LOCUS       NM_001374741            2140 bp    mRNA    linear   ROD 07-DEC-2024
DEFINITION  Mus musculus glucokinase regulatory protein (Gckr), transcript
            variant 1, mRNA.
ACCESSION   NM_001374741 XM_006503881
VERSION     NM_001374741.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ford,B.E., Chachra,S.S., Alshawi,A., Oakley,F., Fairclough,R.J.,
            Smith,D.M., Tiniakos,D. and Agius,L.
  TITLE     Compromised chronic efficacy of a glucokinase activator AZD1656 in
            mouse models for common human GCKR variants
  JOURNAL   Biochem Pharmacol 229, 116499 (2024)
   PUBMED   39173844
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Buziau,A.M., Oosterveer,M.H., Wouters,K., Bos,T., Tolan,D.R.,
            Agius,L., Ford,B.E., Cassiman,D., Stehouwer,C.D.A., Schalkwijk,C.G.
            and Brouwers,M.C.G.J.
  TITLE     Hepatic glucokinase regulatory protein and carbohydrate response
            element binding protein attenuation reduce de novo lipogenesis but
            do not mitigate intrahepatic triglyceride accumulation in Aldob
            deficiency
  JOURNAL   Mol Metab 87, 101984 (2024)
   PUBMED   38972375
  REMARK    GeneRIF: Hepatic glucokinase regulatory protein and carbohydrate
            response element binding protein attenuation reduce de novo
            lipogenesis but do not mitigate intrahepatic triglyceride
            accumulation in Aldob deficiency.
REFERENCE   3  (bases 1 to 2140)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2140)
  AUTHORS   Ford,B.E., Chachra,S.S., Rodgers,K., Moonira,T., Al-Oanzi,Z.H.,
            Anstee,Q.M., Reeves,H.L., Schattenberg,J.M., Fairclough,R.J.,
            Smith,D.M., Tiniakos,D. and Agius,L.
  TITLE     The GCKR-P446L gene variant predisposes to raised blood cholesterol
            and lower blood glucose in the P446L mouse-a model for GCKR
            rs1260326
  JOURNAL   Mol Metab 72, 101722 (2023)
   PUBMED   37031802
  REMARK    GeneRIF: The GCKR-P446L gene variant predisposes to raised blood
            cholesterol and lower blood glucose in the P446L mouse-a model for
            GCKR rs1260326.
REFERENCE   5  (bases 1 to 2140)
  AUTHORS   Codner,G.F., Mianne,J., Caulder,A., Loeffler,J., Fell,R., King,R.,
            Allan,A.J., Mackenzie,M., Pike,F.J., McCabe,C.V., Christou,S.,
            Joynson,S., Hutchison,M., Stewart,M.E., Kumar,S., Simon,M.M.,
            Agius,L., Anstee,Q.M., Volynski,K.E., Kullmann,D.M., Wells,S. and
            Teboul,L.
  TITLE     Application of long single-stranded DNA donors in genome editing:
            generation and validation of mouse mutants
  JOURNAL   BMC Biol 16 (1), 70 (2018)
   PUBMED   29925374
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2140)
  AUTHORS   Kaminski,M.T., Schultz,J., Waterstradt,R., Tiedge,M., Lenzen,S. and
            Baltrusch,S.
  TITLE     Glucose-induced dissociation of glucokinase from its regulatory
            protein in the nucleus of hepatocytes prior to nuclear export
  JOURNAL   Biochim Biophys Acta 1843 (3), 554-564 (2014)
   PUBMED   24333860
REFERENCE   7  (bases 1 to 2140)
  AUTHORS   Grimsby,J., Coffey,J.W., Dvorozniak,M.T., Magram,J., Li,G.,
            Matschinsky,F.M., Shiota,C., Kaur,S., Magnuson,M.A. and Grippo,J.F.
  TITLE     Characterization of glucokinase regulatory protein-deficient mice
  JOURNAL   J Biol Chem 275 (11), 7826-7831 (2000)
   PUBMED   10713097
REFERENCE   8  (bases 1 to 2140)
  AUTHORS   Weninger,S.C., Peters,L.L. and Majzoub,J.A.
  TITLE     Urocortin expression in the Edinger-Westphal nucleus is
            up-regulated by stress and corticotropin-releasing hormone
            deficiency
  JOURNAL   Endocrinology 141 (1), 256-263 (2000)
   PUBMED   10614646
REFERENCE   9  (bases 1 to 2140)
  AUTHORS   Farrelly,D., Brown,K.S., Tieman,A., Ren,J., Lira,S.A., Hagan,D.,
            Gregg,R., Mookhtiar,K.A. and Hariharan,N.
  TITLE     Mice mutant for glucokinase regulatory protein exhibit decreased
            liver glucokinase: a sequestration mechanism in metabolic
            regulation
  JOURNAL   Proc Natl Acad Sci U S A 96 (25), 14511-14516 (1999)
   PUBMED   10588736
REFERENCE   10 (bases 1 to 2140)
  AUTHORS   Wightman,P.J., Hayward,B.E. and Bonthron,D.T.
  TITLE     The genes encoding glucokinase regulatory protein and
            ketohexokinase co-localize to mouse chromosome 5
  JOURNAL   Mamm Genome 8 (9), 700-701 (1997)
   PUBMED   9271679
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC114619.9.
