LOCUS       NM_146238               1862 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus gem nuclear organelle associated protein 8 (Gemin8),
            transcript variant 2, mRNA.
ACCESSION   NM_146238
VERSION     NM_146238.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Carissimi,C., Saieva,L., Gabanella,F. and Pellizzoni,L.
  TITLE     Gemin8 is required for the architecture and function of the
            survival motor neuron complex
  JOURNAL   J Biol Chem 281 (48), 37009-37016 (2006)
   PUBMED   17023415
  REMARK    GeneRIF: Gemin8 has an essential role in the proper structural
            organization of the SMN complex and the involvement of the
            heteromeric subunit containing Gemin6, Gemin7, Gemin8, and Unrip in
            the recruitment of Sm proteins to the snRNP assembly pathway
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Carissimi,C., Saieva,L., Baccon,J., Chiarella,P., Maiolica,A.,
            Sawyer,A., Rappsilber,J. and Pellizzoni,L.
  TITLE     Gemin8 is a novel component of the survival motor neuron complex
            and functions in small nuclear ribonucleoprotein assembly
  JOURNAL   J Biol Chem 281 (12), 8126-8134 (2006)
   PUBMED   16434402
  REMARK    GeneRIF: Gemin8 is a novel integral component of the SMN complex
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL713990.16, AK160583.1 and AK053774.1.
            
            On Jul 18, 2015 this sequence version replaced NM_146238.3.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants 1 and 2 encode the same protein
            (isoform 1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK053774.1, AK160583.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                AL713990.16        71799-71824
            27-63               AK160583.1         2-38
            64-1862             AK053774.1         2-1800
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 76.75 cM"
     gene            1..1862
                     /gene="Gemin8"
                     /gene_synonym="B130034M20Rik; B930082C09Rik; gemin-8"
                     /note="gem nuclear organelle associated protein 8"
                     /db_xref="GeneID:237221"
                     /db_xref="MGI:MGI:2384300"
     exon            1..97
                     /gene="Gemin8"
                     /gene_synonym="B130034M20Rik; B930082C09Rik; gemin-8"
                     /inference="alignment:Splign:2.1.0"
     exon            98..136
                     /gene="Gemin8"
                     /gene_synonym="B130034M20Rik; B930082C09Rik; gemin-8"
                     /inference="alignment:Splign:2.1.0"
     CDS             134..850
                     /gene="Gemin8"
                     /gene_synonym="B130034M20Rik; B930082C09Rik; gemin-8"
                     /note="isoform 1 is encoded by transcript variant 2;
                     gem-associated protein 8"
                     /codon_start=1
                     /product="gem-associated protein 8 isoform 1"
                     /protein_id="NP_666350.2"
                     /db_xref="CCDS:CCDS41207.1"
                     /db_xref="GeneID:237221"
                     /db_xref="MGI:MGI:2384300"
                     /translation="MASNWRASASWYSHPVYARYWQHYHHAMLWMQGHQNAYRKFRDS
                     YFTSPWLFPHGALPWNSPAYEAGHPWDSQGQHMAQQESPYRVSHPKSPGQPLRNSSRT
                     QASTRGNEARCEEEELESDSDDEVECDLSNMEITEELRQYFAQTERHREERRRQQQLD
