LOCUS       NM_010248               6008 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus growth factor receptor bound protein 2-associated
            protein 2 (Gab2), transcript variant 1, mRNA.
ACCESSION   NM_010248
VERSION     NM_010248.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Gao,X., Long,R., Qin,M., Zhu,W., Wei,L., Dong,P., Chen,J., Luo,J.
            and Feng,J.
  TITLE     Gab2 promotes the growth of colorectal cancer by regulating the M2
            polarization of tumor-associated macrophages
  JOURNAL   Int J Mol Med 53 (1) (2024)
   PUBMED   37937666
  REMARK    GeneRIF: Gab2 promotes the growth of colorectal cancer by
            regulating the M2 polarization of tumor-associated macrophages.
REFERENCE   3  (bases 1 to 6008)
  AUTHORS   Mo,H., Yang,S. and Chen,A.M.
  TITLE     Inhibition of GAB2 expression has a protective effect on
            osteoarthritis:An in vitro and in vivo study
  JOURNAL   Biochem Biophys Res Commun 626, 229-235 (2022)
   PUBMED   36007472
  REMARK    GeneRIF: Inhibition of GAB2 expression has a protective effect on
            osteoarthritis:An in vitro and in vivo study.
REFERENCE   4  (bases 1 to 6008)
  AUTHORS   Kondreddy,V., Keshava,S., Das,K., Magisetty,J., Rao,L.V.M. and
            Pendurthi,U.R.
  TITLE     The Gab2-MALT1 axis regulates thromboinflammation and deep vein
            thrombosis
  JOURNAL   Blood 140 (13), 1549-1564 (2022)
   PUBMED   35895897
  REMARK    GeneRIF: The Gab2-MALT1 axis regulates thromboinflammation and deep
            vein thrombosis.
REFERENCE   5  (bases 1 to 6008)
  AUTHORS   Chen,S., Cheng,J., Zhong,Y., Liu,R., Lu,Z. and Luo,X.
  TITLE     Liver-specific overexpression of Gab2 accelerates hepatocellular
            carcinoma progression by activating immunosuppression of
            myeloid-derived suppressor cells
  JOURNAL   Oncogene 41 (24), 3316-3327 (2022)
   PUBMED   35411035
  REMARK    GeneRIF: Liver-specific overexpression of Gab2 accelerates
            hepatocellular carcinoma progression by activating
            immunosuppression of myeloid-derived suppressor cells.
            Erratum:[Oncogene. 2022 Jun;41(24):3381. doi:
            10.1038/s41388-022-02337-3.. PMID: 35523947]
REFERENCE   6  (bases 1 to 6008)
  AUTHORS   Dorsey,J.F., Cunnick,J.M., Mane,S.M. and Wu,J.
  TITLE     Regulation of the Erk2-Elk1 signaling pathway and megakaryocytic
            differentiation of Bcr-Abl(+) K562 leukemic cells by Gab2
  JOURNAL   Blood 99 (4), 1388-1397 (2002)
   PUBMED   11830491
  REMARK    GeneRIF: Transfected Gab2 is a limiting signaling component for Erk
            MAP kinase activation and terminal differentiation of K562 CML
            cells.
REFERENCE   7  (bases 1 to 6008)
  AUTHORS   Gu,H., Saito,K., Klaman,L.D., Shen,J., Fleming,T., Wang,Y.,
            Pratt,J.C., Lin,G., Lim,B., Kinet,J.P. and Neel,B.G.
  TITLE     Essential role for Gab2 in the allergic response
  JOURNAL   Nature 412 (6843), 186-190 (2001)
   PUBMED   11449275
REFERENCE   8  (bases 1 to 6008)
  AUTHORS   Yamada,K., Nishida,K., Hibi,M., Hirano,T. and Matsuda,Y.
  TITLE     Comparative FISH mapping of Gab1 and Gab2 genes in human, mouse and
            rat
  JOURNAL   Cytogenet Cell Genet 94 (1-2), 39-42 (2001)
   PUBMED   11701952
  REMARK    GeneRIF: comparative FISH mapping of Gab1 and Gab2 genes in human,
            mouse and rat
REFERENCE   9  (bases 1 to 6008)
  AUTHORS   Nishida,K., Yoshida,Y., Itoh,M., Fukada,T., Ohtani,T.,
            Shirogane,T., Atsumi,T., Takahashi-Tezuka,M., Ishihara,K., Hibi,M.
            and Hirano,T.
