LOCUS       NM_010292               2727 bp    mRNA    linear   ROD 18-MAR-2025
DEFINITION  Mus musculus glucokinase (Gck), transcript variant 1, mRNA.
ACCESSION   NM_010292
VERSION     NM_010292.5
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Waite,E.L., Tigue,M., Yu,M., Lahori,D., Kelly,K., May,C.L.,
            Naji,A., Roman,J., Doliba,N., Avrahami,D., Nguyen-Ngoc,K.V.,
            Sander,M., Glaser,B. and Kaestner,K.H.
  TITLE     The IsletTester Mouse: An Immunodeficient Model With Stable
            Hyperglycemia for the Study of Human Islets
  JOURNAL   Diabetes 74 (3), 332-342 (2025)
   PUBMED   39571094
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Yang,J., Zhu,L., Pan,H., Ueharu,H., Toda,M., Yang,Q., Hallett,S.A.,
            Olson,L.E. and Mishina,Y.
  TITLE     A BMP-controlled metabolic/epigenetic signaling cascade directs
            midfacial morphogenesis
  JOURNAL   J Clin Invest 134 (8), e165787 (2024)
   PUBMED   38466355
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2727)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2727)
  AUTHORS   Liu,B., Hua,D., Shen,L., Li,T., Tao,Z., Fu,C., Tang,Z., Yang,J.,
            Zhang,L., Nie,A., Jiang,Y., Wang,J., Li,Y., Gu,Y. and Ning,G.
  TITLE     NPC1 is required for postnatal islet beta cell differentiation by
            maintaining mitochondria turnover
  JOURNAL   Theranostics 14 (5), 2058-2074 (2024)
   PUBMED   38505613
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2727)
  AUTHORS   Martinez-Garza,U., Choi,J., Scafidi,S. and Wolfgang,M.J.
  TITLE     Proteomics identifies the developmental regulation of HKDC1 in
            liver of pigs and mice
  JOURNAL   Am J Physiol Regul Integr Comp Physiol 325 (4), R389-R400 (2023)
   PUBMED   37545422
REFERENCE   6  (bases 1 to 2727)
  AUTHORS   Efrat,S., Leiser,M., Wu,Y.J., Fusco-DeMane,D., Emran,O.A.,
            Surana,M., Jetton,T.L., Magnuson,M.A., Weir,G. and Fleischer,N.
  TITLE     Ribozyme-mediated attenuation of pancreatic beta-cell glucokinase
            expression in transgenic mice results in impaired glucose-induced
            insulin secretion
  JOURNAL   Proc Natl Acad Sci U S A 91 (6), 2051-2055 (1994)
   PUBMED   7510884
REFERENCE   7  (bases 1 to 2727)
  AUTHORS   Hughes,S.D., Quaade,C., Milburn,J.L., Cassidy,L. and Newgard,C.B.
  TITLE     Expression of normal and novel glucokinase mRNAs in anterior
            pituitary and islet cells
  JOURNAL   J Biol Chem 266 (7), 4521-4530 (1991)
   PUBMED   1999433
REFERENCE   8  (bases 1 to 2727)
  AUTHORS   James,P.A., Lavender,F.L., Lawrence,G.M. and Walker,D.G.
  TITLE     Comparison of glucokinase in C3H/He and C58 mice that differ in
            their hepatic activity
  JOURNAL   Biochem Genet 23 (7-8), 525-538 (1985)
   PUBMED   3931624
REFERENCE   9  (bases 1 to 2727)
  AUTHORS   Lavender,F.L., James,P.A. and Walker,D.G.
  TITLE     Hepatic glucokinase activity and circulating insulin concentrations
            in two inbred mouse strains
  JOURNAL   Diabetologia 25 (2), 114-119 (1983)
   PUBMED   6354813
REFERENCE   10 (bases 1 to 2727)
  AUTHORS   Coleman,D.L.
  TITLE     Genetic control of glucokinase activity in mice
  JOURNAL   Biochem Genet 15 (3-4), 297-305 (1977)
   PUBMED   141272
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL645469.18.