            
            On Oct 10, 2019 this sequence version replaced XM_006503881.4.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17784646.763218.1,
                                           SRR17784649.1178994.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849386, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-117               AC114619.9         30664-30780         c
            118-273             AC114619.9         30023-30178         c
            274-342             AC114619.9         29561-29629         c
            343-411             AC114619.9         28717-28785         c
            412-485             AC114619.9         28271-28344         c
            486-552             AC114619.9         27698-27764         c
            553-606             AC114619.9         27478-27531         c
            607-701             AC114619.9         26092-26186         c
            702-807             AC114619.9         23269-23374         c
            808-926             AC114619.9         21737-21855         c
            927-1025            AC114619.9         21179-21277         c
            1026-1123           AC114619.9         20925-21022         c
            1124-1200           AC114619.9         20512-20588         c
            1201-1297           AC114619.9         20062-20158         c
            1298-1395           AC114619.9         19410-19507         c
            1396-1479           AC114619.9         19239-19322         c
            1480-1629           AC114619.9         3622-3771           c
            1630-1764           AC114619.9         1852-1986           c
            1765-2140           AC114619.9         1041-1416           c
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 17.27 cM"
     gene            1..2140
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /note="glucokinase regulatory protein"
                     /db_xref="GeneID:231103"
                     /db_xref="MGI:MGI:1096345"
     exon            1..117
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     exon            118..273
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     exon            274..342
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     exon            343..411
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     exon            412..485
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     exon            486..552
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     exon            553..606
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     exon            607..701
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     exon            702..807
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     exon            808..926
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1025
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1123
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1200
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1297
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1395
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1396..1479
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1480..1629
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1630..1764
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1765..2140
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2116..2121
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /note="hexamer: ACTAAA"
     polyA_site      2140
                     /gene="Gckr"
                     /gene_synonym="GKRP"
                     /note="major polyA site"
ORIGIN      
        1 agaacagatt gcgtgagtgc agaggcaggc gggttggaga gtctgcagtg tgggaccatg
       61 ccaagcacca agcggtatca gcatgtgatc gagacccctg agcctgggga atgggagttg
      121 tcagggtatg aagcagctgt gccaatcaca gagaagtcca acccactgac ccggaacttg
      181 gacaaagcag atgcagagaa aattgttcaa ctgctggggc agtgtgatgc tgagatattc
      241 caggaggagg ggcaaatcat gcccacctac cagcgactgt acagtgagtc agttctgacc
      301 accatgttgc aagtggctgg caaggtccag gaagtgctga aggagccaga tgggggcctg
      361 gtggtgctga gtggaggggg cacctctggt cgtatggcat tccttatgtc tgtgtctttc
      421 aaccagctga tgaaaggtct gggacaaaaa cctctttaca catacctcat tgcagggggt
      481 gacaggtctg ttgtagcctc tcgggaacgg acagaagata gcgccctaca cggaatcgag
      541 gagctgaaga aggtggctgc tgggaaaaag agagtggtcg ttataggcat ttccgtggga
      601 ctctctgcgc cctttgtggc aggccagatg gactactgca tggataacac agctgtcttc
      661 ttgccggtcc tggttggctt caatccggtg agcatggcca gaaatgatcc cattgaagac
      721 tggagatcga cattccgaca agtggcagag cggatgcaga agatgcagga gaaacaggaa
      781 gcctttgtgc tcaatcctgc catcgggcct gaggggctca gtggctcttc ccgaatgaaa
      841 ggtggaagcg ccaccaagat tctactggaa accctgctac tagcagccca taagactgtg
      901 gaccagggtg ttgtgtcctc tcaaagatgc cttctggaaa tcctgaggac atttgagcgg
      961 gctcatcagg taacctacag tcaaagttcc aaaattgcca ctctgacgaa gcaagttggc
     1021 atcagcctgg agaaaaaagg ccacgtgcac ttggttggct ggcagaccct cggtatcatc
     1081 gccattatgg atggggtaga gtgtatccac acttttggtg ctgatttccg agatatccgt
     1141 ggctttctta ttggtgacca caatgacatg tttaaccaga aggatgagct cagcaatcag
     1201 ggtccccagt tcaccttctc tcaggatgac ttcctgactt ctgttctgcc atcccttacg
     1261 gaaattgaca ctgtggtctt catttttacc ctggatgata acctcgcaga agtacaggcc
     1321 ctggcagaaa gggtgaggga gaagagttgg aacatccagg ccctggtgca cagcacagtg
     1381 gggcagtcct tgccagctcc tctaaagaag ctctttccct cgctcatcag catcacatgg
     1441 ccacttcttt tcttcgatta tgaagggagc tacgttcaga agttccagcg tgagttaagc
     1501 accaagtggg tgttgaatac agtgagtact ggggcccatg tgctgctggg gaagatccta
     1561 cagaaccaca tgctggacct ccgcatcgcc aactccaaac tcttctggag ggcactggcc
     1621 atgttgcaga ggttctcagg acagtccaag gctcgctgca ttgagagtct tcttcaagtg
     1681 atacatttcc ctcaaccgct gtcgaatgat gtccgcgcgg cccccatctc ctgccatgtc
     1741 caggttgccc acgagaagga aaaggtgatc cccacagcct tgctgagtct cctactcagg
     1801 tgctccatca ctgaggctaa ggaacgcctg gctgcagctt cttcagtctg tgaggttgtt
     1861 aggagcgccc tctctgggcc aggtcagaaa cgcagcatcc aagcctttgg agaccctgtg
     1921 gtgccctgaa ttgatgtttc tgtgaccatt gaaaagtctc catccacttc tgaggggact
     1981 tgtgccagta atacacgttg caggacagac tcagttccgg tgggtggggc actaacagcc
     2041 cagaattgag gaagagtcct gttctcagct ggatcatttc catttttact ggcatcttct
     2101 gatcttagga ataaaactaa atgtcttgtt ttgaacccaa
//
