LOCUS       NM_153569               4798 bp    mRNA    linear   ROD 16-JUN-2024
DEFINITION  Mus musculus cyclin G associated kinase (Gak), transcript variant
            1, mRNA.
ACCESSION   NM_153569 XM_132229
VERSION     NM_153569.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Tian,X., Inoue,K., Zhang,Y., Wang,Y., Sperati,C.J., Pedigo,C.E.,
            Zhao,T., Yan,M., Groener,M., Moledina,D.G., Ebenezer,K., Li,W.,
            Zhang,Z., Liebermann,D.A., Greene,L., Greer,P., Parikh,C.R. and
            Ishibe,S.
  TITLE     Inhibiting calpain 1 and 2 in cyclin G associated kinase-knockout
            mice mitigates podocyte injury
  JOURNAL   JCI Insight 5 (22), 142740 (2020)
   PUBMED   33208557
  REMARK    GeneRIF: Inhibiting calpain 1 and 2 in cyclin G associated
            kinase-knockout mice mitigates podocyte injury.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Lin,A.W., Gill,K.K., Castaneda,M.S., Matucci,I., Eder,N.,
            Claxton,S., Flynn,H., Snijders,A.P., George,R. and Ultanir,S.K.
  TITLE     Chemical genetic identification of GAK substrates reveals its role
            in regulating Na+/K+-ATPase
  JOURNAL   Life Sci Alliance 1 (6), e201800118 (2018)
   PUBMED   30623173
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4798)
  AUTHORS   Park,B.C., Yim,Y.I., Zhao,X., Olszewski,M.B., Eisenberg,E. and
            Greene,L.E.
  TITLE     The clathrin-binding and J-domains of GAK support the uncoating and
            chaperoning of clathrin by Hsc70 in the brain
  JOURNAL   J Cell Sci 128 (20), 3811-3821 (2015)
   PUBMED   26345367
  REMARK    GeneRIF: PTEN-like domains of GAK and auxilin are not essential for
            Hsc70-dependent chaperoning and uncoating of clathrin, but
            depending on the tissue, these domains appear to increase the
            efficiency of these co-chaperones.
REFERENCE   4  (bases 1 to 4798)
  AUTHORS   Beilina,A., Rudenko,I.N., Kaganovich,A., Civiero,L., Chau,H.,
            Kalia,S.K., Kalia,L.V., Lobbestael,E., Chia,R., Ndukwe,K., Ding,J.,
            Nalls,M.A., Olszewski,M., Hauser,D.N., Kumaran,R., Lozano,A.M.,
            Baekelandt,V., Greene,L.E., Taymans,J.M., Greggio,E. and
            Cookson,M.R.
  CONSRTM   International Parkinson's Disease Genomics Consortium; North
            American Brain Expression Consortium
  TITLE     Unbiased screen for interactors of leucine-rich repeat kinase 2
            supports a common pathway for sporadic and familial Parkinson
            disease
  JOURNAL   Proc Natl Acad Sci U S A 111 (7), 2626-2631 (2014)
   PUBMED   24510904
REFERENCE   5  (bases 1 to 4798)
  AUTHORS   Olszewski,M.B., Chandris,P., Park,B.C., Eisenberg,E. and
            Greene,L.E.
  TITLE     Disruption of clathrin-mediated trafficking causes centrosome
            overduplication and senescence
  JOURNAL   Traffic 15 (1), 60-77 (2014)
   PUBMED   24138026
  REMARK    GeneRIF: GAK knockout causes senescence and centrosome duplication
            in MEF cells.
REFERENCE   6  (bases 1 to 4798)
  AUTHORS   Lee,D.W., Zhao,X., Yim,Y.I., Eisenberg,E. and Greene,L.E.
  TITLE     Essential role of cyclin-G-associated kinase (Auxilin-2) in
            developing and mature mice
  JOURNAL   Mol Biol Cell 19 (7), 2766-2776 (2008)
   PUBMED   18434600
  REMARK    GeneRIF: GAK deletion blocks development and causes lethality in
            adult animals by disrupting clathrin-mediated endocytosis
REFERENCE   7  (bases 1 to 4798)
  AUTHORS   Lin,Y., Tang,Y.J., Zong,H.L., Gu,J.X., Deng,W.W., Wang,C. and
            Sun,B.
  TITLE     Cyclin G associated kinase interacts with interleukin 12 receptor
            beta2 and suppresses interleukin 12 induced IFN-gamma production
  JOURNAL   FEBS Lett 581 (26), 5151-5157 (2007)
   PUBMED   17937931
REFERENCE   8  (bases 1 to 4798)
  AUTHORS   Yin,X., Warner,D.R., Roberts,E.A., Pisano,M.M. and Greene,R.M.
  TITLE     Identification of novel CBP interacting proteins in embryonic
            orofacial tissue
  JOURNAL   Biochem Biophys Res Commun 329 (3), 1010-1017 (2005)
   PUBMED   15752756
REFERENCE   9  (bases 1 to 4798)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   10 (bases 1 to 4798)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY312464.1, AK166682.1, AI466057.1 and AC133896.7.
