LOCUS       NM_172558               6226 bp    mRNA    linear   ROD 08-OCT-2024
DEFINITION  Mus musculus gem nuclear organelle associated protein 5 (Gemin5),
            transcript variant 2, mRNA.
ACCESSION   NM_172558
VERSION     NM_172558.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Salman,B., Bon,E., Delers,P., Cottin,S., Pasho,E., Ciura,S.,
            Sapaly,D. and Lefebvre,S.
  TITLE     Understanding the Role of the SMN Complex Component GEMIN5 and Its
            Functional Relationship with Demethylase KDM6B in the
            Flunarizine-Mediated Neuroprotection of Motor Neuron Disease Spinal
            Muscular Atrophy
  JOURNAL   Int J Mol Sci 25 (18), 10039 (2024)
   PUBMED   39337533
  REMARK    GeneRIF: Understanding the Role of the SMN Complex Component GEMIN5
            and Its Functional Relationship with Demethylase KDM6B in the
            Flunarizine-Mediated Neuroprotection of Motor Neuron Disease Spinal
            Muscular Atrophy.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6226)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 6226)
  AUTHORS   Takada,Y., Fierro,L., Sato,K., Sanada,T., Ishii,A., Yamamoto,T. and
            Kotani,T.
  TITLE     Mature mRNA processing that deletes 3' end sequences directs
            translational activation and embryonic development
  JOURNAL   Sci Adv 9 (47), eadg6532 (2023)
   PUBMED   38000026
REFERENCE   4  (bases 1 to 6226)
  AUTHORS   Bizen,N., Bepari,A.K., Zhou,L., Abe,M., Sakimura,K., Ono,K. and
            Takebayashi,H.
  TITLE     Ddx20, an Olig2 binding factor, governs the survival of neural and
            oligodendrocyte progenitor cells via proper Mdm2 splicing and p53
            suppression
  JOURNAL   Cell Death Differ 29 (5), 1028-1041 (2022)
   PUBMED   34974536
REFERENCE   5  (bases 1 to 6226)
  AUTHORS   Rajan,D.S., Kour,S., Fortuna,T.R., Cousin,M.A., Barnett,S.S.,
            Niu,Z., Babovic-Vuksanovic,D., Klee,E.W., Kirmse,B., Innes,M.,
            Rydning,S.L., Selmer,K.K., Vigeland,M.D., Erichsen,A.K.,
            Nemeth,A.H., Millan,F., DeVile,C., Fawcett,K., Legendre,A.,
            Sims,D., Schnekenberg,R.P., Burglen,L., Mercier,S., Bakhtiari,S.,
            Francisco-Velilla,R., Embarc-Buh,A., Martinez-Salas,E., Wigby,K.,
            Lenberg,J., Friedman,J.R., Kruer,M.C. and Pandey,U.B.
  TITLE     Autosomal Recessive Cerebellar Atrophy and Spastic Ataxia in
            Patients With Pathogenic Biallelic Variants in GEMIN5
  JOURNAL   Front Cell Dev Biol 10, 783762 (2022)
   PUBMED   35295849
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6226)
  AUTHORS   Workman,E., Kalda,C., Patel,A. and Battle,D.J.
  TITLE     Gemin5 Binds to the Survival Motor Neuron mRNA to Regulate SMN
            Expression
  JOURNAL   J Biol Chem 290 (25), 15662-15669 (2015)
   PUBMED   25911097
  REMARK    GeneRIF: This work both reveals a new autoregulatory pathway
            governing SMN expression, and identifies a new mechanism through
            which SMN can modulate specific mRNA expression via Gemin5.
REFERENCE   7  (bases 1 to 6226)
  AUTHORS   Cobellis,G., Nicolaus,G., Iovino,M., Romito,A., Marra,E.,
            Barbarisi,M., Sardiello,M., Di Giorgio,F.P., Iovino,N., Zollo,M.,
            Ballabio,A. and Cortese,R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 6226)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Kitamura,H., Nakagawa,T., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of FLJ
            genes: the complete nucleotide sequences of 110 mouse
            FLJ-homologous cDnas identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (2), 127-135 (2004)
   PUBMED   15449545
REFERENCE   9  (bases 1 to 6226)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 6226)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY742908.1, AK143536.1, AK162955.1 and AI462876.1.
