LOCUS       NM_023887               4170 bp    mRNA    linear   ROD 26-NOV-2024
DEFINITION  Mus musculus glucosaminyl (N-acetyl) transferase 2 (I blood group)
            (Gcnt2), transcript variant 2, mRNA.
ACCESSION   NM_023887
VERSION     NM_023887.5
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Henion,T.R. and Schwarting,G.A.
  TITLE     N-linked polylactosamine glycan synthesis is regulated by
            co-expression of beta3GnT2 and GCNT2
  JOURNAL   J Cell Physiol 229 (4), 471-478 (2014)
   PUBMED   24105809
  REMARK    GeneRIF: These data clearly suggest that GCNT2 functions in vivo
            together with beta3GnT2 to determine poly-N-acetyllactosamine (PLN)
            levels in olfactory neurons by regulating beta1,6-branches that
            promote PLN extension.
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Zhang,H., Meng,F., Wu,S., Kreike,B., Sethi,S., Chen,W., Miller,F.R.
            and Wu,G.
  TITLE     Engagement of I-branching {beta}-1,
            6-N-acetylglucosaminyltransferase 2 in breast cancer metastasis and
            TGF-{beta} signaling
  JOURNAL   Cancer Res 71 (14), 4846-4856 (2011)
   PUBMED   21750175
REFERENCE   3  (bases 1 to 4170)
  AUTHORS   Revil,T., Gaffney,D., Dias,C., Majewski,J. and Jerome-Majewska,L.A.
  TITLE     Alternative splicing is frequent during early embryonic development
            in mouse
  JOURNAL   BMC Genomics 11, 399 (2010)
   PUBMED   20573213
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4170)
  AUTHORS   Hashimoto,M., Tan,S., Mori,N., Cheng,H. and Cheng,P.W.
  TITLE     Mucin biosynthesis: molecular cloning and expression of mouse
            mucus-type core 2 beta1,6 N-acetylglucosaminyltransferase
  JOURNAL   Glycobiology 17 (9), 994-1006 (2007)
   PUBMED   17591617
  REMARK    GeneRIF: molecular cloning and analysis of expression of mouse
            mucus-type core 2 beta1,6 N-acetylglucosaminyltransferase
REFERENCE   5  (bases 1 to 4170)
  AUTHORS   Bailey,P.J., Klos,J.M., Andersson,E., Karlen,M., Kallstrom,M.,
            Ponjavic,J., Muhr,J., Lenhard,B., Sandelin,A. and Ericson,J.
  TITLE     A global genomic transcriptional code associated with CNS-expressed
            genes
  JOURNAL   Exp Cell Res 312 (16), 3108-3119 (2006)
   PUBMED   16919269
REFERENCE   6  (bases 1 to 4170)
  AUTHORS   Chen,G.Y., Kurosawa,N. and Muramatsu,T.
  TITLE     A novel variant form of murine beta-1,
            6-N-acetylglucosaminyltransferase forming branches in
            poly-N-acetyllactosamines
  JOURNAL   Glycobiology 10 (10), 1001-1011 (2000)
   PUBMED   11030746
REFERENCE   7  (bases 1 to 4170)
  AUTHORS   Magnet,A.D. and Fukuda,M.
  TITLE     Expression of the large I antigen forming
            beta-1,6-N-acetylglucosaminyltransferase in various tissues of
            adult mice
  JOURNAL   Glycobiology 7 (2), 285-295 (1997)
   PUBMED   9134435
REFERENCE   8  (bases 1 to 4170)
  AUTHORS   Granovsky,M., Fode,C., Warren,C.E., Campbell,R.M., Marth,J.D.,
            Pierce,M., Fregien,N. and Dennis,J.W.
  TITLE     GlcNAc-transferase V and core 2 GlcNAc-transferase expression in
            the developing mouse embryo
  JOURNAL   Glycobiology 5 (8), 797-806 (1995)
   PUBMED   8720078
REFERENCE   9  (bases 1 to 4170)
  AUTHORS   Pilz,A., Woodward,K., Povey,S. and Abbott,C.
