LOCUS       NM_001301373            2575 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus follistatin (Fst), transcript variant 1, mRNA.
ACCESSION   NM_001301373 XM_006517527
VERSION     NM_001301373.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Kay,R.G.G., Reeves,R., Siggers,P., Greenaway,S., Mallon,A.M.,
            Wells,S., Koo,B.K., Mayere,C., Nef,S., Greenfield,A. and Simon,M.M.
  TITLE     Gonadal sex reversal at single-cell resolution in Znrf3-deficient
            mice
  JOURNAL   Development 151 (23) (2024)
   PUBMED   39629665
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Koo,H.Y., Oh,J.H., Duran Alonso,M.B., Hernandez,I.L.,
            Gonzalez-Vallinas,M., Alonso,M.T., Tena,J.J., Gil-Galvez,A.,
            Giraldez,F., Bok,J. and Schimmang,T.
  TITLE     Analysis of Meis2 knockout mice reveals Sonic hedgehog-mediated
            patterning of the cochlear duct
  JOURNAL   Dev Dyn (2024) In press
   PUBMED   39351969
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   3  (bases 1 to 2575)
  AUTHORS   Zhu,S., Huo,S., Wang,Z., Huang,C., Li,C., Song,H., Yang,X., He,R.,
            Ding,C., Qiu,M. and Zhu,X.J.
  TITLE     Follistatin controls the number of murine teeth by limiting
            TGF-beta signaling
  JOURNAL   iScience 27 (9), 110785 (2024)
   PUBMED   39286503
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2575)
  AUTHORS   Souali-Crespo,S., Condrea,D., Vernet,N., Feret,B., Klopfenstein,M.,
            Grandgirard,E., Alunni,V., Cerciat,M., Jung,M., Mayere,C., Nef,S.,
            Mark,M., Chalmel,F. and Ghyselinck,N.B.
  TITLE     Loss of NR5A1 in mouse Sertoli cells after sex determination
            changes cellular identity and induces cell death by anoikis
  JOURNAL   Development 150 (24) (2023)
   PUBMED   38078651
REFERENCE   5  (bases 1 to 2575)
  AUTHORS   Kimura,F., Sidis,Y., Bonomi,L., Xia,Y. and Schneyer,A.
  TITLE     The follistatin-288 isoform alone is sufficient for survival but
            not for normal fertility in mice
  JOURNAL   Endocrinology 151 (3), 1310-1319 (2010)
   PUBMED   20032047
  REMARK    GeneRIF: The FST288-only isoform is sufficient for development.
            Loss of FST303 and FST315 isoforms results in fertility defects
            that resemble activin hyperactivity and premature ovarian failure.
REFERENCE   6  (bases 1 to 2575)
  AUTHORS   Lin,S.Y., Craythorn,R.G., O'Connor,A.E., Matzuk,M.M., Girling,J.E.,
            Morrison,J.R. and de Kretser,D.M.
  TITLE     Female infertility and disrupted angiogenesis are actions of
            specific follistatin isoforms
  JOURNAL   Mol Endocrinol 22 (2), 415-429 (2008)
   PUBMED   17932109
REFERENCE   7  (bases 1 to 2575)
  AUTHORS   Ritvos,O., Tuuri,T., Eramaa,M., Sainio,K., Hilden,K., Saxen,L. and
            Gilbert,S.F.
  TITLE     Activin disrupts epithelial branching morphogenesis in developing
            glandular organs of the mouse
  JOURNAL   Mech Dev 50 (2-3), 229-245 (1995)
   PUBMED   7619733
REFERENCE   8  (bases 1 to 2575)
  AUTHORS   Albano,R.M., Arkell,R., Beddington,R.S. and Smith,J.C.
  TITLE     Expression of inhibin subunits and follistatin during
            postimplantation mouse development: decidual expression of activin
            and expression of follistatin in primitive streak, somites and
            hindbrain
  JOURNAL   Development 120 (4), 803-813 (1994)
   PUBMED   7600958
REFERENCE   9  (bases 1 to 2575)
  AUTHORS   van den Eijnden-van Raaij,A.J., Feijen,A., Lawson,K.A. and
            Mummery,C.L.
  TITLE     Differential expression of inhibin subunits and follistatin, but
            not of activin receptor type II, during early murine embryonic
            development
  JOURNAL   Dev Biol 154 (2), 356-365 (1992)
   PUBMED   1330789
REFERENCE   10 (bases 1 to 2575)
  AUTHORS   Nakamura,T., Takio,K., Eto,Y., Shibai,H., Titani,K. and Sugino,H.
