LOCUS       NM_008077               3283 bp    mRNA    linear   ROD 18-MAR-2023
DEFINITION  Mus musculus glutamate decarboxylase 1 (Gad1), transcript variant
            1, mRNA.
ACCESSION   NM_008077
VERSION     NM_008077.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ronstrom JW, Johnson NL, Jones ST, Werner SJ, Wadsworth HA,
            Brundage JN, Stolp V, Graziane NM, Silberman Y, Steffensen SC and
            Yorgason JT.
  TITLE     Opioid-Induced Reductions in Amygdala Lateral Paracapsular GABA
            Neuron Circuit Activity
  JOURNAL   Int J Mol Sci 24 (3), 1929 (2023)
   PUBMED   36768252
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Ruiz-Reig N, Garcia-Sanchez D, Schakman O, Gailly P and Tissir F.
  TITLE     Inhibitory synapse dysfunction and epileptic susceptibility
            associated with KIF2A deletion in cortical interneurons
  JOURNAL   Front Mol Neurosci 15, 1110986 (2023)
   PUBMED   36733270
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3283)
  AUTHORS   Liaci C, Camera M, Zamboni V, Saro G, Ammoni A, Parmigiani E,
            Ponzoni L, Hidisoglu E, Chiantia G, Marcantoni A, Giustetto M,
            Tomagra G, Carabelli V, Torelli F, Sala M, Yanagawa Y, Obata K,
            Hirsch E and Merlo GR.
  TITLE     Loss of ARHGAP15 affects the directional control of migrating
            interneurons in the embryonic cortex and increases susceptibility
            to epilepsy
  JOURNAL   Front Cell Dev Biol 10, 875468 (2022)
   PUBMED   36568982
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3283)
  AUTHORS   Ye Q, Nunez J and Zhang X.
  TITLE     Raphe serotonin projections dynamically regulate feeding behavior
            through targeting inhibitory circuits from rostral zona incerta to
            paraventricular thalamus
  JOURNAL   Mol Metab 66, 101634 (2022)
   PUBMED   36351530
REFERENCE   5  (bases 1 to 3283)
  AUTHORS   Magno L, Asgarian Z, Apanaviciute M, Milner Y, Bengoa-Vergniory N,
            Rubin AN and Kessaris N.
  TITLE     Fate mapping reveals mixed embryonic origin and unique
            developmental codes of mouse forebrain septal neurons
  JOURNAL   Commun Biol 5 (1), 1137 (2022)
   PUBMED   36302841
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3283)
  AUTHORS   DeAizpurua HJ, Harrison LC and Cram DS.
  TITLE     An ELISA for antibodies to recombinant glutamic acid decarboxylase
            in IDDM
  JOURNAL   Diabetes 41 (9), 1182-1187 (1992)
   PUBMED   1499869
REFERENCE   7  (bases 1 to 3283)
  AUTHORS   Oettinger MA, Stanger B, Schatz DG, Glaser T, Call K, Housman D and
            Baltimore D.
  TITLE     The recombination activating genes, RAG 1 and RAG 2, are on
            chromosome 11p in humans and chromosome 2p in mice
  JOURNAL   Immunogenetics 35 (2), 97-101 (1992)
   PUBMED   1735560
REFERENCE   8  (bases 1 to 3283)
  AUTHORS   Huang WM, Reed-Fourquet L, Wu E and Wu JY.
  TITLE     Molecular cloning and amino acid sequence of brain L-glutamate
            decarboxylase
  JOURNAL   Proc Natl Acad Sci U S A 87 (21), 8491-8495 (1990)
   PUBMED   2236059
REFERENCE   9  (bases 1 to 3283)
  AUTHORS   Brilliant MH, Szabo G, Katarova Z, Kozak CA, Glaser TM, Greenspan
            RJ and Housman DE.
  TITLE     Sequences homologous to glutamic acid decarboxylase cDNA are
            present on mouse chromosomes 2 and 10
  JOURNAL   Genomics 6 (1), 115-122 (1990)
   PUBMED   1968040
  REMARK    Erratum:[Genomics 1990 Apr;6(4):708]
REFERENCE   10 (bases 1 to 3283)
  AUTHORS   Maxson,S.C. and Cowen,J.S.
  TITLE     Electroencephalographic correlates of the audiogenic seizure
            response of inbred mice
  JOURNAL   Physiol Behav 16 (5), 623-629 (1976)
   PUBMED   972955
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY265972.1, AK148497.1 and
            AI845043.1.
            
            [WARNING] On Apr 25, 2023 this sequence was replaced by
            NM_008077.6.
            
            On Aug 21, 2015 this sequence version replaced NM_008077.4.