  TITLE     Comparative mapping of 50 human chromosome 9 loci in the laboratory
            mouse
  JOURNAL   Genomics 25 (1), 139-149 (1995)
   PUBMED   7774911
REFERENCE   10 (bases 1 to 4170)
  AUTHORS   Lee,J.E., Pintar,J. and Efstratiadis,A.
  TITLE     Pattern of the insulin-like growth factor II gene expression during
            early mouse embryogenesis
  JOURNAL   Development 110 (1), 151-159 (1990)
   PUBMED   1964408
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC159205.2 and AC133496.3.
            
            On Jan 28, 2022 this sequence version replaced NM_023887.4.
            
            Transcript Variant: This variant (2) differs in the 5' exon which
            includes 5' UTR and 5' coding region, compared to variant 1. The
            resulting isoform (B) is of the same size but has a different
            N-terminus when compared to isoform A.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC094572.1, AY435148.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1333              AC159205.2         55468-56800         c
            1334-1429           AC133496.3         136219-136314       c
            1430-4170           AC133496.3         128998-131738       c
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 20.12 cM"
     gene            1..4170
                     /gene="Gcnt2"
                     /gene_synonym="5330430K10Rik; IGnT; IGnTA; IGnTB; IGnTC"
                     /note="glucosaminyl (N-acetyl) transferase 2 (I blood
                     group)"
                     /db_xref="GeneID:14538"
                     /db_xref="MGI:MGI:1100870"
     exon            1..1333
                     /gene="Gcnt2"
                     /gene_synonym="5330430K10Rik; IGnT; IGnTA; IGnTB; IGnTC"
                     /inference="alignment:Splign:2.1.0"
     CDS             415..1620
                     /gene="Gcnt2"
                     /gene_synonym="5330430K10Rik; IGnT; IGnTA; IGnTB; IGnTC"
                     /EC_number="2.4.1.150"
                     /note="isoform B is encoded by transcript variant 2;
                     glucosaminyltransferase, I-branching enzyme;
                     N-acetyllactosaminide
                     beta-1,6-N-acetylglucosaminyl-transferase;
                     beta-1,6-N-acetylglucosaminyltransferase; glucosaminyl
                     (N-acetyl) transferase 2, I-branching enzyme; large I
                     antigen-forming beta-1,6-N-acetylglucosaminyltransferase"
                     /codon_start=1
                     /product="N-acetyllactosaminide
                     beta-1,6-N-acetylglucosaminyl-transferase isoform B"
                     /protein_id="NP_076376.3"
                     /db_xref="CCDS:CCDS36637.1"
                     /db_xref="GeneID:14538"
                     /db_xref="MGI:MGI:1100870"
                     /translation="MGSWKYSLFSLSLIAALMLMFMYDRKLWKNYHFPRAVSNISVLA
                     EVCLQMFSGESFYTADSARKTTLENFTCPEYKIQNHYITETLSEEEARFPLAFTLTIH
                     KDYDTFERLFRAIYMPQNVYCVHVDSKATDTFKEEVRQLLSCFPNAFLASRMEPVVYG
                     GFSRLQADLNCMKDLVASKIPWKYVLNTCGQDFPLKTNKEIVQYLKRFIGKNLTPGVL
