LOCUS       NM_001289726            1297 bp    mRNA    linear   ROD 07-DEC-2024
DEFINITION  Mus musculus glyceraldehyde-3-phosphate dehydrogenase (Gapdh),
            transcript variant 1, mRNA.
ACCESSION   NM_001289726
VERSION     NM_001289726.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Ling,L., Luo,M., Yin,H., Tian,Y., Wang,T., Zhang,B., Yin,L.,
            Zhang,Y. and Bian,J.
  TITLE     Sinomenine Ameliorated Microglial Activation and Neuropathic Pain
            After Chronic Constriction Injury Via TGF-beta1/ALK5/Smad3
            Signalling Pathway
  JOURNAL   J Cell Mol Med 28 (22), e70214 (2024)
   PUBMED   39586784
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Ayyangar,U., Karkhanis,A., Tay,H., Afandi,A.F.B., Bhattacharjee,O.,
            Ks,L., Lee,S.H., Chan,J. and Raghavan,S.
  TITLE     Metabolic rewiring of macrophages by epidermal-derived lactate
            promotes sterile inflammation in the murine skin
  JOURNAL   EMBO J 43 (7), 1113-1134 (2024)
   PUBMED   38418556
REFERENCE   3  (bases 1 to 1297)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 1297)
  AUTHORS   Sharma,S., Kajjo,S., Harra,Z., Hasaj,B., Delisle,V., Ray,D.,
            Gutierrez,R.L., Carrier,I., Kleinman,C., Morris,Q., Hughes,T.R.,
            McInnes,R. and Fabian,M.R.
  TITLE     Uncovering a mammalian neural-specific poly(A) binding protein with
            unique properties
  JOURNAL   Genes Dev 37 (15-16), 760-777 (2023)
   PUBMED   37704377
REFERENCE   5  (bases 1 to 1297)
  AUTHORS   Kornberg,M.D., Sen,N., Hara,M.R., Juluri,K.R., Nguyen,J.V.,
            Snowman,A.M., Law,L., Hester,L.D. and Snyder,S.H.
  TITLE     GAPDH mediates nitrosylation of nuclear proteins
  JOURNAL   Nat Cell Biol 12 (11), 1094-1100 (2010)
   PUBMED   20972425
  REMARK    GeneRIF: Gapdh is a moonlighting protein that functions as a
            glycolytic enzyme and a nuclear protein nitrosylase.
REFERENCE   6  (bases 1 to 1297)
  AUTHORS   Rodriguez-Pascual,F., Redondo-Horcajo,M., Magan-Marchal,N.,
            Lagares,D., Martinez-Ruiz,A., Kleinert,H. and Lamas,S.
  TITLE     Glyceraldehyde-3-phosphate dehydrogenase regulates endothelin-1
            expression by a novel, redox-sensitive mechanism involving mRNA
            stability
  JOURNAL   Mol Cell Biol 28 (23), 7139-7155 (2008)
   PUBMED   18809573
  REMARK    GeneRIF: Gapdh is a moonlighting protein that functions as a
            glycolytic enzyme and additionally is involved in regulating mRNA
            stability.
REFERENCE   7  (bases 1 to 1297)
  AUTHORS   Raje,C.I., Kumar,S., Harle,A., Nanda,J.S. and Raje,M.
  TITLE     The macrophage cell surface glyceraldehyde-3-phosphate
            dehydrogenase is a novel transferrin receptor
  JOURNAL   J Biol Chem 282 (5), 3252-3261 (2007)
   PUBMED   17121833
  REMARK    GeneRIF: GAPDH interacts with transferrin and the GAPDH-transferrin
            complex is subsequently internalized into the early endosomes.
            GeneRIF: Gapdh is a moonlighting protein that functions as a
            glycolytic enzyme and a transferrin receptor.
REFERENCE   8  (bases 1 to 1297)
  AUTHORS   Jones,C.M., Simon-Chazottes,D., Guenet,J.L. and Hogan,B.L.
  TITLE     Isolation of Vgr-2, a novel member of the transforming growth
            factor-beta-related gene family
  JOURNAL   Mol Endocrinol 6 (11), 1961-1968 (1992)
   PUBMED   1480182
REFERENCE   9  (bases 1 to 1297)
  AUTHORS   Welch,J.E., Schatte,E.C., O'Brien,D.A. and Eddy,E.M.
  TITLE     Expression of a glyceraldehyde 3-phosphate dehydrogenase gene
            specific to mouse spermatogenic cells
  JOURNAL   Biol Reprod 46 (5), 869-878 (1992)
   PUBMED   1375514
REFERENCE   10 (bases 1 to 1297)
  AUTHORS   Bruns,G.A. and Gerald,P.S.
  TITLE     Human glyceraldehyde-3-phosphate dehydrogenase in man-rodent
            somatic cell hybrids
  JOURNAL   Science 192 (4234), 54-56 (1976)
   PUBMED   176725
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166162.6.
            
            On Sep 6, 2022 this sequence version replaced NM_001289726.1.