            
            On Aug 15, 2013 this sequence version replaced NM_153569.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC065052.1, AK146695.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                BY312464.1         2-23
            23-4484             AK166682.1         2-4463
            4485-4696           AI466057.1         50-261
            4697-4697           AC133896.7         138001-138001       c
            4698-4798           AI466057.1         263-363
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 53.2 cM"
     gene            1..4798
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /note="cyclin G associated kinase"
                     /db_xref="GeneID:231580"
                     /db_xref="MGI:MGI:2442153"
     exon            1..277
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            278..339
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            340..399
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            400..514
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            515..657
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            658..783
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            784..873
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            874..1009
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1122
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1207
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1331
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1381
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1530
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1531..1653
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1654..1787
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1788..1982
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2100
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2101..2180
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2181..2374
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2375..2521
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2522..3001
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3002..3172
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3173..3295
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3296..3412
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3413..3625
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3626..3771
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3772..3948
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3949..4798
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4464..4469
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      4491
                     /gene="Gak"
                     /gene_synonym="D130045N16Rik"
                     /note="major polyA site"
ORIGIN      
        1 gtcgggacgc aactcgctct ctgatttacg tcacggcgcg ggcggaagat ggccgggttg
       61 ttcgcccgca gctgctaacc cggcagtgcg gagctggagc ctggctcccg ctgccgcgga
      121 gcggccgacg ccatgtcgct gctgcagtct gcgctggact tcctggcggg ccctggttct
      181 cttggcggag ctgcgggccg tgatcagagt gacttcgtgg ggcagacagt ggagctgggc
      241 gagctgcgtc tgcgggtgcg gcgggtcctg gccgagggag ggtttgcttt tgtttatgaa
      301 gctcaagatc tgggaagtgg cagagagtat gcattaaagc gattactatc caatgaagag
      361 gaaaagaaca gagccatcat tcaggaagtt tgtttcttga aaaaactttc tggccacccc
      421 aatattgtcc agttctgctc tgcagcatcc ataggaaaag aggagtcgga cactgggcag
      481 gctgagttcc tcctgcttac ggagctttgt aaaggacagc tggtggagtt tctcaagaga
      541 gttgaatgta aaggtcctct gtcctgcgac agcattctga agatcttcta ccagacatgc
      601 agagcagtgc agcacatgca caggcagaaa ccacccatca tccacagaga tctcaaggtt
      661 gaaaacttac tgcttagtaa ccaggggacc attaagctgt gtgactttgg cagtgccaca
      721 accatctccc attatcctga ctacagctgg agtgcccaga agcgagccat ggtggaggaa
      781 gagatcacaa ggaacaccac acccatgtac agaacaccag aaattgtaga cctgtattcc
      841 aacttcccca ttggcgaaaa gcaggatatc tgggcactgg gctgtatctt atacctgctg
      901 tgtttccggc agcatccttt tgaggatgga gcaaaacttc ggatagtcaa tgggaagtat
      961 tccattcctg tgaatgacac tcgttacaca gtcttccatg accttattcg tgccatgcta
     1021 aaggtcaacc cagaggagag gctgtccatt gctgaagtgg tccgacaact gcaggaaatt
     1081 gcagcagcta ggaatgtgaa ccccaaagct cccatcacag agctgctgga gcagaatggt
     1141 ggctatggga actcagggcc ttcccgagca caaccacctt gcgggggcac cgtgaacagc
     1201 agtggagttt tggctctggc agagtatgac cagccctacg gtgggtttct tgatattctg
