LOCUS       NM_016922               1969 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus galactose-3-O-sulfotransferase 1 (Gal3st1), transcript
            variant 1, mRNA.
ACCESSION   NM_016922
VERSION     NM_016922.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Dustin,E., McQuiston,A.R., Honke,K., Palavicini,J.P., Han,X. and
            Dupree,J.L.
  TITLE     Adult-onset depletion of sulfatide leads to axonal degeneration
            with relative myelin sparing
  JOURNAL   Glia 71 (9), 2285-2303 (2023)
   PUBMED   37283058
REFERENCE   3  (bases 1 to 1969)
  AUTHORS   Qiu,S., Palavicini,J.P., Wang,J., Gonzalez,N.S., He,S., Dustin,E.,
            Zou,C., Ding,L., Bhattacharjee,A., Van Skike,C.E., Galvan,V.,
            Dupree,J.L. and Han,X.
  TITLE     Adult-onset CNS myelin sulfatide deficiency is sufficient to cause
            Alzheimer's disease-like neuroinflammation and cognitive impairment
  JOURNAL   Mol Neurodegener 16 (1), 64 (2021)
   PUBMED   34526055
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1969)
  AUTHORS   McGonigal,R., Barrie,J.A., Yao,D., McLaughlin,M., Cunningham,M.E.,
            Rowan,E.G. and Willison,H.J.
  TITLE     Glial Sulfatides and Neuronal Complex Gangliosides Are Functionally
            Interdependent in Maintaining Myelinating Axon Integrity
  JOURNAL   J Neurosci 39 (1), 63-77 (2019)
   PUBMED   30446529
REFERENCE   5  (bases 1 to 1969)
  AUTHORS   Kunisawa,K., Shimizu,T., Kushima,I., Aleksic,B., Mori,D.,
            Osanai,Y., Kobayashi,K., Taylor,A.M., Bhat,M.A., Hayashi,A.,
            Baba,H., Ozaki,N. and Ikenaka,K.
  TITLE     Dysregulation of schizophrenia-related aquaporin 3 through
            disruption of paranode influences neuronal viability
  JOURNAL   J Neurochem 147 (3), 395-408 (2018)
   PUBMED   30025158
REFERENCE   6  (bases 1 to 1969)
  AUTHORS   Ishibashi,T., Ding,L., Ikenaka,K., Inoue,Y., Miyado,K., Mekada,E.
            and Baba,H.
  TITLE     Tetraspanin protein CD9 is a novel paranodal component regulating
            paranodal junctional formation
  JOURNAL   J Neurosci 24 (1), 96-102 (2004)
   PUBMED   14715942
REFERENCE   7  (bases 1 to 1969)
  AUTHORS   Ishibashi,T., Dupree,J.L., Ikenaka,K., Hirahara,Y., Honke,K.,
            Peles,E., Popko,B., Suzuki,K., Nishino,H. and Baba,H.
  TITLE     A myelin galactolipid, sulfatide, is essential for maintenance of
            ion channels on myelinated axon but not essential for initial
            cluster formation
  JOURNAL   J Neurosci 22 (15), 6507-6514 (2002)
   PUBMED   12151530
REFERENCE   8  (bases 1 to 1969)
  AUTHORS   Honke,K., Hirahara,Y., Dupree,J., Suzuki,K., Popko,B.,
            Fukushima,K., Fukushima,J., Nagasawa,T., Yoshida,N., Wada,Y. and
            Taniguchi,N.
  TITLE     Paranodal junction formation and spermatogenesis require
            sulfoglycolipids
  JOURNAL   Proc Natl Acad Sci U S A 99 (7), 4227-4232 (2002)
   PUBMED   11917099
REFERENCE   9  (bases 1 to 1969)
  AUTHORS   Hirahara,Y., Tsuda,M., Wada,Y. and Honke,K.
