LOCUS       NM_001166065            5071 bp    mRNA    linear   ROD 04-AUG-2024
DEFINITION  Mus musculus glucosaminyl (N-acetyl) transferase 4, core 2
            (beta-1,6-N-acetylglucosaminyltransferase) (Gcnt4), transcript
            variant 2, mRNA.
ACCESSION   NM_001166065
VERSION     NM_001166065.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Lan,Z.Z., Sun,F.H., Chen,C., Niu,L., Shi,J.D. and Zhang,W.Y.
  TITLE     CircPRDM5 inhibits the proliferation, migration, invasion, and
            glucose metabolism of gastric cancer cells by reducing GCNT4
            expression in a miR-485-3p-dependent manner
  JOURNAL   Kaohsiung J Med Sci 40 (3), 231-243 (2024)
   PUBMED   38180297
  REMARK    GeneRIF: CircPRDM5 inhibits the proliferation, migration, invasion,
            and glucose metabolism of gastric cancer cells by reducing GCNT4
            expression in a miR-485-3p-dependent manner.
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Kidder,D., Richards,H.E., Ziltener,H.J., Garden,O.A. and
            Crocker,P.R.
  TITLE     Sialoadhesin ligand expression identifies a subset of CD4+Foxp3- T
            cells with a distinct activation and glycosylation profile
  JOURNAL   J Immunol 190 (6), 2593-2602 (2013)
   PUBMED   23408841
REFERENCE   3  (bases 1 to 5071)
  AUTHORS   Mullaly,S.C., Oudhoff,M.J., Min,P.H., Burrows,K., Antignano,F.,
            Rattray,D.G., Chenery,A., McNagny,K.M., Ziltener,H.J. and Zaph,C.
  TITLE     Requirement for core 2 O-glycans for optimal resistance to helminth
            infection
  JOURNAL   PLoS One 8 (3), e60124 (2013)
   PUBMED   23555902
REFERENCE   4  (bases 1 to 5071)
  AUTHORS   Fan,J., Wang,S., Yu,S., He,J., Zheng,W. and Zhang,J.
  TITLE     N-acetylglucosaminyltransferase IVa regulates metastatic potential
            of mouse hepatocarcinoma cells through glycosylation of CD147
  JOURNAL   Glycoconj J 29 (5-6), 323-334 (2012)
   PUBMED   22736280
  REMARK    GeneRIF: N-acetylglucosaminyltransferase IVa regulates metastatic
            potential of mouse hepatocarcinoma cells through glycosylation of
            CD147.
REFERENCE   5  (bases 1 to 5071)
  AUTHORS   Ismail,M.N., Stone,E.L., Panico,M., Lee,S.H., Luu,Y., Ramirez,K.,
            Ho,S.B., Fukuda,M., Marth,J.D., Haslam,S.M. and Dell,A.
  TITLE     High-sensitivity O-glycomic analysis of mice deficient in core 2
            {beta}1,6-N-acetylglucosaminyltransferases
  JOURNAL   Glycobiology 21 (1), 82-98 (2011)
   PUBMED   20855471
REFERENCE   6  (bases 1 to 5071)
  AUTHORS   Stone,E.L., Ismail,M.N., Lee,S.H., Luu,Y., Ramirez,K., Haslam,S.M.,
            Ho,S.B., Dell,A., Fukuda,M. and Marth,J.D.
  TITLE     Glycosyltransferase function in core 2-type protein O glycosylation
  JOURNAL   Mol Cell Biol 29 (13), 3770-3782 (2009)
   PUBMED   19349303
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC174082.3.
