LOCUS       NM_028127               4674 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus FERM domain containing 6 (Frmd6), transcript variant
            2, mRNA.
ACCESSION   NM_028127 NM_176984
VERSION     NM_028127.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Lyu,C., Lyu,G.W., Mulder,J., Uhlen,M., Cai,X.H., Hokfelt,T. and
            Sten Shi,T.J.
  TITLE     Expression and regulation of FRMD6 in mouse DRG neurons and spinal
            cord after nerve injury
  JOURNAL   Sci Rep 10 (1), 1880 (2020)
   PUBMED   32024965
  REMARK    GeneRIF: Expression and regulation of FRMD6 in mouse DRG neurons
            and spinal cord after nerve injury.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4674)
  AUTHORS   Zhang,Z., Wei,S., Du,H., Su,Z., Wen,Y., Shang,Z., Song,X., Xu,Z.,
            You,Y. and Yang,Z.
  TITLE     Zfhx3 is required for the differentiation of late born D1-type
            medium spiny neurons
  JOURNAL   Exp Neurol 322, 113055 (2019)
   PUBMED   31491374
REFERENCE   4  (bases 1 to 4674)
  AUTHORS   Wang,Z., Chen,Y., Chen,X., Zheng,X., Xu,G., Yuan,Z., Zhao,H.,
            Chen,W., Li,L., Zheng,N., Shen,X., Li,Y., Qi,X. and Cai,D.
  TITLE     The TrkB-T1 receptor mediates BDNF-induced migration of aged
            cardiac microvascular endothelial cells by recruiting Willin
  JOURNAL   Aging Cell 18 (2), e12881 (2019)
   PUBMED   30667167
  REMARK    GeneRIF: The aged CMECs utilize BDNF-TrkB-T1 signalling to recruit
            Willin as a downstream effector to further activate the Hippo
            pathway, which then promotes migration.
REFERENCE   5  (bases 1 to 4674)
  AUTHORS   Moleirinho,S., Patrick,C., Tilston-Lunel,A.M., Higginson,J.R.,
            Angus,L., Antkowiak,M., Barnett,S.C., Prystowsky,M.B.,
            Reynolds,P.A. and Gunn-Moore,F.J.
  TITLE     Willin, an upstream component of the hippo signaling pathway,
            orchestrates mammalian peripheral nerve fibroblasts
  JOURNAL   PLoS One 8 (4), e60028 (2013)
   PUBMED   23593160
  REMARK    GeneRIF: Willin is predominantly expressed in fibroblasts and that
            Willin expression activates the Hippo signaling cascade
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4674)
  AUTHORS   Ishiuchi,T. and Takeichi,M.
  TITLE     Nectins localize Willin to cell-cell junctions
  JOURNAL   Genes Cells 17 (5), 387-397 (2012)
   PUBMED   22512338
  REMARK    GeneRIF: results suggest that the nectin-afadin interaction plays a
            role in the correct localization of Willin
REFERENCE   7  (bases 1 to 4674)
  AUTHORS   Ishiuchi,T. and Takeichi,M.
  TITLE     Willin and Par3 cooperatively regulate epithelial apical
            constriction through aPKC-mediated ROCK phosphorylation
  JOURNAL   Nat Cell Biol 13 (7), 860-866 (2011)
   PUBMED   21685893
  REMARK    GeneRIF: Report a willin(FRMD6)/Par3-aPKC-ROCK pathway that
            controls epithelial apical morphology.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 4674)
  AUTHORS   Narumiya,H., Hidaka,K., Shirai,M., Terami,H., Aburatani,H. and
            Morisaki,T.
  TITLE     Endocardiogenesis in embryoid bodies: novel markers identified by
            gene expression profiling
  JOURNAL   Biochem Biophys Res Commun 357 (4), 896-902 (2007)
   PUBMED   17462595
REFERENCE   9  (bases 1 to 4674)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC140360.4 and AC132325.4.