  TITLE     Activin-binding protein from rat ovary is follistatin
  JOURNAL   Science 247 (4944), 836-838 (1990)
   PUBMED   2106159
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK079916.1, BC144926.1,
            Z29532.1, AC110817.6, AW987053.1 and BX514305.1.
            
            On Aug 16, 2014 this sequence version replaced XM_006517527.1.
            
            Summary: The protein encoded by this gene binds to and negatively
            regulates activin, as well as other members of the transforming
            growth factor beta family, and acts to prevent uncontrolled
            cellular proliferation. This protein also contains a
            heparin-binding sequence. It is expressed in many of the tissues in
            which activin is synthesized and is thought to clear activin from
            the circulation by attachment to the cell surface. Alternative
            splicing results in multiple transcript variants that encode
            multiple protein isoforms, including FST315 and FST288, that differ
            at their C-terminus. Another isoform, FST303 is thought to be
            produced by proteolytic cleavage of FST315. These isoforms differ
            in their localization and in their ability to bind heparin. While
            FST315 is a circulating protein, FST288 is tissue-bound, and FST303
            is gonad-specific. While deletion of all isoforms results in
            embryonic lethality, expression of just FST288 is sufficient for
            embryonic development, but the resultant mice have fertility
            defects. [provided by RefSeq, Aug 2014].
            
            Transcript Variant: This variant (1) encodes the longest isoform,
            FST315 (PMID:17932109).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC144926.1, X83377.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-176               AK079916.1         2-177
            177-1448            BC144926.1         1-1272
            1449-1482           Z29532.1           1081-1114
            1483-1539           AC110817.6         131472-131528
            1540-2059           AW987053.1         1-520
            2060-2093           AC110817.6         132049-132082
            2094-2575           BX514305.1         1-482
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 64.15 cM"
     gene            1..2575
                     /gene="Fst"
                     /gene_synonym="FS"
                     /note="follistatin"
                     /db_xref="GeneID:14313"
                     /db_xref="MGI:MGI:95586"
     exon            1..450
                     /gene="Fst"
                     /gene_synonym="FS"
                     /inference="alignment:Splign:2.1.0"
     CDS             366..1400
                     /gene="Fst"
                     /gene_synonym="FS"
                     /note="isoform FST315 precursor is encoded by transcript
                     variant 1; activin-binding protein"
                     /codon_start=1
                     /product="follistatin isoform FST315 precursor"
                     /protein_id="NP_001288302.1"
                     /db_xref="CCDS:CCDS88534.1"
                     /db_xref="GeneID:14313"
                     /db_xref="MGI:MGI:95586"
                     /translation="MVCARHQPGGLCLLLLLLCQFMEDRSAQAGNCWLRQAKNGRCQV
                     LYKTELSKEECCSTGRLSTSWTEEDVNDNTLFKWMIFNGGAPNCIPCKETCENVDCGP
                     GKKCRMNKKNKPRCVCAPDCSNITWKGPVCGLDGKTYRNECALLKARCKEQPELEVQY
                     QGKCKKTCRDVFCPGSSTCVVDQTNNAYCVTCNRICPEPSSSEQYLCGNDGVTYSSAC
                     HLRKATCLLGRSIGLAYEGKCIKAKSCEDIQCGGGKKCLWDSKVGRGRCSLCDELCPD
                     SKSDEPVCASDNATYASECAMKEAACSSGVLLEVKHSGSCNSISEETEEEEEEEDQDY
                     SFPISSILEW"
     exon            451..642
                     /gene="Fst"
                     /gene_synonym="FS"
                     /inference="alignment:Splign:2.1.0"
     exon            643..861
                     /gene="Fst"
                     /gene_synonym="FS"
