LOCUS       NM_177331               5291 bp    mRNA    linear   ROD 04-APR-2024
DEFINITION  Mus musculus GEN1, Holliday junction 5' flap endonuclease (Gen1),
            mRNA.
ACCESSION   NM_177331
VERSION     NM_177331.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Du,X., Yu,M., Ju,H., Xue,S., Li,Y., Wu,X., Xu,H. and Shen,Q.
  TITLE     Inhibition of MAPK/ERK pathway activation rescues congenital
            anomalies of the kidney and urinary tract (CAKUT) in Robo2PB/+
            Gen1PB/+ mice
  JOURNAL   Biochem Biophys Res Commun 653, 153-160 (2023)
   PUBMED   36870240
  REMARK    GeneRIF: Inhibition of MAPK/ERK pathway activation rescues
            congenital anomalies of the kidney and urinary tract (CAKUT) in
            Robo2[PB/+] Gen1[PB/+] mice.
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Fernandez,K.C., Feeney,L., Smolkin,R.M., Yen,W.F., Matthews,A.J.,
            Alread,W., Petrini,J.H.J. and Chaudhuri,J.
  TITLE     The structure-selective endonucleases GEN1 and MUS81 mediate
            complementary functions in safeguarding the genome of proliferating
            B lymphocytes
  JOURNAL   Elife 11, e77073 (2022)
   PUBMED   36190107
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5291)
  AUTHORS   Li,Y., Yu,M., Tan,L., Xue,S., Du,X., Wang,C., Wu,X., Xu,H. and
            Shen,Q.
  TITLE     Disruption of Gen1 causes ectopic budding and kidney hypoplasia in
            mice
  JOURNAL   Biochem Biophys Res Commun 589, 173-179 (2022)
   PUBMED   34922199
  REMARK    GeneRIF: Disruption of Gen1 causes ectopic budding and kidney
            hypoplasia in mice.
REFERENCE   4  (bases 1 to 5291)
  AUTHORS   Wang,X., Wang,H., Liu,J., Gong,Y., Zhang,C., Fang,F., Li,A., Wu,X.,
            Shen,Q. and Xu,H.
  TITLE     Gen1 mutation caused kidney hypoplasia and defective ureter-bladder
            connections in mice
  JOURNAL   Int J Biol Sci 16 (9), 1640-1647 (2020)
   PUBMED   32226308
  REMARK    GeneRIF: Gen1 mutation caused kidney hypoplasia and defective
            ureter-bladder connections in mice.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5291)
  AUTHORS   Zhang,Y., Zhang,X., Wang,X., Wang,H., Wu,X., Xu,H. and Shen,Q.
  TITLE     Gen1 Modulates Metanephric Morphology Through Retinoic Acid
            Signaling
  JOURNAL   DNA Cell Biol 38 (3), 263-271 (2019)
   PUBMED   30632787
REFERENCE   6  (bases 1 to 5291)
  AUTHORS   Zhou,L., Dai,H., Wu,J., Zhou,M., Yuan,H., Du,J., Yang,L., Wu,X.,
            Xu,H., Hua,Y., Xu,J., Zheng,L. and Shen,B.
  TITLE     Role of FEN1 S187 phosphorylation in counteracting oxygen-induced
            stress and regulating postnatal heart development
  JOURNAL   FASEB J 31 (1), 132-147 (2017)
   PUBMED   27694478
REFERENCE   7  (bases 1 to 5291)
  AUTHORS   Wang,X., Wang,H., Guo,B., Zhang,Y., Gong,Y., Zhang,C., Xu,H. and
            Wu,X.
  TITLE     Gen1 and Eme1 Play Redundant Roles in DNA Repair and Meiotic
            Recombination in Mice
  JOURNAL   DNA Cell Biol 35 (10), 585-590 (2016)
   PUBMED   27383418
  REMARK    GeneRIF: Study found that a Gen1 mutation alone did not affect DNA
            repair or meiotic recombination in mice; it caused synthetic
            lethality when combined with Eme1 mutations at an early embryonic
            stage. Also, combination of Gen1 and Eme1 mutations makes embryonic
            fibroblasts more sensitive to DNA damage & mice less capable of
            meiotic recombination. These results indicate that Gen1 and Eme1
            are functionally redundant in mice.