            
            Summary: The protein encoded by this gene is an enzyme that
            catalyzes the production of gamma-aminobutyric acid from L-glutamic
            acid. Alternative splicing of this gene results in two products,
            the predominant 67 kDa isoform and a smaller 25 kDa isoform. The
            smaller isoform is not thought to retain catalytic activity.
            [provided by RefSeq, Aug 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (GAD67).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC027059.1, AK148497.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                BY265972.1         1-37
            38-3235             AK148497.1         3-3200
            3236-3283           AI845043.1         1-48                c
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 41.63 cM"
     gene            1..3283
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /note="glutamate decarboxylase 1"
                     /db_xref="GeneID:14415"
                     /db_xref="MGI:MGI:95632"
     exon            1..204
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /inference="alignment:Splign:2.1.0"
     exon            205..347
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             269..2050
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /EC_number="4.1.1.15"
                     /note="isoform GAD67 is encoded by transcript variant 1;
                     67 kDa glutamic acid decarboxylase; glutamic acid
                     decarboxylase 1"
                     /codon_start=1
                     /product="glutamate decarboxylase 1 isoform GAD67"
                     /protein_id="NP_032103.2"
                     /db_xref="CCDS:CCDS16108.1"
                     /db_xref="GeneID:14415"
                     /db_xref="MGI:MGI:95632"
                     /translation="MASSTPSPATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGL
                     KICGFLQRTNSLEEKSRLVSAFRERQSSKNLLSCENSDQGARFRRTETDFSNLFAQDL
                     LPAKNGEEQTAQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDH
                     PESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMFTYEIA
                     PVFVLMEQITLKKMREIVGWSNKDGDGIFSPGGAISNMYSIMAARYKYFPEVKTKGMA
                     AVPKLVLFTSEHSHYSIKKAGAALGFGTDNVILIKCNERGKIIPADLEAKILDAKQKG
                     YVPLYVNATAGTTVYGAFDPIQEIADICEKYNLWLHVDAAWGGGLLMSRKHRHKLSGI
                     ERANSVTWNPHKMMGVLLQCSAILVKEKGILQGCNQMCAGYLFQPDKQYDVSYDTGDK
                     AIQCGRHVDIFKFWLMWKAKGTVGFENQINKCLELADYLYAKIKNREEFEMVFDGEPE
                     HTNVCFWYIPQSLRGVPDSPERREKLHRVAPKIKALMMESGTTMVGYQPQGDKANFFR
                     MVISNPAATQSDIDFLIEEIERLGQDL"
     exon            348..410
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /inference="alignment:Splign:2.1.0"
     exon            411..569
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /inference="alignment:Splign:2.1.0"
     exon            570..812
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /inference="alignment:Splign:2.1.0"
     exon            813..903
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1016
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1132
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1212
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1213..1267
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1384
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1449
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..1528
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1678
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1679..1786
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1787..1876
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1877..3268
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3242..3247
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
     regulatory      3247..3252
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
     polyA_site      3268
                     /gene="Gad1"
                     /gene_synonym="EP10; Gad-1"
ORIGIN      