            
            On Mar 17, 2023 this sequence version replaced NM_028127.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC053929.1, SRR13948564.1301195.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849379
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-71                AC140360.4         68871-68941         c
            72-337              AC140360.4         30302-30567         c
            338-428             AC140360.4         21863-21953         c
            429-532             AC140360.4         17181-17284         c
            533-609             AC140360.4         15951-16027         c
            610-796             AC140360.4         13639-13825         c
            797-952             AC140360.4         10623-10778         c
            953-1018            AC140360.4         7355-7420           c
            1019-1087           AC140360.4         6567-6635           c
            1088-1262           AC140360.4         4338-4512           c
            1263-1598           AC140360.4         502-837             c
            1599-1730           AC132325.4         17141-17272
            1731-1822           AC132325.4         19606-19697
            1823-4674           AC132325.4         21648-24499
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 29.37 cM"
     gene            1..4674
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /note="FERM domain containing 6"
                     /db_xref="GeneID:319710"
                     /db_xref="MGI:MGI:2442579"
     exon            1..71
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /inference="alignment:Splign:2.1.0"
     exon            72..337
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /inference="alignment:Splign:2.1.0"
     CDS             239..2107
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /note="isoform 2 is encoded by transcript variant 2; FERM
                     domain-containing protein 6"
                     /codon_start=1
                     /product="FERM domain-containing protein 6 isoform 2"
                     /protein_id="NP_082403.2"
                     /db_xref="CCDS:CCDS25959.1"
                     /db_xref="GeneID:319710"
                     /db_xref="MGI:MGI:2442579"
                     /translation="MNKLTFHNNKAMQDRRRVCIFLPNDKSVSIIINVKILCHQLLVQ
                     VCDLLRLKDSHLFGLSVIQNNEHVYMELSQKLYKYCPKEWKKEASKVRQYEVTWGIDQ
                     FGPPMIIHFRVQYYVENGKLISDRIARYYYYWHLRKQVLHSQCVLREEAYFLLAAFAL
                     QADLGNFKRKLHHGDYFEPEAYFPAWVVSKRGKDYILKHIPNMHKDQFALTASEAYLK
                     YIKEAVRLDDVAIHYYRLYKDKREAEGSLTLGLTMRGIQIFQNLEEEKQLLYDFPWTN
                     VGKLVFVGKKFEILPDGLPSARKLVYYTGCPTRSRHLLQLLSNSHRLYMNLQPVLRHL
                     RKQEENEEKKQYRESYISDNLDLDMDPLEKRSRASGSSAGSVKHKRLSRHSTASHSSS
                     HTSGIEADTKPRDPGPEDSCSGSAMHRKLKTCSSMTSHGSSHTSGVESGGKDRLEEDS
                     QDEEIEMLVDDPRDLEPMPEESLEVSPEMCIYITEDMLLSRKLNGHSGLIVKEIGSST
                     SSSSETVVRLRGQSTDSLPQTICRKPKTSTDRHSLSLDDIRLYQKDFLRIAGLCQDTA
                     QSYTFGCGHELDESGLYCNSCLAQQCVNIQDAFPVKRASKYFSLDLTHDEVPEFVV"
     exon            338..428
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /inference="alignment:Splign:2.1.0"
     exon            429..532
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /inference="alignment:Splign:2.1.0"
     exon            533..609
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /inference="alignment:Splign:2.1.0"
     exon            610..796
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /inference="alignment:Splign:2.1.0"