                     PPAHAVGRTKYVHQELLDHKNPYVHNTARLKAPPPHNLTIYFGTAYVALTREFANFVL
                     KDQRSVDLISWSKDTYSPDEHFWVTLNRIPGVPGSMPPNASWTGNLRAVKWMDMEAKH
                     GGCHGHYVHGICIYGNGDLQWLINSQSLFANKFELNTYPLTVECLELRLRERTLNQSE
                     IAIQPSWYF"
     exon            1334..1429
                     /gene="Gcnt2"
                     /gene_synonym="5330430K10Rik; IGnT; IGnTA; IGnTB; IGnTC"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..4170
                     /gene="Gcnt2"
                     /gene_synonym="5330430K10Rik; IGnT; IGnTA; IGnTB; IGnTC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4141..4146
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gcnt2"
                     /gene_synonym="5330430K10Rik; IGnT; IGnTA; IGnTB; IGnTC"
                     /note="hexamer: AATAAA"
     polyA_site      4170
                     /gene="Gcnt2"
                     /gene_synonym="5330430K10Rik; IGnT; IGnTA; IGnTB; IGnTC"
                     /note="major polyA site"
ORIGIN      
        1 acagttaaca gccttccact gggagaagac gaggcttggt ttcgagttcc agagcgatgc
       61 ctggccgaaa ggtcactgat agagattttc ccgcgaggac aggtagggtc attggcctct
      121 ggaacctccc tggggtgggg catttttgcg tcgtaattgg ttataagtta cttggaggct
      181 gggcaatgga tctgcttcgg aagtctgtca atgaaaaaaa ggaaacagaa acaagctaag
      241 aaaagggaag ggagagaaga acgagccaag gagctttgct catcagagcc tcatccgtgc
      301 gggatggtac atccgtgcgg gatgatactg ggacctgggg aaaacgtaag ttaaaggaag
      361 ctttgctcca attctgtctc tgaagacact tggctcattt cctgctttgg gaagatgggc
      421 tcttggaagt acagtctttt cagcctatct ctcattgctg ccttgatgct tatgtttatg
      481 tacgatagaa aattatggaa gaactatcat ttcccgaggg cggtttccaa tatttcagtc
      541 ttagcggaag tctgcttgca gatgttcagt ggggagagtt tttatacagc ggacagcgca
      601 cggaaaacca ccctggaaaa cttcacctgc cccgagtaca agattcaaaa ccactacata
      661 acagagactc tctctgaaga agaagcccgc ttccctctgg ctttcacact tactatccac
      721 aaagattatg acactttcga gagactcttc agggccattt acatgcccca gaacgtctac
      781 tgtgtgcacg tggatagtaa ggcgacagac accttcaaag aagaggtgcg gcagttacta
      841 agctgtttcc ccaatgcctt cctggcctct aggatggagc cggtggtcta tggtggcttc
      901 tcccggctcc aagctgatct gaactgcatg aaagatctgg tggcctccaa gatcccctgg
      961 aaatatgtcc tcaacacctg cgggcaggac tttcctctga aaaccaacaa agaaatagtt
     1021 cagtacctga aacggtttat tgggaagaac ctcaccccag gggtgctgcc tcctgcacat
     1081 gcagttggaa gaaccaagta cgtccaccag gagctgttag accataaaaa tccctacgtg
     1141 cacaatacag caagattaaa agctccccca cctcacaacc tgaccattta ctttggcact
     1201 gcttatgtgg ctctcacacg ggaatttgct aactttgtcc tcaaagacca gcgttcagta
     1261 gacttaatct cctggtccaa ggacacgtat agtcctgatg agcacttctg ggtgacactc
     1321 aataggatcc ctggagtccc tggctccatg ccaccaaacg catcctggac gggtaacctc
     1381 agagctgtga agtggatgga catggaagcg aagcatggag gctgccatgg tcactacgtc
     1441 catggcattt gtatctatgg aaacggagac ttgcagtggc tgattaattc gcaaagcctg
     1501 tttgctaaca aatttgaact caacacatac cctcttaccg tggaatgcct ggaactgagg
     1561 cttcgagaaa gaacactcaa ccagagtgag atcgccatac agccgagctg gtatttctga
     1621 cccgcagcag ctcgggccta aatggaaatt gaagacgtaa agaagagctt tcttttccaa
