LOCUS       NM_001291002            2523 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus galactokinase 2 (Galk2), transcript variant 1, mRNA.
ACCESSION   NM_001291002 XM_006500152
VERSION     NM_001291002.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Bulusu,V., Prior,N., Snaebjornsson,M.T., Kuehne,A., Sonnen,K.F.,
            Kress,J., Stein,F., Schultz,C., Sauer,U. and Aulehla,A.
  TITLE     Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to
            Mouse Embryo Mesoderm Development
  JOURNAL   Dev Cell 40 (4), 331-341 (2017)
   PUBMED   28245920
REFERENCE   3  (bases 1 to 2523)
  AUTHORS   Phillips,R.L., Ernst,R.E., Brunk,B., Ivanova,N., Mahan,M.A.,
            Deanehan,J.K., Moore,K.A., Overton,G.C. and Lemischka,I.R.
  TITLE     The genetic program of hematopoietic stem cells
  JOURNAL   Science 288 (5471), 1635-1640 (2000)
   PUBMED   10834841
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BU698705.1, BC079843.1 and AK082521.1.
            
            On Mar 21, 2014 this sequence version replaced XM_006500152.1.
            
            Transcript Variant: This variant (1) represents the shorter
            transcript but encodes the longer isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC079843.1, AK154762.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                BU698705.1         1-30
            31-1812             BC079843.1         1-1782
            1813-2523           AK082521.1         1810-2520
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 61.76 cM"
     gene            1..2523
                     /gene="Galk2"
                     /gene_synonym="2810017M24Rik; Gk2"
                     /note="galactokinase 2"
                     /db_xref="GeneID:69976"
                     /db_xref="MGI:MGI:1917226"
     exon            1..163
                     /gene="Galk2"
                     /gene_synonym="2810017M24Rik; Gk2"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..1487
                     /gene="Galk2"
                     /gene_synonym="2810017M24Rik; Gk2"
                     /EC_number="2.7.1.157"
                     /note="isoform a is encoded by transcript variant 1;
                     N-acetylgalactosamine kinase; galNAc kinase"
                     /codon_start=1
                     /product="N-acetylgalactosamine kinase isoform a"
                     /protein_id="NP_001277931.1"
                     /db_xref="CCDS:CCDS71134.1"
                     /db_xref="GeneID:69976"
                     /db_xref="MGI:MGI:1917226"
                     /translation="MAAEDPATRRVQVAEHPRLLKLKEMFNSKFGSTPKFYVRAPGRV
                     NIIGEHIDYCGYSVIPMAVEQDMLIAVEPVKTHTLQLANTDPLYPDFSTTANNICIDK
                     TKPLWHNYFLCGFKGIQEHFGLSKLPGMNCLVDGNIPPSSGLSSSSALVCCAGLVTLT
                     VLGLRLSKVELAEICAKSERYIGTEGGGMDQSISFLAEEGTAKLIEFSPLRATNVKLP
                     SGAVFVIANSCMEMNKAATSHFNVRVMECRLAAKVLAKHKGLQWDNVLRLEEVQSKLG
                     ISLEEMLLVTEDALHPEPYSREEICRCLGISLERLRTQILTPNTQDELTFKLYQRAKH
                     VYSEAARVLQFKQVCEDAPDNAVQLLGELMNQSHRSCRDMYECSCPELDQLVDICRKF
                     GAKGSRLTGAGWGGCTVSLVPADMLSSFLASVHEAYYQGNTSRLAQEKHSLFATKPGG
                     GALVFREV"
     exon            164..252
                     /gene="Galk2"
                     /gene_synonym="2810017M24Rik; Gk2"
                     /inference="alignment:Splign:2.1.0"
     exon            253..376
                     /gene="Galk2"
                     /gene_synonym="2810017M24Rik; Gk2"
                     /inference="alignment:Splign:2.1.0"
     exon            377..467
                     /gene="Galk2"
                     /gene_synonym="2810017M24Rik; Gk2"
                     /inference="alignment:Splign:2.1.0"
     exon            468..614
                     /gene="Galk2"
                     /gene_synonym="2810017M24Rik; Gk2"
                     /inference="alignment:Splign:2.1.0"
     exon            615..713
                     /gene="Galk2"
                     /gene_synonym="2810017M24Rik; Gk2"
                     /inference="alignment:Splign:2.1.0"
     exon            714..866
