LOCUS       NM_001287386            2372 bp    mRNA    linear   ROD 18-MAR-2025
DEFINITION  Mus musculus glucokinase (Gck), transcript variant 2, mRNA.
ACCESSION   NM_001287386 XM_006514445
VERSION     NM_001287386.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Waite,E.L., Tigue,M., Yu,M., Lahori,D., Kelly,K., May,C.L.,
            Naji,A., Roman,J., Doliba,N., Avrahami,D., Nguyen-Ngoc,K.V.,
            Sander,M., Glaser,B. and Kaestner,K.H.
  TITLE     The IsletTester Mouse: An Immunodeficient Model With Stable
            Hyperglycemia for the Study of Human Islets
  JOURNAL   Diabetes 74 (3), 332-342 (2025)
   PUBMED   39571094
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Yang,J., Zhu,L., Pan,H., Ueharu,H., Toda,M., Yang,Q., Hallett,S.A.,
            Olson,L.E. and Mishina,Y.
  TITLE     A BMP-controlled metabolic/epigenetic signaling cascade directs
            midfacial morphogenesis
  JOURNAL   J Clin Invest 134 (8), e165787 (2024)
   PUBMED   38466355
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2372)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2372)
  AUTHORS   Liu,B., Hua,D., Shen,L., Li,T., Tao,Z., Fu,C., Tang,Z., Yang,J.,
            Zhang,L., Nie,A., Jiang,Y., Wang,J., Li,Y., Gu,Y. and Ning,G.
  TITLE     NPC1 is required for postnatal islet beta cell differentiation by
            maintaining mitochondria turnover
  JOURNAL   Theranostics 14 (5), 2058-2074 (2024)
   PUBMED   38505613
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2372)
  AUTHORS   Martinez-Garza,U., Choi,J., Scafidi,S. and Wolfgang,M.J.
  TITLE     Proteomics identifies the developmental regulation of HKDC1 in
            liver of pigs and mice
  JOURNAL   Am J Physiol Regul Integr Comp Physiol 325 (4), R389-R400 (2023)
   PUBMED   37545422
REFERENCE   6  (bases 1 to 2372)
  AUTHORS   Efrat,S., Leiser,M., Wu,Y.J., Fusco-DeMane,D., Emran,O.A.,
            Surana,M., Jetton,T.L., Magnuson,M.A., Weir,G. and Fleischer,N.
  TITLE     Ribozyme-mediated attenuation of pancreatic beta-cell glucokinase
            expression in transgenic mice results in impaired glucose-induced
            insulin secretion
  JOURNAL   Proc Natl Acad Sci U S A 91 (6), 2051-2055 (1994)
   PUBMED   7510884
REFERENCE   7  (bases 1 to 2372)
  AUTHORS   Hughes,S.D., Quaade,C., Milburn,J.L., Cassidy,L. and Newgard,C.B.
  TITLE     Expression of normal and novel glucokinase mRNAs in anterior
            pituitary and islet cells
  JOURNAL   J Biol Chem 266 (7), 4521-4530 (1991)
   PUBMED   1999433
REFERENCE   8  (bases 1 to 2372)
  AUTHORS   James,P.A., Lavender,F.L., Lawrence,G.M. and Walker,D.G.
  TITLE     Comparison of glucokinase in C3H/He and C58 mice that differ in
            their hepatic activity
  JOURNAL   Biochem Genet 23 (7-8), 525-538 (1985)
   PUBMED   3931624
REFERENCE   9  (bases 1 to 2372)
  AUTHORS   Lavender,F.L., James,P.A. and Walker,D.G.
  TITLE     Hepatic glucokinase activity and circulating insulin concentrations
            in two inbred mouse strains
  JOURNAL   Diabetologia 25 (2), 114-119 (1983)
   PUBMED   6354813
REFERENCE   10 (bases 1 to 2372)
  AUTHORS   Coleman,D.L.
  TITLE     Genetic control of glucokinase activity in mice
  JOURNAL   Biochem Genet 15 (3-4), 297-305 (1977)
   PUBMED   141272
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL645469.18.
            
            On Jan 5, 2014 this sequence version replaced XM_006514445.1.
