LOCUS       NM_008069              14272 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus gamma-aminobutyric acid type A receptor subunit beta 1
            (Gabrb1), transcript variant 1, mRNA.
ACCESSION   NM_008069
VERSION     NM_008069.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 14272)
  AUTHORS   Zietek,M.M., Jaszczyk,A., Stankiewicz,A.M. and Sampino,S.
  TITLE     Prenatal gene-environment interactions mediate the impact of
            advanced maternal age on mouse offspring behavior
  JOURNAL   Sci Rep 14 (1), 31733 (2024)
   PUBMED   39738558
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14272)
  AUTHORS   Blednov,Y.A., Shawlot,W., Homanics,G.E., Osterndorff-Kahanek,E.A.,
            Mason,S., Mayfield,J., Smalley,J.L., Moss,S.J. and Messing,R.O.
  TITLE     The PDE4 inhibitor apremilast modulates ethanol responses in
            Gabrb1-S409A knock-in mice via PKA-dependent and independent
            mechanisms
  JOURNAL   Neuropharmacology 257, 110035 (2024)
   PUBMED   38876310
REFERENCE   3  (bases 1 to 14272)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 14272)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 14272)
  AUTHORS   Lie,M.E.K., Falk-Petersen,C.B., Piilgaard,L., Griem-Krey,N.,
            Wellendorph,P. and Kornum,B.R.
  TITLE     GABAA receptor beta1 -subunit knock-out mice show increased delta
            power in NREM sleep and decreased theta power in REM sleep
  JOURNAL   Eur J Neurosci 54 (2), 4445-4455 (2021)
   PUBMED   33942407
  REMARK    GeneRIF: GABAA receptor beta1 -subunit knock-out mice show
            increased delta power in NREM sleep and decreased theta power in
            REM sleep.
REFERENCE   6  (bases 1 to 14272)
  AUTHORS   Sommers,C.L., Huang,K., Shores,E.W., Grinberg,A., Charlick,D.A.,
            Kozak,C.A. and Love,P.E.
  TITLE     Murine txk: a protein tyrosine kinase gene regulated by T cell
            activation
  JOURNAL   Oncogene 11 (2), 245-251 (1995)
   PUBMED   7542761
REFERENCE   7  (bases 1 to 14272)
  AUTHORS   Haire,R.N. and Litman,G.W.
  TITLE     The murine form of TXK, a novel TEC kinase expressed in thymus maps
            to chromosome 5
  JOURNAL   Mamm Genome 6 (7), 476-480 (1995)
   PUBMED   7579892
REFERENCE   8  (bases 1 to 14272)
  AUTHORS   Kamatchi,G.L., Kofuji,P., Wang,J.B., Fernando,J.C., Liu,Z.,
            Mathura,J.R. Jr. and Burt,D.R.
  TITLE     GABAA receptor beta 1, beta 2, and beta 3 subunits: comparisons in
            DBA/2J and C57BL/6J mice
  JOURNAL   Biochim Biophys Acta 1261 (1), 134-142 (1995)
   PUBMED   7893750
REFERENCE   9  (bases 1 to 14272)
  AUTHORS   Zdilar,D., Luntz-Leybman,V., Frostholm,A. and Rotter,A.
  TITLE     Differential expression of GABAA/benzodiazepine receptor beta 1,
            beta 2, and beta 3 subunit mRNAs in the developing mouse cerebellum
  JOURNAL   J Comp Neurol 326 (4), 580-594 (1992)
   PUBMED   1282920
REFERENCE   10 (bases 1 to 14272)
  AUTHORS   Buckle,V.J., Fujita,N., Ryder-Cook,A.S., Derry,J.M., Barnard,P.J.,
            Lebo,R.V., Schofield,P.R., Seeburg,P.H., Bateson,A.N.,
            Darlison,M.G. et al.
  TITLE     Chromosomal localization of GABAA receptor subunit genes:
            relationship to human genetic disease
  JOURNAL   Neuron 3 (5), 647-654 (1989)
   PUBMED   2561974
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC114826.6, AC101688.13 and AC122915.2.
