LOCUS       NM_001359079            4637 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus guanylate binding protein 4 (Gbp4), transcript variant
            3, mRNA.
ACCESSION   NM_001359079 XM_006534803
VERSION     NM_001359079.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Simpson,D.S., Pang,J., Weir,A., Kong,I.Y., Fritsch,M., Rashidi,M.,
            Cooney,J.P., Davidson,K.C., Speir,M., Djajawi,T.M., Hughes,S.,
            Mackiewicz,L., Dayton,M., Anderton,H., Doerflinger,M., Deng,Y.,
            Huang,A.S., Conos,S.A., Tye,H., Chow,S.H., Rahman,A., Norton,R.S.,
            Naderer,T., Nicholson,S.E., Burgio,G., Man,S.M., Groom,J.R.,
            Herold,M.J., Hawkins,E.D., Lawlor,K.E., Strasser,A., Silke,J.,
            Pellegrini,M., Kashkar,H., Feltham,R. and Vince,J.E.
  TITLE     Interferon-gamma primes macrophages for pathogen ligand-induced
            killing via a caspase-8 and mitochondrial cell death pathway
  JOURNAL   Immunity 55 (3), 423-441 (2022)
   PUBMED   35139355
REFERENCE   3  (bases 1 to 4637)
  AUTHORS   Hu,Y., Wang,J., Yang,B., Zheng,N., Qin,M., Ji,Y., Lin,G., Tian,L.,
            Wu,X., Wu,L. and Sun,B.
  TITLE     Guanylate binding protein 4 negatively regulates virus-induced type
            I IFN and antiviral response by targeting IFN regulatory factor 7
  JOURNAL   J Immunol 187 (12), 6456-6462 (2011)
   PUBMED   22095711
  REMARK    GeneRIF: GBP4 is a negative regulator of virus-triggered type I
            interferon (IFN-I) production, interacting as a novel protein
            targeting interferon regulatory factor (IRF)-7 and inhibiting its
            function.
REFERENCE   4  (bases 1 to 4637)
  AUTHORS   Kim,B.H., Shenoy,A.R., Kumar,P., Das,R., Tiwari,S. and
            MacMicking,J.D.
  TITLE     A family of IFN-gamma-inducible 65-kD GTPases protects against
            bacterial infection
  JOURNAL   Science 332 (6030), 717-721 (2011)
   PUBMED   21551061
REFERENCE   5  (bases 1 to 4637)
  AUTHORS   Degrandi,D., Konermann,C., Beuter-Gunia,C., Kresse,A., Wurthner,J.,
            Kurig,S., Beer,S. and Pfeffer,K.
  TITLE     Extensive characterization of IFN-induced GTPases mGBP1 to mGBP10
            involved in host defense
  JOURNAL   J Immunol 179 (11), 7729-7740 (2007)
   PUBMED   18025219
REFERENCE   6  (bases 1 to 4637)
  AUTHORS   Konermann,C., Kresse,A., Beuter-Gunia,C., Wurthner,J., Degrandi,D.,
            Pfeffer,K. and Beer,S.
  TITLE     In silico and in vitro characterization of mGBP4 splice variants
  JOURNAL   DNA Cell Biol 26 (12), 847-851 (2007)
   PUBMED   17919070
  REMARK    GeneRIF: Two splice variants of mGBP4 could be detected. One
            variant led to a premature stop codon after 312 bp. The second
            variant resulted in a transcript with a disrupted G2 domain.
REFERENCE   7  (bases 1 to 4637)
  AUTHORS   Olszewski,M.A., Gray,J. and Vestal,D.J.
  TITLE     In silico genomic analysis of the human and murine
            guanylate-binding protein (GBP) gene clusters
  JOURNAL   J Interferon Cytokine Res 26 (5), 328-352 (2006)
   PUBMED   16689661
REFERENCE   8  (bases 1 to 4637)
  AUTHORS   Nguyen,T.T., Hu,Y., Widney,D.P., Mar,R.A. and Smith,J.B.
  TITLE     Murine GBP-5, a new member of the murine guanylate-binding protein
            family, is coordinately regulated with other GBPs in vivo and in
            vitro
  JOURNAL   J Interferon Cytokine Res 22 (8), 899-909 (2002)
   PUBMED   12396730
REFERENCE   9  (bases 1 to 4637)
  AUTHORS   Wynn,T.A., Nicolet,C.M. and Paulnock,D.M.
