LOCUS       NM_010251               4123 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus gamma-aminobutyric acid type A receptor subunit alpha
            4 (Gabra4), transcript variant 2, mRNA.
ACCESSION   NM_010251
VERSION     NM_010251.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Shigesawa,I., Nakamura,A., Yamauchi,Y., Kawata,S., Miyazaki,A.,
            Nomoto,H., Kameda,H., Terauchi,Y. and Atsumi,T.
  TITLE     Effects of glucokinase haploinsufficiency on the pancreatic
            beta-cell mass and function of long-term high-fat, high-sucrose
            diet-fed mice
  JOURNAL   J Diabetes Investig 15 (12), 1732-1742 (2024)
   PUBMED   39305123
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Zavalin,K., Hassan,A., Zhang,Y., Khera,Z. and Lagrange,A.H.
  TITLE     Region and layer-specific expression of GABAA receptor isoforms and
            KCC2 in developing cortex
  JOURNAL   Front Cell Neurosci 18, 1390742 (2024)
   PUBMED   38894703
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4123)
  AUTHORS   Macpherson,T., Dixon,C.I., Robertson,J., Sindarto,M.M., Janak,P.H.,
            Belelli,D., Lambert,J.J., Stephens,D.N. and King,S.L.
  TITLE     alpha4-Containing GABAA Receptors on DRD2 Neurons of the Nucleus
            Accumbens Mediate Instrumental Responding for Conditioned
            Reinforcers and Its Potentiation by Cocaine
  JOURNAL   eNeuro 10 (8) (2023)
   PUBMED   37553242
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4123)
  AUTHORS   Cattaneo,P., Hayes,M.G.B., Baumgarten,N., Hecker,D., Peruzzo,S.,
            Aslan,G.S., Kunderfranco,P., Larcher,V., Zhang,L., Contu,R.,
            Fonseca,G., Spinozzi,S., Chen,J., Condorelli,G., Dimmeler,S.,
            Schulz,M.H., Heinz,S., Guimaraes-Camboa,N. and Evans,S.M.
  TITLE     DOT1L regulates chamber-specific transcriptional networks during
            cardiogenesis and mediates postnatal cell cycle withdrawal
  JOURNAL   Nat Commun 13 (1), 7444 (2022)
   PUBMED   36460641
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4123)
  AUTHORS   Sente,A., Desai,R., Naydenova,K., Malinauskas,T., Jounaidi,Y.,
            Miehling,J., Zhou,X., Masiulis,S., Hardwick,S.W., Chirgadze,D.Y.,
            Miller,K.W. and Aricescu,A.R.
  TITLE     Differential assembly diversifies GABAA receptor structures and
            signalling
  JOURNAL   Nature 604 (7904), 190-194 (2022)
   PUBMED   35355020
REFERENCE   6  (bases 1 to 4123)
  AUTHORS   Chang,C.C., Luntz-Leybman,V., Evans,J.E., Rotter,A. and
            Frostholm,A.
  TITLE     Developmental changes in the expression of gamma-aminobutyric
            acidA/benzodiazepine receptor subunit mRNAs in the murine inferior
            olivary complex
  JOURNAL   J Comp Neurol 356 (4), 615-628 (1995)
   PUBMED   7560270
REFERENCE   7  (bases 1 to 4123)
  AUTHORS   Watanabe,H., Kimata,K., Line,S., Strong,D., Gao,L.Y., Kozak,C.A.
            and Yamada,Y.
  TITLE     Mouse cartilage matrix deficiency (cmd) caused by a 7 bp deletion
            in the aggrecan gene
  JOURNAL   Nat Genet 7 (2), 154-157 (1994)
   PUBMED   7920633
REFERENCE   8  (bases 1 to 4123)
  AUTHORS   Danciger,M., Farber,D.B. and Kozak,C.A.
  TITLE     Genetic mapping of three GABAA receptor-subunit genes in the mouse
  JOURNAL   Genomics 16 (2), 361-365 (1993)
   PUBMED   8390964
REFERENCE   9  (bases 1 to 4123)
  AUTHORS   Wisden,W., Herb,A., Wieland,H., Keinanen,K., Luddens,H. and
            Seeburg,P.H.