            
            On Jun 18, 2019 this sequence version replaced NM_008069.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC157920.1, SRR7345562.3293623.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-164               AC114826.6         130858-131021
            165-256             AC114826.6         131215-131306
            257-324             AC114826.6         131731-131798
            325-545             AC101688.13        32179-32399
            546-628             AC101688.13        192607-192689
            629-766             AC122915.2         109871-110008
            767-919             AC122915.2         110247-110399
            920-1164            AC122915.2         123512-123756
            1165-14272          AC122915.2         138028-151135
FEATURES             Location/Qualifiers
     source          1..14272
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 38.18 cM"
     gene            1..14272
                     /gene="Gabrb1"
                     /gene_synonym="B230208N19Rik; Gabrb-1"
                     /note="gamma-aminobutyric acid type A receptor subunit
                     beta 1"
                     /db_xref="GeneID:14400"
                     /db_xref="MGI:MGI:95619"
     exon            1..164
                     /gene="Gabrb1"
                     /gene_synonym="B230208N19Rik; Gabrb-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             85..1509
                     /gene="Gabrb1"
                     /gene_synonym="B230208N19Rik; Gabrb-1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; gamma-aminobutyric acid receptor subunit
                     beta-1; GABA(A) receptor subunit beta-1;
                     gamma-aminobutyric acid (GABA) A receptor, subunit beta 1;
                     GABAAR subunit beta-1"
                     /codon_start=1
                     /product="gamma-aminobutyric acid receptor subunit beta-1
                     isoform 1 precursor"
                     /protein_id="NP_032095.1"
                     /db_xref="CCDS:CCDS19329.1"
                     /db_xref="GeneID:14400"
                     /db_xref="MGI:MGI:95619"
                     /translation="MWTVQNRESLGLLSFPVMVAMVCCAHSSNEPSNMSYVKETVDRL
                     LKGYDIRLRPDFGGPPVDVGMRIDVASIDMVSEVNMDYTLTMYFQQSWKDKRLSYSGI
                     PLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLYGLRITTTAA
                     CMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWNGGEGAVTGVNKIELPQFSIVDYKM
                     VSKKVEFTTGAYPRLSLSFRLKRNIGYFILQTYMPSTLITILSWVSFWINYDASAARV
                     ALGITTVLTMTTISTHLRETLPKIPYVKAIDIYLMGCFVFVFLALLEYAFVNYIFFGK
                     GPQKKGASKQDQSANEKNRLEMNKVQVDAHGNILLSTLEIRNETSGSEVLTGVSDPKA
                     TMYSYDSASIQYRKPLSSREGFGRGLDRHGVPGKGRIRRRASQLKVKIPDLTDVNSID
                     KWSRMFFPITFSLFNVVYWLYYVH"
     exon            165..256
                     /gene="Gabrb1"
                     /gene_synonym="B230208N19Rik; Gabrb-1"
                     /inference="alignment:Splign:2.1.0"
     exon            257..324
                     /gene="Gabrb1"
                     /gene_synonym="B230208N19Rik; Gabrb-1"
                     /inference="alignment:Splign:2.1.0"
     exon            325..545
                     /gene="Gabrb1"