            
            On Dec 6, 2019 this sequence version replaced NM_001166065.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK157788.1, SRR9219380.81454.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-236               AC174082.3         164648-164883
            237-353             AC174082.3         175815-175931
            354-5071            AC174082.3         186132-190849
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 50.66 cM"
     gene            1..5071
                     /gene="Gcnt4"
                     /gene_synonym="C2gnt3; Gm279; Gm73"
                     /note="glucosaminyl (N-acetyl) transferase 4, core 2
                     (beta-1,6-N-acetylglucosaminyltransferase)"
                     /db_xref="GeneID:218476"
                     /db_xref="MGI:MGI:2684919"
     exon            1..236
                     /gene="Gcnt4"
                     /gene_synonym="C2gnt3; Gm279; Gm73"
                     /inference="alignment:Splign:2.1.0"
     exon            237..353
                     /gene="Gcnt4"
                     /gene_synonym="C2gnt3; Gm279; Gm73"
                     /inference="alignment:Splign:2.1.0"
     exon            354..5071
                     /gene="Gcnt4"
                     /gene_synonym="C2gnt3; Gm279; Gm73"
                     /inference="alignment:Splign:2.1.0"
     CDS             355..1722
                     /gene="Gcnt4"
                     /gene_synonym="C2gnt3; Gm279; Gm73"
                     /EC_number="2.4.1.102"
                     /note="core 2 beta-1,6-N-acetylglucosaminyltransferase 3;
                     glucosaminyl (N-acetyl) transferase 3, mucin type
                     pseudogene; core 2-branching enzyme 3;
                     core2-GlcNAc-transferase 3"
                     /codon_start=1
                     /product="beta-1,3-galactosyl-O-glycosyl-glycoprotein
                     beta-1,6-N-acetylglucosaminyltransferase 4"
                     /protein_id="NP_001159537.1"
                     /db_xref="CCDS:CCDS49336.1"
                     /db_xref="GeneID:218476"
                     /db_xref="MGI:MGI:2684919"
                     /translation="MKIFRCCFKYTLQQKLFILLLTLWLFSLLKLLNVGRLLFPQRDI
                     YLVEYSLSTSPFVRNRFPESGDAARDNVNCSGVYEHEPLEIGKSLEIRRRSIIDLEDG
                     DVVAMTSDCDVYQTLRQYHEKLVSREEEDFPIAYSLVVHKDAIMVERLIRAIYNQHNL
                     YCIHYDLKSPDTFKAAMNNLAKCFPNIFIASKLETVEYAHISRLQADWNCLSDLLKSS
                     VQWKYVINLCGQDFPLKSNFELVTELKSLQGRNMLETVRPPSAKTERFTYHHELRQVP
                     YDYMKLPVKTNVSKGAPPHNIQVFVGSAYFVLSRAFVKYIFNSSLVEDFFAWSKDTYS
                     PDEHFWATLIRIPGIPGGISSSSQDVSDLQSKTRLVKWFYYEGFLYPNCTGSHLRSVC
                     IYGAAELRWLLNEGHWFANKFDSKVDPILMKCLAEKLEEQQRKLIALSSEKFMTEGTR
                     QSHTL"
     regulatory      5047..5052
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gcnt4"
                     /gene_synonym="C2gnt3; Gm279; Gm73"
                     /note="hexamer: AATAAA"
     polyA_site      5071
                     /gene="Gcnt4"
                     /gene_synonym="C2gnt3; Gm279; Gm73"
                     /note="major polyA site"
ORIGIN      