        1 gcctccccca agcccagcgg ccgcctctcc ggatctctcc cttcttcagg ctctcccgtg
       61 ccggaccagg gatcgtgcaa gcaaggaagc agccctgggg tgacacccag cacgtactcc
      121 tgtgacagag ccgagcccag cccagccccg ggacgcttcg cagaggagtc gcgggagggt
      181 ccagctcgct gtcgctgaac cgagcctgtt cctgcgccca gtctgcgggg gacccttgaa
      241 ccgtagagac cccaagacca ccgagctgat ggcatcttcc actccttcgc ctgcaacctc
      301 ctcgaacgcg ggagcggatc ctaatactac caacctgcgc cctacaacgt atgatacttg
      361 gtgtggcgta gcccatggat gcaccagaaa actgggcctg aagatctgtg gcttcttaca
      421 aaggaccaat agcctggaag agaagagtcg tcttgtgagc gccttcaggg agaggcagtc
      481 ctccaagaac ctgctttcct gtgaaaacag tgaccagggt gcccgcttcc ggcgcacaga
      541 gaccgacttc tccaacctgt ttgctcaaga tctgcttcca gctaagaacg gggaggagca
      601 aactgcgcag ttcttgctgg aagtggtaga catactcctc aactatgtcc gcaagacatt
      661 tgatcgctcc accaaggttc tggatttcca ccacccacac cagttgctgg aaggcatgga
      721 aggctttaat ttggagctgt ctgaccaccc cgagtctctg gagcagatcc tggttgactg
      781 tagagacacc ctgaagtacg gggttcgcac aggtcaccct cgatttttca accagctctc
      841 tactggtttg gatatcattg gtttagctgg tgaatggctg acatcgactg ccaataccaa
      901 tatgttcaca tatgaaattg cacccgtgtt tgttctcatg gaacagatta ctcttaagaa
      961 gatgagagag atcgttggat ggtcaaataa agatggtgat gggatatttt ctcctggggg
     1021 agccatatcc aatatgtaca gcatcatggc tgctcgttac aagtacttcc cagaagtgaa
     1081 gacaaaaggc atggcggctg tgcccaaact ggtcctcttc acctcagaac acagtcacta
     1141 ttccataaag aaagccgggg ctgcgcttgg ctttggaacc gacaatgtga ttttgataaa
     1201 gtgcaatgaa agggggaaga taattccggc tgatttagag gcaaaaattc ttgatgccaa
     1261 acaaaagggc tatgttcccc tttatgtcaa tgcaaccgca ggcacgactg tttacggagc
     1321 attcgatcca atccaggaaa ttgcggacat atgtgagaaa tacaaccttt ggctgcatgt
     1381 ggatgctgcc tggggtggtg gactgctcat gtcccggaag caccgccaca aactcagcgg
     1441 catagaaagg gccaattcag tcacctggaa ccctcacaag atgatgggcg tgctgctcca
     1501 gtgctctgcc attctggtca aggaaaaggg tatactccaa ggatgcaacc agatgtgtgc
     1561 aggctacctc ttccagccag acaagcagta tgacgtctcc tatgacaccg gggacaaggc
     1621 gattcagtgt ggccgccatg tggacatctt caagttctgg ctgatgtgga aagcaaaggg
     1681 caccgtggga tttgaaaacc agatcaacaa atgcctggag ctggctgatt acctctacgc
     1741 caagattaaa aacagagaag agtttgagat ggttttcgat ggtgagcctg agcacacaaa
     1801 tgtctgtttc tggtacattc cacaaagcct tcgaggggtt ccagatagcc ctgagcgacg
     1861 agaaaagcta cacagggtgg ctcccaagat caaagctctg atgatggagt caggaacaac
     1921 catggtgggc taccagcctc aaggggacaa ggccaacttc ttccggatgg tcatctctaa
     1981 cccagccgcc acccagtctg acatcgattt cctcattgag gagatagaga ggttgggtca
     2041 ggatctgtaa tccctcttta cagaaccaga atcatcggcc atgcttgtgc ccctctggta
     2101 ccctagagca cagctctgtc agtagctgac acatctaggc catctcactg aaggaaatta
     2161 caatctctta aagaatattt gtcacatttc acataagctt gtttgttaga gctagtgggg
     2221 aaataatgtt ctttttaaaa aattgcacat tagaaacaca gtatatatgt acagttatat
     2281 atacctctct ctatgtacat gtatgtatag tgactgtggc ttagtcctag accatagcat
     2341 gttgctcgtc cccaggaaat tagccttacc cccagcaatt actaagaggc taaaccatct
     2401 cgcaagcaac tacagggcgg atggtatccc tactgcagtg tcctagggac ccagggaaag
     2461 gctgttggtg ggaggctacc tccctgctag agctgttccc acgtgaaggg atgatggatg
     2521 acagatgtac cagtaaatga caaatgtcac accctccctg ttagtaccct gctaggggaa
     2581 atagtagcag tctttgtcac aattgtgctt tgctgtgttt tagagattaa tctgtgtaaa
     2641 ctgtgtacat tgccattgtc tatctttggg cagggggaag tgcatataat gattaaattg
     2701 tatgtcagtg agatatctgc ttatttatat tcaaatatat accatgttaa agagacatct
     2761 tgtattttct tcccatttgt aatgtatctt atttatatat taatggagta agttctggat
     2821 actgtttatg gtattttcgt gcatttgtga gccaaagaga aaagatgaaa attagtgaga
     2881 tttgcattta tattagagtg cccttaacat aatgatttga acatatgtgt actgtctgga
     2941 aaagaattct gatactgtac atagagtcat gttatggaaa tcttgcttca gtagccttcg
     3001 ctcttctctt tcccccctca ggctgtatgt cagatgttct caaagctttt ctagtaactg
     3061 ttgaataata ataactagat ctcctgtaat tttgtagtag tatatgacca atctctgtga
     3121 ctcgcttagc tgaaacctaa ggcaatgttt ctgaagacct ccgatacact gaccagtccc
     3181 acaagtgttt ttgaagacat gaaacccaca ctgtgcattt agggtgtgca agaagaatat
     3241 aaataaaata aaaaatattc tccatgaaaa aaaaaaaaaa aaa
//