            
            On Dec 13, 2013 this sequence version replaced NM_010292.4.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the isoform expressed in pancreatic islet
            beta cells.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: L38990.1, SRR1660821.87594.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-527               AL645469.18        61518-62044         c
            528-690             AL645469.18        22825-22987         c
            691-845             AL645469.18        22241-22395         c
            846-965             AL645469.18        21046-21165         c
            966-1061            AL645469.18        18646-18741         c
            1062-1161           AL645469.18        18440-18539         c
            1162-1345           AL645469.18        16324-16507         c
            1346-1501           AL645469.18        15063-15218         c
            1502-1735           AL645469.18        14048-14281         c
            1736-2727           AL645469.18        12786-13777         c
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 3.88 cM"
     gene            1..2727
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /note="glucokinase"
                     /db_xref="GeneID:103988"
                     /db_xref="MGI:MGI:1270854"
     exon            1..527
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     CDS             483..1880
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /EC_number="2.7.1.1"
                     /note="isoform 1 is encoded by transcript variant 1; HK
                     IV; hexokinase-4; hexokinase-D; hexokinase type IV"
                     /codon_start=1
                     /product="hexokinase-4 isoform 1"
                     /protein_id="NP_034422.2"
                     /db_xref="CCDS:CCDS24409.1"
                     /db_xref="GeneID:103988"
                     /db_xref="MGI:MGI:1270854"
                     /translation="MLDDRARMEATKKEKVEQILAEFQLQEEDLKKVMSRMQKEMDRG
                     LKLETHQEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEAGQWSVK
                     TKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDID
                     KGILLNWTKGFKASGAEGNNIVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDR
                     QCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGNSGELDEFLLEYDRM
                     VDESSVNPGQQLYEKIIGGKYMGELVRLVLLKLVEENLLFHGEASEQLRTRGAFETRF
                     VSQVESDSGDRRQILNILSTLGLRPSVADCDIVRRACESVSTRAAHMCSAGLAGVINR
                     MRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPNCEITFIESEEGSGRGAA
                     LVSAVACKKACMLGQ"
     exon            528..690
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     exon            691..845
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     exon            846..965
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1061
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..1161
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1345
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1501
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1735
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     exon            1736..2727
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 caggagcaca gaggccctga caggagacat ctactccaca cctggttgga acagaagcac
       61 cgactgtgac tgagcccaga gaagaaagct gaggcgtgag ggacagagag ttacctgttg
      121 cctcattact caaaagccat ccccaagcca ctggagggag agaccttttg tgctgagtcc
      181 gtctagaggc caccagttcc tcacagctca gcagagctgg aagaaagtca gtcaacactg
      241 aggaaccaca tggctcctcc tgaagaccgc tgggcctgaa gaggccttgg tggggagggg
      301 tccagaagtg aacaatgaaa aagaggaagc tgtggcttca accagcctga ggtggacggc
      361 agagctctct gaggtccggg gctggctgtg actctgtggg ggaagtctgg gctacttctg
      421 ctttggaaag ctgctgcgga acactgaggg gtcccagctc acctgggctg gcggctgggc
      481 agatgctgga tgacagagcc aggatggagg ccaccaagaa ggaaaaggta gagcagatcc
      541 tggcagagtt ccagctgcag gaggaagacc tgaagaaggt gatgagccgg atgcagaagg
      601 agatggaccg tggcctgaag ctggagaccc atcaggaggc cagtgtaaag atgttgccca
      661 cctacgtgcg ttccacccca gaaggctcag aagttggaga ctttctctcc ttagacctgg
      721 gaggaaccaa cttcagggtg atgctggtga aagtggggga gggggaggca ggacagtgga
      781 gcgtgaagac gaaacaccag atgtattcca tccccgagga cgccatgacg ggcactgcgg
      841 agatgctctt tgactacatc tctgagtgca tctctgactt cctggacaag catcagatga
      901 aacacaagaa actacccctg ggcttcacct tctccttccc tgtaaggcac gaagacatag
      961 acaagggcat cctgctcaac tggaccaagg gcttcaaggc ctccggagca gaagggaaca
     1021 acatcgtggg acttctccga gatgctatca agaggagagg ggactttgag atggatgtgg
     1081 tggcaatggt gaatgacacg gtggccacaa tgatctcctg ctactatgaa gaccgccaat
     1141 gtgaggtcgg catgattgtg ggcaccggct gcaacgcctg ctacatggag gagatgcaga
     1201 atgtggagct ggtggaaggc gatgaggggc gcatgtgtgt caacacagag tggggcgcct
     1261 tcgggaactc cggtgagctg gacgagttcc tcctggagta cgaccggatg gtggatgaga
     1321 gctcagtgaa ccccggtcag cagctgtacg aaaagatcat tggcggaaag tacatgggcg
     1381 agctggtacg acttgtgctg ctcaagctgg tagaggagaa tcttctgttc cacggagagg
     1441 cctcagagca gctgcgcaca cgtggtgctt ttgagacccg ttttgtgtcg caggtggaga
     1501 gcgactctgg ggaccgaagg cagatcctta acatcctgag cactctgggc cttcgaccct
     1561 ctgtcgccga ctgcgacatt gtgcgccgtg cctgtgaaag cgtgtccact cgcgccgccc
     1621 acatgtgctc agcaggacta gcgggggtca taaatcgcat gcgcgaaagc cgcagtgagg
     1681 acgtgatgcg catcacggtg ggcgtggatg gctccgtgta caagctgcac ccgagcttca
     1741 aggagcggtt tcacgccagt gtgcgcaggc tgacacccaa ctgcgaaatc accttcattg
     1801 aatcagagga gggcagcggc aggggagccg cactggtctc tgcggtggcc tgcaagaagg
     1861 cttgcatgct gggccagtga aatccaggca aggacaggga cctgggttcc acggggactc
     1921 cacaccccac aaatgctccc agtccactag ggcaggagac ctattctgct gctacccctg
     1981 gaaaatgggg agaggcccct gcaagccaag tcagccagtg agacagccct agggctctca
     2041 gcctggggca aggggcaaga ggatcagcgg caccaaaagc ctttcttgct agaatcaact
     2101 acagagaaag gtgaagcaca ctcaggtctt gctctttcag cttctggcct cccacagctg
     2161 tgggtctggc ctcccaaggg agtgcctcct ggacttgcaa tggcctggct tccctgggaa
     2221 cacatctcca tggggaggta gcttcagcag cttggccaga ccagacctgg gcccccagaa
     2281 gagtaagggc tgcccagacc tggctgtttt cttgcctgtg gctgaagagg ctgcaaaacc
     2341 atgggagacc gactatctag ctacatggag gggactttcc aggccacaaa cattccagag
     2401 acagtctcct tcatatacct ccacccctga gtggcttaca gttctgggat gaaccctcct
     2461 aggagatgcc agaggataga ggcccagagt ccttgctcta ggggacctga aggggagcgc
     2521 ctcactctgc actgttagca ggatggcagc ttcaacactc acatcagtga tccgggaaga
     2581 gaagcaagcc acccacagca tctctccagg aaaccaccca ggtccctctg tccctcatcc
     2641 ctgtcaggtt tcccagatgc catgccgccc tctccacacc agcttggaac catgggggag
     2701 tttttaatta aatatttaaa actactt
//