        1 agagaagcgg gtagcccggc cggagagccg cgccaggaaa cttcggtccc cagtgcggtg
       61 cggggacccc ctgcgggagc acggtggcga gcccctctct tctcccgcgt cacctggggc
      121 ctcggcgtta ccccactccc tgccgaggga gagcgacttc ggggcagtgc gtgtggcccg
      181 gagcaccggg gtggcgcagc agcggggacc ttgcgcccgg gggtctcagc tccccgaccc
      241 gaacgactct ggaacaggca ctgcaggcgg aatagagatc taaggaagca gtatggagaa
      301 tgactttcat gtcctgcagt tccattgagg ccgctgttct tgctgttttg cagaatgaag
      361 atattcagat gttgctttaa atacactctc cagcagaaac tcttcatcct cctcttaacc
      421 ctgtggctgt tctccttgtt gaagctccta aatgtgggca ggctcctctt ccctcaaaga
      481 gacatttact tagttgaata ctccctaagt acatcaccat ttgtgaggaa caggttcccc
      541 gagtctgggg atgcagccag ggacaacgtt aactgctcgg gggtctacga gcacgagcct
      601 ttggaaatcg gcaagagtct agaaatcaga agacggagca tcatcgactt ggaggacggt
      661 gatgtcgtgg cgatgacaag tgactgtgac gtttatcaga ccctaaggca gtaccatgaa
      721 aagctggttt caagggagga agaggacttc cccatagcct attcgctggt cgtccacaaa
      781 gatgccatta tggttgagcg gttgatccga gctatttaca accagcacaa cctttactgc
      841 atccattatg acctgaagtc accggacacg ttcaaagctg ccatgaacaa cctagctaag
      901 tgcttcccca atatcttcat cgcttccaaa ttagagactg tggagtatgc tcacatatcc
      961 aggctccagg ccgattggaa ctgcttatca gacctcctca agtcttccgt tcagtggaag
     1021 tacgtcatca acctctgtgg gcaagacttt cccctaaagt caaattttga attagtgaca
     1081 gagctgaaaa gtctccaagg aaggaatatg ttagagacgg tgagaccccc cagtgctaag
     1141 acggagaggt tcacctacca tcatgagctc agacaggtgc cttatgatta tatgaagcta
     1201 ccagtaaaga cgaacgtctc caagggggca ccccctcata acattcaggt atttgtgggc
     1261 agtgcctatt ttgttttaag tcgagcattt gttaaatata ttttcaacag ctccctcgtt
     1321 gaagactttt ttgcctggtc taaagataca tattctcctg acgagcactt ttgggccacc
     1381 ttaatccgga taccaggaat acccggggga atttccagtt catctcagga cgtgtctgac
     1441 ctgcagagta agacccgcct ggtcaaatgg ttttactacg aaggctttct ctaccccaat
     1501 tgcactggct ctcaccttcg aagtgtgtgt atttacggag ctgcagaact acggtggctc
     1561 ttaaacgaag ggcattggtt tgctaataag tttgattcta aagttgaccc catcttgatg
     1621 aaatgtctgg cagaaaaact tgaagagcaa cagagaaagt tgattgcttt gtcttcagag
     1681 aagttcatga cagagggaac ccgccaaagc cacacattat aagctgacta agggcctgag
     1741 ggtgtctgtt acaaacagtt aagtgagatg gaattacaca cccatgtcct gcccaacagc
     1801 atctgaactt aatagttttt ccaaggatga aacttctacg gatttccatg cctggacttc
     1861 gagcccacag ttggctggtg tttaagtgct gaaattgctt gtcgtcttgc taagtcagat
     1921 atgttaaatg ttcagtgata tcacatttat tgaacaccta acagcgcacc aggcacttta
     1981 tagaaagaca cccaggagtt aagtctaagt tgcaatgact tgtgccctca ggaagcagcc
     2041 tttaccctcc tagtgtggca actgttgtgt ttcttatttc tgtgtaacga gggtgctact
     2101 gaagccaact ttggggtgtg tgctaatcgt acaagccatt ccctctgaaa agtgtgggag
     2161 tgtgaagcca gccctcccct tctctcactt tagcaactga gaccttttta aatcgttaga
     2221 atattaggag gagggattcc actacctcag agaagctcaa agaatggtcc gatcgcctgc
     2281 ttgccaaagt ataatttacc ttttggtgcc tcacttttct gattcagggt agagggagtg
     2341 ctgtggtgtg atgcttgtag ggtatggttc tctgagaagg cagaggggcg ggcgtgggtg
     2401 gatggggaca gccagtgctg gagaggaaca caggctccat tcagctttgc atttgttata
     2461 caatggtgtc acaattggct ttctttttaa ttcacttgtt tctttaaaac acaggtactg