            
            On Oct 31, 2009 this sequence version replaced NM_172558.2.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice junction at the 5' end of an exon compared to variant 1. The
            resulting isoform (2) has the same N- and C-termini but is 1 aa
            shorter compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK143536.1, AK049216.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-52                BY742908.1         2-53
            53-4922             AK143536.1         2-4871
            4923-6209           AK162955.1         2895-4181
            6210-6226           AI462876.1         1-17                c
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 35.72 cM"
     gene            1..6226
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /note="gem nuclear organelle associated protein 5"
                     /db_xref="GeneID:216766"
                     /db_xref="MGI:MGI:2449311"
     exon            1..252
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..4595
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /note="isoform 2 is encoded by transcript variant 2;
                     gem-associated protein 5"
                     /codon_start=1
                     /product="gem-associated protein 5 isoform 2"
                     /protein_id="NP_766146.2"
                     /db_xref="CCDS:CCDS24723.1"
                     /db_xref="GeneID:216766"
                     /db_xref="MGI:MGI:2449311"
                     /translation="MKPEPRTLPPSPNWYCSRCSDAAPGGIFGFAARTSVFLVRVGPG
                     AGASPGAPPFRVVGELVGHTERVSGFTFSHHPGQYNLCATSSDDGTVKVWDVETKTVV
                     TEHTLHQHTISALHWSPTVKDLIVSGDEKGVVFCYWLNRNDSQHLFTEPRTIFCLTCS
                     PHHENLVAIGYKDGIVVIIDISKKGEVIHRLRGHDDEIHSIAWCPLSGEDCLSISQEE
                     NSEEPDIPNGKLIAETPITKGCYLATGSKDQTIRIWSCSRGRGVMVLKLPFLKRRSGG
                     VDPTVKERLWLTLHWPKNQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSTSAEGHNHS
                     RIVFNLCSLKTEDGKQLLLSTSMDRDVKCWDMATLECCWTLPSLGGFAYSLAFSPVDV
                     GSLAIGVGDGMIRVWNTLSIKNNYDVKNFWQGVKSKVTALCWHPNKEGCLAFGTDDGK
                     VGLYDTCSNKPPQISSTYHKKTVYRLAWGPPVPPMSLGGEGDRPSLTLYSCGGEGVVL
                     QHNPWKLSGEAFDINKLVRDTNSIRYKLPVHTEISWKGDGKVLALGNEDGSIEIFQVP
                     NLRLLCTIQQHHKLVNAIVWHHEHGSRPELSCLLASGSNNAVIYVHNLKAVLESNPES
                     PITITEPYRTLSGHTAKITSLAWSPHHDGRLVSACYDGTAQVWDALREEPLFNFRGHR
                     GRLLCVAWSPVDPECIYSGADDFCVYRWLTSMQDHSRPPQGKKCIELEKKRLSQFKPK
                     LKKKKKPTLRLPVKQDSSVGNEDESVKENSGPAENGLSDQDGEEEAQEPELPPSPVVC
                     VEPVSCTDICSGFEKSKVTVSSKATSLKKEPAKEKPEALLKKRKARSMLPLSTSLDHR
                     SKEELHRDCLVLATATHAKAELNEDVSADLEERFHLGLFTDRATLYRMMETEGKGHLE
                     SGHPELFHQLMLWKGDLKGVLQAAAERGELTDSLVAVAPVAGYSVWLWAVEAFAKQLC
                     FQDQYVKAASYLLSIHKVYEAVELLKSNHLYREAIAVAKARLRPEDPVLKELYLSWGS
                     ILERDGHYAIAAKCYLGATSAYDAAKVLARKGDAASLRTAAELAAIAGEHELAASLAL
                     RCAQELLLMKNWVGAQEALGLHESLQGQRLVFCLLELLCRHLEEKQPLEVRGPSSIYH
                     QWATGSEGTLVQRVTGVWRSAFSVDTPEQCQAALQKLQDVKYPSATSNTPFRQLLLHV
                     CHDLTLAMLSQQAAAWEEAVPALLQAVVRSYTSGNFTLMQEIYSAFLPGGCDHLRDKL
                     GDLSPAMAAYKSLEAFCIYGQLYEVWWSLCGPGPESSVWVLSAESTVSDKQSKPEDSA
                     SAEDMEQPPGPGPRLSAESERLLSACKELFSERHASLQTSQRTVAEVQETLAEMIRQH
                     QKSQLCKATTNGPSRDEPSRDEPSQEAERAPSQPPSPTEERNAPVSLPELTRRLTEAN
