LOCUS       NM_019652               1264 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus guided entry of tail-anchored proteins factor 3,
            ATPase (Get3), transcript variant 1, mRNA.
ACCESSION   NM_019652
VERSION     NM_019652.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Norlin,S., Parekh,V. and Edlund,H.
  TITLE     The ATPase activity of Asna1/TRC40 is required for pancreatic
            progenitor cell survival
  JOURNAL   Development 145 (1) (2018)
   PUBMED   29180572
  REMARK    GeneRIF: These findings support a role for the Asna1 ATPase
            activity in ensuring the survival of pancreatic multipotent
            progenitor cells , possibly by counteracting p53-mediated
            apoptosis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Vogl,C., Panou,I., Yamanbaeva,G., Wichmann,C., Mangosing,S.J.,
            Vilardi,F., Indzhykulian,A.A., Pangrsic,T., Santarelli,R.,
            Rodriguez-Ballesteros,M., Weber,T., Jung,S., Cardenas,E., Wu,X.,
            Wojcik,S.M., Kwan,K.Y., Del Castillo,I., Schwappach,B.,
            Strenzke,N., Corey,D.P., Lin,S.Y. and Moser,T.
  TITLE     Tryptophan-rich basic protein (WRB) mediates insertion of the
            tail-anchored protein otoferlin and is required for hair cell
            exocytosis and hearing
  JOURNAL   EMBO J 35 (23), 2536-2552 (2016)
   PUBMED   27458190
REFERENCE   3  (bases 1 to 1264)
  AUTHORS   Norlin,S., Parekh,V.S., Naredi,P. and Edlund,H.
  TITLE     Asna1/TRC40 Controls beta-Cell Function and Endoplasmic Reticulum
            Homeostasis by Ensuring Retrograde Transport
  JOURNAL   Diabetes 65 (1), 110-119 (2016)
   PUBMED   26438609
  REMARK    GeneRIF: Asna1 ensures retrograde transport and ER and insulin
            homeostasis in beta-cells. Asna1 KO mice develop hypoinsulinemia,
            impaired insulin secretion, and glucose intolerance that rapidly
            progresses to overt diabetes.
REFERENCE   4  (bases 1 to 1264)
  AUTHORS   Favaloro,V., Spasic,M., Schwappach,B. and Dobberstein,B.
  TITLE     Distinct targeting pathways for the membrane insertion of
            tail-anchored (TA) proteins
  JOURNAL   J Cell Sci 121 (11), 1832-1840 (2008)
   PUBMED   18477612
REFERENCE   5  (bases 1 to 1264)
  AUTHORS   Mukhopadhyay,R., Ho,Y.S., Swiatek,P.J., Rosen,B.P. and
            Bhattacharjee,H.
  TITLE     Targeted disruption of the mouse Asna1 gene results in embryonic
            lethality
  JOURNAL   FEBS Lett 580 (16), 3889-3894 (2006)
   PUBMED   16797549
  REMARK    GeneRIF: These findings indicate that Asna1 plays a crucial role
            during early embryonic development.
REFERENCE   6  (bases 1 to 1264)
  AUTHORS   Bhattacharjee,H., Ho,Y.S. and Rosen,B.P.
  TITLE     Genomic organization and chromosomal localization of the Asna1
            gene, a mouse homologue of a bacterial arsenic-translocating ATPase
            gene
  JOURNAL   Gene 272 (1-2), 291-299 (2001)
   PUBMED   11470536
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY230823.1, BC083335.1 and BY631769.1.
