LOCUS       NM_008085               1476 bp    mRNA    linear   ROD 01-AUG-2024
DEFINITION  Mus musculus glyceraldehyde-3-phosphate dehydrogenase,
            spermatogenic (Gapdhs), transcript variant 2, mRNA.
ACCESSION   NM_008085
VERSION     NM_008085.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Huang,Z., Danshina,P.V., Mohr,K., Qu,W., Goodson,S.G.,
            O'Connell,T.M. and O'Brien,D.A.
  TITLE     Sperm function, protein phosphorylation, and metabolism differ in
            mice lacking successive sperm-specific glycolytic enzymes
  JOURNAL   Biol Reprod 97 (4), 586-597 (2017)
   PUBMED   29025010
  REMARK    GeneRIF: These results identify multiple signaling and metabolic
            defects that are likely contributors to male infertility and the
            absence of progressive sperm motility seen in mice lacking GAPDHS.
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Bulusu,V., Prior,N., Snaebjornsson,M.T., Kuehne,A., Sonnen,K.F.,
            Kress,J., Stein,F., Schultz,C., Sauer,U. and Aulehla,A.
  TITLE     Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to
            Mouse Embryo Mesoderm Development
  JOURNAL   Dev Cell 40 (4), 331-341 (2017)
   PUBMED   28245920
REFERENCE   3  (bases 1 to 1476)
  AUTHORS   Bowman,C.E., Zhao,L., Hartung,T. and Wolfgang,M.J.
  TITLE     Requirement for the Mitochondrial Pyruvate Carrier in Mammalian
            Development Revealed by a Hypomorphic Allelic Series
  JOURNAL   Mol Cell Biol 36 (15), 2089-2104 (2016)
   PUBMED   27215380
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1476)
  AUTHORS   Chauvin,T., Xie,F., Liu,T., Nicora,C.D., Yang,F., Camp,D.G. 2nd,
            Smith,R.D. and Roberts,K.P.
  TITLE     A systematic analysis of a deep mouse epididymal sperm proteome
  JOURNAL   Biol Reprod 87 (6), 141 (2012)
   PUBMED   23115268
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1476)
  AUTHORS   Goodson,S.G., Zhang,Z., Tsuruta,J.K., Wang,W. and O'Brien,D.A.
  TITLE     Classification of mouse sperm motility patterns using an automated
            multiclass support vector machines model
  JOURNAL   Biol Reprod 84 (6), 1207-1215 (2011)
   PUBMED   21349820
REFERENCE   6  (bases 1 to 1476)
  AUTHORS   Miki,K., Qu,W., Goulding,E.H., Willis,W.D., Bunch,D.O.,
            Strader,L.F., Perreault,S.D., Eddy,E.M. and O'Brien,D.A.
  TITLE     Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific
            glycolytic enzyme, is required for sperm motility and male
            fertility
  JOURNAL   Proc Natl Acad Sci U S A 101 (47), 16501-16506 (2004)
   PUBMED   15546993
  REMARK    GeneRIF: Gapds(-/-) males were infertile and had profound defects
            in sperm motility, exhibiting sluggish movement without forward
            progression.
REFERENCE   7  (bases 1 to 1476)
  AUTHORS   Yang,J., Chennathukuzhi,V., Miki,K., O'Brien,D.A. and Hecht,N.B.
  TITLE     Mouse testis brain RNA-binding protein/translin selectively binds
            to the messenger RNA of the fibrous sheath protein glyceraldehyde
            3-phosphate dehydrogenase-S and suppresses its translation in vitro
  JOURNAL   Biol Reprod 68 (3), 853-859 (2003)
   PUBMED   12604635
  REMARK    GeneRIF: TB-RBP is involved in the posttranscriptional regulation
            of Gapds gene expression during spermiogenesis.
REFERENCE   8  (bases 1 to 1476)
  AUTHORS   Bunch,D.O., Welch,J.E., Magyar,P.L., Eddy,E.M. and O'Brien,D.A.
  TITLE     Glyceraldehyde 3-phosphate dehydrogenase-S protein distribution
            during mouse spermatogenesis
  JOURNAL   Biol Reprod 58 (3), 834-841 (1998)
   PUBMED   9510974
REFERENCE   9  (bases 1 to 1476)
  AUTHORS   Welch,J.E., Brown,P.R., O'Brien,D.A. and Eddy,E.M.