            
            Transcript Variant: This variant (2) contains an alternate first
            exon and initiates translation at an alternate start codon,
            compared to variant 1. The encoded isoform (2) is expressed in
            liver and has a distinct N-terminus compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR2680379.169094.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849386 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-169               AL645469.18        26930-27098         c
            170-332             AL645469.18        22825-22987         c
            333-487             AL645469.18        22241-22395         c
            488-607             AL645469.18        21046-21165         c
            608-703             AL645469.18        18646-18741         c
            704-803             AL645469.18        18440-18539         c
            804-987             AL645469.18        16324-16507         c
            988-1143            AL645469.18        15063-15218         c
            1144-1377           AL645469.18        14048-14281         c
            1378-2372           AL645469.18        12783-13777         c
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 3.88 cM"
     gene            1..2372
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /note="glucokinase"
                     /db_xref="GeneID:103988"
                     /db_xref="MGI:MGI:1270854"
     exon            1..169
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     CDS             125..1522
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /EC_number="2.7.1.1"
                     /note="isoform 2 is encoded by transcript variant 2; HK
                     IV; hexokinase-4; hexokinase-D; hexokinase type IV"
                     /codon_start=1
                     /product="hexokinase-4 isoform 2"
                     /protein_id="NP_001274315.1"
                     /db_xref="CCDS:CCDS70135.1"
                     /db_xref="GeneID:103988"
                     /db_xref="MGI:MGI:1270854"
                     /translation="MAVDTTRRGAQSLTLVEQILAEFQLQEEDLKKVMSRMQKEMDRG
                     LKLETHQEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEAGQWSVK
                     TKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDID
                     KGILLNWTKGFKASGAEGNNIVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDR
                     QCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGNSGELDEFLLEYDRM
                     VDESSVNPGQQLYEKIIGGKYMGELVRLVLLKLVEENLLFHGEASEQLRTRGAFETRF
                     VSQVESDSGDRRQILNILSTLGLRPSVADCDIVRRACESVSTRAAHMCSAGLAGVINR
                     MRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPNCEITFIESEEGSGRGAA
                     LVSAVACKKACMLGQ"
     exon            170..332
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     exon            333..487
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     exon            488..607
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     exon            608..703
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     exon            704..803
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     exon            804..987
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1143
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1377
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..2372
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2349..2354
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /note="hexamer: ATTAAA"
     polyA_site      2372
                     /gene="Gck"
                     /gene_synonym="Gk; GLK; Gls006; HK4; HKIV; Hlb62; HXKP;
                     MODY2"
                     /note="major polyA site"
ORIGIN      
        1 ttagtgcaga agtccttggc tgcctgtctt ttgcaacact cagccagaca gtcctcaccc
       61 acagcaggtg gcctcgggag tcaggaacat ctctacttcc ccaacgaccc ctgcttatcc
      121 tcagatggct gtggatacta caaggagggg agcccagtcg ttgactctgg tagagcagat
      181 cctggcagag ttccagctgc aggaggaaga cctgaagaag gtgatgagcc ggatgcagaa
      241 ggagatggac cgtggcctga agctggagac ccatcaggag gccagtgtaa agatgttgcc
      301 cacctacgtg cgttccaccc cagaaggctc agaagttgga gactttctct ccttagacct
      361 gggaggaacc aacttcaggg tgatgctggt gaaagtgggg gagggggagg caggacagtg
      421 gagcgtgaag acgaaacacc agatgtattc catccccgag gacgccatga cgggcactgc
      481 ggagatgctc tttgactaca tctctgagtg catctctgac ttcctggaca agcatcagat
      541 gaaacacaag aaactacccc tgggcttcac cttctccttc cctgtaaggc acgaagacat
      601 agacaagggc atcctgctca actggaccaa gggcttcaag gcctccggag cagaagggaa
      661 caacatcgtg ggacttctcc gagatgctat caagaggaga ggggactttg agatggatgt
      721 ggtggcaatg gtgaatgaca cggtggccac aatgatctcc tgctactatg aagaccgcca
      781 atgtgaggtc ggcatgattg tgggcaccgg ctgcaacgcc tgctacatgg aggagatgca
      841 gaatgtggag ctggtggaag gcgatgaggg gcgcatgtgt gtcaacacag agtggggcgc
      901 cttcgggaac tccggtgagc tggacgagtt cctcctggag tacgaccgga tggtggatga
      961 gagctcagtg aaccccggtc agcagctgta cgaaaagatc attggcggaa agtacatggg
     1021 cgagctggta cgacttgtgc tgctcaagct ggtagaggag aatcttctgt tccacggaga
     1081 ggcctcagag cagctgcgca cacgtggtgc ttttgagacc cgttttgtgt cgcaggtgga
     1141 gagcgactct ggggaccgaa ggcagatcct taacatcctg agcactctgg gccttcgacc
     1201 ctctgtcgcc gactgcgaca ttgtgcgccg tgcctgtgaa agcgtgtcca ctcgcgccgc
     1261 ccacatgtgc tcagcaggac tagcgggggt cataaatcgc atgcgcgaaa gccgcagtga
     1321 ggacgtgatg cgcatcacgg tgggcgtgga tggctccgtg tacaagctgc acccgagctt
     1381 caaggagcgg tttcacgcca gtgtgcgcag gctgacaccc aactgcgaaa tcaccttcat
     1441 tgaatcagag gagggcagcg gcaggggagc cgcactggtc tctgcggtgg cctgcaagaa
     1501 ggcttgcatg ctgggccagt gaaatccagg caaggacagg gacctgggtt ccacggggac
     1561 tccacacccc acaaatgctc ccagtccact agggcaggag acctattctg ctgctacccc
     1621 tggaaaatgg ggagaggccc ctgcaagcca agtcagccag tgagacagcc ctagggctct
     1681 cagcctgggg caaggggcaa gaggatcagc ggcaccaaaa gcctttcttg ctagaatcaa
     1741 ctacagagaa aggtgaagca cactcaggtc ttgctctttc agcttctggc ctcccacagc
     1801 tgtgggtctg gcctcccaag ggagtgcctc ctggacttgc aatggcctgg cttccctggg
     1861 aacacatctc catggggagg tagcttcagc agcttggcca gaccagacct gggcccccag
     1921 aagagtaagg gctgcccaga cctggctgtt ttcttgcctg tggctgaaga ggctgcaaaa
     1981 ccatgggaga ccgactatct agctacatgg aggggacttt ccaggccaca aacattccag
     2041 agacagtctc cttcatatac ctccacccct gagtggctta cagttctggg atgaaccctc
     2101 ctaggagatg ccagaggata gaggcccaga gtccttgctc taggggacct gaaggggagc
     2161 gcctcactct gcactgttag caggatggca gcttcaacac tcacatcagt gatccgggaa
     2221 gagaagcaag ccacccacag catctctcca ggaaaccacc caggtccctc tgtccctcat
     2281 ccctgtcagg tttcccagat gccatgccgc cctctccaca ccagcttgga accatggggg
     2341 agtttttaat taaatattta aaactactta aa
//