                     /gene_synonym="B230208N19Rik; Gabrb-1"
                     /inference="alignment:Splign:2.1.0"
     exon            546..628
                     /gene="Gabrb1"
                     /gene_synonym="B230208N19Rik; Gabrb-1"
                     /inference="alignment:Splign:2.1.0"
     exon            629..766
                     /gene="Gabrb1"
                     /gene_synonym="B230208N19Rik; Gabrb-1"
                     /inference="alignment:Splign:2.1.0"
     exon            767..919
                     /gene="Gabrb1"
                     /gene_synonym="B230208N19Rik; Gabrb-1"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1164
                     /gene="Gabrb1"
                     /gene_synonym="B230208N19Rik; Gabrb-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..14272
                     /gene="Gabrb1"
                     /gene_synonym="B230208N19Rik; Gabrb-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      13714..13719
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gabrb1"
                     /gene_synonym="B230208N19Rik; Gabrb-1"
                     /note="hexamer: AATAAA"
     polyA_site      13729
                     /gene="Gabrb1"
                     /gene_synonym="B230208N19Rik; Gabrb-1"
                     /note="major polyA site"
     polyA_site      13737
                     /gene="Gabrb1"
                     /gene_synonym="B230208N19Rik; Gabrb-1"
     regulatory      14250..14255
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gabrb1"
                     /gene_synonym="B230208N19Rik; Gabrb-1"
                     /note="hexamer: AATAAA"
     polyA_site      14272
                     /gene="Gabrb1"
                     /gene_synonym="B230208N19Rik; Gabrb-1"
ORIGIN      
        1 attcgggaat tactgcccag ccgccgacta agttgcattc cttgaatctt cgcagaaaag
       61 acaattcttt catcagagtt aggaatgtgg acagtacaaa atcgagagag tttggggctt
      121 ctctcttttc ccgtgatggt tgctatggtt tgttgtgcac acagctccaa tgaacccagc
      181 aacatgtcat acgtgaaaga gacagtggac cgactgctca aaggatatga cattcgcttg
      241 cggccggact ttggagggcc cccggtggac gtcgggatgc ggatcgatgt cgccagcata
      301 gacatggtct cggaagtgaa tatggattat acactcacca tgtatttcca gcagtcttgg
      361 aaggacaaga ggctttcata ttctggaatc ccattaaatc tgacccttga caacagagta
      421 gctgaccaac tctgggtacc agacacctac tttctcaatg acaagaaatc atttgtgcat
      481 ggggtcacag tgaaaaaccg aatgatcagg ctgcatcctg atggaactgt tctctatgga
      541 ctacggatca caaccactgc agcatgcatg atggatcttc gaagataccc tctggatgag
      601 caaaactgca ccctggagat tgaaagttat ggctatacca cggatgacat cgaattttac
      661 tggaatggag gagagggagc agtaactggg gtcaataaaa ttgagcttcc ccaattttca
      721 attgttgact acaagatggt gtccaagaag gtggagttca caacaggggc atatccacga
      781 ctatcactaa gttttcgtct aaagagaaac attggttact tcattttgca gacctacatg
      841 ccttccacac tgattacaat cctgtcctgg gtgtcgtttt ggatcaacta tgatgcatct
      901 gcagccagag tcgcactagg aatcactacg gtgctgacca tgaccaccat cagcactcac
      961 ctcagggaga ctttgccgaa gatcccttac gtcaaagcga ttgacatcta tctcatgggc
     1021 tgttttgtgt ttgtgttcct ggctctactg gaatatgctt ttgtcaatta cattttcttc
     1081 ggaaaaggcc ctcagaaaaa gggagcgagc aaacaagacc agagtgccaa tgaaaagaac
     1141 agactggaga tgaacaaagt ccaagttgat gcccacggca atattctcct cagcaccctg
