LOCUS       NM_028087               4301 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus glucosaminyl (N-acetyl) transferase 3, mucin type
            (Gcnt3), mRNA.
ACCESSION   NM_028087
VERSION     NM_028087.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Soulez,M., Saba-El-Leil,M.K., Turgeon,B., Mathien,S., Coulombe,P.,
            Klinger,S., Rousseau,J., Levesque,K. and Meloche,S.
  TITLE     Reevaluation of the Role of Extracellular Signal-Regulated Kinase 3
            in Perinatal Survival and Postnatal Growth Using New Genetically
            Engineered Mouse Models
  JOURNAL   Mol Cell Biol 39 (6), e00527-18 (2019)
   PUBMED   30642949
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Rao,C.V., Janakiram,N.B., Madka,V., Kumar,G., Scott,E.J.,
            Pathuri,G., Bryant,T., Kutche,H., Zhang,Y., Biddick,L., Gali,H.,
            Zhao,Y.D., Lightfoot,S. and Mohammed,A.
  TITLE     Small-Molecule Inhibition of GCNT3 Disrupts Mucin Biosynthesis and
            Malignant Cellular Behaviors in Pancreatic Cancer
  JOURNAL   Cancer Res 76 (7), 1965-1974 (2016)
   PUBMED   26880801
REFERENCE   3  (bases 1 to 4301)
  AUTHORS   Kidder,D., Richards,H.E., Ziltener,H.J., Garden,O.A. and
            Crocker,P.R.
  TITLE     Sialoadhesin ligand expression identifies a subset of CD4+Foxp3- T
            cells with a distinct activation and glycosylation profile
  JOURNAL   J Immunol 190 (6), 2593-2602 (2013)
   PUBMED   23408841
REFERENCE   4  (bases 1 to 4301)
  AUTHORS   Mullaly,S.C., Oudhoff,M.J., Min,P.H., Burrows,K., Antignano,F.,
            Rattray,D.G., Chenery,A., McNagny,K.M., Ziltener,H.J. and Zaph,C.
  TITLE     Requirement for core 2 O-glycans for optimal resistance to helminth
            infection
  JOURNAL   PLoS One 8 (3), e60124 (2013)
   PUBMED   23555902
REFERENCE   5  (bases 1 to 4301)
  AUTHORS   Ismail,M.N., Stone,E.L., Panico,M., Lee,S.H., Luu,Y., Ramirez,K.,
            Ho,S.B., Fukuda,M., Marth,J.D., Haslam,S.M. and Dell,A.
  TITLE     High-sensitivity O-glycomic analysis of mice deficient in core 2
            {beta}1,6-N-acetylglucosaminyltransferases
  JOURNAL   Glycobiology 21 (1), 82-98 (2011)
   PUBMED   20855471
REFERENCE   6  (bases 1 to 4301)
  AUTHORS   Stone,E.L., Ismail,M.N., Lee,S.H., Luu,Y., Ramirez,K., Haslam,S.M.,
            Ho,S.B., Dell,A., Fukuda,M. and Marth,J.D.
  TITLE     Glycosyltransferase function in core 2-type protein O glycosylation
  JOURNAL   Mol Cell Biol 29 (13), 3770-3782 (2009)
   PUBMED   19349303
REFERENCE   7  (bases 1 to 4301)
  AUTHORS   Hashimoto,M., Tan,S., Mori,N., Cheng,H. and Cheng,P.W.
  TITLE     Mucin biosynthesis: molecular cloning and expression of mouse
            mucus-type core 2 beta1,6 N-acetylglucosaminyltransferase
  JOURNAL   Glycobiology 17 (9), 994-1006 (2007)
   PUBMED   17591617
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from EF202835.1.
            
            [WARNING] On Aug 6, 2024 this sequence was replaced by NM_028087.3.