  TITLE     Identification and characterization of a new gene family induced
            during macrophage activation
  JOURNAL   J Immunol 147 (12), 4384-4392 (1991)
   PUBMED   1753106
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC123697.19 and AC144914.18.
            
            On Dec 20, 2017 this sequence version replaced XM_006534803.3.
            
            Transcript Variant: This variant (3) represents the longest
            transcript and encodes the longest isoform (3).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-107               AC123697.19        14218-14324         c
            108-206             AC123697.19        13457-13555         c
            207-442             AC144914.18        1106-1341
            443-566             AC144914.18        2961-3084
            567-676             AC144914.18        12525-12634
            677-873             AC144914.18        12820-13016
            874-1119            AC144914.18        15031-15276
            1120-1400           AC144914.18        16080-16360
            1401-1607           AC144914.18        17036-17242
            1608-1710           AC144914.18        18150-18252
            1711-1904           AC144914.18        18580-18773
            1905-4637           AC144914.18        19652-22384
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 50.68 cM"
     gene            1..4637
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
                     /note="guanylate binding protein 4"
                     /db_xref="GeneID:17472"
                     /db_xref="MGI:MGI:97072"
     exon            1..107
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
                     /inference="alignment:Splign:2.1.0"
     exon            108..206
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
                     /inference="alignment:Splign:2.1.0"
     CDS             192..2126
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
                     /note="isoform 3 is encoded by transcript variant 3;
                     guanylate-binding protein 4 homolog; guanylate nucleotide
                     binding protein 4; macrophage activation 2; GTP-binding
                     protein 4; guanine nucleotide-binding protein 4"
                     /codon_start=1
                     /product="guanylate-binding protein 4 isoform 3"
                     /protein_id="NP_001346008.1"
                     /db_xref="GeneID:17472"
                     /db_xref="MGI:MGI:97072"
                     /translation="MKVYKIHLCWQVAMTQPQMAPICLVENHNEQLSVNQEAIEILDK
                     ISQPVVVVAIVGWSHTGKSYLMNCLAGQNHVSGTLPTSQRFPSGLHRAVSDQGHLDVV
                     HAPPHQARALVLLDTEGLGDVEKGDPKNDLWIFALSVLLSSTFVYNSMNTINHQALEQ
                     LHYVTELTELIRAKSSPNPHGIKNSTEFVSFFPDFVWTVRDFMLELKLNGEDITSDEY
                     LENALKLIPGNNPRIQASNSARECIRRFFPNRKCFVFEWPTHDIELIKQLETISEDQL
                     DPTFKESAMAFASYIFTYAKIKTLREGIKVTGNGLGTLVTTYVDAINSGAVPCLDDAV
                     TTLAQRENSVAVQKAASHYSEQMAQRLSLPTDTIQELLDVHAACEKEAMAVFMEHSFK
                     DENQQFLKKLVELLREKNGLFLLKNEEASDKYCQEELDRLSKDLMDNISTFSVPGGHR
                     LYMDMREKIEHDYWQVPRKGVKASEVFQNFLQSQAIIESSILQADTALTAGQKAIAEK
                     HTKKEAAEKEQDLLRQKQKEHQEYMEAQEKRNKENLEQLRRKLEQEREQLIKDHNMML
                     EKLTKEQKTFREEGYKTQAEELRREIHQLGHNIKEMKQNGDSLVESILRSWFSFISPP
                     SESEKAISSVLSLLRKKDRL"
     exon            207..442
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
                     /inference="alignment:Splign:2.1.0"
     exon            443..566
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
                     /inference="alignment:Splign:2.1.0"
