LOCUS       NM_173442               4612 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus glucosaminyl (N-acetyl) transferase 1, core 2 (Gcnt1),
            transcript variant 1, mRNA.
ACCESSION   NM_173442
VERSION     NM_173442.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Russo,A.J., Vasudevan,S.O., Mendez-Huergo,S.P., Kumari,P.,
            Menoret,A., Duduskar,S., Wang,C., Perez Saez,J.M., Fettis,M.M.,
            Li,C., Liu,R., Wanchoo,A., Chandiran,K., Ruan,J., Vanaja,S.K.,
            Bauer,M., Sponholz,C., Hudalla,G.A., Vella,A.T., Zhou,B.,
            Deshmukh,S.D., Rabinovich,G.A. and Rathinam,V.A.
  TITLE     Intracellular immune sensing promotes inflammation via gasdermin
            D-driven release of a lectin alarmin
  JOURNAL   Nat Immunol 22 (2), 154-165 (2021)
   PUBMED   33398185
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Fonseca,K.L., Maceiras,A.R., Matos,R., Simoes-Costa,L., Sousa,J.,
            Ca,B., Barros,L., Fernandes,A.I., Mereiter,S., Reis,R., Gomes,J.,
            Tapia,G., Rodriguez-Martinez,P., Martin-Cespedes,M., Vashakidze,S.,
            Gogishvili,S., Nikolaishvili,K., Appelberg,R., Gartner,F.,
            Rodrigues,P.N.S., Vilaplana,C., Reis,C.A., Magalhaes,A. and
            Saraiva,M.
  TITLE     Deficiency in the glycosyltransferase Gcnt1 increases
            susceptibility to tuberculosis through a mechanism involving
            neutrophils
  JOURNAL   Mucosal Immunol 13 (5), 836-848 (2020)
   PUBMED   32203062
  REMARK    GeneRIF: Deficiency in the glycosyltransferase Gcnt1 increases
            susceptibility to tuberculosis through a mechanism involving
            neutrophils.
REFERENCE   3  (bases 1 to 4612)
  AUTHORS   Christians,A., Weiss,A.C., Martens,H., Klopf,M.G., Hennies,I.,
            Haffner,D., Kispert,A. and Weber,R.G.
  TITLE     Inflammation-like changes in the urothelium of Lifr-deficient mice
            and LIFR-haploinsufficient humans with urinary tract anomalies
  JOURNAL   Hum Mol Genet 29 (7), 1192-1204 (2020)
   PUBMED   32179912
REFERENCE   4  (bases 1 to 4612)
  AUTHORS   Perkey,E., Maurice De Sousa,D., Carrington,L., Chung,J., Dils,A.,
            Granadier,D., Koch,U., Radtke,F., Ludewig,B., Blazar,B.R.,
            Siebel,C.W., Brennan,T.V., Nolz,J., Labrecque,N. and Maillard,I.
  TITLE     GCNT1-Mediated O-Glycosylation of the Sialomucin CD43 Is a
            Sensitive Indicator of Notch Signaling in Activated T Cells
  JOURNAL   J Immunol 204 (6), 1674-1688 (2020)
   PUBMED   32060138
REFERENCE   5  (bases 1 to 4612)
  AUTHORS   Carlow,D.A., Tra,M.C. and Ziltener,H.J.
  TITLE     A cell-extrinsic ligand acquired by activated T cells in lymph node
            can bridge L-selectin and P-selectin
  JOURNAL   PLoS One 13 (10), e0205685 (2018)
   PUBMED   30379850
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4612)
  AUTHORS   Chou,D.K. and Jungalwala,F.B.
  TITLE     N-Acetylglucosaminyl transferase regulates the expression of the
            sulfoglucuronyl glycolipids in specific cell types in cerebellum
            during development
  JOURNAL   J Biol Chem 271 (46), 28868-28874 (1996)
   PUBMED   8910533
REFERENCE   7  (bases 1 to 4612)
  AUTHORS   Granovsky,M., Fode,C., Warren,C.E., Campbell,R.M., Marth,J.D.,
            Pierce,M., Fregien,N. and Dennis,J.W.
  TITLE     GlcNAc-transferase V and core 2 GlcNAc-transferase expression in
            the developing mouse embryo
  JOURNAL   Glycobiology 5 (8), 797-806 (1995)
   PUBMED   8720078
REFERENCE   8  (bases 1 to 4612)
  AUTHORS   Miyoshi,E., Nishikawa,A., Ihara,Y., Saito,H., Uozumi,N.,
            Hayashi,N., Fusamoto,H., Kamada,T. and Taniguchi,N.
