LOCUS       NM_025656               1590 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus gem nuclear organelle associated protein 2 (Gemin2),
            mRNA.
ACCESSION   NM_025656
VERSION     NM_025656.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Bizen,N., Bepari,A.K., Zhou,L., Abe,M., Sakimura,K., Ono,K. and
            Takebayashi,H.
  TITLE     Ddx20, an Olig2 binding factor, governs the survival of neural and
            oligodendrocyte progenitor cells via proper Mdm2 splicing and p53
            suppression
  JOURNAL   Cell Death Differ 29 (5), 1028-1041 (2022)
   PUBMED   34974536
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Fallini,C., Rouanet,J.P., Donlin-Asp,P.G., Guo,P., Zhang,H.,
            Singer,R.H., Rossoll,W. and Bassell,G.J.
  TITLE     Dynamics of survival of motor neuron (SMN) protein interaction with
            the mRNA-binding protein IMP1 facilitates its trafficking into
            motor neuron axons
  JOURNAL   Dev Neurobiol 74 (3), 319-332 (2014)
   PUBMED   23897586
REFERENCE   3  (bases 1 to 1590)
  AUTHORS   Tang,F., Barbacioru,C., Nordman,E., Bao,S., Lee,C., Wang,X.,
            Tuch,B.B., Heard,E., Lao,K. and Surani,M.A.
  TITLE     Deterministic and stochastic allele specific gene expression in
            single mouse blastomeres
  JOURNAL   PLoS One 6 (6), e21208 (2011)
   PUBMED   21731673
REFERENCE   4  (bases 1 to 1590)
  AUTHORS   Todd,A.G., Morse,R., Shaw,D.J., McGinley,S., Stebbings,H. and
            Young,P.J.
  TITLE     SMN, Gemin2 and Gemin3 associate with beta-actin mRNA in the
            cytoplasm of neuronal cells in vitro
  JOURNAL   J Mol Biol 401 (5), 681-689 (2010)
   PUBMED   20620147
  REMARK    GeneRIF: utilised a targeted screen to identify mRNA associated
            with SMN, Gemin2 and Gemin3 in the cytoplasm of a human
            neuroblastoma cell line, SHSY5Y
REFERENCE   5  (bases 1 to 1590)
  AUTHORS   Shaw,L., Johnson,P.A. and Kimber,S.J.
  TITLE     Gene expression profiling of the developing mouse kidney and embryo
  JOURNAL   In Vitro Cell Dev Biol Anim 46 (2), 155-165 (2010)
   PUBMED   19998061
REFERENCE   6  (bases 1 to 1590)
  AUTHORS   Gabanella,F., Carissimi,C., Usiello,A. and Pellizzoni,L.
  TITLE     The activity of the spinal muscular atrophy protein is regulated
            during development and cellular differentiation
  JOURNAL   Hum Mol Genet 14 (23), 3629-3642 (2005)
   PUBMED   16236758
REFERENCE   7  (bases 1 to 1590)
  AUTHORS   McKee,A.E., Minet,E., Stern,C., Riahi,S., Stiles,C.D. and
            Silver,P.A.
  TITLE     A genome-wide in situ hybridization map of RNA-binding proteins
            reveals anatomically restricted expression in the developing mouse
            brain
  JOURNAL   BMC Dev Biol 5, 14 (2005)
   PUBMED   16033648
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 1590)
  AUTHORS   Jablonka,S., Holtmann,B., Meister,G., Bandilla,M., Rossoll,W.,
            Fischer,U. and Sendtner,M.
  TITLE     Gene targeting of Gemin2 in mice reveals a correlation between
            defects in the biogenesis of U snRNPs and motoneuron cell death
  JOURNAL   Proc Natl Acad Sci U S A 99 (15), 10126-10131 (2002)
   PUBMED   12091709
  REMARK    GeneRIF: Gene targeting in mice reveals a correlation between
            defects in the biogenesis of U snRNPs and motoneuron cell death
            (gemin2; SMN interacting protein 1)
REFERENCE   9  (bases 1 to 1590)
  AUTHORS   Tucker,K.E., Berciano,M.T., Jacobs,E.Y., LePage,D.F.,
            Shpargel,K.B., Rossire,J.J., Chan,E.K., Lafarga,M., Conlon,R.A. and
            Matera,A.G.
