LOCUS       NM_010295               3442 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus glutamate-cysteine ligase, catalytic subunit (Gclc),
            transcript variant 1, mRNA.
ACCESSION   NM_010295
VERSION     NM_010295.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Bonetti,L., Horkova,V., Grusdat,M., Longworth,J., Guerra,L.,
            Kurniawan,H., Franchina,D.G., Soriano-Baguet,L., Binsfeld,C.,
            Verschueren,C., Spath,S., Ewen,A., Koncina,E., Gerardy,J.J.,
            Kobayashi,T., Dostert,C., Farinelle,S., Harm,J., Fan,Y.T., Chen,Y.,
            Harris,I.S., Lang,P.A., Vasiliou,V., Waisman,A., Letellier,E.,
            Becher,B., Mittelbronn,M. and Brenner,D.
  TITLE     A Th17 cell-intrinsic glutathione/mitochondrial-IL-22 axis protects
            against intestinal inflammation
  JOURNAL   Cell Metab 36 (8), 1726-1744 (2024)
   PUBMED   38986617
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Asantewaa,G., Tuttle,E.T., Ward,N.P., Kang,Y.P., Kim,Y.,
            Kavanagh,M.E., Girnius,N., Chen,Y., Rodriguez,K., Hecht,F.,
            Zocchi,M., Smorodintsev-Schiller,L., Scales,T.Q., Taylor,K.,
            Alimohammadi,F., Duncan,R.P., Sechrist,Z.R., Agostini-Vulaj,D.,
            Schafer,X.L., Chang,H., Smith,Z.R., O'Connor,T.N., Whelan,S.,
            Selfors,L.M., Crowdis,J., Gray,G.K., Bronson,R.T., Brenner,D.,
            Rufini,A., Dirksen,R.T., Hezel,A.F., Huber,A.R., Munger,J.,
            Cravatt,B.F., Vasiliou,V., Cole,C.L., DeNicola,G.M. and Harris,I.S.
  TITLE     Glutathione synthesis in the mouse liver supports lipid abundance
            through NRF2 repression
  JOURNAL   Nat Commun 15 (1), 6152 (2024)
   PUBMED   39034312
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3442)
  AUTHORS   Hashim,K.N.B., Matsuba,Y., Takahashi,M., Kamano,N., Tooyama,I.,
            Saido,T.C. and Hashimoto,S.
  TITLE     Neuronal glutathione depletion elevates the Abeta42/Abeta40 ratio
            and tau aggregation in Alzheimer's disease mice
  JOURNAL   FEBS Lett 598 (13), 1576-1590 (2024)
   PUBMED   38789405
REFERENCE   4  (bases 1 to 3442)
  AUTHORS   Wei,Z., Hao,C., Radeen,K.R., Srinivasagan,R., Chen,J.K., Sharma,S.,
            McGee-Lawrence,M.E., Hamrick,M.W., Monnier,V.M. and Fan,X.
  TITLE     Prevention of age-related truncation of gamma-glutamylcysteine
            ligase catalytic subunit (GCLC) delays cataract formation
  JOURNAL   Sci Adv 10 (17), eadl1088 (2024)
   PUBMED   38669339
  REMARK    GeneRIF: Prevention of age-related truncation of
            gamma-glutamylcysteine ligase catalytic subunit (GCLC) delays
            cataract formation.
REFERENCE   5  (bases 1 to 3442)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   6  (bases 1 to 3442)
  AUTHORS   El Mouatassim,S., Guerin,P. and Menezo,Y.
  TITLE     Expression of genes encoding antioxidant enzymes in human and mouse
            oocytes during the final stages of maturation
  JOURNAL   Mol Hum Reprod 5 (8), 720-725 (1999)
   PUBMED   10421798
REFERENCE   7  (bases 1 to 3442)
  AUTHORS   Reid,L.L., Botta,D., Lu,Y., Gallagher,E.P. and Kavanagh,T.J.
  TITLE     Molecular cloning and sequencing of the cDNA encoding the catalytic
            subunit of mouse glutamate-cysteine ligase
  JOURNAL   Biochim Biophys Acta 1352 (3), 233-237 (1997)
   PUBMED   9224945
REFERENCE   8  (bases 1 to 3442)
  AUTHORS   Kang,Y., Oiao,X., Jurma,O., Knusel,B. and Andersen,J.K.
  TITLE     Cloning/brain localization of mouse glutamylcysteine synthetase
            heavy chain mRNA
  JOURNAL   Neuroreport 8 (8), 2053-2060 (1997)
   PUBMED   9223101
REFERENCE   9  (bases 1 to 3442)
  AUTHORS   Tsuchiya,K., Mulcahy,R.T., Reid,L.L., Disteche,C.M. and
            Kavanagh,T.J.