     1681 gagactctgg tcttggctat gctgaagact tttttaaaaa atggttttca gggaaccgtg
     1741 aggatctggc aacatggctc tgcttgcaat atccactgag cactgtaata catttgacag
     1801 gatggctgca tgaaactgtc cagcattttt ctgtcgtttc ccccccaccc caaatccttt
     1861 ttttctcttc ttttttaaaa gataggtagg gtcttgctag atatctctag caagcctgtt
     1921 tcttgtcagg tagcccagga tggccttgaa ctcttaatcc tccgccttgg ccctctaagg
     1981 gctgctagtc cgagtgtgga caaccacacc tggctctggc gtctggcggt cacttgacgc
     2041 tcatctgcgt ctccgtctgc taggtggtcg gtgataaatt caaatgtatc agatctctgt
     2101 gccagatact catattagta taccctgttc aaaggagaat ggtggataat actgtctgag
     2161 gaaatcagaa cttatttgag aaggaagctc tcgttccaca ttcctagcaa ctatattctc
     2221 aggcacacat aggcgcacag agacagcttc ctagtaactc acatcaattt cgtttaatac
     2281 aaactgatcc tgaagagctg aggaactatg taagttcagt gtccaagcat ttgactgaca
     2341 tttgtgcaga gttaggttcc atctccagca tcacgcacgg gtcttgtgaa caccgacatc
     2401 ttcagtttac ttgtgtctgc agttctgtgg acagaacaaa gccattcaca agtgaccgac
     2461 agcacgtgcc gtgagacggg aggtggctgc aggaagagga tgcctcaggg atgccggaca
     2521 tttccgtgtt ctattccaga gtgttctctt ctcccagaga acactggaac gtcctcacag
     2581 ggatgaagga attctttctt tcccacagga aaggtgctac tgtgtgggtc tggaacctgt
     2641 gaatgaatca ctcctggggt ttcgtttcta tgtgagagac ggggacccgg ttctgttgtg
     2701 tgcagcttta gcggcatcag gacagcttcc agcttgcaat gattgctgct gacaggaaca
     2761 cctggatctc aagagctttt tatgacaggt ggacatctgt gtcatttttc cttgttccct
     2821 catccatcca tccatccatc catccatcca tccatccatc catccattcg tcagcctaca
     2881 ttcaacaaac gcctcttgaa tgtctatgat tcccccagac acatgctgac agacacgagg
     2941 gaagcaagac agattctttc tccactgtta aactctgggg agtggtatca gcttttgaaa
     3001 ccctgctcta gactcttaca aaatggctac acttagcaca tactctgccc tggctatata
     3061 ttatattggg ccatagagat cagcaatgta tcaattaata gattaatcca gagacaggaa
     3121 ggaggaaacg aaagctagag cctctttgtg actccaccaa ctccttcttg tctcctttta
     3181 ttgaagagtc cgcacacata ctcacccctc tggtgggcca catgaggacc tgcatcttgc
     3241 agatgatgta gcaagcctta ggatggttca tgatcagtag gaagcaaatt ctaagacctc
     3301 agggtgtctt tctgagtctc tcatttcttt agggatgtgg agagaagatg aagtatagcc
     3361 gtgtgtagtg tcgccagaga aggtggagtt taagaatggt ctcagtgtta ttaatgagaa
     3421 aaagttctgt cattcaggaa aaaaaagcaa aaaacgtgag gttgggactc agtaggtgag
     3481 ggtgcttgca gccaaacctg atgacctgca ttcaattcct gggtcccacg ggacagaggg
     3541 agagagtcca ctactgaaag gaacgctcaa acctcctcac acaggtcctt gtgcctgggc
     3601 actcccccgc cccatatgca tacacacata catgcttttg tgattttttt tattagtgct
     3661 tttacattta gaaatattta tttattctag cagggaaaaa accccacatg ctttaaatga
     3721 tgtcactttc cacaacttca ttcttgtgct gtcattggac tgaatgttat gcatctttaa
     3781 agggaatcac ttcgtgtaaa actctcttgt ctcttctgct gaaaagtaaa atgggttgtg
     3841 tcgggtaata aagggtcgag gggtaggaag cacttggtca aggcgaagtc taaaaccggt
     3901 ctctttttta tttgttactt ggatgagagc acctgaagac taatgtttta cactggattg
     3961 agatctcatg gaaaatgaaa cattcttttt ctggagaagt ttacatgccc attgttggtc
     4021 acctctttac tgtaaatact attgaaaaaa ggagcgtagc atttgcatag cagggattgt
     4081 gcctgtatct actgtgtcca ttttgttttt tcggatgttt tctggttttg tttccttgga
     4141 aataaattaa tatattgttt tccaccttca
//
