LOCUS       NM_177266               3198 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus G elongation factor, mitochondrial 2 (Gfm2),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_177266
VERSION     NM_177266.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Han,W., Peng,B.Z., Wang,C., Townsend,G.E. 2nd, Barry,N.A.,
            Peske,F., Goodman,A.L., Liu,J., Rodnina,M.V. and Groisman,E.A.
  TITLE     Gut colonization by Bacteroides requires translation by an EF-G
            paralog lacking GTPase activity
  JOURNAL   EMBO J 42 (2), e112372 (2023)
   PUBMED   36472247
  REMARK    GeneRIF: Gut colonization by Bacteroides requires translation by an
            EF-G paralog lacking GTPase activity.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK044371.1, AC154620.2 and AK035819.1.
            
            On Sep 28, 2012 this sequence version replaced NM_177266.4.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1). Variants 1 and 3 encode the same isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC145208.1, SRR9219382.94160.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                AK044371.1         2-66
            66-123              AC154620.2         43287-43344         c
            124-1330            AK035819.1         30-1236
            1331-1331           AC154620.2         18387-18387         c
            1332-3012           AK035819.1         1237-2917
            3013-3198           AC154620.2         4895-5080           c
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 50.66 cM"
     gene            1..3198
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /note="G elongation factor, mitochondrial 2"
                     /db_xref="GeneID:320806"
                     /db_xref="MGI:MGI:2444783"
     exon            1..123
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            124..207
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     CDS             145..2484
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ribosome-releasing factor 2, mitochondrial"
                     /codon_start=1
                     /product="ribosome-releasing factor 2, mitochondrial
                     isoform 1"
                     /protein_id="NP_796240.3"
                     /db_xref="CCDS:CCDS26708.2"
                     /db_xref="GeneID:320806"
                     /db_xref="MGI:MGI:2444783"
                     /translation="MFTKWRIFAVNHQRTFSVHLNTMCYCKIKANLKRLKTQLPLTRN
                     YSSAPGIAGSDVKSLHSVINPPVAKIRNIGIMAHIDAGKTTTTERILYYSGYTRSLGD
                     VDDGDTVTDFMAQERERGITIQSAAVTLDWKGYRVNLIDTPGHVDFTLEVERCLRVLD
                     GAVAVFDASAGVEAQTLTVWRQADKHKIPRICFLNKMDKTGASFNYAVESIREKLKAK
                     PLILQLPIGEARTFQGVVDVVNKEKLLWNSNSDDGKDFERMPLSEASDRELLKETIEA
                     RNSLIEQVADLDDEFADLVLGEFSENFDLVPAEKLQAAVHRVTLAQAAVPVLCGSALK
                     NKGVQPLLDAVTTYLPSPEEREDRFLQWYEGDLCALAFKVLHDKQRGPLVFLRIYSGT
                     LTPQLAVHNINRNCTERMSRLLLPFADQHVEIPSLTAGNIALTVGLKQTATGDTIVSS
                     KSSALAAARRAGRGEREHGKKREAESLLLAGVEVPEPVFFCTIEPPSVAKQPDLDHAL
                     ERLQREDPSLKVKLDPDSGQTVLCGMGELHIEIIHDRIKREYGLETYLGPLQVAYRET
                     ILNSVRATDTLDRVLGDKRHLVSAELEVRPAEEPCAVAKIEYADCVGEDLLQASREAI
                     ESAVHSACLQGPLLGSPVQDVAMTLHSLMIHPGTSTTMVTACISRCMQKALKKADKQV
                     LEPLMSLEVTVSREYLSPVLADLAQRRGNIQEIQTRQDNRVVLGFVPLAEIMGYSTVL
                     RTLTSGSATFALELSTYQAMSPQDQSALLNQRSGLAHVL"
     exon            208..292
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            293..350
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            351..448
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            449..574
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            575..663
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            664..752
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            753..813
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            814..993
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1074
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            1075..1223
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1364
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            1365..1464
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            1465..1651
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            1652..1728
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            1729..1867
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            1868..2047
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            2048..2163
