LOCUS       NM_025709               6748 bp    mRNA    linear   ROD 01-MAY-2024
DEFINITION  Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1),
            transcript variant 2, mRNA.
ACCESSION   NM_025709
VERSION     NM_025709.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6748)
  AUTHORS   Ducommun,S., Deak,M., Zeigerer,A., Goransson,O., Seitz,S.,
            Collodet,C., Madsen,A.B., Jensen,T.E., Viollet,B., Foretz,M.,
            Gut,P., Sumpton,D. and Sakamoto,K.
  TITLE     Chemical genetic screen identifies Gapex-5/GAPVD1 and STBD1 as
            novel AMPK substrates
  JOURNAL   Cell Signal 57, 45-57 (2019)
   PUBMED   30772465
  REMARK    GeneRIF: AMPK-dependent phosphorylation of Gapex-5 (also known as
            GTPase-activating protein and VPS9 domain-containing protein 1
            (GAPVD1)) on Ser902 in hepatocytes and starch-binding domain 1
            (STBD1) on Ser175 in multiple cells/tissues
REFERENCE   3  (bases 1 to 6748)
  AUTHORS   Ng,E.L., Ng,J.J., Liang,F. and Tang,B.L.
  TITLE     Rab22B is expressed in the CNS astroglia lineage and plays a role
            in epidermal growth factor receptor trafficking in A431 cells
  JOURNAL   J Cell Physiol 221 (3), 716-728 (2009)
   PUBMED   19725050
REFERENCE   4  (bases 1 to 6748)
  AUTHORS   Lodhi,I.J., Bridges,D., Chiang,S.H., Zhang,Y., Cheng,A.,
            Geletka,L.M., Weisman,L.S. and Saltiel,A.R.
  TITLE     Insulin stimulates phosphatidylinositol 3-phosphate production via
            the activation of Rab5
  JOURNAL   Mol Biol Cell 19 (7), 2718-2728 (2008)
   PUBMED   18434594
  REMARK    GeneRIF: the TC10/GAPEX-5/Rab5 axis mediates insulin-stimulated
            production of PI(3)P, which regulates trafficking of Glut4 vesicles
REFERENCE   5  (bases 1 to 6748)
  AUTHORS   Kitano,M., Nakaya,M., Nakamura,T., Nagata,S. and Matsuda,M.
  TITLE     Imaging of Rab5 activity identifies essential regulators for
            phagosome maturation
  JOURNAL   Nature 453 (7192), 241-245 (2008)
   PUBMED   18385674
REFERENCE   6  (bases 1 to 6748)
  AUTHORS   Lodhi,I.J., Chiang,S.H., Chang,L., Vollenweider,D., Watson,R.T.,
            Inoue,M., Pessin,J.E. and Saltiel,A.R.
  TITLE     Gapex-5, a Rab31 guanine nucleotide exchange factor that regulates
            Glut4 trafficking in adipocytes
  JOURNAL   Cell Metab 5 (1), 59-72 (2007)
   PUBMED   17189207
  REMARK    GeneRIF: Insulin recruits the CIP4/Gapex-5 complex to the plasma
            membrane, thus reducing Rab31 activity and permitting Glut4
            vesicles to translocate to the cell surface, where Glut4 docks and
            fuses to transport glucose into the cell.
REFERENCE   7  (bases 1 to 6748)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   8  (bases 1 to 6748)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 6748)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 6748)
  AUTHORS   Wertz,K. and Herrmann,B.G.
  TITLE     Large-scale screen for genes involved in gonad development
  JOURNAL   Mech Dev 98 (1-2), 51-70 (2000)
   PUBMED   11044607
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC060123.1 and AL845262.3.
