LOCUS       NM_138591               2598 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus G elongation factor, mitochondrial 1 (Gfm1), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_138591
VERSION     NM_138591.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Molina-Berenguer,M., Vila-Julia,F., Perez-Ramos,S.,
            Salcedo-Allende,M.T., Camara,Y., Torres-Torronteras,J. and Marti,R.
  TITLE     Dysfunctional mitochondrial translation and combined oxidative
            phosphorylation deficiency in a mouse model of hepatoencephalopathy
            due to Gfm1 mutations
  JOURNAL   FASEB J 36 (1), e22091 (2022)
   PUBMED   34919756
  REMARK    GeneRIF: Dysfunctional mitochondrial translation and combined
            oxidative phosphorylation deficiency in a mouse model of
            hepatoencephalopathy due to Gfm1 mutations.
REFERENCE   3  (bases 1 to 2598)
  AUTHORS   Almeida,L., Dhillon-LaBrooy,A., Castro,C.N., Adossa,N.,
            Carriche,G.M., Guderian,M., Lippens,S., Dennerlein,S., Hesse,C.,
            Lambrecht,B.N., Berod,L., Schauser,L., Blazar,B.R., Kalesse,M.,
            Muller,R., Moita,L.F. and Sparwasser,T.
  TITLE     Ribosome-Targeting Antibiotics Impair T Cell Effector Function and
            Ameliorate Autoimmunity by Blocking Mitochondrial Protein Synthesis
  JOURNAL   Immunity 54 (1), 68-83 (2021)
   PUBMED   33238133
REFERENCE   4  (bases 1 to 2598)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2598)
  AUTHORS   Bailey,P.J., Klos,J.M., Andersson,E., Karlen,M., Kallstrom,M.,
            Ponjavic,J., Muhr,J., Lenhard,B., Sandelin,A. and Ericson,J.
  TITLE     A global genomic transcriptional code associated with CNS-expressed
            genes
  JOURNAL   Exp Cell Res 312 (16), 3108-3119 (2006)
   PUBMED   16919269
REFERENCE   6  (bases 1 to 2598)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   7  (bases 1 to 2598)
  AUTHORS   Gao,J., Yu,L., Zhang,P., Jiang,J., Chen,J., Peng,J., Wei,Y. and
            Zhao,S.
  TITLE     Cloning and characterization of human and mouse mitochondrial
            elongation factor G, GFM and Gfm, and mapping of GFM to human
            chromosome 3q25.1-q26.2
  JOURNAL   Genomics 74 (1), 109-114 (2001)
   PUBMED   11374907
REFERENCE   8  (bases 1 to 2598)
  AUTHORS   Ko,M.S., Threat,T.A., Wang,X., Horton,J.H., Cui,Y., Wang,X.,
            Pryor,E., Paris,J., Wells-Smith,J., Kitchen,J.R., Rowe,L.B.,
            Eppig,J., Satoh,T., Brant,L., Fujiwara,H., Yotsumoto,S. and
            Nakashima,H.
  TITLE     Genome-wide mapping of unselected transcripts from extraembryonic
            tissue of 7.5-day mouse embryos reveals enrichment in the t-complex
            and under-representation on the X chromosome
  JOURNAL   Hum Mol Genet 7 (12), 1967-1978 (1998)
   PUBMED   9811942
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124190.4.