  TITLE     Gab-family adapter proteins act downstream of cytokine and growth
            factor receptors and T- and B-cell antigen receptors
  JOURNAL   Blood 93 (6), 1809-1816 (1999)
   PUBMED   10068651
REFERENCE   10 (bases 1 to 6008)
  AUTHORS   Gu,H., Pratt,J.C., Burakoff,S.J. and Neel,B.G.
  TITLE     Cloning of p97/Gab2, the major SHP2-binding protein in
            hematopoietic cells, reveals a novel pathway for cytokine-induced
            gene activation
  JOURNAL   Mol Cell 2 (6), 729-740 (1998)
   PUBMED   9885561
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC074047.6 and AC155933.3.
            
            On Jul 2, 2009 this sequence version replaced NM_010248.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF104244.1, BC103525.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-156               AC074047.6         33948-34103         c
            157-457             AC155933.3         81256-81556
            458-698             AC155933.3         129585-129825
            699-1273            AC155933.3         156611-157185
            1274-1344           AC155933.3         158082-158152
            1345-1609           AC155933.3         159154-159418
            1610-1700           AC155933.3         160152-160242
            1701-1806           AC155933.3         160807-160912
            1807-1932           AC155933.3         161933-162058
            1933-6008           AC155933.3         162659-166734
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 53.39 cM"
     gene            1..6008
                     /gene="Gab2"
                     /gene_synonym="D130058I17Rik; p97"
                     /note="growth factor receptor bound protein 2-associated
                     protein 2"
                     /db_xref="GeneID:14389"
                     /db_xref="MGI:MGI:1333854"
     exon            1..156
                     /gene="Gab2"
                     /gene_synonym="D130058I17Rik; p97"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..2076
                     /gene="Gab2"
                     /gene_synonym="D130058I17Rik; p97"
                     /note="isoform 1 is encoded by transcript variant 1;
                     GRB2-associated-binding protein 2; Grb2 associated binder
                     2; GRB2-associated binder 2; PH domain-containing adaptor
                     molecule p97"
                     /codon_start=1
                     /product="GRB2-associated-binding protein 2 isoform 1"
                     /protein_id="NP_034378.2"
                     /db_xref="CCDS:CCDS21455.1"
                     /db_xref="GeneID:14389"
                     /db_xref="MGI:MGI:1333854"
                     /translation="MSGGGGDDVVCTGWLRKSPPEKKLRRYAWKKRWFILRSGRMSGD
                     PDVLEYYKNEHSKKPLRIINLNLCEQVDAGLTFNKKELQDSFVFDIKTSERTFYLVAE
                     TEADMNKWVQSICQICGFNQAEESTDSLRNLSSASHGPRSSPAEFSSSQHLLRERKSS
                     APSHSSQPTLFTFEPPVSSHMQPTLSTSAPQEYLYLHQCISRRTENARSASFSQGTRQ
                     KSDTAVQKLAQSNGHCINGVGGQVHGFYSLPKPSRHNTEFKDSTYDLPRSLASHGHTK
                     SSLTGSETDNEDVYTFKMPSNTLCRELGDLLVDNMDVPTTPLSAYQIPRTFTLDKNHN
                     AMTVATPGDSAIAPPPRPPKPSQAETPQWGSIQQRPPISENSRSVAATIPRRNTLPAM
                     DNSRLHRASSCETYEYPARGSGESASWSAEPPGKTAVGRSNSASSDDNYVPMNPGSST