  TITLE     Mapping of the glutamate-cysteine ligase catalytic subunit gene
            (GLCLC) to human chromosome 6p12 and mouse chromosome 9D-E and of
            the regulatory subunit gene (GLCLR) to human chromosome 1p21-p22
            and mouse chromosome 3H1-3
  JOURNAL   Genomics 30 (3), 630-632 (1995)
   PUBMED   8825659
REFERENCE   10 (bases 1 to 3442)
  AUTHORS   Harvey,M.B., Arcellana-Panlilio,M.Y., Zhang,X., Schultz,G.A. and
            Watson,A.J.
  TITLE     Expression of genes encoding antioxidant enzymes in preimplantation
            mouse and cow embryos and primary bovine oviduct cultures employed
            for embryo coculture
  JOURNAL   Biol Reprod 53 (3), 532-540 (1995)
   PUBMED   7578676
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK144339.1, AK160876.1 and EH103060.1.
            
            On Mar 1, 2011 this sequence version replaced NM_010295.1.
            
            Transcript Variant: This variant (1) represents the protein-coding
            transcript.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660819.26438.1, AK160876.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-241               AK144339.1         2-242
            242-3295            AK160876.1         2-3055
            3296-3442           EH103060.1         31-177              c
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 43.36 cM"
     gene            1..3442
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /note="glutamate-cysteine ligase, catalytic subunit"
                     /db_xref="GeneID:14629"
                     /db_xref="MGI:MGI:104990"
     exon            1..425
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /inference="alignment:Splign:2.1.0"
     CDS             276..2189
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /EC_number="6.3.2.2"
                     /note="gamma-glutamylcysteine synthetase heavy subunit;
                     gamma-ECS; GCS heavy chain; gamma GCS-HS"
                     /codon_start=1
                     /product="glutamate--cysteine ligase catalytic subunit"
                     /protein_id="NP_034425.1"
                     /db_xref="CCDS:CCDS23354.1"
                     /db_xref="GeneID:14629"
                     /db_xref="MGI:MGI:104990"
                     /translation="MGLLSQGSPLSWEETQRHADHVRRHGILQFLHIYHAVKDRHKDV
                     LKWGDEVEYMLVSFDHENRKVQLLLNGGDVLETLQEKGERTNPNHPTLWRPEYGSYMI
                     EGTPGQPYGGTMSEFNTVEANMRKRRKEATSVLGEHQALCTITSFPRLGCPGFTLPEH
                     RPNPEEGGASKSLFFPDEAINKHPRFGTLTRNIRHRRGEKVVINVPIFKDKNTPSPFV
                     ETFPEDAEASKASQPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIV
                     MALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNRFRISKSRYDSIDS
                     YLSKCGEKYNDIDLTIDKEIYEQLLEEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDA
                     NESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTR
                     VILSYKLDFLIPLSKVDENMKVAQKRDAVLQGMFYFRKDICKGGNAVVDGCSKAQSSS
                     EPAAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRA
                     SGELMTVARWMREFIANHPDYKQDSVITDEINYSLIWKCNQIADELCECPELLGSGFR
                     KAKYSGGKSDPSA"
     exon            426..538
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            539..721
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            722..835
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            836..894
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            895..1028
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1103
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1220
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1359
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1472
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..1565
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1670
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            1671..1752
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..1856
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            1857..1977
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            1978..3427
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3262..3267
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /note="hexamer: AATAAA"
     polyA_site      3296
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /note="major polyA site"
     regulatory      3398..3403
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