            
            On Mar 21, 2008 this sequence version replaced NM_138591.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC031772.1, AF315511.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849379
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-208               AC124190.4         83869-84076         c
            209-361             AC124190.4         82389-82541         c
            362-494             AC124190.4         80704-80836         c
            495-699             AC124190.4         78482-78686         c
            700-816             AC124190.4         75529-75645         c
            817-967             AC124190.4         73939-74089         c
            968-1122            AC124190.4         70482-70636         c
            1123-1207           AC124190.4         69334-69418         c
            1208-1345           AC124190.4         64375-64512         c
            1346-1447           AC124190.4         62791-62892         c
            1448-1504           AC124190.4         60465-60521         c
            1505-1642           AC124190.4         57481-57618         c
            1643-1725           AC124190.4         56221-56303         c
            1726-1888           AC124190.4         46222-46384         c
            1889-2033           AC124190.4         44581-44725         c
            2034-2194           AC124190.4         40568-40728         c
            2195-2248           AC124190.4         39585-39638         c
            2249-2598           AC124190.4         39123-39472         c
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 30.96 cM"
     gene            1..2598
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /note="G elongation factor, mitochondrial 1"
                     /db_xref="GeneID:28030"
                     /db_xref="MGI:MGI:107339"
     exon            1..208
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /inference="alignment:Splign:2.1.0"
     CDS             125..2380
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /note="G elongation factor 1; EF-Gmt; mEF-G 1; elongation
                     factor G1; elongation factor G 1, mitochondrial"
                     /codon_start=1
                     /product="elongation factor G, mitochondrial"
                     /protein_id="NP_613057.2"
                     /db_xref="CCDS:CCDS38449.1"
                     /db_xref="GeneID:28030"
                     /db_xref="MGI:MGI:107339"
                     /translation="MRLLRVAAALGRGPFPRVPAVLGWQGKQADWKTRRWCSSGPVPN
                     EKIRNIGISAHIDSGKTTLTERVLYYTGRIATMHEVKGKDGVGAVMDSMELERQRGIT
                     IQSAATYTMWKDININIIDTPGHVDFTIEVERALRVLDGAVLVLCAVGGVQCQTMTVS
                     RQMKRYNVPFLTFINKLDRMGSNPSRALQQMRSKLNHNAAFVQIPIGLEGDFKGIIDL
                     IEERAIYFDGDFGQIVRYDEIPAGLRAAAADHRQELIECVANSDEQLGELFLEEKIPS
                     VSDLKRAIRRATLSRSFTPVFLGSALKNKGVQPLLDAVLEYLPNPSEVQNYAILNQND
                     SKEKTKILMNPQRDDSHPFVGLAFKLEAGRFGQLTYVRNYQGELKKGSTIYNTRTGKK
                     VRVQRLVRMHADMMEDVEEVYAGDICALFGIDCASGDTFTNKDNSDLSMESIHVPEPV
                     ISIAMRPSNKNDLEKFSKGIGRFTREDPTFKVHFDPESKETIVSGMGELHLEIYAQRM
                     EREYGCPCITGKPKVAFRETIVAPVPFDFTHKKQSGGAGQFGKVIGVLEPLPPEDYTK
                     LEFSDETFGSNVPKQFVPAVEKGFLDACEKGPLSGHKLSGLRFVLQDGAHHMVDSNEI
                     SFIRAGEGALKQALANGTLCIIEPIMSVEVIAPNEFQGTVFAGINRRHGVITGQDGIE
                     DYFTLYADVPLNNMFGYSTELRSCTEGKGEYTMEYCRYQPCSPSTQEELINKYLEATG
                     QLPVKKGKAKN"
     exon            209..361
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /inference="alignment:Splign:2.1.0"
     exon            362..494
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /inference="alignment:Splign:2.1.0"
     exon            495..699
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /inference="alignment:Splign:2.1.0"
     exon            700..816
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /inference="alignment:Splign:2.1.0"
     exon            817..967
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1122
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1207
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1345
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1447
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1504