  TITLE     cDNA cloning, genomic cloning, and tissue-specific regulation of
            mouse cerebroside sulfotransferase
  JOURNAL   Eur J Biochem 267 (7), 1909-1917 (2000)
   PUBMED   10727929
REFERENCE   10 (bases 1 to 1969)
  AUTHORS   Honke,K., Tsuda,M., Hirahara,Y., Ishii,A., Makita,A. and Wada,Y.
  TITLE     Molecular cloning and expression of cDNA encoding human
            3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase
  JOURNAL   J Biol Chem 272 (8), 4864-4868 (1997)
   PUBMED   9030544
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK007645.1, AL807395.8, CX239940.1 and BB804195.1.
            
            On Apr 25, 2010 this sequence version replaced NM_016922.2.
            
            Transcript Variant: This variant (1, also known as form A)
            represents the longest transcript. Variants 1, 2 and 3 encode the
            same protein. An in-frame AUG is located 29 codons upstream of the
            annotated translation start site but is not being annotated as a
            start site since it would be inhibited by an overlapping uORF with
            a strong Kozak sequence.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC026806.1, SRR7652917.576936.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream ORF predicted to
                                                inhibit translation from
                                                upstream AUG
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-205               AK007645.1         1-205
            206-423             AL807395.8         55950-56167
            424-487             AK007645.1         206-269
            488-488             CX239940.1         182-182
            489-1222            AK007645.1         271-1004
            1223-1676           AL807395.8         69591-70044
            1677-1969           BB804195.1         73-365
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 2.81 cM"
     gene            1..1969
                     /gene="Gal3st1"
                     /gene_synonym="Cst; Gcst"
                     /note="galactose-3-O-sulfotransferase 1"
                     /db_xref="GeneID:53897"
                     /db_xref="MGI:MGI:1858277"
     exon            1..205
                     /gene="Gal3st1"
                     /gene_synonym="Cst; Gcst"
                     /inference="alignment:Splign:2.1.0"
     exon            206..423
                     /gene="Gal3st1"
                     /gene_synonym="Cst; Gcst"
                     /inference="alignment:Splign:2.1.0"
     exon            424..566
                     /gene="Gal3st1"
                     /gene_synonym="Cst; Gcst"
                     /inference="alignment:Splign:2.1.0"
     CDS             436..1707
                     /gene="Gal3st1"
                     /gene_synonym="Cst; Gcst"
                     /EC_number="2.8.2.11"
                     /note="3'-phosphoadenosine-5'-phosphosulfate:Galcer
                     sulfotransferase; cerebroside sulfotransferase;
                     3'-phosphoadenylylsulfate-galactosylceramide
                     3'-sulfotransferase"
                     /codon_start=1
                     /product="galactosylceramide sulfotransferase"
                     /protein_id="NP_058618.2"
                     /db_xref="CCDS:CCDS24373.1"
                     /db_xref="GeneID:53897"
                     /db_xref="MGI:MGI:1858277"
                     /translation="MTLLPKKPCKSKAKGLLLGALFTSFLLLLYSYVVPPLYPNMAFT