                     /note="hexamer: AATAAA"
     polyA_site      3423
                     /gene="Gclc"
                     /gene_synonym="D9Wsu168e; Ggcs-hs; GLCL-H; Glclc"
ORIGIN      
        1 aggcgcggaa ctgggcatgc tctgtgtcgc cgcagctttg ggtcgcaagt aggaagcccg
       61 tgcacgacac cggcggcgcc gagtggagcg ctgccgcggc ggccagggcc gcagggccgg
      121 cgtgggagcg cgagaccggg cggcggcgag agaccccgac ccgggctgtc aaccgcgcgg
      181 cggggtccga gccggagcgg gaacggacgg gacgcccgcc gcgcggcccg agggcgcccc
      241 cagagcggag ctgcggccgg gaggaggacg cggccatggg gctgctgtcc caaggctcgc
      301 cactgagctg ggaagagacc cagcgccacg ccgaccacgt gcggaggcac ggcatcctcc
      361 agttcctgca catctaccac gcagtcaagg accggcacaa ggacgtgctc aagtggggtg
      421 acgaggtgga gtacatgttg gtgtccttcg atcatgaaaa taggaaagtc cagttgttac
      481 tgaatggagg cgatgttctt gagactctgc aggagaaggg ggagaggaca aaccccaacc
      541 atccgaccct ctggagacca gagtatggga gttacatgat cgaaggaacg cctggccagc
      601 cctacggagg aacgatgtct gagttcaaca ctgtggaggc caatatgagg aaacgccgga
      661 aggaggctac ttctgtacta ggagagcatc aggctctctg caccatcact tcattcccca
      721 gactaggctg tcctggattc acactgccag aacacagacc caacccagag gaaggaggcg
      781 catcaaagtc cctcttcttt ccagatgaag ccataaacaa gcacccccgc ttcggtactc
      841 taacaagaaa catccggcat cggagaggag aaaaggttgt catcaatgtg ccaatattca
      901 aggacaagaa tacaccatct ccatttgtag aaacatttcc agaagatgcg gaggcatcaa
      961 aggcttctca gccagaccat atctacatgg atgccatggg attcgggatg ggcaactgct
     1021 gtctccaggt gacattccaa gcctgcagca tatctgaggc aagatacctt tatgatcagc
     1081 tggccactat ctgcccaatt gttatggctt tgagtgctgc atctccattt taccgaggct
     1141 acgtgtcaga cattgattgt cgctggggag tgatttctgc ctctgtagat gatagaacac
     1201 gggaggagag aggcctggag cctctgaaga acaatcgctt taggatcagt aagtctcggt
     1261 atgactcaat agatagctac ctgtctaagt gtggggagaa gtacaatgac atcgacctga
     1321 ccatcgataa ggagatctac gagcagctcc tggaggaagg catcgatcat cttctggcac
     1381 agcacgttgc tcatctcttt attagagacc cactcaccct ttttgaagag aaaatacacc
     1441 tggatgatgc caacgagtct gaccattttg agaatattca gtctacaaac tggcagacaa
     1501 tgaggtttaa gcctcctcct ccaaactcag atattggatg gagagtagag ttccgaccaa
     1561 tggaggtgca gttaacagac tttgagaact ctgcctatgt ggtattcgtg gtactgctca
     1621 ctagggtgat cctctcatac aaactagact tcctcattcc gctgtccaag gttgacgaga
     1681 acatgaaagt ggcccagaag cgagatgctg tcttgcaggg gatgttttat ttcaggaaag
     1741 acatctgcaa aggcggcaac gctgtggtgg acgggtgcag caaggcccag agcagctctg
     1801 agccagctgc agaggagtac accctgatga gcatagacac catcatcaat gggaaggaag
     1861 gggtatttcc tggactcatc cccattctga actcctatct ggagaacatg gaagttgatg
     1921 tggacacccg atgcagtatt ctgaactacc taaagctaat taagaagaga gcatctggag
     1981 aactaatgac tgttgccagg tggatgagag agttcattgc aaaccatcct gactacaagc
     2041 aagacagtgt aataactgat gaaatcaact atagcctcat ttggaagtgc aaccaaattg
     2101 cagatgagct gtgtgaatgt ccagagttac ttggatcagg atttagaaaa gcgaagtaca
     2161 gtggaggtaa aagcgaccct tcagcctagc cttcagccag cagagagaga gagggtgatc
     2221 gctgtttagg atggcgtgtg cagccctgac tcctggcccg ctgggacgct gggaagacca
     2281 agatgacctg cagtgtttct gcgccagttg cctgcaagtg gctactagaa gctcccttca
     2341 gtaggtaaat ctgagtttgt acatgtatat actaaagtat ttttatgatt aacagtgtat
     2401 tttaataaca ttgtatctaa agtcatagtg aactggcttg tacattttga attcttactc
     2461 tggagcaact actgtctaag cagttttgta aatgtaattg tacaataata ccggcattcc
     2521 agagtttcaa atgtttactg taaatcttcc tttaatacta cctgggacct gattcactga
     2581 aatttactta aaaaaaaaaa aaaggctttc tcacacctgt ttcttgagtc tcctcttact
     2641 atgtaggtaa agtcactgga ctgcattgat gccctccgtg ggtgaggcac cttacctgtg
     2701 atatttcctt tgcaaagcag ccccactctc ttgccactga attctgctag ctcttgaaaa
     2761 gaaactactt taggaattgt ctaaatatac agactcagag aatatttcat ctgaaaagga
     2821 aaatcacttc cttctacata cactgagagc tttgtcaaat ggtgtccgct cttccattac
     2881 cacctgctgt ctgacccctg tgctgatgaa taatcaactc agtgcagcag ccacggtttc
     2941 ctgcccttct ctagtttgca ggagcagatt gacaggctgt cttctaacag ccattcattt
     3001 cattccagac tttccccagg gagtagtctc cacccgcttg ctttctctat aaaactcaca
     3061 aatcaatcat gaaatgcact aatttttgtg aatcaggacc ctaaatgttt aattgtaaat
     3121 aatttgtttc atagattgct tttagctttg tgttatcttt ttaattgtta actaaattta
     3181 cttctaaaat aactactgta gattaagcca agctttacag atataaatat ttacactgta
     3241 atggtaattt tgaaagattg caataaagcc gcttcatctt aaagctttca atactagaat
     3301 tggctctata tctcatttac ttcagaaagt atttttttct acagttctct tatgtaaatt
     3361 aggttgtaaa aagaataaaa tgtttgtggt ctgagaaaat aaaaaatggg attcctattc
     3421 aaaggtaaaa aaaaaaaaaa aa
//