                     LLAMERPGDNSQSVYIPMSPGPHHFDPLGYPSTALPIHRGPSRGSEIQPPPVNRNLKP
                     DRKAKPTPLDLRNNTVIDELPFKSPVTKSWSRINSHTFNSSSSQYCRPISTQSITSTD
                     SGDSEENYVPMQNPVSASPVPSGTNSPAPKKSTGSVDYLALDFQPGSPSPHRKPSTSS
                     VTSDEKVDYVQVDKEKTQALQNTMQEWTDVRQSSEPSKGAKL"
     exon            157..457
                     /gene="Gab2"
                     /gene_synonym="D130058I17Rik; p97"
                     /inference="alignment:Splign:2.1.0"
     exon            458..698
                     /gene="Gab2"
                     /gene_synonym="D130058I17Rik; p97"
                     /inference="alignment:Splign:2.1.0"
     exon            699..1273
                     /gene="Gab2"
                     /gene_synonym="D130058I17Rik; p97"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1344
                     /gene="Gab2"
                     /gene_synonym="D130058I17Rik; p97"
                     /inference="alignment:Splign:2.1.0"
     exon            1345..1609
                     /gene="Gab2"
                     /gene_synonym="D130058I17Rik; p97"
                     /inference="alignment:Splign:2.1.0"
     exon            1610..1700
                     /gene="Gab2"
                     /gene_synonym="D130058I17Rik; p97"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..1806
                     /gene="Gab2"
                     /gene_synonym="D130058I17Rik; p97"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..1932
                     /gene="Gab2"
                     /gene_synonym="D130058I17Rik; p97"
                     /inference="alignment:Splign:2.1.0"
     exon            1933..6008
                     /gene="Gab2"
                     /gene_synonym="D130058I17Rik; p97"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5294..5299
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gab2"
                     /gene_synonym="D130058I17Rik; p97"
                     /note="hexamer: AATAAA"
     polyA_site      5316
                     /gene="Gab2"
                     /gene_synonym="D130058I17Rik; p97"
                     /note="major polyA site"
     regulatory      5982..5987
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gab2"
                     /gene_synonym="D130058I17Rik; p97"
                     /note="hexamer: AATAAA"
     polyA_site      6003
                     /gene="Gab2"
                     /gene_synonym="D130058I17Rik; p97"
ORIGIN      
        1 gccggctgac ccagcagccc gcgcccgcgc ccttgtccgc gcgagccggc ggcgggctcc
       61 agtttagccg gcgacatgag cggcggcggc ggcgacgacg tggtgtgtac cggctggctg
      121 aggaaatcgc ctcccgagaa gaagttgagg cgctatgcct ggaagaaacg ctggtttata
      181 cttcggagtg gccgaatgag tggagatcca gatgttctgg aatactacaa gaatgagcac
      241 tccaagaaac ccctgcggat catcaacctg aacttgtgtg agcaggtgga tgcaggcctg
      301 accttcaaca agaaagagct gcaggatagt tttgtgtttg atatcaagac cagcgagcgc
      361 acattttacc tggtggctga gacagaggct gacatgaata agtgggtcca gagcatctgc
      421 cagatctgcg gcttcaatca ggctgaagag agcacagact ccctgaggaa cctttcttca
      481 gccagtcatg gtccccgctc ttctccagct gagttcagct ccagtcagca cctgctccga
      541 gaacggaagt cctcagcccc ttcacactct agccagccta ctttattcac gtttgagccc
      601 cctgtgtcaa gccacatgca gcctaccttg tccaccagtg cacctcagga gtatctctac
      661 ttgcaccagt gcataagcag aaggacagaa aatgcaagga gtgccagctt ctctcagggc