                     ERIAEFPESVKAWPFPDVLECCLVLLHIGSQCPDAVDPEMQQQAQELLHKYGHTRAYR
                     RHCQSRHT"
     exon            253..413
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            414..595
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            596..747
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            748..867
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            868..1000
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1166
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1379
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1465
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            1466..1548
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..1685
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            1686..1759
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            1760..1941
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            1942..2081
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            2082..2253
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            2254..2475
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            2476..2589
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            2590..2712
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            2713..2811
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            2812..2949
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            2950..3097
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            3098..3217
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            3218..3428
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            3429..3683
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            3684..3846
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            3847..4333
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            4334..4427
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     exon            4428..6211
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4906..4911
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /note="hexamer: TATAAA"
     polyA_site      4923
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /note="major polyA site"
     regulatory      5178..5183
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /note="hexamer: AATAAA"
     polyA_site      5207
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
     regulatory      6185..6190
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
                     /note="hexamer: ATTAAA"
     polyA_site      6211
                     /gene="Gemin5"
                     /gene_synonym="C330013N08"
ORIGIN      
        1 aaatcgatcc cgccccctcc cgctccacgc cgcggttgcg aaccaccgtc ccagagctcc
       61 tctgctgagc ttgggcggcc gcggagatga agccggagcc gcggacgctg ccgccgtccc
      121 ccaactggta ctgctcacgc tgcagcgacg cggcgcccgg aggcatcttt ggcttcgctg
      181 cgcggacctc cgtcttcctc gtccgcgtgg gtccgggtgc gggcgcgagc ccaggggcgc
      241 ccccgtttcg agtggtagga gagttggtgg gacacactga aagagtatct ggtttcactt
      301 tttctcatca ccctggacaa tacaacctct gtgccaccag ctcagacgat ggaactgtga
      361 aggtttggga tgtagagacc aagactgtag tgacagaaca cacactccat cagcatacaa
      421 tatcagcact gcattggtct ccgacagtta aggacttaat agtctctggg gatgaaaaag
      481 gagtcgtttt ctgttactgg cttaacagaa atgacagcca gcacctcttc acagagccca
      541 ggaccatatt ctgtctgacg tgttcacctc atcacgaaaa tctagtagcc attggctaca
      601 aagatggcat agtggttatc attgacatca gtaaaaaggg agaagttatt cacagacttc