     exon            567..676
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
                     /inference="alignment:Splign:2.1.0"
     exon            677..873
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
                     /inference="alignment:Splign:2.1.0"
     exon            874..1119
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1400
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1607
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
                     /inference="alignment:Splign:2.1.0"
     exon            1608..1710
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
                     /inference="alignment:Splign:2.1.0"
     exon            1711..1904
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..4637
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2854..2859
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
                     /note="hexamer: AATATA"
     polyA_site      2884
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
                     /note="major polyA site"
     regulatory      4573..4578
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
                     /note="hexamer: AATAAA"
     regulatory      4588..4593
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
                     /note="hexamer: AATAAA"
     polyA_site      4589
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
     polyA_site      4611
                     /gene="Gbp4"
                     /gene_synonym="GBP-4; Mag-2; Mpa-2; Mpa2"
ORIGIN      
        1 cgcacataaa agaggagcaa agagagcagg aagtgaggag agcagaagtg agcctgagga
       61 ggcagcagca gctgagaact gcacttggac ctgtgctgtg ggaccaggct catctgggac
      121 tggagtgcag aggaggggag gcaagttatg tgttgtctga cctgcagagg tctgtggctg
      181 gctttaagtc tatgaaggtc tacaagattc atctatgttg gcaggttgct atgacccaac
      241 cacaaatggc tcccatttgt cttgtggaaa accacaatga acagctgtca gtgaaccagg
      301 aagccataga gattctggac aagatttctc agccagtggt agtagtggct attgttggat
      361 ggtcccatac agggaagtcc tatttgatga actgtttggc gggacagaat cacgtgagtg
      421 gcacgctgcc taccagccaa aggtttccct ctgggctcca ccgtgcagtc tcagaccaag
      481 ggcatctgga tgtggtgcat gccccacccc accaagccag agcactggtc ctcctggaca
      541 ccgagggcct gggcgatgtg gaaaagggtg atcctaagaa cgacttgtgg atctttgccc
      601 tcagtgtgct tctgagcagc accttcgtct acaacagcat gaacaccatc aaccaccaag
      661 ccctggagca gctgcattat gtcacagaac tcactgagct gatcagagca aaatcttccc
      721 caaatcccca tggaataaag aattccacag agtttgtgag tttctttcca gactttgtct
      781 ggactgttcg ggatttcatg ctggagctga agttaaatgg ggaagacatc acaagtgatg
      841 agtacctgga gaatgccctg aagctgatcc caggtaacaa tcccagaatc caagcatcca
      901 attcggccag ggaatgcatc agacgtttct ttcctaatcg gaagtgtttt gtctttgaat
      961 ggccaactca tgacatagaa ctcataaaac aacttgagac tatttcagaa gaccaattgg
     1021 atcctacgtt taaggaaagt gcaatggctt ttgcttctta tatcttcact tatgccaaga
     1081 tcaagaccct cagagaggga attaaggtca ctgggaatgg actgggtact ctggtgacaa
     1141 cctacgtgga tgccatcaac agtggagcag tgccttgtct ggatgatgct gtgacaactc
     1201 tggcccagcg tgagaactca gtagctgtgc agaaggcagc cagccactac agtgagcaga
     1261 tggcccagcg actgagcctt cccacagaca cgatccagga gctgctggat gtacatgcag
     1321 cctgcgagaa ggaagccatg gctgtcttta tggagcattc cttcaaagac gaaaatcagc
     1381 aattcctgaa gaagctggtg gaattactac gtgagaagaa tgggcttttc ctgttgaaga
     1441 atgaagaggc atctgataaa tactgccagg aagaactgga tcgactttca aaggatttga
     1501 tggacaatat ctcaacattt tctgttcctg ggggacacag gctctacatg gacatgaggg
     1561 aaaagattga acatgactac tggcaggttc ccaggaaagg ggtgaaggca agtgaagtct
     1621 tccagaactt tctgcagtca caggccatca tcgagagttc catcttgcag gcagatacag
     1681 ccctcactgc tgggcagaag gccattgcag agaagcacac caagaaggag gcagctgaga
