacttaa agattgttaa taagtatttc
     5761 agtatcttca tagctagaat ttatttaaac atatcctcag atctgggatg ataacagata
     5821 ataaaactga cagcattggc tttgaatagt aatgtccata gttttaagca agactgctaa
     5881 gttgtgttgt gttgactgta agtccgtacg ctcagtagtg atgttctgaa ggctgatgat
     5941 ggcatcagag gaaacgtcac cttatcttgt ttctgcacta gtttccagag agtgtagaaa
     6001 tggtcttgct aatttttgaa tgttctttgg ttgaatgtgt ttttccatta ataacatttt
     6061 tatatttgtt taaattatac ttctgtggga ctgggggtta gaataatcat tgctctgttt
     6121 tgattttgta ttcttttaaa tgaatgttta gttttctgag ccagatggtt atttcatcct
     6181 tgtgtcctgt tcaatccagt gactacaccg gaactgtgaa taaattaccc aaaaccttgc
     6241 tgtgtgac
//
