LOCUS       NM_013847               2373 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus glycine C-acetyltransferase
            (2-amino-3-ketobutyrate-coenzyme A ligase) (Gcat), transcript
            variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_013847
VERSION     NM_013847.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Tani,H., Ohnishi,S., Shitara,H., Mito,T., Yamaguchi,M.,
            Yonekawa,H., Hashizume,O., Ishikawa,K., Nakada,K. and Hayashi,J.I.
  TITLE     Mice deficient in the Shmt2 gene have mitochondrial respiration
            defects and are embryonic lethal
  JOURNAL   Sci Rep 8 (1), 425 (2018)
   PUBMED   29323231
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Hoshino,A. and Fujii,H.
  TITLE     Nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase
            by cold and osmotic stress
  JOURNAL   Cell Stress Chaperones 12 (2), 186-191 (2007)
   PUBMED   17688197
  REMARK    GeneRIF: Nuclear translocation of 2-amino-3-ketobutyrate coenzyme A
            ligase (KBL) was detected in response to cold and osmotic stresses.
REFERENCE   3  (bases 1 to 2373)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   4  (bases 1 to 2373)
  AUTHORS   Edgar,A.J. and Polak,J.M.
  TITLE     Molecular cloning of the human and murine 2-amino-3-ketobutyrate
            coenzyme A ligase cDNAs
  JOURNAL   Eur J Biochem 267 (6), 1805-1812 (2000)
   PUBMED   10712613
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL589670.21, AK087433.1, AK075782.1 and AK013138.1.
            
            On Jun 18, 2009 this sequence version replaced NM_013847.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC024107.1, AK013138.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                AL589670.21        107004-107044       c
            42-1648             AK087433.1         1-1607
            1649-1737           AK075782.1         1589-1677
            1738-2371           AK013138.1         1693-2326
            2372-2373           AL589670.21        99562-99563         c
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 37.7 cM"
     gene            1..2373
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /note="glycine C-acetyltransferase
                     (2-amino-3-ketobutyrate-coenzyme A ligase)"
                     /db_xref="GeneID:26912"
                     /db_xref="MGI:MGI:1349389"
     exon            1..247
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..1311
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /EC_number="2.3.1.29"
                     /note="isoform a is encoded by transcript variant 1;
                     2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial;
                     AKB ligase; glycine acetyltransferase; aminoacetone
                     synthase"
                     /codon_start=1
                     /product="2-amino-3-ketobutyrate coenzyme A ligase,
                     mitochondrial isoform a"
                     /protein_id="NP_038875.3"
                     /db_xref="CCDS:CCDS27630.1"
                     /db_xref="GeneID:26912"
                     /db_xref="MGI:MGI:1349389"
                     /translation="MWASFMWHGALSPGRRAHSALAQLRCILDSELEGIRGAGTWKSE
                     RVITSRQGPSIRVDGISGGILNFCANNYLGLSSHPAVIQAGLQTLEEFGAGLSSTRFI
                     CGTQSIHKNLEAKIAHFHQREDAILYPSCFDANAGLFEALLTPEDAVLSDELNHASII
                     DGIRLCKAHKYRYRHLDMADLEAKLKEAQKHRLRLVATDGAFSMDGDIAPLQDICRLA
                     AQYGALVFVDECHATGFLGPTGRGTDELLGVMDQVTIINSTLGKALGGASGGYTTGPE
                     PLVSLLRQRSRPYLFSNSLPPAVVGCASKALDLLMESNAIIQSMAAKTRRFRSKMEAA
                     GFTVSGADHPICPVMLGDARLSSQMADDMLKKGIFVIGFSYPVVPKGKARIRVQISAV
                     HSEEDIDRCVEAFVEVGRLHGALP"
     exon            248..378
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /inference="alignment:Splign:2.1.0"
     exon            379..480
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /inference="alignment:Splign:2.1.0"
     exon            481..627
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /inference="alignment:Splign:2.1.0"
     exon            628..782