     1741 aggagcagga tctgctaaga cagaagcaga aggagcatca ggagtatatg gaggctcaag
     1801 agaaaaggaa caaggaaaac ctagagcaac tgagaaggaa gctggagcag gagagagagc
     1861 agctcatcaa agaccataac atgatgctgg agaagctaac gaaggaacaa aagactttcc
     1921 gtgaggaagg atataagaca caagctgagg aattgcgtcg agagatccat caactgggac
     1981 ataacatcaa ggaaatgaaa caaaatggtg attcccttgt ggaaagtatt ttaagaagtt
     2041 ggttttcatt tatttcaccc ccttcagagt cagaaaaggc tataagcagc gttttatcac
     2101 tccttaggaa aaaggataga ctgtgataca cactttgtgt tattttgaag ctgcctgttt
     2161 tccattttca ttctgcacag aagattaaac ataaaaagtg ctgattaaag ggcatctggg
     2221 ccccacagaa atgagataga acagctctta cgtgtctcca cagacaatgc ttgcctttag
     2281 agaacacatt ttgaaacaat tcctatggag ggtatgcatt tagtcattac atatgatcac
     2341 tgctggccaa tttaactgca tatagattca gcaagaaaac aaggttttct gtactcctat
     2401 gaggaccatg taacccaggt agcttctggg gggattgtct caggttaata tggtagggaa
     2461 cccatcctaa gtgtgggcag caccattgct gtggaccgaa aacacggtag aaagaagaaa
     2521 tcaaatggcc ctaccacttg tggcttcctg actgtggatg ccatacaaca agccagcctg
     2581 gacttctctc agaatgcatt ttcagccttg atgaaatgta tcatcatgat tcatggacca
     2641 caacaaaccc tttattacat tggttttgtg agggcatttc ctcacaacaa gaggaaaaat
     2701 cattgatatc ataactgtga gaaacctgga aactacttaa acaaccctga gttagattgg
     2761 gaacaaattt tatatgactt tgttcttgaa gtactgtata gtcaagaaac aatgtcagtc
     2821 aagctatttg tagaatgatc taaattgagc taaaatatat ctttaaaaac taagccaaac
     2881 accatacaac tgtgtgatta ttattagaaa tactaccatg acattcaaac atactagaac
     2941 agtaatgaaa tgcctttggg aagactaata aaaagcctga tctattggct tcatcgaggg
     3001 agaataggtt ggagtaaaaa gttgaagaga tgcatacatt ccatggcgtt ctcagtctta
     3061 tacatttctt gaagagtgct gggaaagcga atttacatta cttttagtga caataagtta
     3121 aaactcacca ctgcatgatg aaacatctgc ttctagaaat gtcataagcc actagaagtc
     3181 acccaagaac aagacttgag taccaggaag cataacattt gcagcttaat gatgttttcc
     3241 tttgagttaa tgatgttttc ctttgaggct ttgctttttt aaaaataatt taaataatac
     3301 attttatgtc accaacatat caaaatatta tttccacagg cagttgatat gatttatgca
     3361 tgacattttt atatcagacc tattttatat catatttatt tatttgcaaa gtaatgagga
     3421 acctgtatca aaagagaaat gatagtattg aggataagag agtccaagag tcacatggga
     3481 ggtggacagc tcagagatgg ctctgggtca agagtgcaca attttcatgc agggtagctc
     3541 ggttcctggg gcaggtaaca actacctgta aatttaactc caagggatct gacttagttt
     3601 tctgacccct actggtcact ggtacttcca tcatatatcc ccaggttaca catatacata
     3661 catacaagta aaatttaaac aaatacactg ataaaaatga tagtgtgcct tatgagtaaa
     3721 gagacccaag gacccttatt agtgtgctca tgaatataat atatttagag tgtacccacc
     3781 cccagctcct ctaagtcatc ctcaaattca cgtcccaatt tcatgtcttt ccctcctcca
     3841 cctcttcctc tttcttcttc ctttgaataa attaatgctg gcctcatgtc cttgggtatt
     3901 agacaccatc taccagacca tggataattt agtatggccc acacccccat agaaacacac
     3961 tctccctctc ccagtcacca gatatcaact gctaatagtt cctcagctag gggtggggtc
     4021 tcatgatctc actgcctccc cttactggat gttgactagc ttaatagtgt gtatcttgtg
     4081 ccaacaaccg tatctactgt catagcccca tactttccag gaaatattat tttacaacaa
     4141 catggtctta tttttaaaat acttatgtta attatttcaa aattaaaata tagaaactct
     4201 tatggtttct ggaatcttgg agaagcctac ttctctctta atgccttgta agatgtctag
     4261 ttcaaaagaa tgaactgagt aaaggagaga ttttggtgct gtcttattgg gagccatttt
     4321 gtttccttct ttgacagcct gcctgccagg cacagtcaag gcaggtcagg gtctactcgc
     4381 agatttcttg tcctactgac tcatggaact ataagaggaa atacacttga ggctcaatgc
     4441 aaggggatgg aagttcaatt catcttgctg gttggaacag gaaatcctct ggataagaaa
     4501 ggatatcctg tgtgtatatc agctattatt taagctacct tgtgatattg tttccacggt
     4561 actgagagat ataataaagc taagaaaaat aaacttgagg ttgatgtggt attcacctgc
     4621 aaccttccca attctaa
//