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /inference="alignment:Splign:2.1.0"
     exon            1505..1642
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1725
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..1888
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /inference="alignment:Splign:2.1.0"
     exon            1889..2033
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /inference="alignment:Splign:2.1.0"
     exon            2034..2194
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /inference="alignment:Splign:2.1.0"
     exon            2195..2248
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /inference="alignment:Splign:2.1.0"
     exon            2249..2598
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2577..2582
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /note="hexamer: AATAAA"
     polyA_site      2598
                     /gene="Gfm1"
                     /gene_synonym="D3Wsu133e; Gfm"
                     /note="major polyA site"
ORIGIN      
        1 cggaagtgct cctgcggctc cggttgccgg cgtgactttg agctcttccc gctctgctgc
       61 cggcgctgag cacaaccgat cacctcagag caggagcctt ggaagtggct gttccaggct
      121 tgccatgcgg ctgctgcggg tcgccgcagc cctggggcgt ggaccgttcc ctcgggtccc
      181 tgcagtcctg ggctggcagg ggaagcaggc tgattggaag acccgccgat ggtgttcgtc
      241 agggccagtt cccaatgaaa aaatacggaa cattgggatc tcagctcaca ttgattctgg
      301 gaagacgacg ctaacagaac gggtgctata ctacactggc aggattgcca cgatgcacga
      361 ggtgaaaggt aaagatggag ttggcgctgt catggattcc atggagctgg agaggcagcg
      421 agggatcacg atccagtcag cagctacgta taccatgtgg aaagacatca atatcaacat
      481 catagacacg ccaggccacg tggactttac catagaagtg gaaagagccc tgcgggtcct
      541 ggacggtgca gtccttgttc tctgtgcggt tggcggagtc cagtgccaga ccatgacagt
      601 cagtcgtcag atgaagcgct acaacgttcc ctttttaacc ttcattaaca aactggaccg
      661 aatgggctct aacccatcca gggccctcca acaaatgaga tctaaactaa atcacaatgc
      721 tgcatttgta caaataccca ttggtttaga gggtgatttt aagggaatta tagacctcat
      781 tgaagaacga gctatttact ttgatggaga ctttggtcag attgtccgat atgacgagat
      841 tccggctgga ttgcgggccg cagccgctga ccaccggcag gagctcattg agtgcgttgc
      901 taactcagat gagcagcttg gtgagctctt cctggaagaa aagatcccct cagtttccga
      961 cttaaagcga gcaatccgga gagctactct gagtcgatcc ttcactcctg tatttttggg
     1021 aagtgctttg aaaaacaaag gagttcaacc tcttttggac gctgttttag aatacctgcc
     1081 caatccatct gaggtccaga attacgctat actcaatcag aatgactcaa aagagaagac
     1141 caaaatctta atgaaccccc aaagagatga ttcgcaccca tttgtaggcc tggcttttaa
     1201 actggaggcg ggccgttttg gacagctgac ttatgtccga aattatcaag gtgaactgaa
     1261 gaagggaagc accatctaca acacgaggac cgggaagaaa gtgcgcgtgc agcggctagt
     1321 gcgcatgcac gccgacatga tggaggatgt tgaagaagta tatgctggag acatctgtgc
     1381 attgtttggc attgactgtg caagtggaga tacattcaca aacaaagata acagtgacct
     1441 ttctatggaa tcgattcatg ttcctgagcc tgtcatttca atagcaatga ggccttctaa
     1501 caagaatgat ctggagaaat tttccaaagg tattggcagg tttacacggg aagaccccac
     1561 atttaaagtc cactttgacc ctgagagcaa ggagaccatt gtgtcaggga tgggagagct
     1621 gcacctggaa atctatgctc agagaatgga gagagaatat ggctgccctt gtatcacagg
     1681 aaaacccaaa gtagccttta gagagaccat tgttgcccca gtgccgtttg attttacaca
     1741 taaaaagcag tcgggtggtg ccggccagtt tggaaaagta ataggagtgc tggagccctt
     1801 gcccccagag gactacacga agttggagtt ttctgatgag acatttgggt ccaatgttcc
     1861 aaagcagttt gtccccgctg ttgaaaaggg gtttctggat gcctgcgaaa aaggccctct
     1921 ttctggtcac aagctttctg ggctacggtt tgttctgcaa gatggagcac accacatggt
     1981 tgattcgaat gaaatctctt tcatccgagc tggagaaggt gctcttaaac aagccttggc
     2041 aaatggcaca ctatgtatta ttgagcctat tatgtctgtg gaagttatag cccccaatga
     2101 attccaggga acagtgttcg cagggattaa ccggcggcat ggagtgatta cagggcagga
     2161 cggcattgag gactacttca cactgtatgc agatgttccc ctaaataata tgtttggtta
     2221 ttccactgag cttaggtctt gcacagaggg aaaaggagag tacacgatgg agtactgcag
     2281 gtaccagccg tgttcaccat ccacacagga agagctcatc aataagtatt tggaagctac
     2341 aggtcagctt cccgtaaaaa aagggaaagc caagaactga ctctcctcac tcaggatata
     2401 ctgactattt gactaccaca cttggggatg atggagtctg cttgaatgaa ttctgacggc
     2461 agaaacaaat ttaggagccc ctgctggccc acgttcagga gcttctgtat gtacgccaag
     2521 gataactgtg tatttaaact gtattgacta tttttattgt ttagtaaaag accttaaata
     2581 aaattatatt attactga
//