     exon            797..952
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1018
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1087
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1262
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..1598
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /inference="alignment:Splign:2.1.0"
     exon            1599..1730
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /inference="alignment:Splign:2.1.0"
     exon            1731..1822
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /inference="alignment:Splign:2.1.0"
     exon            1823..4674
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2209
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /note="major polyA site"
     polyA_site      2449
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
     polyA_site      3821
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
     regulatory      4648..4653
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /note="hexamer: AATAAA"
     polyA_site      4674
                     /gene="Frmd6"
                     /gene_synonym="2610019M19Rik; 4930488L10Rik; Willin"
                     /note="major polyA site"
ORIGIN      
        1 gcgagtaggc tcggcgccgc tggagcagac gccggttgtg agtcggaagc ctggcgctgt
       61 cgactttgga ggtctcccag tggactttct acagctatga aacctacact gtggggcacc
      121 acgatgcttc cgttggccgt atgatgagga ggagaagttg gctttggagt gctgggagcc
      181 tgagaagttg ctgagcaccc agcgccccca gcccgcccac tggcccgccc ataacacaat
      241 gaacaaactg accttccata acaacaaagc catgcaggac cgtcgcagag tgtgtatttt
      301 cctccccaat gacaagtccg tgagcatcat cataaatgtt aaaattctgt gtcaccagtt
      361 gctggtccag gtgtgtgacc tgctcaggtt aaaggacagt cacctctttg gtctcagtgt
      421 tatacaaaat aatgaacatg tatatatgga attgtcacaa aagctttata agtattgtcc
      481 aaaagaatgg aaaaaggagg ccagcaaggt acggcaatac gaagtcactt ggggcatcga
      541 ccagtttggg ccccccatga tcatccactt ccgggtgcag tactacgtgg agaatgggaa
      601 gctgatcagt gaccgaattg caagatacta ttattactgg cacctacgga aacaggtgct
      661 gcactcccag tgtgtgctca gagaggaggc ctacttcctg ctggcagcct ttgcactgca
      721 agctgacctc ggcaacttca aaaggaaact gcaccacgga gactactttg agccagaggc
      781 ttacttcccg gcatgggttg tttccaagcg ggggaaggac tacatcctga aacacatccc
      841 aaacatgcac aaggaccagt ttgccctgac ggcctccgag gcctacctaa agtacatcaa
      901 agaagccgtc cgactggacg acgtcgccat ccattactac agactgtaca aggataaaag
      961 ggaggctgaa ggctcactga ccctaggact gaccatgcga ggaatacaga tttttcagaa
     1021 tctagaagaa gagaaacaat tgctctatga tttcccctgg acaaatgttg ggaagttggt
     1081 gtttgtgggc aagaagtttg agattttgcc agatggcctt ccctccgcca ggaagctggt
     1141 ctactacaca gggtgtccca cgcgctcccg gcatctcctg cagctcctga gcaacagcca
     1201 ccgcctctac atgaacctgc agcccgtcct gcgccacctc cgcaagcagg aggagaatga
     1261 agagaaaaag cagtaccggg aatcctacat cagcgacaac ctggaccttg acatggaccc
     1321 gctggaaaag cggtcccgag ccagtgggag cagcgctggc agcgtgaagc ataagcgcct
     1381 gtcccgccac tccacggcca gccacagcag ctcccacacc tccggcatcg aggcagacac
     1441 caagccccgg gacccagggc cggaagacag ctgttcaggc agcgccatgc accggaagct
     1501 gaagacctgc agctccatga ccagccacgg cagctcccac acctctgggg ttgagagtgg
     1561 aggcaaagac cgcctggaag aggactcgca agatgaggaa atcgagatgc tggtggatga
     1621 ccccagggac ctggagccga tgcctgaaga gtcgctggaa gtcagcccag agatgtgtat
     1681 ctacatcacg gaagatatgc tcctgtcgag gaagctgaac ggacactcag ggttaattgt
     1741 gaaagaaatc ggctcctcca cctccagctc ttcggaaacg gttgtcaggc tgcgtggaca
     1801 gagcaccgac tcccttccac agacgatatg tcgaaaacca aagacttcca ccgatcgcca