     1201 gaaatcagga acgagaccag tggctccgag gtgctcacag gtgtaagtga ccccaaggcc
     1261 accatgtact cgtacgacag cgccagcatc cagtaccgca agccactgag cagccgcgag
     1321 ggcttcgggc gtgggctgga caggcacggg gtaccgggca aggggcgcat ccgcaggcgc
     1381 gcctcgcagc tcaaagtgaa gatccccgac ttgactgacg tgaactccat agacaagtgg
     1441 tcccgaatgt tcttccccat caccttttct ctttttaatg tcgtttattg gctttactat
     1501 gtacactaag gtcagtctcg tggttcggtt tagactacct tcctcctcgc ttgtttttta
     1561 accccacaga ttcccaacag cggtaactgc tgtggttttt gaggtaagag attcagcctt
     1621 ccagttggtt ttcgatcttg tgtgtcagtt tttattatca caccatgttt acttcaacaa
     1681 caacaacaac ggcaacaaca aaaggtattt tcctgtcaac tgtggtccaa ggtacgagct
     1741 ctgtaagagt tccaccaatt gctttgttta caaacacaaa gagagaggtg gctaggtggt
     1801 agatattgtt ggcagtcttt ctcagttacc ctggatctta ctggattatt tttaaatgaa
     1861 aatgtacagt ggaccttgct atctacctgc actgtgctct gggtaaactg taaccatctc
     1921 atgatgccaa aaaaaattaa atagaaatta aaaaaaaaaa aggaaaaaaa aaaaaaaaaa
     1981 gaaaacaact tgagcatctc agaagaataa agccattgat tgacaagttg cagatttcca
     2041 ggaagagggt atataaaact gtcagttccc atcctctcta agttcttctg agccctttcc
     2101 agaaaggctt cggagctccg tggaggagga acattaaagc ttgtgcttct tcccactttt
     2161 ccccaaactc accctattgt gtccatctga caatggccat ggctagtcct aacaatggtc
     2221 ctctctcagc ctgcctagat gttcaaagtc aacattagaa cccagcctga tctgactggt
     2281 agtcactgaa accatttttc tttacttctc aagagtaaag aattggggaa aacgatagtt
     2341 ttccttgctg tctgttatgg agagcagccc cgactgccct tcatgggcct tggaatcgct
     2401 tgatgacatg aaaacatatg atcattttcc acagttcctg cttcagtgag aaggaaagga
     2461 tatatagctg gtgagggctt taaaggagga ggagaccaat tttttccttg aggggaaaaa
     2521 aaaaatgcta tttccctgaa gaaggcaaga cttacatgtt atgtaaaagg tgcctgggca
     2581 gttatctgca ctgccaaatc ttaagtcgaa ttgtatgtac ctcttagcct tccagagagc
     2641 agtgtctaaa cagcggcgag ctcttcacat aggactctgt gggcactttt tgcatctgct
     2701 ctctgaactc tgcatttcaa gctacaaaat gaatggcccg ggaaatgctg accctccaca
     2761 tcaatgtctt cctccaaagc cagatttcca ggttaagaaa agctttaaag tatagccaaa
     2821 tcacagaaag aaaagaaact ctgaaatggc aacacactga gcctttagca acgcccacca
     2881 gcccctgagg cgtggaccgg aagtgtctcc actcattcac cactgtcatt atggcactgc
     2941 cccatctcgc tcgtggatta ttctgggtcg tggttctcat gactttcagt aaagcaacgc
     3001 aattctaaac atgctcaata agctactgtt gagttcttcc cgtttttgca ggattaaaat
     3061 ctcattttcc tttggtctga gttgtaaaat tatctgttta tttgcaaggc tgagtgagag
     3121 cgcaaattga ccagccgtgg atggaggcca tgttggcacg atccaggcat ggaactgcag
     3181 ggctaatcag gatgacagga tgactgcctc atttgatgct ctggccctcc tctgagaaga
     3241 gaatcggtgt gcagagtagt gtgagctgct aacaattata gtcacaattg aaaaggaaag
     3301 gctagaattt atattaagga gcccaagagg agaatataaa atattcattt agaaaacaag
     3361 aggaaaagag aatccttaaa taagtgaatg tggctggatg tactaacaga cattagtaaa
     3421 agtatcaaaa taccttaaac aaaacaaaac aaaaaactga tcacagctat ttcatgacga
     3481 gtgctaggat tttgaaggca aattttcagt taggttttca ggtgtgtgaa cacacctccc
     3541 tagttgaact aacatcatcc tggggtcaaa gggtcatctt tgtaaaaagc tctgctcagc
     3601 ttccagcaag ccaagatgct ttgccatgga caatccctgg acacaataga acgtgatcct
     3661 gtcagtagtg ggaggctctt aatacccaag agtcactaag cagtcgctga ttttaaacac
     3721 aggaatggat attaggtgcc tgatgctgaa cagacagcaa tcattctgat gtcaaagatc
     3781 tttaagtggc tttcaagaaa aaaaaactaa atcagaaagt gtctacttag atcttctcat