            
            On Oct 24, 2017 this sequence version replaced NM_019652.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC016453.1, AF039405.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                BY230823.1         2-12
            12-1262             BC083335.1         1-1251
            1263-1264           BY631769.1         423-424
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 41.48 cM"
     gene            1..1264
                     /gene="Get3"
                     /gene_synonym="1810048H22Rik; ArsA; Asna1"
                     /note="guided entry of tail-anchored proteins factor 3,
                     ATPase"
                     /db_xref="GeneID:56495"
                     /db_xref="MGI:MGI:1928379"
     exon            1..183
                     /gene="Get3"
                     /gene_synonym="1810048H22Rik; ArsA; Asna1"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..1069
                     /gene="Get3"
                     /gene_synonym="1810048H22Rik; ArsA; Asna1"
                     /EC_number="7.3.2.7"
                     /note="isoform 1 is encoded by transcript variant 1;
                     arsenite-translocating ATPase; ATPase Asna1; ATPase GET3;
                     arsenite-stimulated ATPase; arsenical pump-driving ATPase"
                     /codon_start=1
                     /product="ATPase GET3 isoform 1"
                     /protein_id="NP_062626.1"
                     /db_xref="CCDS:CCDS40417.1"
                     /db_xref="GeneID:56495"
                     /db_xref="MGI:MGI:1928379"
                     /translation="MAAGVAGWGVEAEEFEDAPDVEPLEPTLSNIIEQRSLKWIFVGG
                     KGGVGKTTCSCSLAVQLSKGRESVLIISTDPAHNISDAFDQKFSKVPTKVKGYDNLFA
                     MEIDPSLGVAELPDEFFEEDNMLSMGKKMMQEAMSAFPGIDEAMSYAEVMRLVKGMNF
                     SVVVFDTAPTGHTLRLLNFPTIVERGLGRLMQIKNQISPFISQMCNMLGLGDMNADQL
                     ASKLEETLPVIRSVSEQFKDPEQTTFICVCIAEFLSLYETERLIQELAKCKIDTHNII
                     VNQLVFPDPEKPCKMCEARHKIQAKYLDQMEDLYEDFHIVKLPLLPHEVRGADKVNTF
                     SALLLEPYKPPSTQ"
     exon            184..331
                     /gene="Get3"
                     /gene_synonym="1810048H22Rik; ArsA; Asna1"
                     /inference="alignment:Splign:2.1.0"
     exon            332..480
                     /gene="Get3"
                     /gene_synonym="1810048H22Rik; ArsA; Asna1"
                     /inference="alignment:Splign:2.1.0"
     exon            481..631
                     /gene="Get3"
                     /gene_synonym="1810048H22Rik; ArsA; Asna1"
                     /inference="alignment:Splign:2.1.0"
     exon            632..739
                     /gene="Get3"
                     /gene_synonym="1810048H22Rik; ArsA; Asna1"
                     /inference="alignment:Splign:2.1.0"
     exon            740..937
                     /gene="Get3"
                     /gene_synonym="1810048H22Rik; ArsA; Asna1"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1264
                     /gene="Get3"
                     /gene_synonym="1810048H22Rik; ArsA; Asna1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1244..1249
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Get3"
                     /gene_synonym="1810048H22Rik; ArsA; Asna1"
                     /note="hexamer: AATAAA"
     polyA_site      1262
                     /gene="Get3"
                     /gene_synonym="1810048H22Rik; ArsA; Asna1"
                     /note="major polyA site"
ORIGIN      
        1 aatcacgtga gccgggtcca agatggcggc gggggtggcc gggtgggggg ttgaagcaga
       61 agagttcgag gatgcacctg atgtggagcc gctggaaccc acgcttagca atatcatcga
      121 gcagcgtagc cttaagtgga tcttcgtcgg gggcaagggt ggcgttggta agaccacctg
      181 cagctgcagc ctggcagtcc agctgtcgaa gggacgtgag agtgttctga tcatttccac
      241 agacccagct cacaacatct cagatgcttt tgaccagaag ttctccaagg tgcctaccaa
      301 ggtcaaaggc tatgacaacc tctttgctat ggagatagac cccagcctgg gcgttgcaga
      361 gctccccgac gagttcttcg aggaagacaa catgctgagc atgggcaaga agatgatgca
      421 ggaggccatg agcgccttcc ctggcatcga tgaagccatg agttatgctg aggtcatgag
      481 gctggtgaaa ggcatgaact tctcagtggt agtgttcgac acagcaccca ccggccatac
      541 actcaggctc ctgaacttcc cgaccatcgt ggagaggggc ctgggccgcc tgatgcagat
      601 caagaaccag atcagcccct tcatctcaca gatgtgcaac atgctgggcc tgggggacat
      661 gaatgctgac caactggcct ccaagctaga agagacgttg cctgtcatcc gatctgttag
      721 cgaacagttc aaggaccctg aacagacaac gttcatctgt gtgtgcatcg ccgaattctt
      781 gtccttgtac gagacggagc ggctgatcca ggagctggct aagtgcaaga tcgacaccca
      841 caacatcatc gtcaaccagc ttgtcttccc tgaccctgag aaaccctgca agatgtgtga
      901 ggcccgacac aagatccagg ccaagtacct ggaccagatg gaagacctat atgaagactt
      961 tcacattgta aagctgccac tgttacctca cgaggttcgg ggagccgaca aagtcaacac
     1021 cttctctgcc ctcctcctgg agccctacaa gccccccagc acccagtagc cacccccatc
     1081 cctgctgctg ccttcatacc ctcctctttc cccatggggc agagtttgca caaagtcccc
     1141 cataatgcag ggggccactt aggaggtggg agggggtctg ttccccctgg tggggcctgg
     1201 ggaggctgca atgcccccca cctcttcctg cctcacactc ctcaataaaa taatcttaca
     1261 cagc
//