  TITLE     Transforming growth factor beta up-regulates expression of the
            N-acetylglucosaminyltransferase V gene in mouse melanoma cells
  JOURNAL   J Biol Chem 270 (11), 6216-6220 (1995)
   PUBMED   7890758
REFERENCE   9  (bases 1 to 4612)
  AUTHORS   Sekine,M., Hashimoto,Y., Suzuki,M., Inagaki,F., Takio,K. and
            Suzuki,A.
  TITLE     Purification and characterization of UDP-GlcNAc:IV3 beta Gal-Gb4Cer
            beta-1,6-GlcNAc transferase from mouse kidney
  JOURNAL   J Biol Chem 269 (49), 31143-31148 (1994)
   PUBMED   7983056
REFERENCE   10 (bases 1 to 4612)
  AUTHORS   Spies,T. and DeMars,R.
  TITLE     Restored expression of major histocompatibility class I molecules
            by gene transfer of a putative peptide transporter
  JOURNAL   Nature 351 (6324), 323-324 (1991)
   PUBMED   2034277
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC147369.3, AK045216.1 and BY431975.1.
            
            On Jun 7, 2013 this sequence version replaced NM_173442.4.
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1, 2 and 3 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC039126.1, AK045216.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                AC147369.3         56619-56668         c
            51-3036             AK045216.1         2-2987
            3037-4476           AC147369.3         26272-27711         c
            4477-4612           BY431975.1         300-435
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 12.75 cM"
     gene            1..4612
                     /gene="Gcnt1"
                     /gene_synonym="5630400D21Rik; B130048E03; C2 GlcNAcT;
                     C2GlcNAcT; C2GNT; C2GnT-L; IGnT"
                     /note="glucosaminyl (N-acetyl) transferase 1, core 2"
                     /db_xref="GeneID:14537"
                     /db_xref="MGI:MGI:95676"
     exon            1..111
                     /gene="Gcnt1"
                     /gene_synonym="5630400D21Rik; B130048E03; C2 GlcNAcT;
                     C2GlcNAcT; C2GNT; C2GnT-L; IGnT"
                     /inference="alignment:Splign:2.1.0"
     exon            112..258
                     /gene="Gcnt1"
                     /gene_synonym="5630400D21Rik; B130048E03; C2 GlcNAcT;
                     C2GlcNAcT; C2GNT; C2GnT-L; IGnT"
                     /inference="alignment:Splign:2.1.0"
     exon            259..4606
                     /gene="Gcnt1"
                     /gene_synonym="5630400D21Rik; B130048E03; C2 GlcNAcT;
                     C2GlcNAcT; C2GNT; C2GnT-L; IGnT"
                     /inference="alignment:Splign:2.1.0"
     CDS             388..1674
                     /gene="Gcnt1"
                     /gene_synonym="5630400D21Rik; B130048E03; C2 GlcNAcT;
                     C2GlcNAcT; C2GNT; C2GnT-L; IGnT"
                     /EC_number="2.4.1.102"
                     /note="core 2-branching enzyme; core2-GlcNAc-transferase;
                     beta-1,3-galactosyl-O-glycosyl-glycoprotein
                     beta-1,6-N-acetylglucosaminyltransferase (Core 2 branching
                     enzyme) (Core2-GlcNAc-transferase) (C2GNT); core 2
                     beta-1,6-N-acetylglucosaminyltransferase; leukocyte type
                     core 2 beta-1,6-N-acetylglucosaminyltransferase"
                     /codon_start=1
                     /product="beta-1,3-galactosyl-O-glycosyl-glycoprotein
                     beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="NP_775618.3"
                     /db_xref="CCDS:CCDS29687.1"