            
            On Feb 4, 2007 this sequence version replaced NM_028087.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EF202835.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 39.2 cM"
     gene            1..4301
                     /gene="Gcnt3"
                     /gene_synonym="2010013H22Rik; 2210021I22Rik;
                     2210401J11Rik"
                     /note="glucosaminyl (N-acetyl) transferase 3, mucin type"
                     /db_xref="GeneID:72077"
                     /db_xref="MGI:MGI:1919327"
     exon            1..116
                     /gene="Gcnt3"
                     /gene_synonym="2010013H22Rik; 2210021I22Rik;
                     2210401J11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            117..458
                     /gene="Gcnt3"
                     /gene_synonym="2010013H22Rik; 2210021I22Rik;
                     2210401J11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            459..4301
                     /gene="Gcnt3"
                     /gene_synonym="2010013H22Rik; 2210021I22Rik;
                     2210401J11Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             513..1826
                     /gene="Gcnt3"
                     /gene_synonym="2010013H22Rik; 2210021I22Rik;
                     2210401J11Rik"
                     /EC_number="2.4.1.102"
                     /EC_number="2.4.1.150"
                     /EC_number="2.4.1.148"
                     /note="core 2 beta-1,6-N-acetylglucosaminyltransferase II;
                     C2GnT-M; C2GnT-mucin type; mucus-type core 2
                     beta-1,6-N-acetylglucosaminyltransferase"
                     /codon_start=1
                     /product="beta-1,3-galactosyl-O-glycosyl-glycoprotein
                     beta-1,6-N-acetylglucosaminyltransferase 3"
                     /protein_id="NP_082363.2"
                     /db_xref="CCDS:CCDS40678.1"
                     /db_xref="GeneID:72077"
                     /db_xref="MGI:MGI:1919327"
                     /translation="MTSWQRLCWHYRLWTLGCYMLLAILALKLSLRLKCDFDAMDLDS
                     EEFQSQYCRDLLYKTLKLPAKSSINCSGVIRGEQKAVTQALLNNLEIKKKQQLFTEAD
                     YLRMTADCEHFKTKRKFIQVPLSKEEASFPIAYSMVVHEKIENFERLLRAVYTPQNVY
                     CVHMDQKSSEPFKQAVRAIVSCFPNVFIASKLVSVVYASWSRVQADLNCMEDLLQSPV
                     PWKYLLNTCGTDFPIKTNAEMVKALKLLKGQNSMESEVPPPHKKSRWKYHYEVTDTLH
                     MTSKRKTPPPNNLTMFTGNAYMVASRDFIEHVFSNSKARQLIEWVKDTYSPDEHLWAT
                     LQRASWMPGSDPLHRKFDLSDMRAIARLTKWYDHEGDIENGAPYTSCSGIHQRAVCVY
                     GSGDLHWILQNHHLLANKFDPKVDDNVLQCLEEYLRHKAIYGTEL"
     regulatory      2234..2239
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gcnt3"
                     /gene_synonym="2010013H22Rik; 2210021I22Rik;
                     2210401J11Rik"
                     /note="hexamer: CATAAA"
     polyA_site      2255
                     /gene="Gcnt3"
                     /gene_synonym="2010013H22Rik; 2210021I22Rik;
                     2210401J11Rik"
                     /note="major polyA site"
ORIGIN      
        1 agacatttca gactagtacc ctagctcttc tcactttccc aggtgctcat cctctttata
       61 agggacaaag tctttccctt ggctcctcca ggcaatgcaa cataacagcc ctggcaatgt
      121 ggccagcatt gttcctgtct ccttcgcctc ccagaatgac tagtgcttga gactacaatc
      181 ctgaatggtt tgcttaagtt cactccctcc tctgagaaca ggaagtcaaa aggtgagtga
      241 cagagccacc tgctgctctc tcctttgcat aggtctaaca ggagcctggg tgagctcctt
      301 ggatggctgg tgaacaggag caccttatct atctgggagg tacgaaggca aaggttgtac
      361 tgttaatttg cacctcaaca caaccaacct taaccttcac tccacatcac tcacggcgtt
      421 ggagaaagct ctcctaagca gccaagaagg taccaaagag aacccactgc cctcctctgc
      481 tgggcaaggc ttgatggtcc tcgcctgtga agatgacttc ctggcagagg ctctgctggc
      541 actatcgcct gtggaccctg ggttgctaca tgctactggc catccttgcc ctgaaactgt
      601 ccctcagact gaagtgtgac ttcgatgcca tggatctgga ctctgaggaa tttcaaagcc
      661 agtactgcag ggatctcctg tacaagaccc tgaagctgcc agccaagagt tccatcaact
      721 gctcaggggt cattcgaggg gagcagaaag cggtgaccca ggctctgctg aataacctgg
      781 aaattaagaa gaagcagcag ctcttcacag aggccgacta ccttaggatg acagcagact
      841 gtgagcactt caagaccaag aggaagttta tacaggtccc actgagcaag gaagaggcca