      661 gaggccacga tgacgagatc cactccatag cctggtgtcc tctgtctggt gaagactgct
      721 tatccatcag ccaagaggaa aactcggaag aacctgacat tcccaatggg aagcttatag
      781 cagaaacccc catcacaaaa ggctgctact tagccacagg aagcaaagat cagaccattc
      841 gaatctggag ctgttcccgc ggccggggtg taatggtttt gaaattgccc ttcctgaaga
      901 gaagaagtgg gggtgtggac ccaacagtta aagaacggct ttggttgaca cttcattggc
      961 ctaagaacca accaacacag ctggtatcca gttgttttgg aggtgagctg ctgctatggg
     1021 acctcaccca gtcatggagg cggaagtaca cgctcttcag cacctcagca gaagggcaca
     1081 atcattccag aattgtcttc aatctgtgtt ctctgaagac tgaagatggc aagcagctgc
     1141 tactgtccac atccatggat agagatgtaa aatgttggga catggccacc ctggagtgtt
     1201 gctggacatt gccttctctt ggaggttttg cctacagcct ggccttttct cctgtggacg
     1261 tgggctccct ggccataggt gttggggacg gcatgatccg ggtgtggaat acactctcca
     1321 taaagaacaa ctacgacgtg aaaaacttct ggcaaggagt caagtccaag gtcacagcgc
     1381 tatgctggca cccaaacaag gaaggctgct tagccttcgg gaccgatgac ggaaaagtgg
     1441 gattgtatga cacctgctcc aacaaacctc cacagatttc aagcacgtat cataagaaga
     1501 ccgtgtatag gttggcttgg gggccaccag tcccccccat gtcacttgga ggggaaggag
     1561 acaggccttc cctcaccttg tacagctgtg gaggggaagg cgttgtgcta cagcataacc
     1621 cctggaagct cagtggagag gccttcgaca tcaacaaact cgtgagggac accaactcca
     1681 ttagatacaa actgcccgtg cacacagaga tcagctggaa aggagatggc aaagtcctgg
     1741 ctcttgggaa tgaagatgga tcaatagaaa tattccaagt ccccaacttg aggctgctgt
     1801 gcaccatcca gcagcaccac aagcttgtga acgccatagt ctggcaccat gagcatggca
     1861 gccgtccaga gctgagctgt ctccttgctt ctggctccaa caatgcagtc atctacgtgc
     1921 acaacctgaa agcagtccta gaaagcaatc ctgagtctcc aataaccatc acagagccct
     1981 accgcaccct ctcggggcac acagccaaga ttaccagtct ggcatggagc ccacatcatg
     2041 acggaaggct ggtatctgct tgctacgatg gcacagctca ggtgtgggat gccctccggg
     2101 aagaaccttt gttcaatttc cgaggacatc gaggccggct gctatgtgtc gcgtggtccc
     2161 cagtggatcc agaatgcatc tactcaggtg cagatgactt ctgtgtctac aggtggctga
     2221 cttccatgca ggaccattcc cggcctcctc aagggaaaaa atgtattgaa ctagagaaaa
     2281 aacgactctc tcaatttaag ccaaagctta aaaagaagaa aaaacctacc ttgcgactgc
     2341 ctgtaaagca ggattcatcc gttggcaatg aagatgagag tgtaaaggag aactctggac
     2401 ctgctgagaa cggcttgtcg gaccaggatg gtgaggagga agcccaggag ccagagctgc
     2461 ctccctctcc tgtagtttgt gtagaacctg tttcctgtac tgacatttgc tcaggctttg
     2521 aaaagtcaaa agtcactgtt agtagcaaag ccacctcact gaaaaaggag ccagctaaag
     2581 agaaaccaga agccttgctc aagaagagga aggctcgctc catgctccct ctgagcacca
     2641 gcttagacca caggtccaaa gaggagctcc atcgagactg cttggtacta gcaactgcaa
     2701 cacacgccaa agcagagcta aatgaagatg tgtctgccga ccttgaggaa cgattccact
     2761 tgggcctttt cacagacagg gctactctat acaggatgat ggaaacagaa gggaaaggtc
     2821 acttggaaag tggccacccc gagctttttc accagctcat gctctggaaa ggagatctga
     2881 agggtgttct ccaggctgca gcagagagag gcgagctgac agacagtctc gtggctgtgg
     2941 caccagtggc tggctacagt gtatggctgt gggctgtgga agcctttgcc aagcagctgt
     3001 gtttccagga ccagtacgtc aaggctgcct cttatctgct atccatccac aaagtgtacg
     3061 aagctgtgga acttctcaag tcaaaccatc tctacagaga agctattgca gttgccaagg
     3121 ctcggctgcg ccctgaggac cctgttctga aggaactata cctcagctgg gggtccatcc
     3181 tggagagaga tggccactat gctattgcag ccaagtgcta cttaggagcc acttccgcct
     3241 atgatgcagc caaagttctg gccagaaagg gagatgcagc ctcacttaga acagctgcag
     3301 agctggctgc catagcagga gaacatgagc tggctgcttc cctggctctt agatgtgccc
     3361 aagagctgct tctgatgaag aactgggtgg gtgcacaaga agcattggga ctgcatgaaa