     2521 ataaagataa aactgagaac tagttcaaaa tgtcacaggc cagaactcca ggcctacacc
     2581 tgtcatttca tcaggtaaca ttagagctac aattgtatcc ttgccgcagt ccaacacttc
     2641 tgacagggtt catagttcta tgtaaccgcc aagtcagtaa gctttcttag aaggactttg
     2701 tgaaatatga cagctttctc ggttggttat tgtggtcact tactcatttt ccttttttaa
     2761 acgaaaatgg agtcaaaatt caaaattatt aacacttcct gttagtgcag catttttctc
     2821 aactgagcac caaagagaat attattaccc ttattttaca aacacccaca cttagaatca
     2881 ttttacatgc acacacatac acatgcacac acatacacat gcacacatgc acacatacac
     2941 acacatgcac acggatgggg gaggggcaga ggcaccttga gactgaacgt attgtgtatt
     3001 gtgctgctga gccacaaccc tgagaacagg ctgcaacatc acaccagtct ctgtggaggg
     3061 taggacacat gtaaaaattc cttgtgtttt gatatcagcc ccaataacta attatgtggt
     3121 gatcaatgcc aaaatcagat tatttgaaag actggagtac gtaaatatca ttttacactt
     3181 ctgtgctctg cacgtgccat gctcttggtg tatataatgg ttcgtagcac tagctgccct
     3241 gtggccccat tttgctccat ttctttatct ttaagtcacc ttgcacctct ttcaataccc
     3301 tttggatgct tttgttccca gggaaacgtc tgtcaccaac tttctatcct tagatgatga
     3361 agtatagagg gtggctcggc agttaagaaa atgtgctgct cctgcagaag acccaagttc
     3421 tggtcccagc actcacacca ggtggctccc agctgcctgt aactccagtt gtagggaaac
     3481 cagtgccacc ttctggcctc cagtggcacc tacacccatc agtgtgtaca cacacacaca
     3541 cacacaaatg tactcacaca tacaggcttg cacacgcaca cctgcacaca gatgtattac
     3601 aggtttttag gagcctcaca gagaatctgc ccaggtctgt gggtgggtga gacctaagaa
     3661 aaccatccta tcggcctcca tgggctctgc tctctgtgca ctgagcacag gcactgaaca
     3721 tgctgtcgtt tcctcggagg ctctcatgag cttcaaggga aatgtcacat gaaggggaga
     3781 caaaatttga actatcagtt tggtgacagt gtcaagataa ccaggtccat tgtgagaatt
     3841 gaaaatgagc ctttaaacca caagaggcca gagttgcagg ggaagctctg ccaccagatt
     3901 cagggctgag aaaaaaaaaa ccctgtcatg gcatttggag tttcttgtac agccaacagc
     3961 atagtagcat gcagattaga gaaacgttga taaccatgca tgatcaaact caaggacttc
     4021 ggtcatcaag gagaatgtaa gggcaacatg cagcaagcat ctaccttgtc ccctgtcttt
     4081 ctgcagagaa aggcccctgt tctctggtat taggaattgt ctcaaattgc tgcatgctgc
     4141 cctgctattt tcaagtggat gatgggcaga ccaagtggca tgccttcctt cccccatgga
     4201 cacatttctc tggactcaga aacgtcaacc gcttgtattt cagagagctc gccaaagcac
     4261 tggctcttgc cttgggaatc ctgcagggtc ttcagaattg attctggcaa aaaccgtttg
     4321 ctatgagccc tccccaaatc tcagaatgta caacactgtt tgataattgt tctctgtata
     4381 gtatttattg aattagactc taatttactt aaaggtgaga gtttacgaat tgtatgtgaa
     4441 tactagaaga aaaatgaagg ctgtcaaaat atgtgagcaa tcgtatactg taccatagac
     4501 actgtatatc gaaaagccag tactgctatt tttttctttt gcctacttta aataataaaa
     4561 cacattgaaa gattaaagac tttagggcta gaaagatgcc ttagcagtta agagtactgg
     4621 tcacttgtcc agaggatcca aattcaattc ccagaatcct catggcagct tacaaccatc
     4681 tgtaactcca gtttgaggag acctggtgtc cctcctcttc tggcctcaga aagcacaaac
     4741 acacacatgg tagactgaca tacttgcagg caaaacatgc acataaaata agttgaaaat
     4801 taaagctgtc tcttggcttc cagaatgtga atatatacag gcttaagtat ttcatgtgaa
     4861 acgaatgtat taagtatatt caaattgttt ctgtgaatgg tacaatgttt tccttgtcac
     4921 tctctatggc tcctttaccg tgttgtcctt gtcccttacc attttagtaa caaatctccc
     4981 atgtatgggt tttgaatagt gatttttttt gtttatttaa attatgttag ttttctggca
     5041 ttgcaaaata aaatgtgctc ctcaaatcca a
//