                     AERLNDYVNADHGLYFNHRRSLEPPSEKPWERRQAEMKRLYGNSAPKILAMETAVQLS
                     FDKHCDRKQPKYWPVIPLKF"
     exon            137..593
                     /gene="Gemin8"
                     /gene_synonym="B130034M20Rik; B930082C09Rik; gemin-8"
                     /inference="alignment:Splign:2.1.0"
     exon            594..1862
                     /gene="Gemin8"
                     /gene_synonym="B130034M20Rik; B930082C09Rik; gemin-8"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1094
                     /gene="Gemin8"
                     /gene_synonym="B130034M20Rik; B930082C09Rik; gemin-8"
                     /note="major polyA site"
     regulatory      1850..1855
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gemin8"
                     /gene_synonym="B130034M20Rik; B930082C09Rik; gemin-8"
                     /note="hexamer: AATAAA"
     polyA_site      1861
                     /gene="Gemin8"
                     /gene_synonym="B130034M20Rik; B930082C09Rik; gemin-8"
                     /note="major polyA site"
ORIGIN      
        1 cactccccca cttctcccgc cccttcctcc tccccaccac cagagctagg tccgttgttg
       61 cccaccgccc gagatctgcc agctgcgtgc ccacgagttc ccacttggac ttttgcaatc
      121 agagataaca gcaatggcct ccaactggag ggctagtgca tcttggtatt ctcacccagt
      181 atatgcaaga tactggcaac attatcatca cgcaatgctt tggatgcagg gccaccagaa
      241 tgcctacaga aagttcaggg attcctattt cacatccccg tggctcttcc ctcatggagc
      301 tcttccctgg aactctcctg cttatgaggc tgggcatcct tgggactctc aaggccagca
      361 catggcgcag caggagtctc cctaccgtgt ttcccatccc aaaagccctg ggcagcctct
      421 gcgtaatagc agtagaaccc aggcatccac aagagggaat gaagcacggt gtgaggagga
      481 agagctggag tcagattcag atgatgaagt agagtgcgac ctgagcaata tggagatcac
      541 cgaggagctc cggcagtact tcgcgcagac cgagaggcac agagaggaga gaaggcgaca
      601 gcagcagctg gacgcagagc gcctgaatga ctatgtgaat gcggaccatg gcctgtactt
      661 caaccaccgt aggtcactgg aacccccatc tgagaagccc tgggagcggc gccaagcaga
      721 gatgaagcgc ttatatggca acagtgctcc caagatcctg gccatggaga ctgctgtaca
      781 gctgagcttt gacaagcact gtgacagaaa gcaaccaaag tactggcctg tcattcccct
      841 gaagttctga gtcctgggca ccgggaccct agggaatgca tcacagttcc tagcctctcc
      901 ctcttcatct cttctggaca tttttcagag aaagaggcct gtataccaac atggtggata
      961 cagtctgtca acaggagaga ggtatactgt tgccaacaca aggaatgttc actgacaaag
     1021 tgataattta tgtattgtac tggctcttgg ttttctcttt aacagtccag ttcattgaaa
     1081 ttattgccta gcaatactgc ctattgtttt gcatttttaa tgtccttctt taaaccatcc
     1141 agaatgtcat aaaagcctta agccacgtgt catttattcc tacattttta ttagatattt
     1201 tctttattta tattttaaat gttatcccct ttccttgttt cccctccttt aaaagctccc
     1261 tatcccctgc tccccagccc acccactcca catcctggcc cagacattcc tacattttta
     1321 ggccagtttt catacagtgc atgctagcta caaacttaaa atcttcctgc ttccacctcc
     1381 caaatgctcg tttgcaggtt tgcactgccc acactgagct tctgtccacg tattttctgt
     1441 gactttttct gtggacccct ctgaaaacca tgtaagtgaa cagtaaaaga agtggcactg
     1501 tgcaaggtgt ggtagcgcac gcctttaatc ccagcactcc ggaggcagag gcaggaggat
     1561 ttctgagttc gaggccagcc tggtctacaa agtaagttcc aggacagcca gggctataca
     1621 gagaaaccct gtcttggggg gaaaaaaaag tggcacttgt tattctgcta ggtgtcagcc
     1681 cgcatgctct ataagacctg agagtaagta ctctgggctt ggcaggccat gttgtctcat
     1741 aactgcctaa ccctgtcttt atagcataaa aacagccata gttactgaat aaacaaatga
     1801 atgtgactgt gtttcaataa aattttattt gtaaaaaaaa ataaaataaa ataaaacagg
     1861 ct
//