  TITLE     Cloning, pharmacological characteristics and expression pattern of
            the rat GABAA receptor alpha 4 subunit
  JOURNAL   FEBS Lett 289 (2), 227-230 (1991)
   PUBMED   1655526
REFERENCE   10 (bases 1 to 4123)
  AUTHORS   Ymer,S., Draguhn,A., Kohler,M., Schofield,P.R. and Seeburg,P.H.
  TITLE     Sequence and expression of a novel GABAA receptor alpha subunit
  JOURNAL   FEBS Lett 258 (1), 119-122 (1989)
   PUBMED   2556293
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK013727.1 and AC114826.6.
            
            On Jan 28, 2006 this sequence version replaced NM_010251.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK013727.1, AK141571.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1875              AK013727.1         2-1876
            1876-4123           AC114826.6         660-2907            c
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 38.08 cM"
     gene            1..4123
                     /gene="Gabra4"
                     /gene_synonym="Gabra-4"
                     /note="gamma-aminobutyric acid type A receptor subunit
                     alpha 4"
                     /db_xref="GeneID:14397"
                     /db_xref="MGI:MGI:95616"
     exon            1..585
                     /gene="Gabra4"
                     /gene_synonym="Gabra-4"
                     /inference="alignment:Splign:2.1.0"
     CDS             500..2158
                     /gene="Gabra4"
                     /gene_synonym="Gabra-4"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; gamma-aminobutyric acid receptor subunit
                     alpha-4; GABA(A) receptor subunit alpha-4;
                     gamma-aminobutyric acid (GABA) A receptor, subunit alpha
                     4; GABAAR subunit alpha-4"
                     /codon_start=1
                     /product="gamma-aminobutyric acid receptor subunit alpha-4
                     isoform 2 precursor"
                     /protein_id="NP_034381.1"
                     /db_xref="CCDS:CCDS39107.1"
                     /db_xref="GeneID:14397"
                     /db_xref="MGI:MGI:95616"
                     /translation="MVSVQKVPAIALCSGVSLALLHFLCLAACLNESPGQNSKDEKLC
                     PENFTRILDSLLDGYDNRLRPGFGGPVTEVKTDIYVTSFGPVSDVEMEYTMDVFFRQT
                     WIDKRLKYDGPIEILRLNNMMVTKVWTPDTFFRNGKKSVSHNMTAPNKLFRIMRNGTI
                     LYTMRLTISAECPMRLVDFPMDGHACPLKFGSYAYPKSEMIYTWTKGPEKSVEVPKES
                     SSLVQYDLIGQTVSSETIKSITGEYIVMTVYFHLRRKMGYFMIQTYIPCIMTVILSQV
                     SFWINKESVPARTVFGITTVLTMTTLSISARHSLPKVSYATAMDWFIAVCFAFVFSAL
                     IEFAAVNYFTNIQMQKAKKKISKPPPEVPAAPVLKEKHTETSLQNTHANLNMRKRTNA
                     LVHSESDVKSRTEVGNHSSKTSAVQESSEATPKAHLASSPNPFSRANAAETMSAAARG
                     LSSAASPSPHGTLRPASLGSASTRPAFGSRLGRIKTTVNTTGAAGNVSATPPPPAPPP
                     SGSGTSKIDKYARILFPVTFGAFNMVYWVVYLSKDTMEKSESLM"
     exon            586..704
                     /gene="Gabra4"
                     /gene_synonym="Gabra-4"
                     /inference="alignment:Splign:2.1.0"
     exon            705..772
                     /gene="Gabra4"
                     /gene_synonym="Gabra-4"
                     /inference="alignment:Splign:2.1.0"
     exon            773..993
                     /gene="Gabra4"