REFERENCE   8  (bases 1 to 5291)
  AUTHORS   Sun,L., Zhang,Y., Pan,Z., Li,B., Sun,M. and Zhang,X.
  TITLE     Expression and localization of GEN1 in mouse mammary epithelial
            cells
  JOURNAL   J Biochem Mol Toxicol 28 (10), 450-455 (2014)
   PUBMED   24980922
  REMARK    GeneRIF: The results suggest that GEN1 may play an important role
            in the development of mammary gland.
REFERENCE   9  (bases 1 to 5291)
  AUTHORS   Ding,S., Wu,X., Li,G., Han,M., Zhuang,Y. and Xu,T.
  TITLE     Efficient transposition of the piggyBac (PB) transposon in
            mammalian cells and mice
  JOURNAL   Cell 122 (3), 473-483 (2005)
   PUBMED   16096065
REFERENCE   10 (bases 1 to 5291)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC104880.13 and BC090653.1.
            
            On Feb 9, 2019 this sequence version replaced NM_177331.4.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK031077.1, BC090653.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164140 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                AC104880.13        190907-190926       c
            21-3303             BC090653.1         1-3283
            3304-5291           AC104880.13        164057-166044       c
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 5.58 cM"
     gene            1..5291
                     /gene="Gen1"
                     /gene_synonym="5830483C08Rik"
                     /note="GEN1, Holliday junction 5' flap endonuclease"
                     /db_xref="GeneID:209334"
                     /db_xref="MGI:MGI:2443149"
     exon            1..221
                     /gene="Gen1"
                     /gene_synonym="5830483C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            222..408
                     /gene="Gen1"
                     /gene_synonym="5830483C08Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             248..2974
                     /gene="Gen1"
                     /gene_synonym="5830483C08Rik"
                     /note="Gen homolog 1, endonuclease"
                     /codon_start=1
                     /product="flap endonuclease GEN homolog 1"
                     /protein_id="NP_796305.3"
                     /db_xref="CCDS:CCDS36403.1"
                     /db_xref="GeneID:209334"
                     /db_xref="MGI:MGI:2443149"
                     /translation="MGVNDLWQILEPVKQHIHLQDLSGKTIAVDLSLWVCEAQTVKKM
                     IGTVKKPHLRNLFFRISYLTQMNVKLVFVMEGEPPMLKADVISKRTQTRYGPSGKSRS
                     QKTGRSHFKSVLRECLEMLECLGMPWVQAAGEAEAMCAYLNASGHVDGCLTNDGDAFL
                     YGAQTVYRNFTMNTKDPHVDCYTISSIKSKLGLDRDALVGLAVLLGCDYLPKGVPGVG
                     KEQALKLLQIFKGQSLLQRFNQWIEDPCYSVPQSAPKKVVHCSVCSHPGSPKDHERNG
                     CILCKSDKYCEPHDYDYLCPCEWHQTDHNRHLSEIENNIKKKACSCEGFPFHEVIQEF
                     LLNKNKMLKPITYQRPDLLLFQRFTVQKMEWPSHYACEKLLVLLTRYDMIERKHGRKT
                     SNQLQPIRIVKPRVRNGVHCLEIEWEKPEHYVVEDGDPGKLSLLTMEEASLFEAAYPD