                     /db_xref="GeneID:14537"
                     /db_xref="MGI:MGI:95676"
                     /translation="MLRNLFRRRLFSCPTKYYFMLLVLSLITFSVLRIHQKPEFFSVR
                     HLELAGDDPYSNVNCTKILQGDPEEIQKVKLEILTVQFKKRPRRTPHDYINMTRDCAS
                     FIRTRKYIVEPLTKEEVGFPIAYSIVVHHKIEMLDRLLRAIYMPQNFYCIHVDRKAEE
                     SFLAAVQGIASCFDNVFVASQLESVVYASWSRVKADLNCMKDLYRMNANWKYLINLCG
                     MDFPIKTNLEIVRKLKCSTGENNLETEKMPPNKEERWKKRYTVVDGKLTNTGIVKAPP
                     PLKTPLFSGSAYFVVTREYVGYVLENENIQKLMEWAQDTYSPDEFLWATIQRIPEVPG
                     SFPSSNKYDLSDMNAIARFVKWQYFEGHVSNGAPYPPCSGVHVRSVCVFGAGDLSWML
                     RQHHLFANKFDMDVDPFAIQCLDEHLRHKALENLEH"
     regulatory      4581..4586
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gcnt1"
                     /gene_synonym="5630400D21Rik; B130048E03; C2 GlcNAcT;
                     C2GlcNAcT; C2GNT; C2GnT-L; IGnT"
                     /note="hexamer: AATAAA"
     polyA_site      4606
                     /gene="Gcnt1"
                     /gene_synonym="5630400D21Rik; B130048E03; C2 GlcNAcT;
                     C2GlcNAcT; C2GNT; C2GnT-L; IGnT"
                     /note="major polyA site"
ORIGIN      
        1 agggcgtggc gctggctcgg aggtctggag cgcagaaggc tgagcgcgcc agtatccgct
       61 tggcgagccg atccagcact gtttcgtgcg ccctgcaggc acaggctcca ggctgttgga
      121 aagcctgctg ggacatgtca tcgctggatg cctggtgctt gataggaact tggcagcaca
      181 atattccctc tgagcaagta caggatgtca ccaggagtca gagcctcaac agattcaggc
      241 ttcctgtgat tctaaacgtg atatcttgtc ccgctgttaa actggcatct cacctggcca
      301 catcaggaca ctggaggaaa catcattggc attcaaggcc tagaagtttg tcccaccaca
      361 ccaggaagcc tctgaatgtt ctttgaaatg ctgagaaact tgtttcggag gagacttttc
      421 tcttgtccta caaaatacta ctttatgctc cttgtcctct ctttaattac cttctctgtt
      481 ttaagaattc atcagaagcc tgaatttttc agtgtcagac acttggagct ggctggagat
      541 gatccttaca gcaatgttaa ttgcaccaag attttacagg gtgacccaga agaaatccag
      601 aaggtgaagc ttgagatact aacagtgcaa ttcaagaagc gcccgaggcg gacaccccat
      661 gactatataa acatgacccg tgactgtgcc tctttcatca ggacacgcaa atatattgtg
      721 gagcccctta ctaaagaaga ggtaggcttt ccaattgcat attccattgt ggttcatcat
      781 aagattgaaa tgcttgacag gctcctgagg gccatctata tgcctcagaa tttctactgc
      841 attcacgtgg acagaaaagc agaggaatcc tttttagccg cggtgcaggg catcgcatcc
      901 tgctttgata atgtctttgt ggccagccag ttggagagtg ttgtttatgc gtcctggagt
      961 cgggttaagg cagacctcaa ctgcatgaag gacctgtaca gaatgaatgc aaactggaag
     1021 tacttgatca atctctgtgg tatggatttc cctattaaaa ccaacctgga aattgtcagg
     1081 aagctcaagt gctccacagg ggaaaacaac ctggaaactg agaagatgcc tcccaacaag
     1141 gaagagagat ggaaaaaaag atacaccgtt gtcgatggga agctgaccaa cactggaata
     1201 gtcaaagcac cgcccccact gaaaactcct ctcttttcag gcagtgccta cttcgtggtc
     1261 actagggaat atgtaggcta cgtgctggaa aatgaaaata ttcaaaagtt gatggaatgg
     1321 gcacaggaca catacagccc agatgagttc ctctgggcca ccatccaaag gatcccagaa
     1381 gtccctggtt ctttcccctc aagcaacaag tatgacttgt cagacatgaa tgccattgct
     1441 aggtttgtca agtggcagta cttcgaaggc catgtttcca acggtgcccc ttatccaccg
     1501 tgcagtggag tccacgtgcg ctctgtgtgc gtcttcggag ctggtgactt gagctggatg
     1561 ctgcgccaac accacctttt tgccaataag tttgacatgg atgtcgaccc ctttgccatc
     1621 cagtgtttgg atgaacatct gaggcataaa gccctggaga acttagaaca ctaagcgctg
     1681 ctggcagccc tggggaagat aaggttgctc aaactgtact ctcatctact tcccatctct
     1741 cctatggggt cctccttggg gtgatgactt taaagtcttc ctgtcaggga aactgcatga