                     /gene_synonym="Gabra-4"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1076
                     /gene="Gabra4"
                     /gene_synonym="Gabra-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1220
                     /gene="Gabra4"
                     /gene_synonym="Gabra-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1373
                     /gene="Gabra4"
                     /gene_synonym="Gabra-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1633
                     /gene="Gabra4"
                     /gene_synonym="Gabra-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..4123
                     /gene="Gabra4"
                     /gene_synonym="Gabra-4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4069..4074
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gabra4"
                     /gene_synonym="Gabra-4"
                     /note="hexamer: AATAAA"
     regulatory      4086..4091
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gabra4"
                     /gene_synonym="Gabra-4"
                     /note="hexamer: CATAAA"
     polyA_site      4092
                     /gene="Gabra4"
                     /gene_synonym="Gabra-4"
                     /note="major polyA site"
     polyA_site      4108
                     /gene="Gabra4"
                     /gene_synonym="Gabra-4"
ORIGIN      
        1 attgtttcca gccaaaccct ggtaaaggga gggagaaaag cagaaagaag agaagtcagc
       61 ccaggccagt ggcaggggac ccaattgggg ttttgaagtc gctgacccgg agcagggacc
      121 aaggaccgtg ggtggcgtcc cctgagttac aggttggaac aaacttgcct agcttcgagt
      181 ggcaggagct cccaagttca ccggtgagca gcacttgcaa gcttgacaag gtgcggagct
      241 gagcgccggg ctctcgctcg cactgggcgc gagcggcgag ggagggggcg ggcgcgcagg
      301 tcccgcctcc cctggccgcg tgcacacaca cgcccaccgc ggctaggctg gaggagcgcg
      361 ggcgagtgtg agcgcgggcg agtgtgagcg cgagtgtgcg cacgccgcgg gagccactct
      421 gccctctcct cgcaccctgc ccagggcatc tcgagaggct ggaaacgtga acaggcttaa
      481 agtatggcat gttgcgaaga tggtttctgt ccagaaggta cccgcgattg cgctgtgctc
      541 cggggtcagc ctcgccctcc tgcacttcct gtgcctggcg gcttgtttaa acgaatcccc
      601 aggacagaac tcaaaggacg agaaattgtg cccggaaaat tttacccgta ttctggacag
      661 tttgctggat ggttatgaca acaggctgcg tcctggattt gggggtcctg ttacagaagt
      721 gaaaactgat atatatgtca ccagctttgg gcccgtttct gatgttgaaa tggaatacac
      781 tatggatgtg ttcttcagac agacatggat tgacaaaaga ctaaaatatg acggcccaat
      841 tgaaatcttg aggctgaata atatgatggt caccaaagtt tggacccctg atactttctt
      901 caggaatgga aagaaatctg tctcacataa catgacagct ccaaataagc tttttagaat
      961 tatgagaaat ggcactattt tatacacaat gagactcacc ataagtgcgg agtgccccat
     1021 gagactggtg gattttccta tggatggtca tgcctgccct ttgaaatttg ggagttatgc
     1081 atatcccaaa agtgagatga tctacacctg gaccaaaggc cctgagaagt cagtggaggt
     1141 gccaaaggag tcttctagct tagttcaata tgacctcatt gggcagactg tatcaagcga
     1201 gactatcaaa tctattacag gtgaatacat tgttatgacg gtttacttcc acctcagacg
     1261 gaagatgggc tactttatga ttcagacgta tatcccatgc atcatgacag tgattctttc
     1321 tcaagtttcc ttctggataa acaaggagtc tgttccagct agaactgtat ttggaataac
     1381 cacagtcctc acgatgacca ccctaagcat cagtgctcgg cattctttgc ccaaagtgtc
     1441 ctatgcgact gccatggatt ggttcatagc tgtctgtttt gcttttgtat tttcggctct