                     AVAVYQKQLSETKGRKQKSMKNKPKGSHLPEADDVINSQSLMTLKPTSKAFPKQNPKI
                     NLENSPDPILAQESTSPSLNSFVSPENAPCLNLQEQLVPSPRTLAIKQSKDVSHFLVS
                     ECSQPSSSSHDISVITDLQLSTIDWAGTSFSNSPAVQRNTFSQDLASESESSAILPDF
                     EQLSYESEQGTSDSEGSGRDLQQSNPEEQLLSGISALHLHDLPLKERIRIKSSCPQYN
                     VGADAGLESLPLKLKGSCIAYSSSDGSSNFSKDLTGVYLHKESRNSKVLDSRLQENCG
                     ANTSLPYSFSDKAVKTSSLQVGLPTAAIPHNPRVAVKTTKNLVMKNSVCLERDSSDED
                     NAPGSWKSKYTAPEMKHSSQKHSLVHVRDSTHNKLRNPKVESKETKLCNESFKTAEDE
                     ENGFSDLGRSPQSFRPCHDKDENSTASWENPLPLRQRLKLRFQNTQSGFYNT"
     exon            409..595
                     /gene="Gen1"
                     /gene_synonym="5830483C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            596..772
                     /gene="Gen1"
                     /gene_synonym="5830483C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            773..883
                     /gene="Gen1"
                     /gene_synonym="5830483C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            884..957
                     /gene="Gen1"
                     /gene_synonym="5830483C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1046
                     /gene="Gen1"
                     /gene_synonym="5830483C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1197
                     /gene="Gen1"
                     /gene_synonym="5830483C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1234
                     /gene="Gen1"
                     /gene_synonym="5830483C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1315
                     /gene="Gen1"
                     /gene_synonym="5830483C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..1446
                     /gene="Gen1"
                     /gene_synonym="5830483C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1447..1508
                     /gene="Gen1"
                     /gene_synonym="5830483C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1509..1652
                     /gene="Gen1"
                     /gene_synonym="5830483C08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1653..5291
                     /gene="Gen1"
                     /gene_synonym="5830483C08Rik"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggactaatg gagaaggcgc tggattcgaa attactgccg ggagcttagt gctagggaga
       61 gcagcgggag gctcccccag cccttgggtt tgtttgtttg tttgctttaa ctttgtttga
      121 aggaattcgg gaacttaggg gcggtggtag tggttaactc cgggccaggc tggtagatga
      181 cagaagtgtg ccttccacag ccgcgaagag ggggtgtctg gataacacca gataatcaac
      241 catcaggatg ggagtgaatg acttatggca aattctagag cctgtaaagc aacatatcca
      301 cttgcaagat cttagtggga aaaccattgc cgttgatttg agtctctggg tatgcgaggc
      361 acagacagtg aagaaaatga taggaactgt caagaagccc cacctcagga acctattttt
      421 tcgtatttca tatttaactc aaatgaatgt aaaactggtg tttgtaatgg aaggggagcc
      481 accaatgctg aaagctgatg tcataagcaa gaggactcag actcgttacg ggccttctgg