            
            On Oct 4, 2017 this sequence version replaced NM_025709.2.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice junction compared to variant 1. The resulting isoform (2)
            has the same N- and C-termini but is shorter compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC060123.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5966              BC060123.1         1-5966
            5967-6748           AL845262.3         173383-174164       c
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 22.87 cM"
     gene            1..6748
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /note="GTPase activating protein and VPS9 domains 1"
                     /db_xref="GeneID:66691"
                     /db_xref="MGI:MGI:1913941"
     exon            1..255
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            256..549
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            550..767
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     CDS             583..4896
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /note="isoform 2 is encoded by transcript variant 2;
                     GTPase-activating protein and VPS9 domain-containing
                     protein 1; rab5-activating protein 6"
                     /codon_start=1
                     /product="GTPase-activating protein and VPS9
                     domain-containing protein 1 isoform 2"
                     /protein_id="NP_079985.2"
                     /db_xref="CCDS:CCDS15949.1"
                     /db_xref="GeneID:66691"
                     /db_xref="MGI:MGI:1913941"
                     /translation="MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRT
                     AWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSR
                     LRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFE
                     LKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETD
                     PNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCF
                     PHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPIN
                     EVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPP
                     MSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPG
                     KSSSLDMTPYSTPQMSPATTPANKKNRLPIATRSRSRSNMLMDLHMDHEGSSQETIQE
                     VQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGPSNRSNSVS
                     SLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDR
                     DISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCT
                     GSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPS
                     TPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPS
                     QSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSH
                     VLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGA
                     TSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKAPIPFRK
                     KEKQEKDKDDLGPDRFSTLTDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMAND
                     ESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALC
                     SADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRC
                     VCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVA
                     NRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQ
                     NASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRA
                     LQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGA
                     DDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDR
                     K"
     exon            768..1611
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            1612..1698
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            1699..1833
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..2023
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            2024..2184
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            2185..2251
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            2252..2377
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            2378..2551
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            2552..2692
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            2693..2827
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            2828..2947
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            2948..3025
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            3026..3390
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            3391..3478
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            3479..3559