      721 acccggcaga agagtgatac agctgtgcaa aaacttgccc agagcaatgg acactgtatc
      781 aacggcgtcg gaggtcaagt ccatggcttc tatagccttc ccaagccaag ccgacacaat
      841 acagaattca aagacagtac ttatgatctc ccacggagcc tggcttccca tggccacacc
      901 aagagcagcc tcacagggtc tgagactgat aacgaggatg tgtacacctt caagatgccc
      961 agcaacaccc tgtgtcggga acttggagac ctccttgtgg acaatatgga tgtcccaacc
     1021 actcctctct cagcctacca gatccctaga actttcacac tggacaagaa ccacaatgcc
     1081 atgacagtgg ccactcctgg agattcagcc atagctcccc caccccggcc acccaagcca
     1141 agtcaggcag aaacacctca atggggcagc attcagcaaa gacctccaat cagcgaaaat
     1201 agcagatctg tagctgctac tatccccagg cgcaataccc tccctgcaat ggacaacagc
     1261 cgactccatc gagcttcttc ctgtgagacc tacgagtacc cggcacgagg cagtggggaa
     1321 agtgccagct ggtctgctga acctccagga aagactgccg taggtcgatc aaatagtgcc
     1381 agctctgatg acaactacgt gcccatgaac ccaggttctt ctaccctgct ggctatggaa
     1441 cgaccagggg acaactccca gagtgtctac atccccatga gcccaggacc ccatcacttt
     1501 gacccacttg gctacccgtc cacagccctt cctattcaca gaggccccag ccgaggaagt
     1561 gaaatccagc cacccccggt caaccgaaac ctcaagcctg acagaaaagc aaagccaaca
     1621 ccccttgacc tgagaaacaa cactgtcatc gatgagctgc ccttcaagtc acctgtcacc
     1681 aagtcttggt ccaggatcaa cagccacacc tttaactcca gttcctccca gtactgccgt
     1741 cctatctcca cacagagcat caccagcaca gactcgggag acagtgagga gaactatgtc
     1801 cctatgcaaa acccagtatc tgcatcccct gttcccagtg gcactaacag cccagctcca
     1861 aagaagagta ctggcagtgt ggattatctc gccctggact tccagccggg ctccccaagc
     1921 cctcaccgca agccatccac atcatctgtc acatcagatg agaaggtaga ctatgtccaa
     1981 gtggataaag agaagaccca ggccctgcag aacaccatgc aggagtggac agatgtgcgg
     2041 cagtcctccg aaccttccaa gggtgccaag ctgtaatgaa gggggccacc aagttcaaga
     2101 gccatgaggc agctgcccag acctgcctct tctgtcacct ctttctccat ttcttcctac
     2161 acctctctcc cctgtaccac tttcagcctt caaagcactc aacatcaggg acctgaccct
     2221 ttcccatcag aggcaagggc ctggtggaga cacttcctct gccacttggg ttccacctat
     2281 gacttctgtc aatactagct gtgcctccac caccttagtt aggagctgtt gccaaaaaag
     2341 cttcctgctt tggacccatt tgtctgctgc ttggactctt agagggaggg ctgaagctct
     2401 gagctaggct catgcctcgt gtctgctcac tgctctctac cctctacctc agggctgctt
     2461 ctccatcccc agcggagaat gataacccag ggagccaaga accagccgac caaagtacac
     2521 atgtgttttc ctatccctgc aactttggca aggaaaaggc aaggtgacac aagatgaaat
     2581 ggttgatagt ggaagtcgga agttgccttg agtgagaaag aaagtatgtc cccagatatg
     2641 ggagtgtagc tgataacctg cagggtggta cccaatatca ggccatggtg gttgctgaag
     2701 actggaatat gaaggactgc tggaaggaaa actgacaatg ataagcaacc aggaacagca
     2761 gaagtctttg gggtgacaaa gcttggagct agaggactct gccactctgg gacaggcagt
     2821 cctcttccct ggccctgata atgagatgaa acagagtagg gaagacagta gcaggtgata
     2881 tgggggatgg gaggcaggaa ggatcctggg acaggagtgt gctaagctca gagcagtaca
     2941 gggacactgc catggaaaga aagtttctag taggtaggag ggctgactta agagactggg
     3001 aggccggcaa gacatgtagc aagtggggac atgtgtccaa aaggcctgat ggggagccca
     3061 aggccacatg cagaagtaca gcaggaagca gaatgagaag gagtcatact ttgaccaagg
     3121 aaatctagaa atcacatgag ggtctgggta atggcttgtg ccagacagaa gcacctctga
     3181 cctaatgcca gaccagatct tttctctgtt gtcctcagct gcagggatgg tagaaactaa
     3241 tcctctcctt ccatggcttc agaggtcacc agagcagaag ggatgggaag ctgtcattgc
     3301 ctaaggctct taaggtaggg cagggagatc tgaatgtgtt tgtgttgaaa gttgttgacc
