LOCUS       NM_008129               2072 bp    mRNA    linear   ROD 06-NOV-2024
DEFINITION  Mus musculus glutamate-cysteine ligase, modifier subunit (Gclm),
            mRNA.
ACCESSION   NM_008129
VERSION     NM_008129.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Nedomova,M., Haberecht-Muller,S., Moller,S., Venz,S.,
            Prochazkova,M., Prochazka,J., Sedlak,F., Chawengsaksophak,K.,
            Hammer,E., Kasparek,P., Adamek,M., Sedlacek,R., Konvalinka,J.,
            Kruger,E. and Grantz Saskova,K.
  TITLE     DDI2 protease controls embryonic development and inflammation via
            TCF11/NRF1
  JOURNAL   iScience 27 (10), 110893 (2024)
   PUBMED   39328932
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Xue,Z., Nuerrula,Y., Sitiwaerdi,Y. and Eli,M.
  TITLE     Nuclear factor erythroid 2-related factor 2 promotes
            radioresistance by regulating glutamate-cysteine ligase modifier
            subunit and its unique immunoinvasive pattern
  JOURNAL   Biomol Biomed 24 (3), 545-559 (2024)
   PUBMED   38340316
  REMARK    GeneRIF: Nuclear factor erythroid 2-related factor 2 promotes
            radioresistance by regulating glutamate-cysteine ligase modifier
            subunit and its unique immunoinvasive pattern.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2072)
  AUTHORS   Skupienski,R., Steullet,P., Do,K.Q. and Xin,L.
  TITLE     Developmental changes in cerebral NAD and neuroenergetics of an
            antioxidant compromised mouse model of schizophrenia
  JOURNAL   Transl Psychiatry 13 (1), 275 (2023)
   PUBMED   37543592
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2072)
  AUTHORS   Lim,J., Shioda,T., Malott,K.F., Shioda,K., Odajima,J., Leon
            Parada,K.N., Nguyen,J., Getze,S., Lee,M., Nguyen,J., Reshel
            Blakeley,S., Trinh,V., Truong,H.A. and Luderer,U.
  TITLE     Prenatal exposure to benzo[a]pyrene depletes ovarian reserve and
            masculinizes embryonic ovarian germ cell transcriptome
            transgenerationally
  JOURNAL   Sci Rep 13 (1), 8671 (2023)
   PUBMED   37248279
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2072)
  AUTHORS   Chen,Y., Holland,K.D., Shertzer,H.G., Nebert,D.W. and Dalton,T.P.
  TITLE     Fatal Epileptic Seizures in Mice Having Compromised Glutathione and
            Ascorbic Acid Biosynthesis
  JOURNAL   Antioxidants (Basel) 12 (2), 448 (2023)
   PUBMED   36830006
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2072)
  AUTHORS   Thompson,S.A., White,C.C., Krejsa,C.M., Eaton,D.L. and
            Kavanagh,T.J.
  TITLE     Modulation of glutathione and glutamate-L-cysteine ligase by
            methylmercury during mouse development
  JOURNAL   Toxicol Sci 57 (1), 141-146 (2000)
   PUBMED   10966520
REFERENCE   7  (bases 1 to 2072)
  AUTHORS   Hudson,F.N. and Kavanagh,T.J.
  TITLE     Cloning and characterization of the proximal promoter region of the
            mouse glutamate-L-cysteine ligase regulatory subunit gene
  JOURNAL   Biochim Biophys Acta 1492 (2-3), 447-451 (2000)
   PUBMED   10899580
REFERENCE   8  (bases 1 to 2072)
  AUTHORS   Reid,L.L., Botta,D., Shao,J., Hudson,F.N. and Kavanagh,T.J.
  TITLE     Molecular cloning and sequencing of the cDNA encoding mouse
            glutamate-cysteine ligase regulatory subunit
  JOURNAL   Biochim Biophys Acta 1353 (2), 107-110 (1997)
   PUBMED   9294003
REFERENCE   9  (bases 1 to 2072)
  AUTHORS   Gage,P.J. and Camper,S.A.
