LOCUS       NP_032111                438 aa            linear   ROD 01-AUG-2024
DEFINITION  glyceraldehyde-3-phosphate dehydrogenase, testis-specific isoform 2
            [Mus musculus].
ACCESSION   NP_032111
VERSION     NP_032111.1
DBSOURCE    REFSEQ: accession NM_008085.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 438)
  AUTHORS   Huang,Z., Danshina,P.V., Mohr,K., Qu,W., Goodson,S.G.,
            O'Connell,T.M. and O'Brien,D.A.
  TITLE     Sperm function, protein phosphorylation, and metabolism differ in
            mice lacking successive sperm-specific glycolytic enzymes
  JOURNAL   Biol Reprod 97 (4), 586-597 (2017)
   PUBMED   29025010
  REMARK    GeneRIF: These results identify multiple signaling and metabolic
            defects that are likely contributors to male infertility and the
            absence of progressive sperm motility seen in mice lacking GAPDHS.
REFERENCE   2  (residues 1 to 438)
  AUTHORS   Bulusu,V., Prior,N., Snaebjornsson,M.T., Kuehne,A., Sonnen,K.F.,
            Kress,J., Stein,F., Schultz,C., Sauer,U. and Aulehla,A.
  TITLE     Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to
            Mouse Embryo Mesoderm Development
  JOURNAL   Dev Cell 40 (4), 331-341 (2017)
   PUBMED   28245920
REFERENCE   3  (residues 1 to 438)
  AUTHORS   Bowman,C.E., Zhao,L., Hartung,T. and Wolfgang,M.J.
  TITLE     Requirement for the Mitochondrial Pyruvate Carrier in Mammalian
            Development Revealed by a Hypomorphic Allelic Series
  JOURNAL   Mol Cell Biol 36 (15), 2089-2104 (2016)
   PUBMED   27215380
  REMARK    Publication Status: Online-Only
REFERENCE   4  (residues 1 to 438)
  AUTHORS   Chauvin,T., Xie,F., Liu,T., Nicora,C.D., Yang,F., Camp,D.G. 2nd,
            Smith,R.D. and Roberts,K.P.
  TITLE     A systematic analysis of a deep mouse epididymal sperm proteome
  JOURNAL   Biol Reprod 87 (6), 141 (2012)
   PUBMED   23115268
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 438)
  AUTHORS   Goodson,S.G., Zhang,Z., Tsuruta,J.K., Wang,W. and O'Brien,D.A.
  TITLE     Classification of mouse sperm motility patterns using an automated
            multiclass support vector machines model
  JOURNAL   Biol Reprod 84 (6), 1207-1215 (2011)
   PUBMED   21349820
REFERENCE   6  (residues 1 to 438)
  AUTHORS   Miki,K., Qu,W., Goulding,E.H., Willis,W.D., Bunch,D.O.,
            Strader,L.F., Perreault,S.D., Eddy,E.M. and O'Brien,D.A.
  TITLE     Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific
            glycolytic enzyme, is required for sperm motility and male
            fertility
  JOURNAL   Proc Natl Acad Sci U S A 101 (47), 16501-16506 (2004)
   PUBMED   15546993
  REMARK    GeneRIF: Gapds(-/-) males were infertile and had profound defects
            in sperm motility, exhibiting sluggish movement without forward
            progression.
REFERENCE   7  (residues 1 to 438)
  AUTHORS   Yang,J., Chennathukuzhi,V., Miki,K., O'Brien,D.A. and Hecht,N.B.
  TITLE     Mouse testis brain RNA-binding protein/translin selectively binds
            to the messenger RNA of the fibrous sheath protein glyceraldehyde
            3-phosphate dehydrogenase-S and suppresses its translation in vitro
  JOURNAL   Biol Reprod 68 (3), 853-859 (2003)
   PUBMED   12604635
  REMARK    GeneRIF: TB-RBP is involved in the posttranscriptional regulation
            of Gapds gene expression during spermiogenesis.