      541 aaaatcaagg tctcagaaaa cagggagatc acattttaag tcagtcttac gagagtgcct
      601 tgaaatgctc gagtgcctgg gaatgccctg ggtccaggcc gccggggaag ctgaggccat
      661 gtgtgcttac cttaatgcca gtggccatgt ggatggctgc ctcaccaacg acggcgatgc
      721 tttcctttat ggagcccaga ctgtttacag aaatttcacg atgaacacaa aggatcccca
      781 tgttgactgt tacacgatat catctatcaa gagtaagcta ggcttggatc gagacgctct
      841 ggttggactg gccgtacttc tgggttgtga ttaccttcca aagggagtgc ctggagttgg
      901 aaaggaacaa gcattaaaac ttttgcagat tttcaaaggt caaagtttgc ttcagaggtt
      961 taaccagtgg attgaagacc cttgctactc tgttccacaa tcagcaccaa aaaaagtggt
     1021 tcactgctct gtgtgttccc acccaggttc gccaaaggat catgaacgta atggatgcat
     1081 attatgtaaa agtgataaat actgtgaacc acatgattat gattacctct gcccttgtga
     1141 atggcatcag acagaccata ataggcatct aagtgaaata gaaaacaata ttaaaaagaa
     1201 agcttgcagt tgcgaaggat ttccattcca tgaggtcatc caagagttcc ttttgaataa
     1261 gaataaaatg ttgaaaccga tcacatacca aagacctgat ttattattgt ttcagagatt
     1321 tactgttcaa aaaatggagt ggcccagtca ctatgcatgt gaaaaattgt tggtgctttt
     1381 gacccgctat gacatgatag aaagaaaaca tggtagaaag acctccaatc aactacagcc
     1441 aattagaatt gttaaacccc gagtcagaaa tggagtccat tgtcttgaaa tagaatggga
     1501 aaagcctgaa cattatgtgg tggaagatgg agaccctgga aaactgagcc tccttacaat
     1561 ggaagaagcg tccctgtttg aagcagccta tcctgatgct gtggctgtct accagaaaca
     1621 gctgtcagag accaaaggaa ggaaacagaa aagtatgaaa aataagccta aaggaagcca
     1681 tttaccggaa gcagatgatg tgatcaattc tcagtcactt atgactttaa aacctacatc
     1741 taaagccttc cccaagcaaa atcccaaaat taatttggaa aattctccag atcctatttt
     1801 agcacaggaa tctacttccc cctcattgaa tagttttgtt tcccctgaaa atgctccctg
     1861 tttgaattta caagaacagt tagtgccatc tcccagaact ttggctataa agcaaagtaa
     1921 agatgtcagt cattttctag tttcagaatg tagtcagccc agctcttcct cccatgacat
     1981 ctctgtgatt actgatctgc agttgagtac cattgattgg gcgggtactt cttttagtaa
     2041 ttctccagca gttcaaagaa acactttctc tcaagactta gcatcagaat ctgagtcatc
     2101 ggccatcctt cctgactttg aacagctgtc atatgaatca gaacaaggga cctcagacag
     2161 tgaagggtcc ggtagagacc ttcagcagag caaccctgaa gagcagctcc tttctggcat
     2221 cagtgcttta catcttcatg atttgcctct aaaagaaaga atacgtatca aatcttcatg
     2281 ccctcagtat aatgtaggag cagatgctgg cctggaaagt ttgcccctaa aattgaaagg
     2341 ctcttgtatt gcttacagta gctctgatgg ctcatccaat ttttcaaagg atcttacagg
     2401 ggtatatctt cacaaagagt ccagaaactc taaagttcta gacagccgac tccaagaaaa
     2461 ctgcggggcg aatacttcct taccttattc tttcagtgac aaagcagtaa agacttccag
     2521 tcttcaagtt gggctgccaa ctgctgctat acctcataat ccaagagttg ctgtgaaaac
     2581 tactaagaac cttgtcatga agaatagtgt ttgcctcgag agagattcct cagatgaaga
     2641 caatgctcca gggtcctgga aatcaaagta tactgctcca gaaatgaaac acagttctca
     2701 gaagcacagc ctagtccatg tcagagacag tactcacaac aaactcagaa atcctaaagt
     2761 ggaatctaaa gaaaccaaac tgtgtaatga gtcttttaaa acagctgaag atgaagaaaa
     2821 cgggttctca gatctaggga gaagtcctca gagttttcga ccatgtcatg acaaagatga
     2881 gaactctact gcctcctggg aaaatcctct acctttacgc cagaggttaa aactcaggtt