     3841 aaaagactgg gatagtttat gttttcagca ctttataaaa tctaaagtga ttttgttata
     3901 gttttagata taaagcatct tttgcattaa aaaaaaagga aacaacattt tgaacttttc
     3961 ctacatttca acctttcaaa atgtaaaatg attaggcctg aattgcccac tgggcatgga
     4021 atggtatgtg gatcacagcc accattatgc aaatctgact acaaagcatt gatctcagag
     4081 accatagaca cctgaagcta ctattgcatc aaggaaagat gtcttgcttg tgagtcatga
     4141 tctttgtcct accttctgaa cacctcccag actaatctgt tcttctctgc tcccttccca
     4201 agccaaacac tgacagggaa gcaccttcct tctcagattt tctccctctt ctttcccaaa
     4261 aatgatgtaa ataaaacata aaagtcataa aacattcata aatgccctca catggcccta
     4321 ttcttgaaag actcagatta cattatacag aggttgttat ttaactatta ttgagtcaag
     4381 ttccttttgc tatgaaaata gccataatcc cctgcaccaa ggagcagaag agactgaaaa
     4441 ctgcacactt cctctcttat gcctcattta catccagaga accctatgtt tcacagtttg
     4501 acagtgggac agatcatgtg gaaaacttaa ccggctgtgg gcacagtaga catctgcacc
     4561 tttttcttga caactgtgac tgctcataaa acatcaggta ttccttctgc ttggcaattc
     4621 tgtgaggcag agacccaggt aaaggcttat ttctgcttga ctgatccagg attagtatct
     4681 caatctcctc tgttgccaaa cagcaacagc agttggctgg caagcctgct gaatgaataa
     4741 tctgtgtttg tgtacacatg cataaagtat atctatatac tttattgtac actacaatca
     4801 atgatggatt tttaaaaatg agaaaaatat ggtgcagaga aaaggagtaa tggctttagg
     4861 gtcctaagaa aaatggactt tcctttaaga aagccataga aaattcacaa aagttccatt
     4921 ttcctaaaac caatgactat ttatgaatat tcattcagaa ggggtttgat tacaatctgc
     4981 caagttacat gaaaaggaaa caggataatc tttctagaaa aaaaaagtct atgagattat
     5041 tcatgtctaa aattttggac aatatcttgt caaaggggtg atgtgccacc atttgtttct
     5101 taatgcataa tcatttatcc tagggagggg tgaaaggagc gtgcaaccac ttgctcaagg
     5161 tggttcaatc agcagtttcc tgaatgaaca gaagagaaaa acacccatat atcggaaaga
     5221 aaacacaaag gtccatttgt taatggaatg gagaacactg tgtaaacttc cttgtaaatt
     5281 ctgtacatag ttcactgaac tgtcccctgt ggtatggtgg tcctcaactt ctgggtggat
     5341 gtttggaaaa ctgaagcact gtaaatatca tacgcttata aagcctttgc tttttattca
     5401 tgaggtgaaa tgattagtga ttcgggccca aaccagactc tctccagctc ttgctctttc
     5461 attcgtacag ttgtgtgact ctgctgtaag ccatagccag gtgtctgtga tcactgcctc
     5521 atgcctttac ccactgacag ggacagcagc tccctagaag gctgccaacc tgcggggcca
     5581 aagaacagag caccagcaga aaccagaact gggacaatct gctccttgag cagtcattta
     5641 aaaaaaaaat agataagttg ttttcagtca ctgtggctat ttgtatcatc aagaactgaa
     5701 tagccgatgc tgcttaagga gtcagggatg gaagcgatgc tcaaattcca cagggaatcc
     5761 aaagcttcat ttttgccaat agctttcact tctaacgttt ccactttgtc ttattgaact
     5821 tctagcaaaa ctaaacatga atagaacgca tgggtctgat cagggtgaac agtagttacg
     5881 caaaggatag gcagggggta caggtcgaga cgataaatgg ccttgatagt gctgggtggc
     5941 agattcatac gtgagagata caggggtatc tgtgttctta ctcttaaagg tagacaaaag
     6001 tttctgggtg tgaactgata gagaaggcaa acgagggaat ttaacttgag aaagggttgt
     6061 gtatggtgga agctacttga gtctgaagaa cctggtaact gaacgattcc aggtattgct
     6121 ccctccaagt tttcagagtc ctttcaaaag tctccagcgt gtgggctgtt acagcacaga
     6181 acgcgcttcc atgcatgcta gcaatcccag agaggcaggc agcaccaaca gaaattcaga
     6241 ctctccaagc aattgaagac agagattagt ttcttagtgc tagaggggaa ggtgtcctcc
     6301 gtgcaggtaa gattctttgt gtcattttaa gtgttaggag agtacacacg gggagccatt
     6361 actttggaac attttaatcc caagatgtga cccaccaccg gctgaacaca caaaacacac