  TITLE     Residual Cajal bodies in coilin knockout mice fail to recruit Sm
            snRNPs and SMN, the spinal muscular atrophy gene product
  JOURNAL   J Cell Biol 154 (2), 293-307 (2001)
   PUBMED   11470819
REFERENCE   10 (bases 1 to 1590)
  AUTHORS   Jablonka,S., Bandilla,M., Wiese,S., Buhler,D., Wirth,B.,
            Sendtner,M. and Fischer,U.
  TITLE     Co-regulation of survival of motor neuron (SMN) protein and its
            interactor SIP1 during development and in spinal muscular atrophy
  JOURNAL   Hum Mol Genet 10 (5), 497-505 (2001)
   PUBMED   11181573
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BB623237.1, AK005928.1 and
            AC153654.4.
            
            On Sep 19, 2015 this sequence version replaced NM_025656.4.
            
            Summary: This gene encodes one of the proteins found in the
            survival of motor neuron (SMN) complex, which consists of the SMN
            protein and several gemin proteins. The SMN complex is localized to
            a subnuclear compartment called gems (gemini of coiled bodies) and
            is required for assembly of spliceosomal small nuclear
            ribonucleoproteins (snRNP) and for pre-mRNA splicing. This protein
            interacts directly with the SMN protein and it is required for
            formation of the SMN complex. Disruption of this gene in mouse
            resulted in impaired snRNP assembly, and motor neuron degeneration.
            [provided by RefSeq, Sep 2015].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK005928.1, AK013414.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-42                BB623237.1         3-44
            43-808              AK005928.1         43-808
            809-809             AC153654.4         55445-55445         c
            810-1588            AK005928.1         810-1588
            1589-1590           AC153654.4         53841-53842         c
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 25.99 cM"
     gene            1..1590
                     /gene="Gemin2"
                     /gene_synonym="gemin-2; Sip1"
                     /note="gem nuclear organelle associated protein 2"
                     /db_xref="GeneID:66603"
                     /db_xref="MGI:MGI:1913853"
     exon            1..223
                     /gene="Gemin2"
                     /gene_synonym="gemin-2; Sip1"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..896
                     /gene="Gemin2"
                     /gene_synonym="gemin-2; Sip1"
                     /note="survival of motor neuron protein-interacting
                     protein 1; component of gems 2; SMN-interacting protein 1;
                     survivor of motor neuron protein interacting protein 1"
                     /codon_start=1
                     /product="gem-associated protein 2"
                     /protein_id="NP_079932.2"
                     /db_xref="CCDS:CCDS36457.1"
                     /db_xref="GeneID:66603"
                     /db_xref="MGI:MGI:1913853"
                     /translation="MAWVPAESAVEELMPRLLPVEPCDLTEGFDPSVPPRTPQEYLRR
                     VQIEAAQCPDVVVAQIDPKKLKRKQSVNISLSGCQPAPEGYSPTLQWQQQQVAHFSTV
                     RQSVHKHRNHWKSQQLDSNVAMPKSEDEEGWKKFCLGERLCAEGATGPSTEESPGIDY
                     VQVGFPPLLSIVSRMNQTTITSVLEYLSNWFGERDFTPELGRWFYALLACLEKPLLPE
                     AHSLIRQLARRCSEVRLLVGSKDDERVPALNLLICLVSRYFDQRDLADEPS"
     exon            224..308
                     /gene="Gemin2"
                     /gene_synonym="gemin-2; Sip1"
                     /inference="alignment:Splign:2.1.0"
     exon            309..398
                     /gene="Gemin2"
                     /gene_synonym="gemin-2; Sip1"
                     /inference="alignment:Splign:2.1.0"
     exon            399..458
                     /gene="Gemin2"
                     /gene_synonym="gemin-2; Sip1"