  TITLE     Pituitary homeobox 2, a novel member of the bicoid-related family
            of homeobox genes, is a potential regulator of anterior structure
            formation
  JOURNAL   Hum Mol Genet 6 (3), 457-464 (1997)
   PUBMED   9147650
REFERENCE   10 (bases 1 to 2072)
  AUTHORS   Tsuchiya,K., Mulcahy,R.T., Reid,L.L., Disteche,C.M. and
            Kavanagh,T.J.
  TITLE     Mapping of the glutamate-cysteine ligase catalytic subunit gene
            (GLCLC) to human chromosome 6p12 and mouse chromosome 9D-E and of
            the regulatory subunit gene (GLCLR) to human chromosome 1p21-p22
            and mouse chromosome 3H1-3
  JOURNAL   Genomics 30 (3), 630-632 (1995)
   PUBMED   8825659
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB615104.1, AK028236.1 and DV659479.1.
            
            On Feb 12, 2013 this sequence version replaced NM_008129.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK172387.1, AK028236.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                BB615104.1         1-35
            36-1505             AK028236.1         2-1471
            1506-2072           DV659479.1         143-709
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 52.94 cM"
     gene            1..2072
                     /gene="Gclm"
                     /gene_synonym="Gcmc; Glclr"
                     /note="glutamate-cysteine ligase, modifier subunit"
                     /db_xref="GeneID:14630"
                     /db_xref="MGI:MGI:104995"
     exon            1..555
                     /gene="Gclm"
                     /gene_synonym="Gcmc; Glclr"
                     /inference="alignment:Splign:2.1.0"
     CDS             430..1254
                     /gene="Gclm"
                     /gene_synonym="Gcmc; Glclr"
                     /EC_number="6.3.2.2"
                     /note="GCS light chain; gamma-ECS regulatory subunit;
                     gamma-glutamylcysteine synthetase regulatory subunit"
                     /codon_start=1
                     /product="glutamate--cysteine ligase regulatory subunit"
                     /protein_id="NP_032155.1"
                     /db_xref="CCDS:CCDS17808.1"
                     /db_xref="GeneID:14630"
                     /db_xref="MGI:MGI:104995"
                     /translation="MGTDSRAAGALLARASTLHLQTGNLLNWGRLRKKCPSTHSEELR
                     DCIQKTLNEWSSQISPDLVREFPDVLECTMSHAVEKINPDEREEMKVSAKLFIVGSNS
                     SSSTRSAVDMACSVLGVAQLDSVIMASPPIEDGVNLSLEHLQPYWEELENLVQSKKIV
                     AIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPK
                     ELLSEASFQEALQESIPDIEAQDWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS"
     exon            556..621
                     /gene="Gclm"
                     /gene_synonym="Gcmc; Glclr"
                     /inference="alignment:Splign:2.1.0"
     exon            622..706
                     /gene="Gclm"
                     /gene_synonym="Gcmc; Glclr"
                     /inference="alignment:Splign:2.1.0"
     exon            707..766
                     /gene="Gclm"
                     /gene_synonym="Gcmc; Glclr"
                     /inference="alignment:Splign:2.1.0"
     exon            767..969
                     /gene="Gclm"