     3361 aaattttcaa gcatttcatt gcagaagaaa aacagggaaa accagcattt cattttgccc
     3421 tagatgaaca gattgttgtc ctctaaaaac taggactgtt gagaactaaa gacacacctc
     3481 acctctctcc tccagagagc cctgtctagg agttcctcag tagtagcccc tcttctcaca
     3541 ttatgatgga tcatttagca atgcttgcca aaccctgtgc taccttgtat agtcatacct
     3601 tgtccctaac tacaggtcac aatcagtgag aaaagtaaac cataaagatg actggcaagt
     3661 acatagaaag gaaaagtgct aaaggaaact gagctacagg gaggggaagc ctcaaggagg
     3721 gagcagaatg agttttaggc aaaggatagg cagaggtggg tccatggagg agacagacga
     3781 ggtgagggaa gcctcggctt tgggaacaga gggtctttga aggtatgaag tgtgcactgt
     3841 caccttgcgt gaaatggggc tatgaatggt tgcactgagc agtattacaa ggtacttggc
     3901 tggctctgcc cagactcatg gtgtccttta aggccttcag tggatctccc atgtggcaaa
     3961 gcttttgact gatgctgccg gagctgatag cagggctagg tatcattggc tttagcagcc
     4021 accccataga ccccagagtg cattttctga atgagtgtgc catgtgaatg gagctggctg
     4081 ccatcttcca ggattgaggt attattctag ttagtctcaa ggcctccgtc ttgctctggt
     4141 tcacctgtgg aaagctgagc tttggctcat aatcccagac tgctagccat gactaatgta
     4201 cccaaccatc tacccatcct ccaagcccaa ataggaaaag ccaattccat ttaggaaaaa
     4261 aaaatatcca taagtcaaag ttcacctccc ctggtggctg tcaaggacta agagccaaga
     4321 acatccatca cctcttagca tagctcccac aagtacctgt cctgcagggc agggcctctt
     4381 cccacttcat cctactagac taaactttag taggaacagt aaagaaagga gatggcatct
     4441 ccttgagact tgccagtctg tgaaatggga tggtcacagc tgatactctg ctgctgtaag
     4501 aacctggatg atcatttgaa agtctgagtt actggataag agcggcacac agaacattag
     4561 cttattaact gcctccctct ctgtctggat tttgcggata gctacattga ctccatgaaa
     4621 agaagccaca ttcccagaac agaagcacaa cccttctgaa gttactggaa tgagggtaca
     4681 ggtctgcctt tgggcccttt atataacagt agctatagtc ccagccgtgg ctccctcccc
     4741 tccctgcaac acagctctgt aagctatgag aagagggagc tcttccttcc cattcaggcc
     4801 tttggctgta aagtgtgggg aagcaggctg accttagagg gccatattgc tggggcttcc
     4861 catatctggg ttcttggaaa ggcaggaaca tccagacttg ccatgggcac ctgctgataa
     4921 tttgtgctgt cctggtatct ggtggtgtgt gttcttgtgt aatctgtggg ctacttgtgt
     4981 cttgaaatgt agaatcattt cattcaggat tgttactatt tgttgggaag aaaaaatgaa
     5041 atagaagcaa cctagtctta catcgtgtct tgttaccatt taagattgac atttaatttt
     5101 tcaagtcatt gttggtgcct aatcacttaa gttattaatt tattctgttt tcaaaaaatt
     5161 gtaacatatt tatcttgtga agtctggggg tggggtggga aatgtgttca agtgagtcct
     5221 gtttcacagt ggtaacaaat ggtacaggcc tggagcttgc aggtcccttt ttattatggt
     5281 gtagaagcag gacaataaag tcctttagaa atgtaaccca gagtagactg ctggagagtc
     5341 tttagggggt ggaaacttac tcttgtccca tcctgggcag tcttaggact gatgctgtcc
     5401 tatagatacc ttctctgaca aactcatttc aggatcattt catgaataac cccaggagct
     5461 ggtgtgccca attctgctca tcttgatggg ctgccattgg gcacttttcc aggccaggag
     5521 aagaatgaac cttctttact ccgaccaaca tctggcatct gcagtggtgc acacctgtaa
     5581 tcccagcact ctagaagctg aagcagggac aggacagcca gtttccttga cagttccaga
     5641 gtctggcatt tggaccaaaa cctctacccc ttgtcacagc accattttga ccttccagat
     5701 actttgttct taagagaatg atcgtgggtc caggccagtc ccagaccctc cctcaatata
     5761 cagccaccca tgtccctctt cagctgcaag gctgtgacta tgaacagaaa gagctggatg
     5821 ttgggtctag agagatgacc gcgctgtttg gttcccagca cccacactgg gtggctcaca
     5881 acagcttgta atccagttcc acgggatttg atgccctcta ctggcctcca tacatacatg
     5941 gtacacacac acttaacaga gatacacatt catacaataa aaataaaagt tgtttttaaa
     6001 aaagccct
//