      901 gcttccccat tgcgtactcc atggtggtgc atgagaagat tgagaacttc gaaaggttgc
      961 tgcgagctgt gtacacccct cagaatgtat actgtgtcca catggatcag aagtcttcag
     1021 aaccctttaa gcaggcagtc agggccatcg tgtcatgctt ccccaatgtc ttcatagcta
     1081 gtaagttggt gtcagtggtc tatgcttcct ggtccagggt gcaggctgac ctaaactgca
     1141 tggaagactt gcttcagagc cccgtgccat ggaaatacct cctgaacacc tgtgggacag
     1201 actttcccat caaaaccaat gctgagatgg tcaaggccct caagctattg aaagggcaga
     1261 acagtatgga gtcagaggta ccacctccac ataaaaaatc ccgctggaaa tatcactatg
     1321 aggtgacaga cacattgcac atgaccagca agaggaagac gccgccacct aataacctaa
     1381 ccatgttcac tgggaatgcc tacatggtgg cttctcgaga cttcattgaa cacgtgttca
     1441 gtaactcaaa agcccggcaa ctgatcgagt gggtaaaaga cacctatagt cccgatgagc
     1501 acctttgggc caccctccag cgtgcctcgt ggatgcctgg atcagatccc ttgcatcgaa
     1561 aatttgacct gtcagacatg agagccattg cgagactaac caagtggtac gaccatgagg
     1621 gagacattga gaacggggca ccttacacgt cttgctcagg aatccaccag cgggctgtct
     1681 gtgtttatgg gtcaggggac ctgcactgga tacttcagaa ccatcacctc ttggccaaca
     1741 agtttgaccc aaaggtggat gataatgttc ttcagtgttt agaagaatat ttacgtcaca
     1801 aagccatcta tgggactgaa ctatgagaca ctgtaagact attgttacct gtgtggcaag
     1861 aaaatgccag gacctactgg ggcagtgtgg ggtagatagc tggggctcta gactccatgt
     1921 gtcccttagg atgagggggc tgctattgga cagcgggtaa gtagacctct gctccttgtg
     1981 agctgtcgct ggtgaaggtg gctattctcc agctccctcc ccaacttctt tggcttcctc
     2041 gcagagctag aacatgaaat actgttactt gggataataa gggaggggac atgtgaccag
     2101 ggaccctgga ttttggctga agacttcctg tcaggttctc tacagagact ttgtttcaaa
     2161 tacctctggg gggttggtct tgggtgggga actttagtgc aaagagcccc cctcttttat
     2221 attgttaatt taacataaag agcttctaaa ccaaagccct gcccctccag tgtcatctgt
     2281 gaatccagaa ataatgtaag caggaaaaat atgtgaggca ttactcgtgt gctgaggaca
     2341 gttagtgtct ggtggctggg aagaagtctc cgtagcaagc cgagtagaag gcaaaccgaa
     2401 tcacagagct aattctctgt tactgaaagc cttcctagtc ttgctttcct tggtagattc
     2461 tgaattttcc atgaggggaa gaacagagtg attggactgg tttgctgagt atgtgcttag
     2521 cttcacggcc aaagctcagc atcccgtgcc cagcagccaa tcttactgga gcttagtttg
     2581 gttgggggta gaggtggcgc tgttaatcat taggtccggg agtgaaagga aacggttttg
     2641 ctaacctaag atgcctgaga attctgttta aacctcttga atgaaagctt ctttatttca
     2701 ttgtgtgaat atcagtctcg ctgtttgcca ctttgtagat tgtgccattt ccctgtgggg
     2761 acacagtaag gtgtgtaaat acatatgcac aaatgctaaa ctatgtacat ttacaggcac
     2821 acactcccca gtgaaatcac ctcttctctg aaaacccaga gctaattttt ggcctgtatg
     2881 cattccagtc tgtaaggagc aaggggcgct aaaagcagaa cctttgacta tggtttagct
     2941 ggcttcctgt gtctatgtcc ccgaagaggg ggttatgtac gaattaactt gtgtaatttg
     3001 aatgaggctc ccatcaatgt gttagaatgc taacaggttt ctccgtttca attcttgatc
     3061 cagttaagat tttctctcta cagtcaactt ttattgtagc cttgtccctt taacagttac
     3121 tgtgtgatgg gaatgtcttt tttttttttt ttttcttttt gatttccggg cagggtttca
     3181 cccatatagc tctggctgtc ctggaactct gtagacaggc ctggcttcca actcagagat
     3241 ccacctgcct ctgcctcctc agtgctggga ttaaagagat gcaccaccat gcctagtgga
     3301 atttctattt caaagtaagc aacttccttt tttttttttt ttttttttta agattggctt
     3361 ctgtgggtaa tgggcattgt caccacaaac ttacccaaca cagcaatatt gagtccttgc
     3421 ccgcaacctt acctcagagg tgacagtgcc atgaaatcag aagtcttcag aacccttcag
     3481 ccagcagctg tgactattcc tggcccttcc ctcaggagga aagaagaggg gctgctggcc
     3541 tgtgggatgt gtgtagaaac acctccttaa agacatatat cctgagaaaa gaagccagat
     3601 tcttcatggt caagttttcc ccaaagcttt tgtaataaac tgagcagtag ctttccatag
     3661 aaaagctatc gggttgtggc tctgttaggt tttgggaatt ttcccaacca ttaaaaaaat
     3721 ttttttttct agaaaatgtg ttgggtggat gaaagtagtt tgttcaatag catgaggtca
     3781 tccaatcaga aggatcacgc ttcgcctggt ttgtttagtg tacattcaag gtcacatagg
     3841 caacccagca catctgttca tctttccctt ttgaggcttc tctgtttcat taacccttct
     3901 ctggaaagga aaagtcccgg aaacaaagca agctgttaca cttggcccac cttccaaggc
     3961 ttaagtttcc tccttatttt gatgcatggc catcactgaa ctgtacttta ttttattttt
     4021 ttgctgctca ttctatctgt tcttggttta gtgaacgttt actaagaaaa gaaaaagcag
     4081 ttgttaactg tggggcatat aattcagatg aagaggtcag atacactgtt taaaaatgga
     4141 gagactaata tcttgtctgc cttaaaacgc cttcactgac caaaccaaag gtctatcctt
     4201 atgtatctcg ctcatttgga atttattgca aaggtagaat aagaaatgat tgttcatcag
     4261 tcttgatgtg ctgacaacta ataaaacaat tcattccaca c
//