     1501 tattgagttt gctgctgtca actatttcac caacattcaa atgcagaaag ccaaaaagaa
     1561 gatatcaaag cctcccccag aagttccagc tgctcctgtg ctgaaggaga aacacacaga
     1621 aacatccctt cagaatacac atgccaattt gaacatgagg aaaagaacaa atgccttggt
     1681 ccattcagaa tcggatgtca aaagcagaac tgaggtggga aatcactcca gcaagaccag
     1741 cgctgtccag gagtcttctg aagccacgcc taaggctcac ttagcttcca gtccaaatcc
     1801 attcagcagg gcaaatgcag ctgagactat gtctgctgca gccagaggtc tttcatctgc
     1861 agcatccccc tctcctcatg gcacattgcg gccagcttct ttggggtcag cttccactcg
     1921 ccctgcattt ggatctagac ttgggcgaat taagacaaca gttaatacaa caggggctgc
     1981 tgggaatgtg tcagccacac ctcctccccc tgctccaccg ccttctggat ctggcacaag
     2041 taaaatagac aaatatgctc gtattctctt tccagtcaca tttggagcat ttaacatggt
     2101 ctactgggtt gtttatttat ctaaggacac catggagaaa tcagaaagtc taatgtaatt
     2161 ttgttgctat agtaatttca taacaacgat ggaaatacag actgtctttt aaaatgtttt
     2221 taaaggtaag tattctttac taaaataaaa attctatgta attttttcac ttaaaaaaat
     2281 acaagtcaga tatggagaga gttaattaac tcttcagtgg ctagaaagtg aaccatcttt
     2341 ctttatagaa agatagcttt aaaagaatct attcagtgtt caaattggat agaacacaga
     2401 gcattatgga aagttggagc aagagaaata gagctaggag agatatgtgg tacacccttt
     2461 tgcattgaaa tttgaaaaaa aaaagaaaga ctgtgaattt tttaaatata cccccatgaa
     2521 atcaaactgt cacccaaaac ttcccattgg cactgccctt aaccagaact gtgtgctgga
     2581 agtcatatgc aatgaccttt gcagtttttc atagtacctg caaaataaga cgttctctat
     2641 taaagaaaac actaggaaga ggaaaaagga acaaaacaaa gactgatcaa tacagtgaag
     2701 cctctgctat atcaatacag atgccgacca gggcaaatgt gttttctgac acataattgg
     2761 tcaaacaata cttaacagtt gacttgaaat gttgtgctct gagccaaagt taaattcatt
     2821 gtataaattc ttttttccta gtaagtcatt cagttatgta ctgtatacat ttaccacata
     2881 gttcctcaca gcctggaacc tactgtgttc taggtgatct ttagagaatc taccaaggaa
     2941 gctctctcgc ctttacaaag atatttcaat agtaggacag ccaatgcatg ttaaagaacc
     3001 ataatttagt caaggacatc atgttcttta atgaaggcaa tttagttaga gtcagacaac
     3061 atagccaaca tatttgtttc ttctgagctg acagagggaa ataaataaag ttcctatagc
     3121 agcttggaga taaatacacg ttagtacaat ctagatgatt ggttctcaaa ctataaggaa
     3181 atactgatac aggttgttct gtaggtgaaa aagcattata gacctgaaac atcacaatct
     3241 gggccctgga gagcctaaca gatgacctgc gtcaaatttg atacaggtca cataacacag
     3301 tttgcccact ctaccagaat catccaaata aaagtctgca caggcctttt gtctgcttat
     3361 cccagtagag agcaatatta gctgctgaaa tgctaatgtc ctcgcaagat tctgtgctca
     3421 taacatctgt atgtctggca cgttttgtat ttgtttttct gagagtttga taagatttta
     3481 taaccaagct actttaaaaa ttaaaggagc aactagagaa agaagagaat aaatgtattg
     3541 tagccttgga tggaatgtca gtgagtgtta tgcaatgtat ctgggttctc aggtattgac
     3601 ttttcctttt catcatggag gcttaaccct aattgaatct ttgcagcttt taaaatagct
     3661 caatcttggt tgatattttt gatgtttcac ttccagattt tttttctgta actctacttg
     3721 ttcttagtct gtgtaaagga atgtgttctg ggtttgtcga ggggaaacat ttgatagaaa
     3781 tgtccatctt atagacagaa tctcttattt tctgaagaca cttatattga ttaatatgaa
     3841 cttttgagtt actacattga cttgtgatgg ttgcccatga gatttataaa atttgaaaat
     3901 gtcaaatttt aattgacctc cacctgagaa ttccttcttt ttttatttct aaatatttca
     3961 tagaattaaa tccactttaa agttgaaaga tgttgaagat atctttactt ataaattgat
     4021 tctagaaaac tggggaccgg gaaaatctgt aaatatgttt atacatttaa taaagagtac
     4081 ttgtccataa aagcatataa taaaattatt tcatgagtgt gaa
//
