LOCUS       NM_033648                554 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus FXYD domain-containing ion transport regulator 4
            (Fxyd4), transcript variant 1, mRNA.
ACCESSION   NM_033648
VERSION     NM_033648.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Tang,T., Li,L., Tang,J., Li,Y., Lin,W.Y., Martin,F., Grant,D.,
            Solloway,M., Parker,L., Ye,W., Forrest,W., Ghilardi,N., Oravecz,T.,
            Platt,K.A., Rice,D.S., Hansen,G.M., Abuin,A., Eberhart,D.E.,
            Godowski,P., Holt,K.H., Peterson,A., Zambrowicz,B.P. and de
            Sauvage,F.J.
  TITLE     A mouse knockout library for secreted and transmembrane proteins
  JOURNAL   Nat Biotechnol 28 (7), 749-755 (2010)
   PUBMED   20562862
REFERENCE   2  (bases 1 to 554)
  AUTHORS   Geering,K.
  TITLE     FXYD proteins: new regulators of Na-K-ATPase
  JOURNAL   Am J Physiol Renal Physiol 290 (2), F241-F250 (2006)
   PUBMED   16403837
  REMARK    Review article
REFERENCE   3  (bases 1 to 554)
  AUTHORS   Crambert,G., Li,C., Claeys,D. and Geering,K.
  TITLE     FXYD3 (Mat-8), a new regulator of Na,K-ATPase
  JOURNAL   Mol Biol Cell 16 (5), 2363-2371 (2005)
   PUBMED   15743908
REFERENCE   4  (bases 1 to 554)
  AUTHORS   Goldschmidt,I., Grahammer,F., Warth,R., Schulz-Baldes,A., Garty,H.,
            Greger,R. and Bleich,M.
  TITLE     Kidney and colon electrolyte transport in CHIF knockout mice
  JOURNAL   Cell Physiol Biochem 14 (1-2), 113-120 (2004)
   PUBMED   14976412
  REMARK    GeneRIF: Corticosteroid hormone induced factor (CHIF) is a small
            epithelial-specific protein regulated by aldosterone and K+ intake.
REFERENCE   5  (bases 1 to 554)
  AUTHORS   Aizman,R., Asher,C., Fuzesi,M., Latter,H., Lonai,P., Karlish,S.J.
            and Garty,H.
  TITLE     Generation and phenotypic analysis of CHIF knockout mice
  JOURNAL   Am J Physiol Renal Physiol 283 (3), F569-F577 (2002)
   PUBMED   12167609
  REMARK    GeneRIF: data are consistent with an effect of CHIF on the
            Na(+)-K(+)-ATPase specific to the outer and inner medullary duct
REFERENCE   6  (bases 1 to 554)
  AUTHORS   Sweadner,K.J. and Rael,E.
  TITLE     The FXYD gene family of small ion transport regulators or channels:
            cDNA sequence, protein signature sequence, and expression
  JOURNAL   Genomics 68 (1), 41-56 (2000)
   PUBMED   10950925
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK018728.1 and BC086918.1.
            
            Summary: This gene encodes a member of a family of small membrane
            proteins that share a 35-amino acid signature sequence domain,
            beginning with the sequence PFXYD and containing 7 invariant and 6
            highly conserved amino acids. The approved human gene nomenclature
            for the family is FXYD-domain containing ion transport regulator.
            Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2,
            also known as the gamma subunit of the Na,K-ATPase, regulates the
            properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma),
            FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to
            induce channel activity in experimental expression systems.
            Transmembrane topology has been established for two family members
            (FXYD1 and FXYD2), with the N-terminus extracellular and the
            C-terminus on the cytoplasmic side of the membrane. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CK790002.1, AI267073.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-88                AK018728.1         1-88
            89-554              BC086918.1         350-815
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 55.7 cM"
     gene            1..554
                     /gene="Fxyd4"
                     /gene_synonym="0610008I02Rik; Chif"
                     /note="FXYD domain-containing ion transport regulator 4"
                     /db_xref="GeneID:108017"
                     /db_xref="MGI:MGI:1889005"
     exon            1..45
                     /gene="Fxyd4"
                     /gene_synonym="0610008I02Rik; Chif"
                     /inference="alignment:Splign:2.1.0"
     exon            46..88
                     /gene="Fxyd4"
                     /gene_synonym="0610008I02Rik; Chif"
                     /inference="alignment:Splign:2.1.0"
     exon            89..157
                     /gene="Fxyd4"
                     /gene_synonym="0610008I02Rik; Chif"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..384
                     /gene="Fxyd4"
                     /gene_synonym="0610008I02Rik; Chif"
                     /note="channel inducing factor"
                     /codon_start=1
                     /product="FXYD domain-containing ion transport regulator 4
                     precursor"
                     /protein_id="NP_387468.1"
                     /db_xref="CCDS:CCDS20468.1"
                     /db_xref="GeneID:108017"
                     /db_xref="MGI:MGI:1889005"
                     /translation="MEEITCAFLLLLAGLPALEASDPVDKDSPFYYDWESLQLGGLIF
                     GGLLCIAGIAMALSGKCKCRRTHKPSSLPGKATPLIIPGSANTC"
     exon            158..190
                     /gene="Fxyd4"
                     /gene_synonym="0610008I02Rik; Chif"
                     /inference="alignment:Splign:2.1.0"
     exon            191..214
                     /gene="Fxyd4"
                     /gene_synonym="0610008I02Rik; Chif"
                     /inference="alignment:Splign:2.1.0"
     exon            215..289
                     /gene="Fxyd4"
                     /gene_synonym="0610008I02Rik; Chif"
                     /inference="alignment:Splign:2.1.0"
     exon            290..326
                     /gene="Fxyd4"
                     /gene_synonym="0610008I02Rik; Chif"
                     /inference="alignment:Splign:2.1.0"
     exon            327..364
                     /gene="Fxyd4"
                     /gene_synonym="0610008I02Rik; Chif"
                     /inference="alignment:Splign:2.1.0"
     exon            365..554
                     /gene="Fxyd4"
                     /gene_synonym="0610008I02Rik; Chif"
                     /inference="alignment:Splign:2.1.0"
     regulatory      533..538
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fxyd4"
                     /gene_synonym="0610008I02Rik; Chif"
                     /note="hexamer: ATTAAA"
     polyA_site      554
                     /gene="Fxyd4"
                     /gene_synonym="0610008I02Rik; Chif"
                     /note="major polyA site"
ORIGIN      
        1 agacttcctc tctgcaccac tcccctggct cgagtcatct cccgggacac tgaaacacag
       61 agatattacc tgctaagtca cacacctgca gcctgcagtc tcaccctggg tcctgatatg
      121 gaggaaatca cctgtgcctt tctcctgctg ctagcaggtc tgccggcctt ggaagccagt
      181 gacccagttg ataaagacag tcccttctac tatgactggg agagcctgca gctgggagga
      241 ttgatttttg gagggctcct gtgcatcgct ggaattgcca tggccctgag tggcaagtgc
      301 aaatgtaggc gtacccataa gcccagttcc ttacctggaa aagccactcc actcatcatt
      361 ccaggctctg ccaatacctg ctgaactgaa cacaggacca agtttggagg caggtttttg
      421 acaactttct gccgtacttc tcctctggag accttcctct ccaggatggc ttccctagaa
      481 catactgtta agtcttcatt gacaggaaag ggtgtggcaa agctgatttt atattaaact
      541 ggtcttgctg ctca
//