  TITLE     Genomic organization of a mouse glyceraldehyde 3-phosphate
            dehydrogenase gene (Gapd-s) expressed in post-meiotic spermatogenic
            cells
  JOURNAL   Dev Genet 16 (2), 179-189 (1995)
   PUBMED   7736666
REFERENCE   10 (bases 1 to 1476)
  AUTHORS   Welch,J.E., Schatte,E.C., O'Brien,D.A. and Eddy,E.M.
  TITLE     Expression of a glyceraldehyde 3-phosphate dehydrogenase gene
            specific to mouse spermatogenic cells
  JOURNAL   Biol Reprod 46 (5), 869-878 (1992)
   PUBMED   1375514
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC167978.4.
            
            On Mar 15, 2014 this sequence version replaced NM_008085.1.
            
            Transcript Variant: This variant (2) contains an alternate first
            exon compared to variant 1. The resulting isoform (2) has a shorter
            and distinct N-terminus compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849384
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-148               AC167978.4         15592-15739
            149-386             AC167978.4         16964-17201
            387-483             AC167978.4         17980-18076
            484-590             AC167978.4         18274-18380
            591-681             AC167978.4         21657-21747
            682-800             AC167978.4         21829-21947
            801-882             AC167978.4         22306-22387
            883-1034            AC167978.4         22652-22803
            1035-1197           AC167978.4         22935-23097
            1198-1295           AC167978.4         24567-24664
            1296-1476           AC167978.4         25040-25220
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 19.23 cM"
     gene            1..1476
                     /gene="Gapdhs"
                     /gene_synonym="Gapd-s; Gapds"
                     /note="glyceraldehyde-3-phosphate dehydrogenase,
                     spermatogenic"
                     /db_xref="GeneID:14447"
                     /db_xref="MGI:MGI:95653"
     exon            1..148
                     /gene="Gapdhs"
                     /gene_synonym="Gapd-s; Gapds"
                     /inference="alignment:Splign:2.1.0"
     CDS             52..1368
                     /gene="Gapdhs"
                     /gene_synonym="Gapd-s; Gapds"
                     /EC_number="1.2.1.12"
                     /note="isoform 2 is encoded by transcript variant 2;
                     glyceraldehyde-3-phosphate dehydrogenase, testis-specific;
                     GAPDH-2; spermatogenic glyceraldehyde-3-phosphate
                     dehydrogenase; spermatogenic cell-specific glyceraldehyde
                     3-phosphate dehydrogenase 2"
                     /codon_start=1
                     /product="glyceraldehyde-3-phosphate dehydrogenase,
                     testis-specific isoform 2"
                     /protein_id="NP_032111.1"
                     /db_xref="CCDS:CCDS21109.1"
                     /db_xref="GeneID:14447"
                     /db_xref="MGI:MGI:95653"
                     /translation="MSRRDVVLTNVTVVQLRRDRCPCPCPCPCPCPVIRPPPPKVEDP
                     PPTVEEQPPPPPPPPPPPPPPPPPPPPQIEPDKFEEAPPPPPPPPPPPPPPPPPLQKP
                     ARELTVGINGFGRIGRLVLRVCMEKGIRVVAVNDPFIDPEYMVYMFKYDSTHGRYKGN
                     VEHKNGQLVVDNLEINTYQCKDPKEIPWSSIGNPYVVECTGVYLSIEAASAHISSGAR
                     RVVVTAPSPDAPMFVMGVNEKDYNPGSMTIVSNASCTTNCLAPLAKVIHENFGIVEGL
                     MTTVHSYTATQKTVDGPSKKDWRGGRGAHQNIIPSSTGAAKAVGKVIPELKGKLTGMA
                     FRVPTPNVSVVDLTCRLAKPASYSAITEAVKAAAKGPLAGILAYTEDQVVSTDFNGNP