     6421 cttgttggaa ctaatccatt ctgccagttt ccaggctaga acatattgtg atttttacct
     6481 tagcttttct tcagatagca tattatctgg aatgattgaa aatgtaatgg ttgaaagctg
     6541 cagcaagtgt ttgatagaag ttctttttaa taaaagaatt acaaaccaga aaatattaaa
     6601 ttcctagcag ggtaaaaata agggttttct gtttgttttg acatctccca tggcagacat
     6661 ttcccattaa aattgaagaa gtctaacagg attttcttct tgtttttctc ttaccttttc
     6721 agaagccttt ggcttattaa gagactcaca gcaagagact cagaaccatt tttgattgac
     6781 tcccatacct tttgcaattc gtgaatttat caaaaaaaaa aaaagtccag tgggaattca
     6841 agttgaaaaa aattgaaact cctgaaccaa ttgagaagga gaaggaattc cactctccag
     6901 agctgcacta cctgtacatt acgtcttgtg gacaatcaga ccaagtcagt ccagacaaga
     6961 gaggccaaaa acccttgttc atttgcctta gggacacaga gttacagatt ccttcattaa
     7021 tccagtctca gtccaaagcg aaaggatgga caggcgcagt attatttatt acctggctct
     7081 tgcttcttcc caggcttcct gggaggagaa acaggaacca ggagacactc tcaaatatat
     7141 tttatatatt caaataacta aaatattata tgtatatttt gcaatcttgg tttttataaa
     7201 atagatgaaa tcttctgtct tatttcccaa ctatacaccc aatggaaaca attctattcc
     7261 tctatttatt cttcatttca tttctgaagc ttatatgcta tcttgctcat acttagacgg
     7321 cagcttcaat ctttggactt acattgtatt tcttcattta actcatgaac aatgaattgt
     7381 aacaattctt cccaattcct actcatggta cctttaaaag gtcttcagag gcaagacttt
     7441 ttaaccacct ctgaatgccc ttgaattatt tttctttcgg ttctttatgt aaagagtaca
     7501 cacatttaca aaagtcttct ctctccaaac atatttcaaa acacagaaga aattaaaata
     7561 atgcacttag acctatggtt gattacatta tctataccag aacttaaagt ttcaagacat
     7621 catggtagca tgataaattt atgtttttct atgcataggc tcccctagtc ttaactatat
     7681 ggcatttaaa tttttcctat tattcttccc agaatctctt atcactcaag ttgtacaaca
     7741 gtggttatag ataatcattt aggaattgga cctatttcat catttctgta catcatgcta
     7801 gacttacact tcatatattt taccttgttt actcccccca tctcaacctt ctagcagatt
     7861 ctacaaagtt agcaattgca aaaaccatta tgcgctgtcc aaattatcac cagagaattc
     7921 tgtaagatgc atttcctcca taaagcatct ctatagactt agagatatat gatatgtata
     7981 gacagagttc tctaaacata cagcaaacaa catgccctat aatattgaaa gagtaacagc
     8041 atgtggtgaa tagagccagg agataagatg aaagacacct tggaatgagg atgggggtat
     8101 gtgtctaccc aggactgtga ctcttgtcat gcccactgat gacttcaagt tctctttcag
     8161 aaccaagttt caaaggttat tttagagagg ctcatctact ctggagttta accatatgat
     8221 gcgggttcac catagacctt gttcatgcag cctcacaaat ctaataatag ccttagtctc
     8281 atgcctttaa gattagccac atggtgtcaa gtgaactgag tagtagtcat atttcttcca
     8341 aacctctgct tttaaaatat cctatgtcac aatcttccat ctcccatctt agagaagtat
     8401 ctgtaaggga ctctcacaca tcattcaccc atgtgatatg gtctccaatt ttacatcttg
     8461 ggaattttag atgttgttaa accatcctct gtaacttagc caagaaaaag ccgtacgacc
     8521 ctccctcgtg gatggaaggc catcttctag gcagtgtctg agcccaaatt cattccagac
     8581 atatccagtt ttaaacctca catctctcta tgtctctaaa tgacatgagc aaatgtattt
     8641 gagctttcac gccagatgca actacccatc tagatgaaag agactctgag ttcttaaaag
     8701 ctgttacaca gccttcctgt ccttgtcctc ttgtctcctg ctacactact tgaacaatca
     8761 aattggtctg atattttaga tacagccata gcaaaaagtt aattaagtgg tgattggcct
     8821 cgcagttgtt ctccagccag cgcacatgac aaccaaagta atttctgcaa gtatccttaa
     8881 cttttactga gtgtttatga ttctcctggg agccttctaa ctgttgaaaa ttgttaggat
     8941 aatcttttcc catccaattg cctatgtctg tggatctgtc atatatggca ttaattgtgt
     9001 tcatgtgtgt ttcttctata tattgaaaga ttttatttat tatattgaat tttttaaaaa