     1261 cggggtggga ccgaacggct cttcaccaac ctcaaggata cttcctccaa ggtcatccag
     1321 tctgtggcta actatgcaaa gggtgatctg gacatatctt acatcacatc caggattgca
     1381 gtgatgtcat tcccagcaga aggtgtggag tctgcaatca aaaacaatat agaggatgtg
     1441 cgaatgtttc tggatgctaa gcatccagga cattatgctg tctacaacct ttctccaagg
     1501 atataccggg cttccaagtt tcacaaccgg gtcactgagt gtggctgggc agtcagacgg
     1561 gcaccacatc tccacagttt gtatactcta tgtaggagca tgcatgcctg gctccgggaa
     1621 gaccacagga acgtctgtgt tgtgcattgc atggatggga gagctgcatc tgctgtggca
     1681 gtctgtgcat tcctgtgctt ctgccgtctc ttcagcactg cagaggctgc tgtgtacatg
     1741 ttcagcatga agcgctgccc accaggcatt tggccatccc acaaaaggta cattgaatat
     1801 gtatgtgaca tggtggcaga ggagcccatc acgccccata gcaagccaat gctggtgaaa
     1861 tctgttgtca tgacccccgt gccactgttc agcaagcaga ggaatggctg ccgaccgttc
     1921 tgtgaggtct acgttggaga ggagcgtgtc accaccacgt cgcaggaata tgacagaatg
     1981 aaggaattta aaattgagga tggcaaggct gtcatccccc tgggcgtaac agttcaagga
     2041 gacgtgctca tcatcatcta ccatgccagg gccacactgg gagggaggct gcaggctaag
     2101 atggcgtcca tgaaaatgtt ccagatccag ttccacactg ggttcgtgcc tcgaaacgca
     2161 accactgtga aatttgcaaa atatgacctg gatgcttgtg atattcaaga gaagtaccca
     2221 gatctgttcc aggtgaacct ggaagtggag gtagagccta gagacaggcc cagccgagaa
     2281 gctccacctt gggagaacac cagcctaagg gggttaaacc ccaagatcct cttttccaac
     2341 agggaagagc agcaggacat tctgtctaag tttgggaagc cggagctacc ccggcagccg
     2401 ggctccacag ctcagtatga tgctgaggca gggtctccgg aggctgagat cacagagtcg
     2461 gactcaccgc agagcagcag tacggacacc aaccactttc ttcacacact ggattggcag
     2521 gaggaaaaag aaccagagac tgggttagac aatacctctc ctaaggagag tcagtctgtc
     2581 ctgattgcag acggagatgg aagtgaagta tcagatgaag aagaggcttc attccccagt
     2641 gaggagagga aaccaggagc tggagaagat acaccaaggc tggctgctgg gaccaaacag
     2701 caagacttaa tatttgatgt gggcatgctg gctgccccac aggagcctgt acagcccgaa
     2761 gaaggtgttg atctcctggg gctacactct gagggggact taaggcctgc tgccccctta
     2821 caggcttgtg gggtcccatc tagcaacact gacctgttga gctgccttct tgaaccatct
     2881 gatgctgctc aagtagggcc tcctggtgac ctgcttggtg gtgaggctcc actgctgcta
     2941 gcaagcccag tttctcctct tgggctgcag aacaacctac aaggaaaagt ccctgacact
     3001 gtggacccat ttgaccagtt cctgctgtca tccaactcag acacccagcc ctgctccaag
     3061 cctgatctct ttggagagtt tctcaactct gactctgtag cttcctcaac tgccttccca
     3121 tccacccaca gtgccccacc cccatcctgt agcactgcct tcctgcacct aggggatctg
     3181 ccagcagagc ccagcaaggt aatagcttca tccagccacc cagatctgct aggaggatgg
     3241 gatacgtggg ctgacacagc tacacctggg ccagcctcca ttccagtacc agaaggtacc
     3301 ctcttctctt ctgcaggtca cccagcccct cctggcccca accccagcca aaccaagtct
     3361 cagaaccttg acccatttgc tgatctcagt gatctcagct ctagcctcca aggcctgcct
     3421 gctggacttc ctgcaggggg ctttgttggc gcaccagccc ccactcaaaa gagcaacagc
     3481 ccctggcagg caaatcgtcc cacagcccct ggaacctcat ggacccccca ggccaagcca
     3541 gcccccagag cctctgaaca gctaaggtct cacttcagtg tgatcggggc ccgagaagag
     3601 aggggcgtcc gtgtgcccag ctttgcccaa aagccaaagg tctcagaaaa tgattttgaa
     3661 gatcttctgc ctaatcaagg cttctctaag tctgacaaga aggggccaaa gaccatggca
     3721 gagatgcgga aacaggaact tgccagagat acagacccac tcaaattgaa gcttttggac
     3781 tggattgaag gcaaggagag gaacattcgt gcactgctgt ccactctgca cacggtattg
     3841 tgggatgggg agagccgctg gacacctgtg agtatggctg acctggtgac tccagagcag
     3901 gtgaagaagc agtaccgccg tgcagtgctg gtagtgcatc ctgataaggc cacagggcag
     3961 ccgtatgaac agtatgccaa gatgatcttc atggagctga atgatgcatg gtctgagttt
     4021 gaaaaccagg gctcgaggcc cctcttctga ggcctgtgat ggctatggct gcaggagctg
     4081 ttcatgaatc ctgaatgctg ctggcctgac tgaggcacaa atgggaccat aacaggtgtg
     4141 gcaggtgata tcgccccagg ggcctggacc atctactccc aatcatccct gcattcatca
     4201 tggacaaata ccattccatg gctgggagag aaagcattcc tcagatctga tgttagttgt
     4261 tttgtttttc tttttcttgt accagaggaa aagtgttgat ttatattcct tccacagctg
     4321 tcacagtttg tgatttactt ggtgtctggt ggctgtcacc tcttggatgg cacatgtcgc
     4381 tggcacttct ctcctgtcat ttcttcaaat atgggggtac atccacatcc tactctgatc
     4441 agtttgccaa tcatctgtac ataattaaac tattttctga tggctggtgc agtctctggc
     4501 ttgtaaagca cttcttacct agtacctggt cacctatttg ttcctcggtt tttttaccca
     4561 tgatggattg taggattgtg aagttggtct aggattggct gtaaagacca ctgcagccag
     4621 caccaatacc atcttcactg agcattttgc taaagaaaag ttctagaaac acatcagaag
     4681 ggagcctgat ccacaacatt gggtttctcc caaggcctag agtcttaaac tattttcaga
     4741 aattctactg tacttgatct tagcgcataa ctagagttct tagaggggtg tcaggaca
//
