LOCUS       NM_001161712            1763 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Mus musculus glycine C-acetyltransferase
            (2-amino-3-ketobutyrate-coenzyme A ligase) (Gcat), transcript
            variant 2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001161712
VERSION     NM_001161712.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Tani,H., Ohnishi,S., Shitara,H., Mito,T., Yamaguchi,M.,
            Yonekawa,H., Hashizume,O., Ishikawa,K., Nakada,K. and Hayashi,J.I.
  TITLE     Mice deficient in the Shmt2 gene have mitochondrial respiration
            defects and are embryonic lethal
  JOURNAL   Sci Rep 8 (1), 425 (2018)
   PUBMED   29323231
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Hoshino,A. and Fujii,H.
  TITLE     Nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase
            by cold and osmotic stress
  JOURNAL   Cell Stress Chaperones 12 (2), 186-191 (2007)
   PUBMED   17688197
  REMARK    GeneRIF: Nuclear translocation of 2-amino-3-ketobutyrate coenzyme A
            ligase (KBL) was detected in response to cold and osmotic stresses.
REFERENCE   3  (bases 1 to 1763)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   4  (bases 1 to 1763)
  AUTHORS   Edgar,A.J. and Polak,J.M.
  TITLE     Molecular cloning of the human and murine 2-amino-3-ketobutyrate
            coenzyme A ligase cDNAs
  JOURNAL   Eur J Biochem 267 (6), 1805-1812 (2000)
   PUBMED   10712613
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL589670.21, AK076469.1 and AK013138.1.
            
            Transcript Variant: This variant (2) uses an alternate 3' exon and
            differs in the 3' coding region and 3' UTR, compared to variant 1.
            The resulting protein (isoform b) has a shorter and distinct
            C-terminus, compared to isoform a.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK076469.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                AL589670.21        107018-107044       c
            28-214              AK076469.1         2-188
            215-870             AK013138.1         170-825
            871-1763            AK076469.1         844-1736
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 37.7 cM"
     gene            1..1763
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /note="glycine C-acetyltransferase
                     (2-amino-3-ketobutyrate-coenzyme A ligase)"
                     /db_xref="GeneID:26912"
                     /db_xref="MGI:MGI:1349389"
     exon            1..247
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..1209
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /EC_number="2.3.1.29"
                     /note="isoform b is encoded by transcript variant 2;
                     2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial;
                     AKB ligase; glycine acetyltransferase; aminoacetone
                     synthase"
                     /codon_start=1
                     /product="2-amino-3-ketobutyrate coenzyme A ligase,
                     mitochondrial isoform b"
                     /protein_id="NP_001155184.1"
                     /db_xref="CCDS:CCDS49666.1"
                     /db_xref="GeneID:26912"
                     /db_xref="MGI:MGI:1349389"
                     /translation="MWASFMWHGALSPGRRAHSALAQLRCILDSELEGIRGAGTWKSE
                     RVITSRQGPSIRVDGISGGILNFCANNYLGLSSHPAVIQAGLQTLEEFGAGLSSTRFI
                     CGTQSIHKNLEAKIAHFHQREDAILYPSCFDANAGLFEALLTPEDAVLSDELNHASII
                     DGIRLCKAHKYRYRHLDMADLEAKLKEAQKHRLRLVATDGAFSMDGDIAPLQDICRLA
                     AQYGALVFVDECHATGFLGPTGRGTDELLGVMDQVTIINSTLGKALGGASGGYTTGPE
                     PLVSLLRQRSRPYLFSNSLPPAVVGCASKALDLLMESNAIIQSMAAKTRRFRSKMEAA
                     GFTVSGADHPICPVMLGDARLSSQMADDMLKKGKWLTSRTFRWTAQGA"
     exon            248..378
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /inference="alignment:Splign:2.1.0"
     exon            379..480
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /inference="alignment:Splign:2.1.0"
     exon            481..627
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /inference="alignment:Splign:2.1.0"
     exon            628..782
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /inference="alignment:Splign:2.1.0"
     exon            783..865
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /inference="alignment:Splign:2.1.0"
     exon            866..1037
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1159
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1763
                     /gene="Gcat"
                     /gene_synonym="Kbl"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtcacgtgac ggggagcact agacttcagg ctggccagcg cactttagcc tgtgggcgcg
       61 atgtgggcca gcttcatgtg gcacggggcg ctgtcccctg gccgccgcgc acattcagcg
      121 ctggctcagt tgcgctgcat cctggacagc gaactggaag ggatccgcgg agccggcacc
      181 tggaagagtg agcgtgtgat cacgtcccgc cagggaccga gcatccgcgt ggacggcatc
      241 tcgggaggaa tcctcaactt ctgtgccaat aactacctgg gcctgagcag ccaccctgca
      301 gtgatccagg caggtctgca gactctggag gagtttggag ctggactcag ttctactcga
      361 tttatctgtg ggacccagag catccataag aatctagaag ccaagatagc ccacttccac
      421 cagcgtgagg acgccatcct ctatcccagc tgtttcgatg ccaacgctgg cctatttgag
      481 gctttgctga ccccagagga tgcggttctg tcagatgagc tgaaccatgc ttccatcatt
      541 gacggcatcc gactgtgcaa ggcccacaag taccgttacc gccacctgga catggctgac
      601 ctggaagcca agctgaagga ggctcagaag cacaggctgc gcctggtggc cacagatgga
      661 gctttctcca tggatggtga catcgctccc ctacaggaca tctgccgcct tgccgcccaa
      721 tatggtgccc tggtctttgt ggatgaatgc catgccactg gctttcttgg gcccacagga
      781 cggggcacag atgagctgct aggtgtcatg gaccaggtca ccatcatcaa ctccaccctg
      841 gggaaggcgc tgggtggagc atcaggggga tataccaccg ggcctgagcc actggtgtcc
      901 ctgctacggc agcgttctcg gccctacctc ttctccaaca gcctgccccc tgctgtagtc
      961 ggctgtgctt ctaaggccct ggacctgctc atggagagca atgccatcat ccagtctatg
     1021 gctgccaaga cccggcggtt ccgcagtaag atggaagctg ctggttttac tgtctcagga
     1081 gccgatcacc ccatctgccc tgtgatgctg ggagatgcac ggttgtcttc tcaaatggca
     1141 gatgacatgt taaagaaagg aaaatggctg acatccagaa catttcgctg gacagcccag
     1201 ggagcgtagg ggctgtggca gtgccggtgg tctttgccct catcttcctg ttgggcatgg
     1261 tgggtaatgg gctggtgttg gccgtgcttc tgcagcctgg cccaagtgcc tggcaggaac
     1321 cgggcagtac cacggatctc ttcatcctca acttggcggt ggccgacctt tgcttcatcc
     1381 tgtgctgcgt gcccttccag gcggccatct acacactgga tgcctggctc tttggggctt
     1441 tcgtgtgcaa gacggtacat ctgctcatct acctcaccat gtatgccagc agcttcaccc
     1501 tggcggccgt ctcagtggat aggtgagctg tacctgggtc aggccagaga gggacctgga
     1561 cagattctta ttagctagcg gaatataact agggaccaga ctttggtggg aggaaaggaa
     1621 ggccttagtg gcacctgccc ttccggcctc cagtgtggac ttccagaggg catttctaag
     1681 ccccaaagtg tcagcgcagg gggccaggta taaattggaa ttgtgtagta acaaaagaaa
     1741 ctcccaatac actcgtcaga tgc
//