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /inference="alignment:Splign:2.1.0"
     exon            783..865
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /inference="alignment:Splign:2.1.0"
     exon            866..1037
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1159
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..2373
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1479
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /note="major polyA site"
     regulatory      1614..1619
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /note="hexamer: CATAAA"
     polyA_site      1655
                     /gene="Gcat"
                     /gene_synonym="Kbl"
     regulatory      1721..1726
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /note="hexamer: GATAAA"
     polyA_site      1743
                     /gene="Gcat"
                     /gene_synonym="Kbl"
     regulatory      2341..2346
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /note="hexamer: ATTAAA"
     polyA_site      2370
                     /gene="Gcat"
                     /gene_synonym="Kbl"
ORIGIN      
        1 gtcacgtgac ggggagcact agacttcagg ctggccagcg cactttagcc tgtgggcgcg
       61 atgtgggcca gcttcatgtg gcacggggcg ctgtcccctg gccgccgcgc acattcagcg
      121 ctggctcagt tgcgctgcat cctggacagc gaactggaag ggatccgcgg agccggcacc
      181 tggaagagtg agcgtgtgat cacgtcccgc cagggaccga gcatccgcgt ggacggcatc
      241 tcgggaggaa tcctcaactt ctgtgccaat aactacctgg gcctgagcag ccaccctgca
      301 gtgatccagg caggtctgca gactctggag gagtttggag ctggactcag ttctactcga
      361 tttatctgtg ggacccagag catccataag aatctagaag ccaagatagc ccacttccac
      421 cagcgtgagg acgccatcct ctatcccagc tgtttcgatg ccaacgctgg cctatttgag
      481 gctttgctga ccccagagga tgcggttctg tcagatgagc tgaaccatgc ttccatcatt
      541 gacggcatcc gactgtgcaa ggcccacaag taccgttacc gccacctgga catggctgac
      601 ctggaagcca agctgaagga ggctcagaag cacaggctgc gcctggtggc cacagatgga
      661 gctttctcca tggatggtga catcgctccc ctacaggaca tctgccgcct tgccgcccaa
      721 tatggtgccc tggtctttgt ggatgaatgc catgccactg gctttcttgg gcccacagga
      781 cggggcacag atgagctgct aggtgtcatg gaccaggtca ccatcatcaa ctccaccctg
      841 gggaaggcgc tgggtggagc atcaggggga tataccaccg ggcctgagcc actggtgtcc
      901 ctgctacggc agcgttctcg gccctacctc ttctccaaca gcctgccccc tgctgtagtc
      961 ggctgtgctt ctaaggccct ggacctgctc atggagagca atgccatcat ccagtctatg
     1021 gctgccaaga cccggcggtt ccgcagtaag atggaagctg ctggttttac tgtctcagga
     1081 gccgatcacc ccatctgccc tgtgatgctg ggagatgcac ggttgtcttc tcaaatggca
     1141 gatgacatgt taaagaaagg tatctttgtc atcggattta gctaccctgt ggtccctaag
     1201 ggcaaggccc ggatccgggt gcagatctca gctgtacaca gtgaggaaga cattgaccgc
     1261 tgtgtggaag ccttcgtgga ggtggggcgg ctgcatggag ctctgcccta agctcttggt
     1321 agggacctgc agagccaaag ttcctccact gccgctgaca tagggctcct ctggtcagcc
     1381 cagatcagag gctgtgactc aacccagact tctagagcct ggttgaaaat gggcctgtac
     1441 ttgtcatcag agagacaaca atgtggaatt tggctatgac acttcagtct gctttattct
     1501 gggtaggcgt acctctggct acctgaagaa ccaggcaggg gctggatctc gcatgaggtg
     1561 catcttcccc caaactaagt cgggatgctg tgtctgggag ccaccgtttc gtacataaag
     1621 cattctgtgc tgctgtgcag ggcagtggag aaccagccct gtggaccctt tctgcagcct
     1681 aagctgggtg ggcaccttgt ctcctgtgcc ctagcagaag gataaaatgt aactgccaaa
     1741 tgagaattgt tcctagacct gtgttcccta ggagagggca gaggttcagg ccccagaaag
     1801 aagaaagcag atcacccctg aaggacatcg aatccttgtt ttccagagct ctttgtcctt
     1861 aaccccgtgg ccttaggccc ctgtgcaggt cccagtttcc ccccacttta gagagctgat
     1921 gttccatagg gacacctttc tgtctctgat tcaaaagccg atagtctgtc tgatggttca
     1981 gccgccaggg ggcgcagcgc gttcacgctg ccgccgctgg ctacttgggg gccttggatc
     2041 aggctgttgg gcagccctca agccaggcca gaagggctgg tttgctgcta cgaaggccct
     2101 gtccttctac cctgtcttcc taccggcaac catcacacct gctgctccat taccataggt
     2161 gtggcctcct tcccattttg ctgaccttgc tttactgtgg gaaaggaggg gtggaatctc
     2221 gcaggctcag ccccgcaggc ttatgactgc tgctgtaggc tcagtgtggg ggtgtggcct
     2281 tgggtagctc cctagctatc tagaagtaga acgtggcagg caagtaggac ttacggtttc
     2341 attaaaaaaa caacaatgca aaaccaaaaa cag
//