     1861 tagcctgagc cttgacgaca tcagactgta ccagaaagac ttcctgcgca tcgcgggcct
     1921 gtgtcaggac actgctcaga gctacacgtt tgggtgtggc catgaactgg atgagagcgg
     1981 tctctactgc aacagctgcc tggctcagca gtgtgtcaac atacaggacg cattcccagt
     2041 gaaaagagcc agcaagtact tttctctgga ccttactcac gacgaagtcc cagagttcgt
     2101 cgtctgagtc gcccctgcgg gcagccctgc gggcagccgc tgtctgctgg aggctgtgga
     2161 gtctgagggt ctttacacat tatttgtgcc ataacttttt caccccaaac ttagcttttt
     2221 ctttatagta ttcgagatgg aaacaaaagc cttgggacag ttgcacttta agtattatgc
     2281 agaggtaaaa gaaacagaga atgtaagagg aagacaagtg cccagattgt ctattgcccc
     2341 tttggaagga agtgtgcttt ccagctttac ccagatttca gactgtcaga ctgcagtgtg
     2401 tttgttcatc tcttgtgttc cggttcaaat ttatgtttat cacgtgaaag atgttatagg
     2461 cttatctgtt tgcttatggg tttttcagcc acttcctcat gaggcacggt ttgaggggga
     2521 ggacaaaggg gagttctctc ttcttttctg gagagccatt cttagtacat agccattgtt
     2581 gcctcacaga ggttggtccg aaatgaacac tgaagttggt gggagtctta tgttctagcc
     2641 aaagatgtgt atgtgatgga agatggaagc cccactcaga gtccagaagg cgtgtgccgg
     2701 gacactgggc tccttcacaa atggacagtg ctctgtccgc tgttttgttc ttccatgacc
     2761 ttcccaggaa gctctgggat tggcctgtgc ttgtgacatg gtacattttg cagtttacaa
     2821 agctggtgta tgttaacaac cattgggaat gtctcagaag acatttgttt ggttgtattt
     2881 cctttgttgg tttttaatgg tggccaacag atggtgatgc tagtttctgc tttaacaaga
     2941 tacccttact gtatgtatat gttatacgta tctagatacg tgggactctg tgtgtttatg
     3001 tgtttgaagc ttagtgactc ttcatttgga cttcagtata tgtcttgcac acatagaatt
     3061 tcttgtttaa tgtcaaggat gcttagacac agacaatcag ttgataaatg tagagttaag
     3121 agtgactttt ccatgtgtgc ctttggccaa gattctcaac cctgattgca taatagactt
     3181 gtccagagag cttttaaaac atgcccgggt cccaccaggc cagtttaaac cagtctcctg
     3241 tggctgggag attgatttaa agtgtaccag gttgagacag taatgcacag taggattgga
     3301 gatccaagag tgtctttcta ttcattttga agtaaaagta tacagaaaat attagtgata
     3361 gagaatgggc cacttttaag acagggcctg ctactgttac agtgtataat gaagccagga
     3421 gaatgagttt ggtcaacttg atatccattg aagacgttgc tcgcccttca ggagagaact
     3481 tcatagcaca atgtctttct aggagatgtt tttaatgatt tagtatttta caacatttgt
     3541 ttaccatatt ttgatactcc atttttgcta tctgccaggt tttattaaaa agaagactat
     3601 gtattatttt ctaaagaaac tcatattttt gtacaaactt atgtttccaa gtaaggaaga
     3661 aatagatgta gggtcaggca gaacatagca gtgtttcccc ctggctaggt cagtgtccca
     3721 gagcccgtgg tggccacaga catgcccggt caccatgctg ccctcctgtg ctggcacggc
     3781 ctcagcaggg tcacatccag taactctcac ctgacactaa agaaggaaaa agaagttctg
     3841 tgctcatgac tttattttgc ttttgttgaa tgcttttaga agaaccaaag tttcagatgc
     3901 cagaatgtat aagtgtctca gtgtccacag ataggcaaag ggcaaagggg tccgtcacct
     3961 gatggcttct tttgcttccg agtcattgca tagtctcatc attgctcaca gatgcttagg
     4021 ggccacaccc tggtgaagta cgcgatctcc agagcacaga tggccgtgta ttcccacagc
     4081 cctcggcatc tcttgcagat gcagagatgg aaaggttgct tgcttggctg tgtgctttat
     4141 accttttttc tctctgctcc agaatccagt cttcagggtt ctgcccaggc tgtcgatcgc
     4201 catttgccct cttccaggca gacttggaaa tagtcttaaa gggttttcaa agacaagatc
     4261 agccaggaaa cagtttctca tttctgaccc acgggagaat catagacata tatgtatgtg
     4321 gagctccact ttgaagaatt gacattcttg tattgggcag tcgttttcca aagtaaaagg
     4381 aggaaagtat aaagccgtca ctcacgctgc acttgctagc gtgtccatgg ctggcagacg
     4441 caccagtgca tctgtcacct ccctgcgtca cctgcatctg tatttgcaac ggtgagcttt
     4501 gtatctaagt aagctgtaaa cagtcctttc tgtgaaagga tcatcgtgtc gggatgatac
     4561 tgaaagtatg tttaaaaaaa aaaaaaaaag ctacggcgtt attttgtaat tatttcttag
     4621 aggtgtttca tggtatgcgc gaccatcaat aaagttaact tttttgcctt taag
//