REFERENCE   8  (residues 1 to 438)
  AUTHORS   Bunch,D.O., Welch,J.E., Magyar,P.L., Eddy,E.M. and O'Brien,D.A.
  TITLE     Glyceraldehyde 3-phosphate dehydrogenase-S protein distribution
            during mouse spermatogenesis
  JOURNAL   Biol Reprod 58 (3), 834-841 (1998)
   PUBMED   9510974
REFERENCE   9  (residues 1 to 438)
  AUTHORS   Welch,J.E., Brown,P.R., O'Brien,D.A. and Eddy,E.M.
  TITLE     Genomic organization of a mouse glyceraldehyde 3-phosphate
            dehydrogenase gene (Gapd-s) expressed in post-meiotic spermatogenic
            cells
  JOURNAL   Dev Genet 16 (2), 179-189 (1995)
   PUBMED   7736666
REFERENCE   10 (residues 1 to 438)
  AUTHORS   Welch,J.E., Schatte,E.C., O'Brien,D.A. and Eddy,E.M.
  TITLE     Expression of a glyceraldehyde 3-phosphate dehydrogenase gene
            specific to mouse spermatogenic cells
  JOURNAL   Biol Reprod 46 (5), 869-878 (1992)
   PUBMED   1375514
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC167978.4.
            
            Transcript Variant: This variant (2) contains an alternate first
            exon compared to variant 1. The resulting isoform (2) has a shorter
            and distinct N-terminus compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849384
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 19.23 cM"
     Protein         1..438
                     /product="glyceraldehyde-3-phosphate dehydrogenase,
                     testis-specific isoform 2"
                     /EC_number="1.2.1.12"
                     /note="glyceraldehyde-3-phosphate dehydrogenase,
                     testis-specific; GAPDH-2; spermatogenic
                     glyceraldehyde-3-phosphate dehydrogenase; spermatogenic
                     cell-specific glyceraldehyde 3-phosphate dehydrogenase 2"
                     /calculated_mol_wt=47312
     Region          38..104
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q64467.1)"
     Region          108..433
                     /region_name="GapA"
                     /note="Glyceraldehyde-3-phosphate
                     dehydrogenase/erythrose-4-phosphate dehydrogenase
                     [Carbohydrate transport and metabolism]; COG0057"
                     /db_xref="CDD:439827"
     Site            281
                     /site_type="other"
                     /note="Activates thiol group during catalysis.
                     /evidence=ECO:0000250; propagated from
                     UniProtKB/Swiss-Prot (Q64467.1)"
     Site            356
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9ESV6; propagated from
                     UniProtKB/Swiss-Prot (Q64467.1)"
     CDS             1..438
                     /gene="Gapdhs"
                     /gene_synonym="Gapd-s; Gapds"
                     /coded_by="NM_008085.2:52..1368"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /db_xref="CCDS:CCDS21109.1"
                     /db_xref="GeneID:14447"
                     /db_xref="MGI:MGI:95653"
ORIGIN      
        1 msrrdvvltn vtvvqlrrdr cpcpcpcpcp cpvirppppk vedppptvee qppppppppp
       61 pppppppppp pqiepdkfee appppppppp pppppppplq kpareltvgi ngfgrigrlv
      121 lrvcmekgir vvavndpfid peymvymfky dsthgrykgn vehkngqlvv dnleintyqc
      181 kdpkeipwss ignpyvvect gvylsieaas ahissgarrv vvtapspdap mfvmgvnekd
      241 ynpgsmtivs nascttncla plakvihenf giveglmttv hsytatqktv dgpskkdwrg
      301 grgahqniip sstgaakavg kvipelkgkl tgmafrvptp nvsvvdltcr lakpasysai
      361 teavkaaakg plagilayte dqvvstdfng nphssifdak agialndnfv klvawydney
      421 gysnrvvdll rymfsrek
//
