LOCUS       NM_001411841            1373 bp    mRNA    linear   ROD 07-DEC-2024
DEFINITION  Mus musculus glyceraldehyde-3-phosphate dehydrogenase (Gapdh),
            transcript variant 4, mRNA.
ACCESSION   NM_001411841
VERSION     NM_001411841.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Ling,L., Luo,M., Yin,H., Tian,Y., Wang,T., Zhang,B., Yin,L.,
            Zhang,Y. and Bian,J.
  TITLE     Sinomenine Ameliorated Microglial Activation and Neuropathic Pain
            After Chronic Constriction Injury Via TGF-beta1/ALK5/Smad3
            Signalling Pathway
  JOURNAL   J Cell Mol Med 28 (22), e70214 (2024)
   PUBMED   39586784
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Ayyangar,U., Karkhanis,A., Tay,H., Afandi,A.F.B., Bhattacharjee,O.,
            Ks,L., Lee,S.H., Chan,J. and Raghavan,S.
  TITLE     Metabolic rewiring of macrophages by epidermal-derived lactate
            promotes sterile inflammation in the murine skin
  JOURNAL   EMBO J 43 (7), 1113-1134 (2024)
   PUBMED   38418556
REFERENCE   3  (bases 1 to 1373)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 1373)
  AUTHORS   Sharma,S., Kajjo,S., Harra,Z., Hasaj,B., Delisle,V., Ray,D.,
            Gutierrez,R.L., Carrier,I., Kleinman,C., Morris,Q., Hughes,T.R.,
            McInnes,R. and Fabian,M.R.
  TITLE     Uncovering a mammalian neural-specific poly(A) binding protein with
            unique properties
  JOURNAL   Genes Dev 37 (15-16), 760-777 (2023)
   PUBMED   37704377
REFERENCE   5  (bases 1 to 1373)
  AUTHORS   Kornberg,M.D., Sen,N., Hara,M.R., Juluri,K.R., Nguyen,J.V.,
            Snowman,A.M., Law,L., Hester,L.D. and Snyder,S.H.
  TITLE     GAPDH mediates nitrosylation of nuclear proteins
  JOURNAL   Nat Cell Biol 12 (11), 1094-1100 (2010)
   PUBMED   20972425
  REMARK    GeneRIF: Gapdh is a moonlighting protein that functions as a
            glycolytic enzyme and a nuclear protein nitrosylase.
REFERENCE   6  (bases 1 to 1373)
  AUTHORS   Rodriguez-Pascual,F., Redondo-Horcajo,M., Magan-Marchal,N.,
            Lagares,D., Martinez-Ruiz,A., Kleinert,H. and Lamas,S.
  TITLE     Glyceraldehyde-3-phosphate dehydrogenase regulates endothelin-1
            expression by a novel, redox-sensitive mechanism involving mRNA
            stability
  JOURNAL   Mol Cell Biol 28 (23), 7139-7155 (2008)
   PUBMED   18809573
  REMARK    GeneRIF: Gapdh is a moonlighting protein that functions as a
            glycolytic enzyme and additionally is involved in regulating mRNA
            stability.
REFERENCE   7  (bases 1 to 1373)
  AUTHORS   Raje,C.I., Kumar,S., Harle,A., Nanda,J.S. and Raje,M.
  TITLE     The macrophage cell surface glyceraldehyde-3-phosphate
            dehydrogenase is a novel transferrin receptor
  JOURNAL   J Biol Chem 282 (5), 3252-3261 (2007)
   PUBMED   17121833
  REMARK    GeneRIF: GAPDH interacts with transferrin and the GAPDH-transferrin
            complex is subsequently internalized into the early endosomes.
            GeneRIF: Gapdh is a moonlighting protein that functions as a
            glycolytic enzyme and a transferrin receptor.
REFERENCE   8  (bases 1 to 1373)
  AUTHORS   Jones,C.M., Simon-Chazottes,D., Guenet,J.L. and Hogan,B.L.
  TITLE     Isolation of Vgr-2, a novel member of the transforming growth
            factor-beta-related gene family
  JOURNAL   Mol Endocrinol 6 (11), 1961-1968 (1992)
   PUBMED   1480182
REFERENCE   9  (bases 1 to 1373)
  AUTHORS   Welch,J.E., Schatte,E.C., O'Brien,D.A. and Eddy,E.M.
  TITLE     Expression of a glyceraldehyde 3-phosphate dehydrogenase gene
            specific to mouse spermatogenic cells
  JOURNAL   Biol Reprod 46 (5), 869-878 (1992)
   PUBMED   1375514
REFERENCE   10 (bases 1 to 1373)
  AUTHORS   Bruns,G.A. and Gerald,P.S.
  TITLE     Human glyceraldehyde-3-phosphate dehydrogenase in man-rodent
            somatic cell hybrids
  JOURNAL   Science 192 (4234), 54-56 (1976)
   PUBMED   176725
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166162.6.