                     /inference="alignment:Splign:2.1.0"
     exon            459..572
                     /gene="Gemin2"
                     /gene_synonym="gemin-2; Sip1"
                     /inference="alignment:Splign:2.1.0"
     exon            573..617
                     /gene="Gemin2"
                     /gene_synonym="gemin-2; Sip1"
                     /inference="alignment:Splign:2.1.0"
     exon            618..686
                     /gene="Gemin2"
                     /gene_synonym="gemin-2; Sip1"
                     /inference="alignment:Splign:2.1.0"
     exon            687..797
                     /gene="Gemin2"
                     /gene_synonym="gemin-2; Sip1"
                     /inference="alignment:Splign:2.1.0"
     exon            798..856
                     /gene="Gemin2"
                     /gene_synonym="gemin-2; Sip1"
                     /inference="alignment:Splign:2.1.0"
     exon            857..1590
                     /gene="Gemin2"
                     /gene_synonym="gemin-2; Sip1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1044..1049
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gemin2"
                     /gene_synonym="gemin-2; Sip1"
                     /note="hexamer: AATATA"
     polyA_site      1066
                     /gene="Gemin2"
                     /gene_synonym="gemin-2; Sip1"
                     /note="major polyA site"
     regulatory      1488..1493
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gemin2"
                     /gene_synonym="gemin-2; Sip1"
                     /note="hexamer: AATAAA"
     polyA_site      1524
                     /gene="Gemin2"
                     /gene_synonym="gemin-2; Sip1"
     polyA_site      1590
                     /gene="Gemin2"
                     /gene_synonym="gemin-2; Sip1"
ORIGIN      
        1 gtagccggtg gcctcggcgg aggtcgctta ggcgcctgcg ccgtgcctac tctgggcgcc
       61 gattgagact ggcgggagcg agaaccatgg cgtgggttcc agccgagtct gcggtggaag
      121 agttgatgcc acggttgtta cctgtggagc cctgtgactt gacggaaggt ttcgatccct
      181 cggtcccccc caggacgcct caggaatatc tgaggagagt gcagatcgaa gcagcccaat
      241 gtccagatgt tgtggtagcc cagattgacc caaagaagtt gaaaaggaag caaagtgtga
      301 acatttctct ttccggatgc cagcctgcgc ctgaaggtta ctctccaaca cttcagtggc
      361 aacaacaaca agtggcacat ttttcaactg ttcgacagag tgtacacaag catagaaatc
      421 actggaaatc acaacagttg gacagtaatg tggcaatgcc aaaatctgaa gatgaagaag
      481 gctggaaaaa attttgtctg ggtgaaaggt tatgtgctga aggggccact ggaccgtcta
      541 cagaggaaag ccctgggatc gattatgtac aagttggttt tcctcctttg cttagtattg
      601 taagcagaat gaatcagaca accattacta gtgtcttgga atatctaagt aattggtttg
      661 gagaaagaga ctttactcca gaattgggaa gatggtttta tgctttgttg gcttgtcttg
      721 aaaagccttt attacctgag gctcattcac taatccgaca gcttgcaaga agatgttctg
      781 aagtgaggct gctggtgggc agtaaagatg acgaaagagt cccagctttg aatctgctga
      841 tctgcttagt tagcaggtat tttgatcaac gcgatttagc tgatgagcca tcttgatggc
      901 gcttacctcc tagggatgga agatgattct gtggaaggag cctaagtctg agaaacacag
      961 ttccaacctg aggacagatt acatcacttc ttcaagttat gtgaagggct tcatcttaat
     1021 ctgcagctca agctgatatt caaaatatag atttgattgg tttaaagtgt ctgaaatgtc
     1081 tgtgaaaaag cccatttggt atctgatgag caattggaac agttttctct ttttttgggg
     1141 ggtggggggt ggtggatatg gagacagggt ttctctgtgt agccctggct gtcctggaac
     1201 tcactctgta gaccaggctg gcctcaaact cagaaatctg cctgcctctg cctcccgagt
     1261 gctgggatta aaggagtgcg acaccaccgc cgggctggaa gttttcttaa gttgcatgca
     1321 ttgaaaagag gtcaagtgtg acttactact taagcagctt gtataaaaac tgatggcatt
     1381 ttatacaggc ccctggggct gcttcattgg gtttttttta caagtataca tatatattta
     1441 tgttgaaatg tattttgtct atggaaattt ttttgactct tgaacaaaat aaaagacact
     1501 ggatgtcttt agagttgaag ttcatacaga tgaaatggca aatctgttca tagtcgacaa
     1561 gactaatcca gtgttcatgt ttaaactcaa
//