                     TSEAAAPCSPIPNEPVAATPANGSAGGCQPRRDIVFMKTHKTASSTLLNILFRFGQKH
                     ELKFAFPNGRNDFHYPSYFARSLVQDYRPGACFNIICNHMRFHYEEVRGLVRPGATFI
                     TVIRDPARLFESSFHYFGSVVPLTWKLSSRDKLAEFLQDPDRYYDPSSYNAHYLRNLL
                     FFDLGYDSSLDPASPRVQEHILEVERRFHLVLLQEYFDESLVLLRELLCWDLEDVLYF
                     KLNARRDSPVPRLSGELYRRATAWNLLDVRLYRHFNASFWRKVEAFGRERMAREVAEL
                     RQANEHMRHICIDGGQAVGAEAIQDSAMQPWQPLGIKSILGYNLKKSIGPQHEQLCRR
                     MLTPEIQYLSDLGANLWVTKLWKFLRDFLRW"
     exon            567..1969
                     /gene="Gal3st1"
                     /gene_synonym="Cst; Gcst"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1947..1952
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gal3st1"
                     /gene_synonym="Cst; Gcst"
                     /note="hexamer: AGTAAA"
     polyA_site      1956
                     /gene="Gal3st1"
                     /gene_synonym="Cst; Gcst"
                     /note="major polyA site"
ORIGIN      
        1 gggccgggcc aggctgctgg cctcacctgg cgagtgttga gagcggggtg cggcagccgc
       61 tgcggcggcg gggcgggggc atggaccagg cctgccagcg cgccgggtcc cggcgagtgc
      121 gccccgccgc ccagtgagcg ccacggcggc ggcgggggcc ggggccgacc cctgccaccc
      181 ggagcccaag gagaacaggg caaagtgcct ctcactgggc tccaaggaag aacaaacaag
      241 cacttgcagg aaagcagtga ctcacaggac aatggcaatg ggggactcta ggccccctca
      301 gtggcttggg gcaagcttct tgctttggtt tgactccatg aaatgtgaat gactagagaa
      361 gacacagcgg gatggagcag aagtacccca aaggttacca gactggagac ctggagtcat
      421 caggggtttc ctgagatgac tctgctgcca aagaagccct gcaagtccaa ggccaagggg
      481 ttactgctgg gagctctctt caccagtttc ctattgctgc tgtactccta tgtggtacct
      541 ccactgtatc ccaacatggc cttcacgacc tcagaggctg cagcaccctg ctcccctatt
      601 cccaatgagc cagtggcagc cactcccgcc aacggctcag caggagggtg tcaacctcgg
      661 cgagatattg tgttcatgaa gacgcacaag accgccagca gcacactgct caacatcctg
      721 ttccgcttcg gccagaagca cgagctcaag ttcgctttcc ccaatggccg aaacgacttc
      781 cactacccgt cgtactttgc acgaagcctg gtgcaggact accggcctgg ggcgtgcttc
      841 aacatcatct gcaaccacat gcgcttccac tatgaggaag tgcgcgggct ggtgcggccg
      901 ggtgccacct tcatcaccgt catccgcgac cctgctcgtc tcttcgagtc ttccttccac
      961 tactttggat ccgtggtgcc gctcacctgg aagctgtcga gccgcgacaa gctggccgag
     1021 ttcctgcagg accccgatcg ctactacgac ccgagcagct acaacgcgca ctacctccgc
     1081 aacctgctct tcttcgatct gggctatgat agtagcctgg acccggccag cccgcgcgtg
     1141 caggagcaca tcctggaggt ggagcgccgc ttccacttgg tgctactgca ggagtacttc
     1201 gacgaatccc tggtgctgct ccgagagctg ctgtgctggg acctggagga cgtgctgtat
     1261 ttcaagctca atgcgcggcg cgactcgccg gttccgcggc tctctggcga gctgtaccgc
     1321 cgagccaccg cctggaacct gctggacgtg cgcctctacc gtcacttcaa cgccagcttc
     1381 tggcgaaagg tggaagcttt cgggcgtgag cgcatggcgc gcgaggtagc cgagctgcgc
     1441 caagcgaacg agcacatgcg ccacatctgc atagatggcg gccaagcagt tggtgctgag
     1501 gccatccagg actcggccat gcagccctgg caacccctgg gcatcaagtc catcctgggt
     1561 tacaacctca agaagagcat cgggccacaa cacgagcagc tctgccggcg catgctcacg
     1621 cccgaaatcc agtacctgtc tgacctcggt gccaatctct gggtcaccaa gctctggaag
     1681 ttccttaggg actttctaag gtggtgatgt atcctgccca cctcctgttt ggcttcctga
     1741 tgcagcaagg ccgggcaggg agcctctggc acctagcctt ccctagccac acctagtgcc
     1801 accctggggt ccctagggtc atagctgtct gggactagtc tctctgcagg agagagccta
     1861 acggagaggt atttaactaa ttatgccatt ttttttttat taaatcccct ttatttccag
     1921 cctcctctta caaggggaga cgcagaagta aagaaatttt atgtgtgtg
//