            
            Summary: This gene encodes a member of the
            glyceraldehyde-3-phosphate dehydrogenase protein family. The
            encoded protein has been identified as a moonlighting protein based
            on its ability to perform mechanistically distinct functions. The
            encoded protein was originally identified as a key glycolytic
            enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into
            3-phospho-D-glyceroyl phosphate. Subsequent studies have assigned a
            variety of additional functions to the protein including
            nitrosylation of nuclear proteins, the regulation of mRNA
            stability, and acting as a transferrin receptor on the cell surface
            of macrophage. Alternative splicing results in multiple transcript
            variants. Many pseudogenes similar to this locus are found
            throughout the mouse genome. [provided by RefSeq, Jan 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.585758.1,
                                           SRR7345562.1962987.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            multifunctional gene product(s) :: PMID: 17121833, 18809573,
                                               20972425
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-54                AC166162.6         138873-138926       c
            55-211              AC166162.6         138476-138632       c
            212-509             AC166162.6         136460-136757       c
            510-707             AC166162.6         136164-136361       c
            708-938             AC166162.6         135799-136029       c
            939-1120            AC166162.6         135533-135714       c
            1121-1373           AC166162.6         135170-135422       c
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 59.32 cM"
     gene            1..1373
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /note="glyceraldehyde-3-phosphate dehydrogenase"
                     /db_xref="GeneID:14433"
                     /db_xref="MGI:MGI:95640"
     exon            1..54
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     exon            55..211
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     exon            212..509
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     CDS             309..1190
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /EC_number="1.2.1.12"
                     /note="isoform 3 is encoded by transcript variant 4;
                     peptidyl-cysteine S-nitrosylase GAPDH"
                     /codon_start=1
                     /product="glyceraldehyde-3-phosphate dehydrogenase isoform
                     3"
                     /protein_id="NP_001398770.1"
                     /db_xref="GeneID:14433"
                     /db_xref="MGI:MGI:95640"
                     /translation="MVYMFQYDSTHGKFNGTVKAENGKLVINGKPITIFQERDPTNIK
                     WGEAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVNHEKYDNSL
                     KIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGA
                     AQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKK
                     VVKQASEGPLKGILGYTEDQVVSCDFNSNSHSSTFDAGAGIALNDNFVKLISWYDNEY
                     GYSNRVVDLMAYMASKE"
     exon            510..707
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     exon            708..938
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     exon            939..1120
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     exon            1121..1373
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1350..1355
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /note="hexamer: AATAAA"
     polyA_site      1373
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /note="major polyA site"
ORIGIN      
        1 gctctctgct cctccctgtt ccagagacgg ccgcatcttc ttgtgcagtg ccagcctcgt
       61 cccgtagaca aaatggtgaa ggtcggtgtg aacgggtgag ttccagggcg gggccctgct
      121 ccgttgccta cgcaggtctt gctgacccgg gggctctgca gtactgtggg gaggtggatg
      181 aggtggccga agcgcccaag gagacctcaa gatttggccg tattgggcgc ctggtcacca
      241 gggctgccat ttgcagtggc aaagtggaga ttgttgccat caacgacccc ttcattgacc
      301 tcaactacat ggtctacatg ttccagtatg actccactca cggcaaattc aacggcacag
      361 tcaaggccga gaatgggaag cttgtcatca acgggaagcc catcaccatc ttccaggagc
      421 gagaccccac taacatcaaa tggggtgagg ccggtgctga gtatgtcgtg gagtctactg
      481 gtgtcttcac caccatggag aaggccgggg cccacttgaa gggtggagcc aaaagggtca
      541 tcatctccgc cccttctgcc gatgccccca tgtttgtgat gggtgtgaac cacgagaaat
      601 atgacaactc actcaagatt gtcagcaatg catcctgcac caccaactgc ttagcccccc
      661 tggccaaggt catccatgac aactttggca ttgtggaagg gctcatgacc acagtccatg
      721 ccatcactgc cacccagaag actgtggatg gcccctctgg aaagctgtgg cgtgatggcc
      781 gtggggctgc ccagaacatc atccctgcat ccactggtgc tgccaaggct gtgggcaagg
      841 tcatcccaga gctgaacggg aagctcactg gcatggcctt ccgtgttcct acccccaatg
      901 tgtccgtcgt ggatctgacg tgccgcctgg agaaacctgc caagtatgat gacatcaaga
      961 aggtggtgaa gcaggcatct gagggcccac tgaagggcat cttgggctac actgaggacc
     1021 aggttgtctc ctgcgacttc aacagcaact cccactcttc caccttcgat gccggggctg
     1081 gcattgctct caatgacaac tttgtcaagc tcatttcctg gtatgacaat gaatacggct
     1141 acagcaacag ggtggtggac ctcatggcct acatggcctc caaggagtaa gaaaccctgg
     1201 accacccacc ccagcaagga cactgagcaa gagaggccct atcccaactc ggcccccaac
     1261 actgagcatc tccctcacaa tttccatccc agacccccat aataacagga ggggcctagg
     1321 gagccctccc tactctcttg aataccatca ataaagttcg ctgcacccac ctc
//