     9061 taattgttag gataacgcac agaaatgcca tttttcggct gtatttcatc ctatgaatag
     9121 aacaccttct cttttgcaaa attagctctg ctcccagcct taggcaacct tcaaagatga
     9181 ttctcccaac ctccccatgt gttgatggac tcctgctcct cttcaagcaa tgacgtggtt
     9241 gctttctttt ctgcttcccc atgatgtttt taacctttag acggagtcac acctcacaat
     9301 atcattttta gagggaggaa gtgtttaagt cgtaaccgag gtgaagttag tatttactaa
     9361 tgtaggtcta tctactgaga cccattcagc tacaggtaac aaaagatgac tctcagagga
     9421 tcttacgcaa tcaaaacatt ctaagctcat ataacaagat tagaagtgcc tagtctagcg
     9481 tgcttatttc aagaatataa gacccaagac tcttgtctga ttctctgaat attatcagca
     9541 ctcagtacat ctgcaacaag gttgcacaaa ccatccaaga tattcagaaa cagggcaagt
     9601 cctggattat tttttaaagg caaggaactc tttcctcaac cagccccaga agccatcctt
     9661 gtcctgttga ctgaggttga gtcaggcatc attcctcaac catttactga agagtatggt
     9721 tatcataaca ggcttacaag tacccagtgc tacacaacat aattgattga taaattaatt
     9781 taaaatgaaa ctaagaatgg ctttgggtag ctatccaata ataactctgt catattagac
     9841 taagggaaaa tactctccca ctataccata gcacgtcact ctacactcca gccctatgga
     9901 gttattcaat aacaccagtg tcgggacaca gaaacccgaa tagcttaagt aacttaccca
     9961 gatctgatgg ttactggtga agtgggtatt cattgccagt ttttcttgcc tccaaagaca
    10021 aagtttctac ctctcccaca tgatctatat tgaatccctc tgacatcaat aatacaggct
    10081 ggtggagtag ttcaaggaag tgcaaaaact tggatgaagt cagctcttac agccaagagt
    10141 aagatcctgt ggccaccaga cactgttcag tttatttcta tccgctctcc ccactcctca
    10201 ctccccacat aaaagccttt cactgtgcaa gggaagtgag gaagaaaagc cctcgcagta
    10261 gcagctccag ctctgctcca ctctttaggt tcacaccatc attcttagaa gaccgtacat
    10321 cccagtgtgc ctgggacacc cattggagtg acaaattaag tgaccacttt aattatggga
    10381 tctcagcaac ccagaggata tgggaagtac caataggtcc actgatggag cttgtattca
    10441 gtcccagaaa atatgattta tcccttgtga gccacatctt gtgcctaagc aggcttcatc
    10501 tctcctgtgc actaagcttt tcattgctct ctttaattgg ctccttcaag actgacccat
    10561 agccctttat tagccttcac tccgacagcc aggcaccatc accaaatgct ttttattgct
    10621 gcccttcaaa ctttacatct tatggtcagg attccacctc gatgataagt tgcaagccag
    10681 cagcactcaa aatatccatc tgagtaccca tcttcattcc ctcccctgct ggcacccatc
    10741 acttctggaa acaactctgt ccactgtgat gggatagtct gtgtgtttcc agtactcttg
    10801 taaatatttg gcctcaaaaa caaaatagtt cttaagagta aacaccctag tttctaagtc
    10861 ttttgtagga ccccatgcat acaatctcta aataaattta aaaataagat tatacttaca
    10921 gtcgagctct atctgacccc atacggagta gacactggga tatttttttc cccactgact
    10981 gtgagtgagc agacctactc tttataatac ctccactcta cacgagctct agcagaactg
    11041 aattgtccat cctaattact tctgcttttc ctctatgggg aaagggagca tttttctccc
    11101 atagtttcat ttctacccct tctaatccat gcctgcctta ttaaaagtct tttgaatgta
    11161 tgttctgtgg ggcattggaa gttgaaacag cattggattg tcaatggaac actccaatgt
    11221 gcagtcttaa gagaacagtc ccagaggata tttgcacagc aagtaccact tcttctaaga
    11281 tgttaataga agacttggca cgggctagga aggatagaca gttcttaatg ttcaccactg
    11341 ctactcagtt cattgaaaag caggtaatag gaacaacctt cttgtgaccc ttgggatgga
    11401 tggcattcct gctgtgctgt tacatgtttt cctaaaaaca gaaaaagaaa aagaatagac
    11461 tttgccaatg taacccccca aagacaacca gacaccacgg atttactagg tagtgtgttt
    11521 ttctacgtac attttcctca atacatcgaa ctgaagtgtg tttgacctcc ctcatttttt
    11581 tagcaacaca ttaatatctg acttctttat ctttttaaaa aaatattcac tggagaaagc