     2941 ccagaacact caaagtggat tttataatac ttaagtttaa tgtgtttgaa tgctgatagt
     3001 attagcagga gccaagcaaa ctgatgtttg gtagaaaatg tacatgagta tctgctgttt
     3061 aaatcaaaat tgtagtttta aaatgttaac agccaagtat ggctaatgcc tataatcaca
     3121 gcatgtaaga gggccatgga gtccagagga tcatccacca ggagtgcaga atcattctct
     3181 ggtatggaga gtgtcaggcc aatctcagtt atctaagaaa gaaaaacagt taaaagtttg
     3241 actataaaag atgagcatct ggtgacagct tcaaataata tatttttaga acattttatt
     3301 attcttgaaa tgtctttatt attatctagc ttttttttgt ttggttggtt tggtttggtt
     3361 ttggttttgg ttttttgaaa cagggtttct ctgtatagcc ctggctgtcc tggaactcac
     3421 tttgtagacc aggctggcct tgaactcaga aatctgcctg gctctgcctc ccgagtgctg
     3481 ggattaaagg cgtgcaccac cacgcccggc cttattatta tctagcttta acatatcggt
     3541 ttattctttg actgagtaaa tgctttatca tttttagttc ttgtatgtct aaagcatttg
     3601 tttatatatt atgttttatc cccaagatgg caacacttta taatagtgtt gcagtatctt
     3661 ttgtgggccc ttcctaatgg ctgtatttcc ccttcatgtt aaagaaccgt tagcatgctc
     3721 ttccactttt agtaatacca tgcttgagaa gtgaaaatgg aagcggatga tgtgtggtca
     3781 agggtggttg tgaactctgg ataaggaaag tcactcctta tatacattca gaagttcacg
     3841 ggcactgcaa gcaggaaata ttttaaaaat cagatgtaaa atcaaaaggg aattggaagc
     3901 ttccttggga atcgcacagt aataatagta atggtgtgga tcccatcttt ctggtttggt
     3961 tcttgagctt ttatttttgt tttgtttgta atctatagaa ttgttctctt ttgctttaaa
     4021 ttttcatttg taggtaatgt tgactttaat tcctacaaac cttaaatctt atataaatta
     4081 taaaatgcct aaaggcatct agggtttaaa agatatagaa cagctgtttg tgaatgactg
     4141 gatcctgaaa gtagaagatc aaaggaatcc atcaaaaact tattaaaagc aagccaggcg
     4201 tggcaacaca cacctttaat cctagcattt ggaaggcaaa ggcaagtgga tctctgaatt
     4261 caaggctggc ctggtagata gagctaattc caggacagcc agggctacac ataattcccg
     4321 tcttaaaaaa aaatcttttt aaacaaaaac aaaaactatc aaaagtgaag agtaagctag
     4381 caaggttata aaattcaaaa ttagtttcta catagttgta gttctgtata atcagtgtat
     4441 gacaatgaaa tttcaccaag tgaattaaag tactctgttt acaacatcaa aaaaaaaaaa
     4501 aaaaaaaaaa agccgggcag tggtggcgca cgcctttaat cccagcactt gggaggcaga
     4561 ggcaggtgga tttctgagtt cgaggccagc ctggtctaca aagtgagttc caggacagcc
     4621 cgggctacac agagaaaccc tgtctcgaaa caaaacaaaa caaaacaaaa caaaacaaac
     4681 aaacaaaaaa catagcaaaa cttagttacc agtgaaaaac aatggatgaa aataaagtat
     4741 aaataaaaag aggcatccca gattcatgga tcagaagact taacttggta gtactgttaa
     4801 actggcgagt ttaactacca gcacttggaa ggtagaggta ggcgaattgc ttgtgttaga
     4861 ggccagcctg gtctacagat caagttccag aacagccaag gctatacaga aaaaccctat
     4921 ctcaaaaaac taaccaatca agagaaaaat agacaactgt ttggtttcaa tccagtccct
     4981 tcaatatcat gtttgtttgc ttccataccc ccacccccag aacatgacaa gctgatttca
     5041 ttgttcagat agaaatttaa caaaataaca catcttggga aaatcggaga attcacactt
     5101 ccttattcct gattactaca aggttgtata aattaaatat gttatactga tataagggta
     5161 gatatgtaga taagtgagtt ggacagaatc tagtctagaa atgtaccctc atatttatgg
     5221 tcaattgcat ttttagccag tatgaagaca gcaatgggaa aacaagtctc aataaatgct
     5281 tctggaactt t
//
