LOCUS       NP_038875                416 aa            linear   ROD 03-JUN-2024
DEFINITION  2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial isoform a
            [Mus musculus].
ACCESSION   NP_038875
VERSION     NP_038875.3
DBSOURCE    REFSEQ: accession NM_013847.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 416)
  AUTHORS   Tani,H., Ohnishi,S., Shitara,H., Mito,T., Yamaguchi,M.,
            Yonekawa,H., Hashizume,O., Ishikawa,K., Nakada,K. and Hayashi,J.I.
  TITLE     Mice deficient in the Shmt2 gene have mitochondrial respiration
            defects and are embryonic lethal
  JOURNAL   Sci Rep 8 (1), 425 (2018)
   PUBMED   29323231
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 416)
  AUTHORS   Hoshino,A. and Fujii,H.
  TITLE     Nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase
            by cold and osmotic stress
  JOURNAL   Cell Stress Chaperones 12 (2), 186-191 (2007)
   PUBMED   17688197
  REMARK    GeneRIF: Nuclear translocation of 2-amino-3-ketobutyrate coenzyme A
            ligase (KBL) was detected in response to cold and osmotic stresses.
REFERENCE   3  (residues 1 to 416)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   4  (residues 1 to 416)
  AUTHORS   Edgar,A.J. and Polak,J.M.
  TITLE     Molecular cloning of the human and murine 2-amino-3-ketobutyrate
            coenzyme A ligase cDNAs
  JOURNAL   Eur J Biochem 267 (6), 1805-1812 (2000)
   PUBMED   10712613
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL589670.21, AK087433.1, AK075782.1 and AK013138.1.
            
            On Jun 18, 2009 this sequence version replaced NP_038875.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC024107.1, AK013138.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 37.7 cM"
     Protein         1..416
                     /product="2-amino-3-ketobutyrate coenzyme A ligase,
                     mitochondrial isoform a"
                     /EC_number="2.3.1.29"
                     /note="2-amino-3-ketobutyrate coenzyme A ligase,
                     mitochondrial; AKB ligase; glycine acetyltransferase;
                     aminoacetone synthase"
                     /calculated_mol_wt=42850
     transit_peptide 1..18
                     /note="Mitochondrion.
                     /evidence=ECO:0000250|UniProtKB:Q0P5L8; propagated from
                     UniProtKB/Swiss-Prot (O88986.2)"
                     /calculated_mol_wt=2098
     mat_peptide     19..416
                     /product="2-amino-3-ketobutyrate coenzyme A ligase,
                     mitochondrial. /id=PRO_0000001247"
                     /note="propagated from UniProtKB/Swiss-Prot (O88986.2)"
                     /calculated_mol_wt=42850
     Region          24..415
                     /region_name="AAT_I"
                     /note="Aspartate aminotransferase (AAT) superfamily (fold
                     type I) of pyridoxal phosphate (PLP)-dependent enzymes.
                     PLP combines with an alpha-amino acid to form a compound
                     called a Schiff base or aldimine intermediate, which
                     depending on the reaction, is the...; cl18945"
                     /db_xref="CDD:450240"
     Site            42
                     /site_type="acetylation"
                     /note="N6-acetyllysine, alternate.
                     /evidence=ECO:0007744|PubMed:23576753; propagated from
                     UniProtKB/Swiss-Prot (O88986.2)"
     Site            order(70,130..132,156,199,203..204,228,230..231,259,262,
                     386)
                     /site_type="active"
                     /note="substrate-cofactor binding pocket [active]"
                     /db_xref="CDD:99747"
     Site            order(130..132,156,199,203,228,230..231,259,262)
                     /site_type="other"
                     /note="pyridoxal 5'-phosphate binding site [chemical
                     binding]"
                     /db_xref="CDD:99747"
     Site            184
                     /site_type="acetylation"
                     /note="N6-acetyllysine, alternate.
                     /evidence=ECO:0007744|PubMed:23576753; propagated from
                     UniProtKB/Swiss-Prot (O88986.2)"
     Site            262
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:99747"
     Site            380
                     /site_type="acetylation"
                     /note="N6-acetyllysine, alternate.
                     /evidence=ECO:0007744|PubMed:23576753; propagated from
                     UniProtKB/Swiss-Prot (O88986.2)"
     CDS             1..416
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /coded_by="NM_013847.4:61..1311"
                     /note="isoform a is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS27630.1"
                     /db_xref="GeneID:26912"
                     /db_xref="MGI:MGI:1349389"
ORIGIN      
        1 mwasfmwhga lspgrrahsa laqlrcilds elegirgagt wkservitsr qgpsirvdgi
       61 sggilnfcan nylglsshpa viqaglqtle efgaglsstr ficgtqsihk nleakiahfh
      121 qredailyps cfdanaglfe alltpedavl sdelnhasii dgirlckahk yryrhldmad
      181 leaklkeaqk hrlrlvatdg afsmdgdiap lqdicrlaaq ygalvfvdec hatgflgptg
      241 rgtdellgvm dqvtiinstl gkalggasgg yttgpeplvs llrqrsrpyl fsnslppavv
      301 gcaskaldll mesnaiiqsm aaktrrfrsk meaagftvsg adhpicpvml gdarlssqma
      361 ddmlkkgifv igfsypvvpk gkarirvqis avhseedidr cveafvevgr lhgalp
//