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            2164..2346
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     exon            2347..3198
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2559..2564
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /note="hexamer: AGTAAA"
     polyA_site      2587
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
                     /note="major polyA site"
     polyA_site      2618
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
     polyA_site      3018
                     /gene="Gfm2"
                     /gene_synonym="6530419G12Rik; A930009M04Rik; EF-G2mt;
                     EFG2; MST027; RRF2mt"
ORIGIN      
        1 aggcagatgg gcggaaattg acagcaggag aattttagct gagacgccac cggaactgct
       61 tagagggcgg aaggtgctta tggagctttt gtaagctggg agatccgcgg gttccttctg
      121 cggagaacgt tttagaggac cacgatgttc accaaatgga ggatttttgc agtgaatcat
      181 cagagaacgt tcagtgtgca ccttaatacc atgtgttact gcaaaataaa agcaaattta
      241 aaaagattaa agactcagct gcctcttaca agaaactaca gttctgcacc aggcatagca
      301 ggaagtgatg tcaagtctct tcattccgtc attaaccctc ctgtggccaa aattcgtaat
      361 attggaatta tggcacatat tgatgcaggc aaaaccacca ccacagagag aatcctgtac
      421 tattctggat acacaagatc attgggagat gtcgatgatg gagacacagt gacggatttc
      481 atggctcaag agcgagaaag gggcattacc atccagtcag cagctgtgac tctcgactgg
      541 aaagggtata gagtcaatct gattgatact ccaggtcacg tagacttcac tctggaggtc
      601 gagcgctgct tgagggtgtt ggacggtgcg gtggccgtgt ttgacgcctc tgctggtgta
      661 gaggcccaga ctctcactgt atggaggcaa gctgacaaac acaaaatacc ccggatctgc
      721 tttttaaaca agatggacaa gactggagca agttttaact atgcggttga aagcatccgg
      781 gagaagttaa aggcaaagcc cctgatttta cagctgccaa ttggtgaagc cagaactttc
      841 caaggagtgg tggatgtcgt aaacaaagaa aaactccttt ggaattctaa ctctgacgat
      901 ggaaaagact tcgagaggat gcccctctca gaagcgagtg atcgggaact gctaaaggag
      961 acaattgaag caaggaactc cttaattgaa caagttgcag acttagatga tgagtttgct
     1021 gacttggttt taggtgaatt tagtgaaaac tttgatttag taccagctga aaagttacag
     1081 gctgcagtac acagggtgac actggcacag gctgcagtgc ctgtgctgtg tgggagtgcc
     1141 ttgaagaaca agggcgtgca gcccctgctg gatgcggtga ccacttacct gccgtcaccc
     1201 gaggagcgcg aggaccgctt tctgcagtgg tatgagggtg acttgtgtgc actagcgttt
     1261 aaagtactac atgacaaaca gcgaggaccc ctggttttcc tgcgcattta ctcgggaaca
     1321 ctgacgcctc agctggctgt ccacaacatt aatagaaact gcacggaaag aatgagtcgt
     1381 ctgcttttgc catttgctga ccaacatgta gaaatccctt ccctgactgc tggaaacatt
     1441 gctttgacag ttgggcttaa acaaactgcc actggagaca ccatagtctc gtccaagtcc
     1501 agcgcattag ctgcagctcg cagagccgga cgaggagaaa gggagcatgg aaagaagaga
     1561 gaggccgaga gtctgctgtt ggccggggtg gaggtcccag agcctgtctt cttctgcacc
     1621 atagagcccc cgtcagtcgc taagcagcca gatttagatc atgccttgga acgccttcag
     1681 cgcgaagatc ccagtttgaa agtgaaactc gatcctgact ctggacaaac tgtcctgtgt
     1741 ggaatggggg agttgcatat tgagattatt catgaccgta tcaagaggga gtatggccta
     1801 gagacctatc tagggcctct gcaggtggca taccgggaaa ccatcttaaa ctcagttcgc
     1861 gccacagaca ccctcgatag ggtgcttgga gacaagcggc acttggtgag tgcggagctg
     1921 gaagtccggc cagcagaaga gccctgtgct gtggccaaga tcgagtatgc tgactgtgtg
     1981 ggtgaagacc tgctgcaggc ctctcgggag gccattgaaa gtgccgtcca cagtgcatgc
     2041 ctccagggac ctctgctcgg atccccagtc caggatgtgg caatgacgtt gcactcactg
     2101 atgattcacc ctggcacctc cacaactatg gtcactgcct gcatctcacg gtgtatgcag
     2161 aaggccctga agaaagctga caaacaggtg ctggaacctc tgatgagtct cgaggtcaca
     2221 gtgagcaggg agtacctcag ccctgtcctg gcagacttgg cacaaagaag agggaacatt
     2281 caggaaatcc agactcgcca agacaacaga gttgttctag gattcgttcc tctagcagag
     2341 attatgggat attcaactgt gcttcgaact ctgacatctg gctcagctac ttttgcctta
     2401 gaactgtcta cttatcaagc catgagtcct caagaccaga gtgctctact caaccagaga
     2461 agtggcttgg cccatgtgct ataggccctt ggaaacgggg aaagctgggc cagttgcctg
     2521 ctttattccc ggcaagaatg ctgttttatt aactggctag taaattgctg ctttggtgtg
     2581 gaaagaactt gacaagaccc ttggagctgc cattctaatt ctgttgggtg tactttgcta
     2641 tattgcacca cagctgggcc ccggttatct ctgccatctc tggtgctggg ataaaataac
     2701 ctggccaggg cagcttagga aaaaaaaaag gtttcttttc tcagcaggaa gcaaggcaat
     2761 gagctctcaa agtccaccta gtgatgaact tcctgaagcc aagatacact gcctaagcct
     2821 ccccaaacag caccaccccc gggagcagag tccagaagcc tgggcctgtg aggagcggac
     2881 ctcgtttaga catttcttgt tttacttttg agatgttcca tccccctgga gctggaattg
     2941 tggtgtgatt acggagatcc agggcctgag ctcgggtcct ctgcaagagt gatcttaaat
     3001 acctcagcct tctttctagc ctgacatctc tcttttaaac cattgcaaga tgacagtatc
     3061 cacacagggc agccctctgg agtgctagtg caaggctttt ccttttattt tcttaagctg
     3121 ctcacataaa agattcagtg tttgattcag ggatagaaag attcaatcaa taaaaagatg
     3181 gtttatttaa aaaaaaaa
//