     1801 atcctacaga atatagttac aggagaggct gataccttaa gtgttctaga ggaaatgtga
     1861 ggactgagtt aggatggctg gaggctggga agacttgatg gacagaggga acccaggtga
     1921 ttgggaaaga ggccagtgaa aaatgtcagc ctcgagaact aatcagagaa tccaggtact
     1981 taatctaaat gagatttgat ttatgccaaa agtctcttga agatttaaaa cacgataatt
     2041 ttcttgacat gaataaattt gtagtagcaa ccaatcacaa agatatgatt caccttgtat
     2101 gttttttgga atagaccttc ggttttactc taagttatcc ttgtaaataa tttccttcct
     2161 gctgtgattt tgggtagtga gtctccacgc gtcatctcag ggagcttaaa atatgtttgg
     2221 cacgaatggc tctgtcttca tttttgctta taagtacaca tacatgtttt taaaagcaaa
     2281 tatcggtttc tagttcaccg atatctacga atattcttca gttatttaaa aggagctagc
     2341 tgggtgtgtg gcccctgcct gtgaagcagg gggattgttt aagctcaagt gatagtggca
     2401 aggctgggca agataacaag aactcttctc tttaaaaagg agaagagaat caactaaatt
     2461 ttacagatac gcatttgttc tttatcttgt cttcccaaac tgttcgtact gtcacgctgc
     2521 acacgtttta ctgcttgagg atcctacatc tgtgttttag gtctttgatg tgacctaggc
     2581 acctgtaact agtgcagaaa actagggagg atgaagtagg catccaaggg aagaagggaa
     2641 tctactaagg ccgttgctaa gcccctggca caagtttaag gctagtaatt ggctcggtcc
     2701 caagaaatga tccaattgtg tctcaggtgg tggcattctg gccttgagta ttcatgttat
     2761 ctgctcactg gattttctta acctgctagc tgctagttcc ttcctctctt gtggccttga
     2821 tgagggggaa agaagtagag aagtacacat gtgtcctcat gaagtgtttg aaaagagctg
     2881 tggcattaca gtgtattttc accaaaaggc aaactgtcta gggtttcttc agttctgtct
     2941 ctcctctcca atgcagggga ttggtttctt tgttttctct tagtattatt tagctaaatc
     3001 taaactgttc atccagaatt ctagtgaatg tgaagacttg ccatttgatt ttaaataaga
     3061 atcgttacca tgtaccttta tttttaaaac atagctaact ttaataatac acacacacac
     3121 aaaatcataa tatcagacag ttttaagaca ttcaaagacg gaagtatgtt ttctgtctca
     3181 aagctataaa atattttttc tagtgacaag tcttattaga aaagctgaga atcagtagac
     3241 gagctggggt ttgttttgga ccaatagaaa tgtgttggcc atgggccacc agcaatgatg
     3301 gtgatgcagc tgataccctc tatactggct tctgatttct ttcttttgtc cagtggattt
     3361 gtccaatggt tcttttagta gaaaggcttt tacttatgag acagggcatc ttttaaatct
     3421 aaaaccagtg gttcctggtg acatagcatt tgtgtaaacc gctgtgacct aagcagtttt
     3481 tctttttctt tttccatctt gcaaaacaca cggctaaggc agtagtctag ggatccagca
     3541 agcaggaacc ccagcctggg gtcaccggtg gtcaacatat ctgtcccagt ttgctttata
     3601 ctggaggaac gcttgaactt tgcaaaactg gaaattaatt ttttctaact acaagtactt
     3661 ttttttttaa tcaactgttt gatgttattc atcttaaaca ctcttgttgt agtggggctc
     3721 catttcagtt aaatggcaag tttgaggatt ttttattttt attttttatt attttttttc
     3781 ttttgcccca ctataggagt aaaactaggc tctaggattc cagagcctcg agtcaatgct
     3841 gtgaagcccc atgctttgtg ttacctcacc ttcgcaccat tgcacttgtt gaaccatgtc
     3901 tgatgctgta gtggcccagt gcctgacaca gggcttgtac agtgtctgtt taccgtgtct
     3961 cactggattt tatttttaga gtttttcaat atgacttaaa acaagagaaa ttcacacagt
     4021 gggataaata attcaaaaaa ctaaaaaggg aacctggaaa tgaaaccttc agacactcga
     4081 gaagagtaca gttccagttc acgtggagag aggcaggctt tgtttatgtt ggagtcatgt
     4141 ttgttaataa acactaattt taatgctatc attgaggtct tttatagaaa atttttagta
     4201 atcacattca caaataaaac aggattctag ttcttaagta aatcaagatt atatgtatca
     4261 tttcttagaa ataagttaat ttaaaaaggt tatttttaat aggcaattgg cctaggtttt
     4321 tccgaagctg aggtttaaaa aacaaaacaa aacaacagtt tcaccttgaa tcggggaaca
     4381 ttaatatttc taaacatgtg tttgtgtcac gtatcgagta gagttcattt taagagactt
     4441 gatttcccct tcccatgatt tttggttggt agaggctacg tggggtagat gggattggat
     4501 gttttcagtt gtttaaatca ttgtcattaa gtataaaatg caaaattatt tatgtttttg
     4561 aatgaatcat tcatgtcaca aataaaatca gtcattgaga aaccaaaaaa aa
//