            
            Summary: This gene encodes a member of the
            glyceraldehyde-3-phosphate dehydrogenase protein family. The
            encoded protein has been identified as a moonlighting protein based
            on its ability to perform mechanistically distinct functions. The
            encoded protein was originally identified as a key glycolytic
            enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into
            3-phospho-D-glyceroyl phosphate. Subsequent studies have assigned a
            variety of additional functions to the protein including
            nitrosylation of nuclear proteins, the regulation of mRNA
            stability, and acting as a transferrin receptor on the cell surface
            of macrophage. Alternative splicing results in multiple transcript
            variants. Many pseudogenes similar to this locus are found
            throughout the mouse genome. [provided by RefSeq, Jan 2014].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK144690.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            multifunctional gene product(s) :: PMID: 17121833, 18809573,
                                               20972425
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-94                AC166162.6         139715-139808       c
            95-135              AC166162.6         138592-138632       c
            136-433             AC166162.6         136460-136757       c
            434-631             AC166162.6         136164-136361       c
            632-862             AC166162.6         135799-136029       c
            863-1044            AC166162.6         135533-135714       c
            1045-1297           AC166162.6         135170-135422       c
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 59.32 cM"
     gene            1..1297
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /note="glyceraldehyde-3-phosphate dehydrogenase"
                     /db_xref="GeneID:14433"
                     /db_xref="MGI:MGI:95640"
     exon            1..94
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..1114
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /EC_number="1.2.1.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     peptidyl-cysteine S-nitrosylase GAPDH"
                     /codon_start=1
                     /product="glyceraldehyde-3-phosphate dehydrogenase isoform
                     1"
                     /protein_id="NP_001276655.1"
                     /db_xref="CCDS:CCDS80613.1"
                     /db_xref="GeneID:14433"
                     /db_xref="MGI:MGI:95640"
                     /translation="MLPLPRGPSLGSSGKLRRVFPRPVDKMVKVGVNGFGRIGRLVTR
                     AAICSGKVEIVAINDPFIDLNYMVYMFQYDSTHGKFNGTVKAENGKLVINGKPITIFQ
                     ERDPTNIKWGEAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVN
                     HEKYDNSLKIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGK
                     LWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKP
                     AKYDDIKKVVKQASEGPLKGILGYTEDQVVSCDFNSNSHSSTFDAGAGIALNDNFVKL
                     ISWYDNEYGYSNRVVDLMAYMASKE"
     exon            95..135
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     exon            136..433
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     exon            434..631
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     exon            632..862
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     exon            863..1044
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1297
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1274..1279
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /note="hexamer: AATAAA"
     polyA_site      1297
                     /gene="Gapdh"
                     /gene_synonym="Gapd"
                     /note="major polyA site"
ORIGIN      
        1 ctctgagcct cctccaattc aacccttaag agggatgctg cccttacccc ggggtcccag
       61 cttaggttca tcaggtaaac tcaggagagt gtttcctcgt cccgtagaca aaatggtgaa
      121 ggtcggtgtg aacggatttg gccgtattgg gcgcctggtc accagggctg ccatttgcag
      181 tggcaaagtg gagattgttg ccatcaacga ccccttcatt gacctcaact acatggtcta
      241 catgttccag tatgactcca ctcacggcaa attcaacggc acagtcaagg ccgagaatgg
      301 gaagcttgtc atcaacggga agcccatcac catcttccag gagcgagacc ccactaacat
      361 caaatggggt gaggccggtg ctgagtatgt cgtggagtct actggtgtct tcaccaccat
      421 ggagaaggcc ggggcccact tgaagggtgg agccaaaagg gtcatcatct ccgccccttc
      481 tgccgatgcc cccatgtttg tgatgggtgt gaaccacgag aaatatgaca actcactcaa
      541 gattgtcagc aatgcatcct gcaccaccaa ctgcttagcc cccctggcca aggtcatcca
      601 tgacaacttt ggcattgtgg aagggctcat gaccacagtc catgccatca ctgccaccca
      661 gaagactgtg gatggcccct ctggaaagct gtggcgtgat ggccgtgggg ctgcccagaa
      721 catcatccct gcatccactg gtgctgccaa ggctgtgggc aaggtcatcc cagagctgaa
      781 cgggaagctc actggcatgg ccttccgtgt tcctaccccc aatgtgtccg tcgtggatct
      841 gacgtgccgc ctggagaaac ctgccaagta tgatgacatc aagaaggtgg tgaagcaggc
      901 atctgagggc ccactgaagg gcatcttggg ctacactgag gaccaggttg tctcctgcga
      961 cttcaacagc aactcccact cttccacctt cgatgccggg gctggcattg ctctcaatga
     1021 caactttgtc aagctcattt cctggtatga caatgaatac ggctacagca acagggtggt
     1081 ggacctcatg gcctacatgg cctccaagga gtaagaaacc ctggaccacc caccccagca
     1141 aggacactga gcaagagagg ccctatccca actcggcccc caacactgag catctccctc
     1201 acaatttcca tcccagaccc ccataataac aggaggggcc tagggagccc tccctactct
     1261 cttgaatacc atcaataaag ttcgctgcac ccacctc
//
