LOCUS       NM_026572               1247 bp    mRNA    linear   ROD 08-JUN-2024
DEFINITION  Mus musculus glycine cleavage system protein H (aminomethyl
            carrier) (Gcsh), transcript variant 1, mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_026572
VERSION     NM_026572.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Peng,M.Z., Shao,Y.X., Li,X.Z., Zhang,K.D., Cai,Y.N., Lin,Y.T.,
            Jiang,M.Y., Liu,Z.C., Su,X.Y., Zhang,W., Jiang,X.L. and Liu,L.
  TITLE     Mitochondrial FAD shortage in SLC25A32 deficiency affects
            folate-mediated one-carbon metabolism
  JOURNAL   Cell Mol Life Sci 79 (7), 375 (2022)
   PUBMED   35727412
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1247)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1247)
  AUTHORS   Leung,K.Y., De Castro,S.C.P., Galea,G.L., Copp,A.J. and
            Greene,N.D.E.
  TITLE     Glycine Cleavage System H Protein Is Essential for Embryonic
            Viability, Implying Additional Function Beyond the Glycine Cleavage
            System
  JOURNAL   Front Genet 12, 625120 (2021)
   PUBMED   33569080
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC162858.6.
            
            On Jun 14, 2018 this sequence version replaced NM_026572.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK050373.1, AK019983.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-211               AC162858.6         94359-94569
            212-291             AC162858.6         98654-98733
            292-355             AC162858.6         100481-100544
            356-487             AC162858.6         103918-104049
            488-1247            AC162858.6         105131-105890
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 63.43 cM"
     gene            1..1247
                     /gene="Gcsh"
                     /gene_synonym="1100001L02Rik; 5730591C18Rik"
                     /note="glycine cleavage system protein H (aminomethyl
                     carrier)"
                     /db_xref="GeneID:68133"
                     /db_xref="MGI:MGI:1915383"
     exon            1..211
                     /gene="Gcsh"
                     /gene_synonym="1100001L02Rik; 5730591C18Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..585
                     /gene="Gcsh"
                     /gene_synonym="1100001L02Rik; 5730591C18Rik"
                     /note="glycine cleavage system H protein, mitochondrial;
                     lipoic acid-containing protein"
                     /codon_start=1
                     /product="glycine cleavage system H protein, mitochondrial
                     precursor"
                     /protein_id="NP_080848.1"
                     /db_xref="CCDS:CCDS22696.1"
                     /db_xref="GeneID:68133"
                     /db_xref="MGI:MGI:1915383"
                     /translation="MSLQVSRSLRVVAYSLRTALTFCSPRPCVPSAAAVRSLRTGSAL
                     LSVRKFTEKHEWITTEEGIGTVGISNFAQEALGDVVYCSLPEVGTKLKKQEEFGALES
                     VKAASELYSPLSGEVTEVNEALAENPGLVNKSCYEDGWLIKMTLSDPSEMDELMSEEA
                     YEKYVKSIEE"
     exon            212..291
                     /gene="Gcsh"
                     /gene_synonym="1100001L02Rik; 5730591C18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            292..355
                     /gene="Gcsh"
                     /gene_synonym="1100001L02Rik; 5730591C18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            356..487
                     /gene="Gcsh"
                     /gene_synonym="1100001L02Rik; 5730591C18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            488..1247
                     /gene="Gcsh"
                     /gene_synonym="1100001L02Rik; 5730591C18Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1222..1227
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gcsh"
                     /gene_synonym="1100001L02Rik; 5730591C18Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1242
                     /gene="Gcsh"
                     /gene_synonym="1100001L02Rik; 5730591C18Rik"
                     /note="major polyA site"
ORIGIN      
        1 actctggacc caccccgtcc ccaccggccc ggtggcggag gcgaagcgca gttaccctcc
       61 ggacctctga ccatgtcgct gcaagtgtcg aggagcttgc gggttgtggc ctacagcctg
      121 cgcaccgccc tgaccttctg ctcgccgcgg ccctgtgtac cgagcgccgc cgccgtccgc
      181 tcactgcgca ccggatctgc tttgctgtcg gtgcgtaaat tcacagagaa acatgagtgg
      241 ataacgacag aggaaggtat tggaacggtg ggaatcagca attttgcaca ggaagcgttg
      301 ggagatgttg tttactgtag tctgcctgaa gttgggacaa aattgaaaaa acaagaggag
      361 tttggtgctt tggagagtgt gaaggctgcc agtgaactct actctcctct gtcaggagag
      421 gtaaccgaag tcaatgaagc tcttgccgaa aacccagggc ttgtcaacaa atcctgttac
      481 gaagatggtt ggctgatcaa gatgacactg agtgaccctt cagagatgga tgaattgatg
      541 agtgaagaag catatgagaa atatgtgaag tccattgagg agtgaccatg gcacccctct
      601 actcccagat caaactggat gtgacgtgct ccagcatgct tgtcttaaac tagtggagaa
      661 tagaggactt gaaataactt agcaatactg atggaaagaa actgttcttg acagtgctac
      721 ttaagagcca cccccaactt cctaaaggat gcagataaac acagtgtaca ggtttttgat
      781 aattgccata ctaagtattt agactagggg ttaagccctg gtgtttagaa ttctgaggat
      841 cgtctgctgt gaaacgtggt tatgaggctg gttttggtga cggaccacct tctaatccca
      901 gcacgcaaca ggcagatgtt agttagttca aggtcggtct acacaacaag ttctagacca
      961 gccacagcta catggcgaga ccctacctac tgggtataag actttcataa ttcaagataa
     1021 tagtggtgta tcttagataa ttttgttact tgcccatatc caccactgga tttgggtcag
     1081 agattagtgt cttctcattg ggaatgcaag gaagtgttgt acagtgtcag aggcaggaga
     1141 gtactgtctt tgtttcacca tgtgctgtgt tgactgttgt tttcccaccg tgaagcaccg
     1201 gctttgcaac actgcgaaac aaataaaaca ttcatcctct ttgttga
//