                     HSSIFDAKAGIALNDNFVKLVAWYDNEYGYSNRVVDLLRYMFSREK"
     exon            149..386
                     /gene="Gapdhs"
                     /gene_synonym="Gapd-s; Gapds"
                     /inference="alignment:Splign:2.1.0"
     exon            387..483
                     /gene="Gapdhs"
                     /gene_synonym="Gapd-s; Gapds"
                     /inference="alignment:Splign:2.1.0"
     exon            484..590
                     /gene="Gapdhs"
                     /gene_synonym="Gapd-s; Gapds"
                     /inference="alignment:Splign:2.1.0"
     exon            591..681
                     /gene="Gapdhs"
                     /gene_synonym="Gapd-s; Gapds"
                     /inference="alignment:Splign:2.1.0"
     exon            682..800
                     /gene="Gapdhs"
                     /gene_synonym="Gapd-s; Gapds"
                     /inference="alignment:Splign:2.1.0"
     exon            801..882
                     /gene="Gapdhs"
                     /gene_synonym="Gapd-s; Gapds"
                     /inference="alignment:Splign:2.1.0"
     exon            883..1034
                     /gene="Gapdhs"
                     /gene_synonym="Gapd-s; Gapds"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1197
                     /gene="Gapdhs"
                     /gene_synonym="Gapd-s; Gapds"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1295
                     /gene="Gapdhs"
                     /gene_synonym="Gapd-s; Gapds"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1476
                     /gene="Gapdhs"
                     /gene_synonym="Gapd-s; Gapds"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtaacacca cggagggggg ccaaggcagc caggccatga gatcttaggc catgtcgaga
       61 cgtgacgtgg tccttaccaa tgttactgtt gtccagctac ggcgggaccg atgcccatgc
      121 ccatgcccat gtccatgccc atgccctgtg atcagaccac ctccacccaa ggttgaggat
      181 ccaccaccca cggttgaaga acagccaccg ccaccgccgc cgccacctcc acctccacca
      241 ccacctcctc ctcctcctcc accccagata gagccagaca agtttgaaga ggctccccct
      301 ccccctcccc ctcctcctcc tcctccccct ccccctcccc caccactcca aaagccagct
      361 agagagctga cagtgggtat caatggattt ggacgcattg gtcgtctggt gctgcgagtc
      421 tgcatggaga agggcattag ggtggtagca gtgaatgacc cattcattga tccagaatac
      481 atggtttaca tgttcaaata tgactccaca catggtagat acaaaggaaa cgtggaacat
      541 aagaatggac aactagttgt ggacaacctt gagatcaaca cgtaccagtg caaagaccct
      601 aaagaaatcc cctggagctc tatagggaat ccctacgtgg tggagtgtac aggcgtctat
      661 ctgtccatcg aggcagcttc ggcacatatt tcatctggtg ccaggcgtgt ggtggtcact
      721 gcaccctccc ccgatgcacc catgtttgtc atgggagtga acgagaagga ctataaccct
      781 ggctctatga ccattgtcag caatgcatcc tgtaccacca actgcctggc tcctctcgcc
      841 aaggttattc atgaaaactt cgggatcgtg gaagggctaa tgaccacagt ccattcctac
      901 acagccactc agaagacagt ggatgggcca tcaaagaagg actggcgagg tggccgcggc
      961 gctcaccaaa acatcatccc atcgtccact ggggctgcca aggctgtagg caaagtcatc
     1021 ccagagctca aagggaagct aacaggaatg gcattccggg tgccaacccc aaacgtgtca
     1081 gttgtggacc tgacctgccg cctggccaag cctgcttctt actcggctat cacggaggct
     1141 gtgaaagctg cagccaaggg acctttggct ggcatccttg cttacacaga ggaccaggtg
     1201 gtctccacgg actttaacgg caatccccat tcttccatct ttgatgctaa ggctggaatt
     1261 gccctcaatg acaacttcgt gaagcttgtt gcctggtacg acaacgaata tggctacagt
     1321 aaccgagtgg tcgacctcct ccgctacatg tttagccgag agaagtaaca caaaaggccc
     1381 ctccttgctc ccctgcgcac ctcgcgttcc tgacttcggc ttccactcaa aggcgccgcc
     1441 accgggtcaa caatgaaata aaaacgagaa tgcgca
//