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            3560..3682
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            3683..3812
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            3813..3922
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            3923..4082
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            4083..4270
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            4271..4484
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            4485..4557
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            4558..4755
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     exon            4756..6748
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6579..6584
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /note="hexamer: AATAAA"
     polyA_site      6602
                     /gene="Gapvd1"
                     /gene_synonym="2010005B09Rik; 4432404J10Rik; Gapex-5;
                     mKIAA1521; RAP6; RME-6"
                     /note="major polyA site"
ORIGIN      
        1 attttaaagg gctgtcaagt gggaaccaat tctccttgct tccaggcaga ataactacct
       61 gcaaagggag gaaagacaag ggaagcagat ttcagctcag cagaaggcag aactttctaa
      121 catttagagc tgtgcaacag agaggagcag gctgtactcc tgagaggtag tgagctgata
      181 ctggaacatc cgaaacagaa gtgagacgtg gaggccattc ctgcttgggt gggagctgca
      241 ccgatgacct ttaagcacca ggtacacgga gacgtgaggg cggaggatcg gcgtacaggt
      301 cctggcaagg cggttcgcga ccgggagcgg aagggcggcc gtggtccgga ccaccgatcc
      361 caggggcccc cgcggcgccg cgcacgcgca ctgctgaggg ggcagcgccg ccgcaggagg
      421 cagggaaacc gactggcgat ctgtggcggc ggctgggcgg cggtggcagt tcgcagcgag
      481 ggcgacggct ccgaggccga gaggcagtcc agcgaggacc tgggcgccgt cctccgcatg
      541 acctcaccgt gatcaacttt tgtacctttt ccgaattcaa agatggtgaa gctagatatt
      601 cacacattgg cccaccacct caagcaggaa cgcttatatg tgagctctga gaaacagctc
      661 attcagaggc taaatgccga tgtactgaag acagctgaaa agctgtatcg aacagcgtgg
      721 attgcaaagc aacagaggat taatttggat cgtcttatta taaccagtgc tgaagcttcc
      781 cctgctgagt gttgccagca tgccaagatt ttggaagata cacagtttgt tgatggatat
      841 aagcaactgg gatttcagga aacagcttat ggagaattcc tgagtcggtt aagagaaaat
      901 ccacgactta ttgcctcctc tttggttgct ggagaaaaac tcaatcagga gaacacacaa
      961 agtgttatct acactgtttt tacctccttg tatggcaact gtattatgca agaagatgaa
     1021 agctacctcc ttcaggtttt acgatacttg attgaatttg aacttaaaga aagtgacaac
     1081 cccaggcgcc ttttgaggag aggaacttgt gccttcagca tcttatttaa acttttttct
     1141 gaaggactgt tttctgccaa acttttcctt acggctactt tgcatgagcc aatcatgcag
     1201 ctcctcgtag aggatgaaga ccacctggaa acagatccaa acaagctgat agagagattc
     1261 tctccagctc agcaagaaaa actctttgga gagaaaggct cagatagatt taggcaaaag
     1321 gttcaagaaa tggtagattc caatgaggct aaactagtgg ctttggtgaa caaatttatt
     1381 ggttatctta aacagaatac atactgcttt ccacatagtt tgaggtggat tgtgtcacag
     1441 atgtacaaaa ctctttcttg tgtagacaga ttggaggttg gggaagtcag agcaatgtgt
     1501 accgatcttc tgctggcttg cttcatttgt cctgcagttg tcaatccaga gcagtatggg
     1561 ataatatctg atgctcctat taatgaggtg gcaagattta acctgatgca ggttggccgc
     1621 cttttgcagc agctagcaat gactggcact gaagaaggag atccccggac caaaaatagc
     1681 cttggaaaat ttgacaaagg ctgtgtcgct gctttccttg atgttgtaat tgggggccgt
     1741 gcagtggaga ctccgccaat gtcctctgtt aatcttctgg aaggcttgag cagaactgtg
     1801 gtgtatataa gttacagtca gcttattact ctggtaaatt ttatgaagag tgtgatgtct
     1861 ggagatcaac tgaaagaaga tagaatggct cttgacaatt tgttggcaaa cctgccccag
     1921 gctaagccag ggaagagcag cagtctggac atgactccct atagcactcc tcagatgtct
     1981 ccagcaacca ctccagccaa caagaagaat cgattaccta tagcaactcg gagcagaagc
     2041 cgaagtaata tgttaatgga cctacatatg gatcatgaag gatcatctca agaaaccatc
     2101 caggaggtac agccagaaga ggtgctggtc atttccttag ggacaggccc tcagctcact
     2161 ccagggatga tgtcagaaaa tgaggttcta aacatgcagc tttcggatgg aggacaagga
     2221 gatgtccctg ttgatgaaaa caaactccac ggtccttcca atcgctccaa ttcggtgtct
     2281 tctctcgacc tggagggaga gtctgtatct gaacttgggg caggaccttc tgggagtaac
     2341 ggagttgaag cactgcagct gttagagcac gagcaagcta caacacaaga taatcttgat
     2401 gataaactga ggaagtttga gattcgtgac atgatgggac taacagatga cagggacata
     2461 tccgagactg tgagtgagac ctggagcact gatgtcctgg gaagtgactt cgacccgaac
     2521 gttgatgaag atcgtttgca agagattgca ggtgcagcag cagagaacgt gttaggtagt
     2581 ttgctgtgcc ttccaggttc agggtcagtt ctccttgacc cttgcactgg ctctaccata
     2641 tcagagacca caagtgaagc ttggagtgta gaggtattgc caagtgactc agaggctcca
     2701 gacttaaagc aggaagagcg cctccaagag ctggagagct gttctggact gggcagcaca
     2761 tctgatgata cggatgtcag ggaggtcagt tcccgcccca gcacaccagg cctcagtgtt
     2821 gtgtccggta taagtgctac atcggaggat attcccaata agatcgaaga cctgagatct
     2881 gagtgcagct ctgattttgg gggcaaagat tctgtaacta gtccagatat ggatgacata
