LOCUS       NM_001411844            1389 bp    mRNA    linear   ROD 07-DEC-2024
DEFINITION  Mus musculus glyceraldehyde-3-phosphate dehydrogenase (Gapdh),
            transcript variant 7, mRNA.
ACCESSION   NM_001411844
VERSION     NM_001411844.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Ling,L., Luo,M., Yin,H., Tian,Y., Wang,T., Zhang,B., Yin,L.,
            Zhang,Y. and Bian,J.
  TITLE     Sinomenine Ameliorated Microglial Activation and Neuropathic Pain
            After Chronic Constriction Injury Via TGF-beta1/ALK5/Smad3
            Signalling Pathway
  JOURNAL   J Cell Mol Med 28 (22), e70214 (2024)
   PUBMED   39586784
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Ayyangar,U., Karkhanis,A., Tay,H., Afandi,A.F.B., Bhattacharjee,O.,
            Ks,L., Lee,S.H., Chan,J. and Raghavan,S.
  TITLE     Metabolic rewiring of macrophages by epidermal-derived lactate
            promotes sterile inflammation in the murine skin
  JOURNAL   EMBO J 43 (7), 1113-1134 (2024)
   PUBMED   38418556
REFERENCE   3  (bases 1 to 1389)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 1389)
  AUTHORS   Sharma,S., Kajjo,S., Harra,Z., Hasaj,B., Delisle,V., Ray,D.,
            Gutierrez,R.L., Carrier,I., Kleinman,C., Morris,Q., Hughes,T.R.,
            McInnes,R. and Fabian,M.R.
  TITLE     Uncovering a mammalian neural-specific poly(A) binding protein with
            unique properties
  JOURNAL   Genes Dev 37 (15-16), 760-777 (2023)
   PUBMED   37704377
REFERENCE   5  (bases 1 to 1389)
  AUTHORS   Kornberg,M.D., Sen,N., Hara,M.R., Juluri,K.R., Nguyen,J.V.,
            Snowman,A.M., Law,L., Hester,L.D. and Snyder,S.H.
  TITLE     GAPDH mediates nitrosylation of nuclear proteins
  JOURNAL   Nat Cell Biol 12 (11), 1094-1100 (2010)
   PUBMED   20972425
  REMARK    GeneRIF: Gapdh is a moonlighting protein that functions as a
            glycolytic enzyme and a nuclear protein nitrosylase.
REFERENCE   6  (bases 1 to 1389)
  AUTHORS   Rodriguez-Pascual,F., Redondo-Horcajo,M., Magan-Marchal,N.,
            Lagares,D., Martinez-Ruiz,A., Kleinert,H. and Lamas,S.
  TITLE     Glyceraldehyde-3-phosphate dehydrogenase regulates endothelin-1
            expression by a novel, redox-sensitive mechanism involving mRNA
            stability
  JOURNAL   Mol Cell Biol 28 (23), 7139-7155 (2008)
   PUBMED   18809573
  REMARK    GeneRIF: Gapdh is a moonlighting protein that functions as a
            glycolytic enzyme and additionally is involved in regulating mRNA
            stability.
REFERENCE   7  (bases 1 to 1389)
  AUTHORS   Raje,C.I., Kumar,S., Harle,A., Nanda,J.S. and Raje,M.
  TITLE     The macrophage cell surface glyceraldehyde-3-phosphate
            dehydrogenase is a novel transferrin receptor
  JOURNAL   J Biol Chem 282 (5), 3252-3261 (2007)
   PUBMED   17121833
  REMARK    GeneRIF: GAPDH interacts with transferrin and the GAPDH-transferrin
            complex is subsequently internalized into the early endosomes.
            GeneRIF: Gapdh is a moonlighting protein that functions as a
            glycolytic enzyme and a transferrin receptor.
REFERENCE   8  (bases 1 to 1389)
  AUTHORS   Jones,C.M., Simon-Chazottes,D., Guenet,J.L. and Hogan,B.L.
  TITLE     Isolation of Vgr-2, a novel member of the transforming growth
            factor-beta-related gene family
  JOURNAL   Mol Endocrinol 6 (11), 1961-1968 (1992)
   PUBMED   1480182
REFERENCE   9  (bases 1 to 1389)
  AUTHORS   Welch,J.E., Schatte,E.C., O'Brien,D.A. and Eddy,E.M.
  TITLE     Expression of a glyceraldehyde 3-phosphate dehydrogenase gene
            specific to mouse spermatogenic cells
  JOURNAL   Biol Reprod 46 (5), 869-878 (1992)
   PUBMED   1375514
REFERENCE   10 (bases 1 to 1389)
  AUTHORS   Bruns,G.A. and Gerald,P.S.
  TITLE     Human glyceraldehyde-3-phosphate dehydrogenase in man-rodent
            somatic cell hybrids
  JOURNAL   Science 192 (4234), 54-56 (1976)
   PUBMED   176725
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166162.6.