                     /inference="alignment:Splign:2.1.0"
     exon            862..1086
                     /gene="Fst"
                     /gene_synonym="FS"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1317
                     /gene="Fst"
                     /gene_synonym="FS"
                     /inference="alignment:Splign:2.1.0"
     exon            1318..2561
                     /gene="Fst"
                     /gene_synonym="FS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2538..2543
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fst"
                     /gene_synonym="FS"
                     /note="hexamer: AATAAA"
     polyA_site      2561
                     /gene="Fst"
                     /gene_synonym="FS"
                     /note="major polyA site"
ORIGIN      
        1 gctttgattt cgggcacctc cgacagataa ttgggaaggg tttccagaag gtgggaaatg
       61 tcacctgatt cacactgaac ttttaaaagc tccccacccc caaagagccg gcacaccctc
      121 ggtcgcggcc gccctcccac agccccacac actgggagac cgcccaccgc aaacctcgga
      181 gacccccgtc tagatttaaa gcgcggctgc gcccggcttc tgacgtccat tgaatcgcgc
      241 gggcggccgg tggcgagcgc ggggctgcgc cgggatcgct gcgccctccg ccgctcgcct
      301 ctgcgacgcg cgccgctcgc ccgagccacc cgccgccgcg ctcctcgccc cgcgcctgcc
      361 ccaggatggt ctgcgccagg caccagcccg gcgggctctg cctcctgctg ctgctactct
      421 gccagttcat ggaggaccgc agcgcccagg ctgggaattg ctggctccgc caagcaaaga
      481 acggccgctg ccaggtcctg tataagacag aactgagcaa ggaagagtgt tgcagcaccg
      541 gccggctgag cacctcatgg accgaggagg atgtgaacga caatactctc ttcaagtgga
      601 tgattttcaa cgggggcgcc cccaactgca tcccttgtaa agaaacgtgt gagaacgtgg
      661 actgcggacc tgggaaaaaa tgtcgaatga acaagaagaa taaaccccgc tgcgtctgtg
      721 ccccagactg ttccaacatc acctggaagg gcccagtgtg tgggctggat gggaaaacct
      781 accgcaacga atgtgcactc ctcaaggcca gatgcaaaga gcagccggaa ctagaagtac
      841 agtaccaggg caaatgtaaa aagacttgta gggatgtttt ttgtccaggc agctccactt
      901 gtgtggtgga tcagaccaat aatgcctact gtgtgacctg taatcggatt tgcccagagc
      961 cctcctcttc tgaacagtac ctttgtggaa atgatggagt gacttactcc agcgcctgcc
     1021 acctgagaaa ggccacctgc ttgctgggca gatccattgg attagcctat gagggaaagt
     1081 gtatcaaagc aaagtcctgt gaagatatcc agtgtggcgg cgggaaaaaa tgcctatggg
     1141 attccaaggt tggcagaggt cgctgctctc tctgcgatga gctgtgtcct gacagtaagt
     1201 cggatgagcc ggtctgtgcc agtgacaatg ccacatacgc cagcgagtgt gccatgaagg
     1261 aagctgcctg ctcttctggc gtgcttcttg aagtgaagca ttctggatct tgcaactcca
     1321 tctcggaaga aacggaggaa gaggaggagg aggaagacca ggactacagc tttcctatct
     1381 cttccattct agagtggtaa actctccacc aatgttcagt gttgacatag cctttgggca
     1441 aaacgaaaca caacaaaata aataataata ataaataata aaggaaagaa aaaagataga
     1501 aaagaagata agaaaaagaa aaatatattg ttcataatgt aaataagtgt atgcttattt
     1561 atttgggggg aaaactatac attaaaggac ctttgtccta agctctctcc caggccacgt
     1621 tgttattcac tgggcatgga gagatggtca tttcgtggtc tactggatga ggcctatagt
     1681 tgagacttgt agacatttat ttatactgtg tcatgtttta taatttatac ataaaatgtc
     1741 tggttgactg tacaccttgt ttttttgaag aaatttattc gtgaaaggaa gagcagttgt
     1801 tatttattgt gaggtctctt gcttgtaaag taaaactgtt ttttttttcc cccttgtaaa
     1861 ccatttaagt ccattcctca ctatgcacac ccacctgtct ctctccgcat ctcactgtta
     1921 gattcttttc caccttttta acaaatttgc atgtcagttt ctgtcatgtt tatttattgg
     1981 attctcagct gcctaatctg tacatacctg atccctcggg ttttgtttac aaggaatctt
     2041 gactgaccaa aaggcattgt aactctgcca cagtacaagg tacagagtat gggcatctta
     2101 gaggaaatgt ctgcttccgt cccctcgttc tggtgaacgt tgggagagag gatgagagag
     2161 acattaaaat tctggagatg tgcttttatg atgttacaga tccaaagaca tagagtgatg
     2221 tgggtgtcag gagactgaag agaggtgaaa ggcacacttt caacctgtca ttcagcgttt
     2281 cttttgagct tagtcagctg taccttttca attagttctt tttcaaccaa tgtgtcacta
     2341 aaagttccat caaagcttta tcagttcaag tttcttgctt ttcataatac ttttttctga
     2401 tgcaatttta tattttcaaa catggcaagt taaatataaa ttcatttaaa tatatagttt
     2461 tgtacttttc taccatgtaa atgtgcaatg tatataaaag ttataatgtg tatttgtaga
     2521 taaatgatga gtgaaaaaat aaaaaaaaaa tgtaaaagcc aaaaaaaaaa aaaaa
//