                     /gene_synonym="Gcmc; Glclr"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1084
                     /gene="Gclm"
                     /gene_synonym="Gcmc; Glclr"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..2072
                     /gene="Gclm"
                     /gene_synonym="Gcmc; Glclr"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1768..1773
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gclm"
                     /gene_synonym="Gcmc; Glclr"
                     /note="hexamer: AATAAA"
     polyA_site      1794
                     /gene="Gclm"
                     /gene_synonym="Gcmc; Glclr"
                     /note="major polyA site"
ORIGIN      
        1 aggccgaggg ccagtcaccc gcgggcctgc gccccgcccc tggcccgccg cgggacgagt
       61 aacggttacg aagcactttc tcgggtgagg tttctgctta gtcattgtct tccaggaaac
      121 agctccctcg tttcagcggc ggcggcggtg gcggccgcag aagccgcagc gaccgtccgc
      181 gccggcgcct ccctcccttc cttccctccc tccgggaccg cctctccgtc gccgcccggc
      241 ctcctcgggc ccgagcgcac accgggctgt ggccgcgcgg agtcggcagc ctcgcgctct
      301 cctctggggc ctggggcacc gccgcgccct gcgaggcagc cgtctgccca ctcgcccgcc
      361 cgcctgcccg cccgctcgcc atctctccgc ggctccggcg ctacccggtc ccctccggcg
      421 gcagctgcca tgggcaccga cagccgcgcg gccggggcgc tcctggcgcg ggccagcacc
      481 ctgcacctgc agaccgggaa cctgctcaac tggggccgcc tgcgaaaaaa gtgcccgtcc
      541 acgcacagcg aggagcttcg ggactgtatc caaaagacct tgaatgaatg gagttcccaa
      601 atcagccccg atttagtcag ggagtttcca gatgttttgg aatgcaccat gtcccatgca
      661 gtggagaaga taaatcccga tgaaagagaa gaaatgaaag tttctgcaaa actgttcatt
      721 gtaggatcga attcttcatc atcaactaga agtgcagttg acatggcatg ctccgtcctt
      781 ggagttgcac agctggactc tgtgatcatg gcttcgcctc cgattgaaga tggagttaat
      841 ctttccttgg agcatttaca gccttactgg gaggaattag agaacttagt tcagagcaag
      901 aaaattgttg ctataggcac ctctgatcta gacaagacac agttggagca gctgtatcag
      961 tgggcacagg taaaacccaa tagtaaccaa gttaacctgg cctcctgctg tgtgatgcca
     1021 ccagatttga ctgcctttgc taaacaattt gacatacagc tactgactca caatgacccg
     1081 aaagaactgc tctctgaggc aagtttccag gaagctcttc aagaaagcat ccctgacatt
     1141 gaagcccagg attgggtgcc gctgtggctg ctgaggtact cggtcatcgt gaagagcagg
     1201 ggaatcatca agtctaaagg ctacattctg caagccaaaa ggaggggctc ttaactccag
     1261 ctcacaccca cagctcaccg acctgctgtt gtctggaaca taagatacaa attcatgata
     1321 acattgagat gtgtttaaaa aaaaaaaaat atagctccgc cttacaagaa gcatccccga
     1381 ggtgcgagtg cagttgttcg gcactgtgtt gagttttgag ggcctcccgt gcctctgcag
     1441 cagcgcgctc acaggaacca ctgtattcta atcattaagc tgttgttttc taacggaaat
     1501 cgtctataaa gaaaaatact tgcaatatat ttcctttatt tttatgagca atagaaatca
     1561 agaaaatttg ttttaagata tattttggct taggcatcag ggtgatgtat atacatattt
     1621 tttatttcca aaattcatta attgcttcct attctatact tctataagcg attacattac
     1681 agttgttaaa acatactaga tttttttttc ccctgtcatt ggcaaaataa tctatctcag
     1741 taactatagc agtctaccag taatgtaaat aaaattcatt ctttgatcca cgaatggaaa
     1801 tttaatgtgt gtgttcttgc ttacagcgta gctatagcta tgcattgacc acatgttctg
     1861 ggtcatgttt cttccacctc ttatctacaa aggcagagtg tctaagaagg cggcttgatg
     1921 ctttaacatt attgtttggg ggtttcataa aagcaactac tgttgctatc ttcagagtct
     1981 ttggacctct tcctgtactc tcagtgtgtt gctgctcagt tggactcacc acatgcttag
     2041 cactatgggc tctggagaga tgaactaaga at
//