            
            Summary: This gene encodes a member of the
            glyceraldehyde-3-phosphate dehydrogenase protein family. The
            encoded protein has been identified as a moonlighting protein based
            on its ability to perform mechanistically distinct functions. The
            encoded protein was originally identified as a key glycolytic
            enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into
            3-phospho-D-glyceroyl phosphate. Subsequent studies have assigned a
            variety of additional functions to the protein including
            nitrosylation of nuclear proteins, the regulation of mRNA
            stability, and acting as a transferrin receptor on the cell surface
            of macrophage. Alternative splicing results in multiple transcript
            variants. Many pseudogenes similar to this locus are found
            throughout the mouse genome. [provided by RefSeq, Jan 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17784647.1352151.1,
                                           SRR17784650.837872.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            multifunctional gene product(s) :: PMID: 17121833, 18809573,
                                               20972425
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-227               AC166162.6         138418-138644       c
            228-525             AC166162.6         136460-136757       c
            526-723             AC166162.6         136164-136361       c
            724-954             AC166162.6         135799-136029       c
            955-1136            AC166162.6         135533-135714       c
            1137-1389           AC166162.6         135170-135422       c
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 59.32 cM"
     gene            1..1389
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /note="glyceraldehyde-3-phosphate dehydrogenase"
                     /db_xref="GeneID:14433"
                     /db_xref="MGI:MGI:95640"
     exon            1..227
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     exon            228..525
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     CDS             325..1206
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /EC_number="1.2.1.12"
                     /note="isoform 3 is encoded by transcript variant 7;
                     peptidyl-cysteine S-nitrosylase GAPDH"
                     /codon_start=1
                     /product="glyceraldehyde-3-phosphate dehydrogenase isoform
                     3"
                     /protein_id="NP_001398773.1"
                     /db_xref="GeneID:14433"
                     /db_xref="MGI:MGI:95640"
                     /translation="MVYMFQYDSTHGKFNGTVKAENGKLVINGKPITIFQERDPTNIK
                     WGEAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVNHEKYDNSL
                     KIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGA
                     AQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKK
                     VVKQASEGPLKGILGYTEDQVVSCDFNSNSHSSTFDAGAGIALNDNFVKLISWYDNEY
                     GYSNRVVDLMAYMASKE"
     exon            526..723
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     exon            724..954
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1136
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     exon            1137..1389
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1366..1371
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /note="hexamer: AATAAA"
     polyA_site      1389
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /note="major polyA site"
ORIGIN      
        1 cttctcctgc agcctcgtcc cgtagacaaa atggtgaagg tcggtgtgaa cgggtgagtt
       61 ccagggcggg gccctgctcc gttgcctacg caggtcttgc tgacccgggg gctctgcagt
      121 actgtgggga ggtggatgag gtggccgaag cgcccaagga gacctcaagg tcagcgctcg
      181 gacctggcga tggctcgcac ttgcggcccc cagctgctgc acctctgatt tggccgtatt
      241 gggcgcctgg tcaccagggc tgccatttgc agtggcaaag tggagattgt tgccatcaac
      301 gaccccttca ttgacctcaa ctacatggtc tacatgttcc agtatgactc cactcacggc
      361 aaattcaacg gcacagtcaa ggccgagaat gggaagcttg tcatcaacgg gaagcccatc
      421 accatcttcc aggagcgaga ccccactaac atcaaatggg gtgaggccgg tgctgagtat
      481 gtcgtggagt ctactggtgt cttcaccacc atggagaagg ccggggccca cttgaagggt
      541 ggagccaaaa gggtcatcat ctccgcccct tctgccgatg cccccatgtt tgtgatgggt
      601 gtgaaccacg agaaatatga caactcactc aagattgtca gcaatgcatc ctgcaccacc
      661 aactgcttag cccccctggc caaggtcatc catgacaact ttggcattgt ggaagggctc
      721 atgaccacag tccatgccat cactgccacc cagaagactg tggatggccc ctctggaaag
      781 ctgtggcgtg atggccgtgg ggctgcccag aacatcatcc ctgcatccac tggtgctgcc
      841 aaggctgtgg gcaaggtcat cccagagctg aacgggaagc tcactggcat ggccttccgt
      901 gttcctaccc ccaatgtgtc cgtcgtggat ctgacgtgcc gcctggagaa acctgccaag
      961 tatgatgaca tcaagaaggt ggtgaagcag gcatctgagg gcccactgaa gggcatcttg
     1021 ggctacactg aggaccaggt tgtctcctgc gacttcaaca gcaactccca ctcttccacc
     1081 ttcgatgccg gggctggcat tgctctcaat gacaactttg tcaagctcat ttcctggtat
     1141 gacaatgaat acggctacag caacagggtg gtggacctca tggcctacat ggcctccaag
     1201 gagtaagaaa ccctggacca cccaccccag caaggacact gagcaagaga ggccctatcc
     1261 caactcggcc cccaacactg agcatctccc tcacaatttc catcccagac ccccataata
     1321 acaggagggg cctagggagc cctccctact ctcttgaata ccatcaataa agttcgctgc
     1381 acccacctc
//