     3421 gcctgcaggg ccagagactg gtcttctgcc tccttgaact tctatgccgg cacctggagg
     3481 aaaaacagcc tctagaggtc agaggcccct cttccattta ccaccagtgg gccacaggtt
     3541 ctgagggaac cttggtgcag agagtaaccg gtgtgtggcg cagcgccttc agtgtggaca
     3601 ccccggagca gtgtcaggcc gccttacaga agctgcagga tgtcaagtac ccatcagcaa
     3661 caagtaacac tcccttcaga cagctgctgc ttcatgtctg ccatgacctg accttggcaa
     3721 tgctgagcca gcaggctgct gcctgggagg aggcagtgcc tgctctgctg caggctgtgg
     3781 ttcggagcta tacctcaggg aacttcaccc tcatgcagga aatctactca gcctttctcc
     3841 caggtggctg tgaccaccta cgagacaaac tgggtgacct ctctcctgcc atggcagctt
     3901 acaaaagctt agaggccttt tgtatttatg ggcaactgta tgaagtctgg tggtcgctct
     3961 gcgggcctgg ccctgagtca agcgtctggg tgttgtcagc tgaaagcacg gtctctgata
     4021 aacagagcaa gccagaggac agtgccagtg ctgaggacat ggaacagcct ccaggcccgg
     4081 gcccgaggct cagtgcagag agtgagcgtt tgctgagtgc ctgcaaggag cttttctcag
     4141 aacggcatgc cagcctgcag acctcccaga ggactgtagc cgaagtccaa gagacactgg
     4201 cagaaatgat ccgccagcac caaaagagtc agctctgcaa ggccacaaca aatggtccca
     4261 gcagagatga acccagcaga gatgaaccca gccaggaagc agagcgggct ccttctcagc
     4321 ctccgagccc aacagaagaa agaaatgcac cagtgtctct ccctgaacta accagaaggc
     4381 tcacagaggc aaatgagagg atagcagagt ttccagagag tgtaaaggcc tggccctttc
     4441 cagatgtgct ggagtgctgt ctcgtcttgc ttcacattgg gtcccagtgt cctgacgctg
     4501 tggaccctga gatgcagcaa caggcccagg agctccttca caagtatggg cacactcgag
     4561 cttacagaag acactgccag agtcggcaca cgtgaacttc cagaggccat aaagctgtgc
     4621 cacaccaccc acgacagctt cacctcggtc gtggctcacc aggccttcac cctgaccttc
     4681 aacggaagga tgctgtgatc caactgacag atgagggtga agtttgaatc cattcacaat
     4741 ggagcttgcc tggtacctct caagtggctt cctgtagtgg agggacaagc cagcagggtc
     4801 actaagaact aaccttgcca agactgtctc aagccttatg tggtttcccc aaagagaagc
     4861 ctttgtattc acccaaagtc tgtccacccc agatgcagct gcctttataa atcctcacca
     4921 tcagaaatgt ctcgttctgt gggaggtata ctcgacacat acctatcact gttggagttt
     4981 ggggttctta attatattgt tagctggacc tgcccacctc aaagactaaa ggaattagcc
     5041 ttggaacatt aggttatttt attttttacg gagttggtta catggacaag tagcaaaagc
     5101 tttcccagaa cagagctagt gaggctggct ttttcctcca ggttgtgtga ctggcagtgg
     5161 tcagacagct cagggaaaat aaaaatcact cttagtggtt catgtaatct tttggggtca
     5221 tagaatcctt ttttgagaat tgacttaaca cagcctagag aacaatgtgt cttccttttg
     5281 tgtatgcctc ccagaacagt aggctcctac cttacaccct gcagccccaa gtactatcta
     5341 ttcaccagtc ctgggctggc tgccagagtt accacccagc aaggaaggtc tgagacctgt
     5401 gcctccattg tcactggttt ctggaacagg agaagcgggt gtgctcaggc cgggtgggaa
     5461 gagcatgcct tcctcctctt tctccctaag gtccattgta aaggcttcat ctttgggcag
     5521 caagagattt tcccaactgg aaatcctcgg gacatacatc ttgagatact tgtatatatc
     5581 tagctttggg atttaaaaaa tgctaagtcc cccaaaagaa aatactgtcc agatgagggt
     5641 gaaacttgaa tccactcaca caatagagcc tgcctggtgc ctcccaagtg gcttcctgtg
     5701 atggagggaa aagccagcag ggtcactaga cccctgccaa gactgtctca agccttacat
     5761 gtgaactcaa ggaagatgca ttggtctgaa aggcaagagt cttgacaaag ctgtgagtgg
     5821 gcatgactat atggaagact ggatagctcc aagtagcttc atctatagag atggggaacg
     5881 ttggttccct ccacttaaag agcattggta aactacaaac tcatctttgt ggtttgctga
     5941 aacaagtacc tgttgggagt aatcctgcac actgcatgcc tctttcttct gaggaagact
     6001 cagaggcagg agggattcaa attcaagtcc agcctaggca gtgaagcgag atcttccctc
     6061 aaaatagtca ataatctaag agactagaga gagggcttgg gttaagagca ctggttgcat
     6121 ttccacagga tctgggtggt tttaacccca caagaaacct aaagtaaaac tatccaatag
     6181 gagaattaaa atggcaactg ttttcttctt aaaaaaaaaa aaaaaa
//