                     /gene="Galk2"
                     /gene_synonym="2810017M24Rik; Gk2"
                     /inference="alignment:Splign:2.1.0"
     exon            867..1077
                     /gene="Galk2"
                     /gene_synonym="2810017M24Rik; Gk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..1279
                     /gene="Galk2"
                     /gene_synonym="2810017M24Rik; Gk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..2523
                     /gene="Galk2"
                     /gene_synonym="2810017M24Rik; Gk2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1793..1798
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Galk2"
                     /gene_synonym="2810017M24Rik; Gk2"
                     /note="hexamer: ATTAAA"
     polyA_site      1812
                     /gene="Galk2"
                     /gene_synonym="2810017M24Rik; Gk2"
                     /note="major polyA site"
ORIGIN      
        1 acctgcagga tttccgctga gatcccggaa gaaagccatt cctgccactt taatctcgtg
       61 atgcttagct gtgctgcact tctggaaact gcagagggat ctagcaaaat atggctgcgg
      121 aggaccctgc cactcgacga gtccaggtgg cagaacatcc cagattgctg aagctaaaag
      181 agatgtttaa ctctaaattt gggtccacac ccaagtttta tgttcgagcc ccaggaagag
      241 tcaacataat aggtgagcac atagattact gtggatattc cgttattccc atggctgtgg
      301 aacaagacat gttaatagct gtggaacctg tgaaaacaca cactctccaa ctggctaata
      361 cagacccctt gtatccagac ttcagtacta cagctaataa catctgtatc gacaagacca
      421 aacctctatg gcacaactac ttcttgtgtg gctttaaggg aattcaggaa cactttggcc
      481 ttagtaagct gcctggaatg aattgcctgg tagatggaaa tatacccccg agttcgggcc
      541 tctccagctc cagtgctctg gtttgctgtg ctgggttagt gactctcacg gtgctgggac
      601 tgagactttc caaggtggaa cttgcagaaa tctgtgccaa gagtgagcgc tacataggca
      661 ctgaaggtgg cggcatggac cagtccatat cattccttgc agaagaagga actgccaagt
      721 taatagaatt tagccctctg agagcgacta atgtgaagct ccccagtggg gcagtctttg
      781 tgattgccaa cagctgcatg gaaatgaaca aggcggccac ttcacatttc aatgtcaggg
      841 tgatggagtg tcgactggcg gcaaaggtcc ttgctaaaca caaaggcttg caatgggata
      901 atgtcctgcg gctagaggaa gtgcaaagca aactcgggat tagtctggaa gagatgctgt
      961 tggtcacaga ggatgccctt catcctgaac cctacagccg ggaggagatc tgcaggtgcc
     1021 tgggaattag cctcgagaga ctccgcaccc aaatcctgac tccaaatact caagatgaac
     1081 tcacctttaa gctctaccag cgggccaagc acgtgtacag tgaggctgca cgcgtgctgc
     1141 agtttaagca ggtatgtgaa gatgcgccag acaacgcagt ccagctgctg ggggagctaa
     1201 tgaaccagag ccacagaagc tgccgggaca tgtatgagtg cagctgccct gagctggacc
     1261 agctagtgga catctgccgg aagtttgggg ccaaaggctc acgacttact ggagcaggat
     1321 ggggaggctg cactgtgtcc ctcgtgcccg ctgacatgct gtccagcttt ctcgccagtg
     1381 tgcacgaagc ttattaccag gggaacacat cccgcctggc tcaggagaag catagcttgt
     1441 ttgccaccaa gcctggaggc ggggccttgg ttttccgtga ggtctaaaca atgtcaaaca
     1501 tctcagagaa actattttgg gtattaagga gctggcgggg cttcctgtgc cacagtaaat
     1561 cagctctttt tctgttttgt gttgtgaggt acgtttgcta ttatcaagat gcatttctaa
     1621 agaaatggtg aaagctagac ttcataatga ccacttttct ttcttacatg tatttttatg
     1681 aatcagagac aaggatatgc ttagatagac ctttgaggta tgattaagat ttattgatat
     1741 gaagatttaa aagatgaaca gcaaaatgta cttttaattg aacactgctc caattaaact
     1801 gatacaactg aactgtgtgt tgctcttgaa tggcggccac tgctggcttc ccttagccct
     1861 cttccacatt gttgaagtct tctgatatag taggaaatga cgatgatgat tgtggtgact
     1921 gcatcgactg tggcgactct gatgctgatg ttgatcctcc atattcctcc ttgagttttg
     1981 cctcaacacg ctaggaagaa agtgcatggt taagggtatg cagacgttag acacacacag
     2041 tacacttgaa tgtcagattt ctacatctct gaagatggtg aagatcactt tgggaagagg
     2101 agccaaaaga tagatttcag aagacaattt gaattgactg tcctttcact agcacttcta
     2161 cttgtccaat gcctttccat cctgacagcc ccatccatac ctcacatccc tcttgtggct
     2221 gcctggcaag gcttgtttgt atacctggtt ccctgtaggg cagatgtgca gtacacagct
     2281 ttgggtgaat aagcaaaagc ctaaagtgtg aaccgacagt gatgaagtaa gggcacaagg
     2341 ctctggtcac gtggtgacag gccaatggca gacatccgga agccctttct aagtttcatg
     2401 gcagatgaaa gagggtttgg ctaaaacaat caggtgacag aggtagcttg tattgtgtta
     2461 gaaatcacca tggttgttgt ttcttatgat tgtgttggcc cattaaaagg catttagagg
     2521 ctg
//