    11641 ttgaaatgtg tttttcagta gtgtccttga gatattttta ggcgagtccc tggaccattc
    11701 taatcactgt agtgagaatt atgtatccaa gtgacctttt atattcagaa cacccactat
    11761 ttttctaaag gaaggaatat ttatgatttt atacatgtgt atttattcac tgttgctcag
    11821 tcccatattt taaaataagc caataattct cttgataaaa gctaccacgt gacactttta
    11881 aaaggtcaag actgcctgag gtttttgaaa attccccatg gtttacacgc ttgcagggtc
    11941 caaaaatatg ctttaacttt ttaaaataag cttttggttt ccaccaccag gtagcatttt
    12001 gtgccactgt gatgcagatg tgatgctggt atatcattca gaaggttaag ggagggaagg
    12061 gggagtctat agtgctggat aaacaaaagc aaactgtttg agagaaaagt atatagacgg
    12121 aagatgactt actcaaaagg aagaaacaaa acatacaaaa caaacaaaca aacaaaacct
    12181 ctcctggctg cctatcatct ctgaaaaaga aaaaaatctg aatttaacaa aggggcagaa
    12241 tctgaatctg tagcaaaagc ttgccacagt tgtgcggggt gagggtggga gagaggggag
    12301 ctgggaactc aagtcccatt aaagggttca aaatgaccgc tgccggcagt gattcaaaca
    12361 cctcattggc aattacttca ctgaaaactc gactgaatcc aaaactcacc caaacccacc
    12421 aaaccagcgt ctgctacttt aattcacagc accctgcgtt cgatggcctg tggcttccaa
    12481 agcaggtgcc acatccacgc aggaagaaac acacaccttc tgttacttcg cagactctgt
    12541 taagctaagc tcaccaaatt gtgtcagagg acatcaagcg gtttgtttgg tttttctttc
    12601 tttctttttt ttcccccttg tgtttttcag tctccactaa tcaattttgc aatctaccct
    12661 cttgccaaat gtaatttagc aaactcgtta tcctgcttgg cattacaccg gttcttgtaa
    12721 acgaaagaat tcctctctgg ttttcagaag gcatacttct tgcattgcaa aaaaaaacac
    12781 aaatatttaa tgatcttcta ctctttggca tggccttttc tttctaacac gagctggtaa
    12841 tactttctgt gtatgtatca gaccactaaa gaaactcaat ggcaaggaac agtatttctg
    12901 aaaacctgta gaaatgtttg ttgtaatgaa atggcacagg cttaaagaga tgccaagaaa
    12961 ggaaaagatg gactccttac ttttataact caagatttct attttgtatc aatatatgct
    13021 gcattttctg actgaaatcg ctgtctaact ggatttgtgg ccttctgtcg ctttttacat
    13081 ttgaaatcaa atttcataca gtaagcttat ttgtcccttt ctgtttggga gtcaattaga
    13141 atatttcatt tcattaattt atttttttac gtgctaaaac aaaacaaaac agaagcaagt
    13201 gacatttgcc ttcattatct caaaagctat ctgtggacaa aatccatgaa gcctatggac
    13261 ggtgatcaat acaaataaaa ggcagacctc cctgtaaacg gtgaatttgt gaatgaggaa
    13321 ttgttcaatg gcctctgagt ccctcccctc tgtcccttca tggccttctt tctattctga
    13381 gtcatccatc aagcaattgt aagatacaaa atcaaatgac atgctatcag gtatggacac
    13441 aagaaatgct tgtgctctca atttcaggac tcacagctcc ccacagtcac ggggctgtcc
    13501 ttcactcggc ctttttgtta acagtgtggc tttgctagaa aacatgctat cgtttgcttt
    13561 ggtatacaag attttaccct gttgcagcaa atgaaaggca gatggcgcat tggaaaggct
    13621 ggtgtcagat gccatcagct ctgtttggaa ctacaaagct gctgaaatgg aatttactgc
    13681 tgtaaatatg tacaatttat gtcagcttat ttcaataaaa aaaatttaag aaaccaattg
    13741 caagtggctc ctccacgtaa aaaggctact ttgtactgtg ctttaaaaca tgcgagattt
    13801 caggcctatc tttttataat cctttaaaat gtttagaatt cctgtttgac agcttttatt
    13861 gtttaaaaaa aaatcaatac aaagctgagg ccctgtatct aaaggagacg ctttgcaaga
    13921 ttatccaaca actgggggaa aaaacagaag acaaggcagc actttagtgg gagtttgttt
    13981 ctgcactact tttaaacaca ttctttagga aacatgcctt ataaacacga acaggagcat
    14041 ttctatagta gatgggagaa agacatgaac ggtctctcct tgtattaaaa ctgatgtcta
    14101 aaaccaagtc agtaggtatc cacttgtgac atggctcgtg tcaggctttt ctttttaatc
    14161 tctactgtac acatgactat gagagctgtc tgtccatctt tcctcatttg aagacctctg
    14221 gtttccaaac tccttgagaa actattgaca ataaaataac gaacagactg ca
//