     2941 gcccatggcg ctcatcagct gacctctcct ccctctcagt cagagtctct gcttgccatg
     3001 tttgatccac tgtcctcaca tgaaggggcc tctgctgtgg taaggccaaa ggttcactat
     3061 gcccgaccct cgcatccacc tccggatcct ccaatcctgg aaggtgcagt gggaggaaat
     3121 gaggccaggt tgccaaactt tggctctcac gttttaactg cagctgaaat ggaggcgttc
     3181 aagcagagac actcataccc tgagagattg gttcgcagca gaagctctga tatagtgtct
     3241 tctgtgcggc ggcccatgag tgaccccagc tggaaccgac gtccagggaa tgaagaactt
     3301 cctccagctg cagccactgg tgctacttct ttggtggctg cacctcactc gtcatcttcg
     3361 tccccgagta aggactcttc tagaggagag actgaggaac gcaaggacag tgacgatgag
     3421 aggtcagaca ggagcaggcc ttggtggagg aagcgctttg tttcagctat gcctaaagct
     3481 cctataccat ttagaaagaa agaaaaacaa gaaaaagaca aagatgatct gggtcctgac
     3541 agattctcaa cactcacaga tgagcccagc cctaggctca gtgctcaagc tcaggttgct
     3601 gaagatattc tggacaagta ccggaatgcc attaaacgga ccagccccag tgaaggagca
     3661 atggcaaatg atgagagtgc agaggtcatg ggtgatggtg aaagtgccca cgactctcct
     3721 cgtgaggaag cactgcagaa catctcagcc gacgacctcc cggactccgc cagccaggca
     3781 gcccaccctc aggactctgc tttctcttac agagatgtaa aaaagaagct gaggctcgca
     3841 ctttgctctg cagactctgt ggccttccca gtgctaactc attcaacaag gaatggattg
     3901 ccagaccaca cagacccaga agacaatgaa attgtatgct tcttaaaagt tcaaatagct
     3961 gaagcaatta atttacaaga taagagctta atggctcaac ttcaagaaac aatgcgttgt
     4021 gtgtgccgct ttgacaatag gacttgtagg aagttgctgg catccattgc tgaagactac
     4081 agaaaaagag ctccgtatat tgcttatctt acccgctgcc gacaaggact gcagaccaca
     4141 caggctcact tggaaaggct cttgcaaaga gttttgcggg acaaagaggt ggccaatcgg
     4201 tacttcacca ctgtctgtgt gcggttactg cttgagagca aagaaaagaa aatcagggag
     4261 ttcattcaag actttcagaa acttactgct gctgatgata aaacggctca ggtggaggat
     4321 tttctacagt tcctgtatgg tgtgatggct caggatgtca tatggcagaa tgccagtgag
     4381 gagcagctgc aggatgccca gctggccatc gagcggagtg tgatgaatcg gatcttcaag
     4441 cttgctttct accctaacca ggatggggac atacttcgtg accaggttct gcatgaacat
     4501 atccagagat tatctaaagt agtgactgca aaccacagag ctctgcagat ccctgaggtt
     4561 tatctccgtg aggcaccatg gccatctgca cagtcagaaa tcaggacaat aagtgcttac
     4621 aaaacgcccc gcgacaaagt gcagtgcatc ctgaggatgt gctccaccat catgaacctc
     4681 ttgagcctgg ccaacgagga ctctgtcccc ggggcagatg actttgttcc tgtgttggtg
     4741 tttgtgttaa taaaggcaaa cccaccctgc ctgctgtcca ctgtgcagta catcagcagc
     4801 ttctatgcca gctgtctgtc tggagaggag tcctactggt ggatgcagtt cacagcagcc
     4861 gtggagttta ttaaaaccat cgatgacaga aagtgaccaa gaccaggccc accaagacag
     4921 cagactgtag ccaggaaaca gatctctagg aacatgtagc agcttctttg aaagctgaac
     4981 attgttttgt ctaatgctct acagcttcca gacgtttcca catggctctt cctaagcaga
     5041 ttaacaagct acacgcaggt cctcgttcct ttcggccttt ctgagttgca tattttattt
     5101 cctgacccca agtagagagt agtactgcaa aagggaccat gtttttcagg tgttttaaat
     5161 ttttaaaaac cagaacagaa atcttttagg aaacgtctag atcttggttc atgaattccc
     5221 tgttcgtcct tctcattttt agaaagaaca gtgtgcccct tccaagttgc tgtgtgagcg
     5281 ttcggtcgtg aggcacctgc acggagggtc tcaggagatg cgcaatacgg gctccctaga
     5341 tacactctcc taaggaagag ctaagaagga aatgtaaatc taatatatat ttatatttga
     5401 tgtactatgg gcatcttcag actctaataa acaacaagga cagttgctga gagtaggctg
     5461 tgacattccc tcttgtgaaa tggtttcctt gacaaaattt aagctgagct taaaagcaaa
     5521 agcagaaata tttattagtc tggagtagag tttatcgctc tgcatgtgtg gagtttgatg
     5581 gggaagtcat gaaggcgtgt accaccatgg acatgtgttg agcctgcgca ttggggcaga
     5641 ctccaccatg tggacaagca gtacgtggca tggcaggtgt gctcatgcgt ccttcctttg
     5701 cacctgcgca ctgagtggaa gccaggaagg gctccttacc gagcctcagc tatcatctgt
     5761 ctctgatgat ggttcacatt gaatgcacag accaggaatt cagtgaatgt cattttttaa
     5821 aactaatttg tatcgtctgc tctactgatc caggtcttac tagtgttaaa ctattttgat
     5881 cagccacact attcttatgg aaaaaaatct attttggcag gttcctgtgc ctttattgcc
     5941 ctcttctgaa aaaaaaaaaa aaaaaagtgt ctctgtagtt tggtttgcgt tgtacatcag
     6001 taattaatca ggaatgggct ttggtgcctg agaagctggc caaggaggca caccaaagct
     6061 tcaagcacaa gtcttgtaca tacgccatca ccgaggggtt tcaccttgta tgtttcctaa
     6121 acctgtctag cttttttgac aaatgctgca atcccatact tccgagtccc ttgtggtggc
     6181 agcaatacag gcatcttcta agggacccgt gacaaccagc catgagcagg tggataacga
     6241 ccgtctgctc cctctctaag aactcggcca caccgtgcca aaagaagctg agttttcttc
     6301 atgcttccac gtctcccttc cctttcaaat cccaaaatag gaatttacat aggaatgtga
     6361 gatgtggtac ctgcctttta gggagagatc aaaactaaac tagtatcaca aatatccgtt
     6421 cagatgttgg gtcaagaaat ctgtgtactt aatgggctgt cgttgtccac cttgccttgg
     6481 ggttggaagt cacatccctt gctgagtaca ttgcaagcca aactctgaca gggttctgca
     6541 actagaaggg agtctaacat ctacttcagc actgattcaa taaatgttaa gtcaacagaa
     6601 aattttgaat agttggaatt cattgttatg agacttaact tgtaaatcaa cgatctctct
     6661 ctctctctct ctctctctct ctctctctct gtaatggtag ctaaatatat tccatgttat
     6721 cactgtggaa ataaatatga agatgtgg
//
