LOCUS       NM_001167898            6626 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Mus musculus Fras1 related extracellular matrix protein 3 (Frem3),
            mRNA.
ACCESSION   NM_001167898 XM_984073
VERSION     NM_001167898.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   Kiyozumi,D., Mori,M., Kodani,M. and Ikawa,M.
  TITLE     Genetic mutation of Frem3 does not causeFraser syndrome in mice
  JOURNAL   Exp Anim 69 (1), 104-109 (2020)
   PUBMED   31554749
  REMARK    GeneRIF: FREM3 mutant homozygotes were born at the expected
            Mendelian ratio and did not possess any defects characteristic of
            Fraser syndrome.
REFERENCE   2  (bases 1 to 6626)
  AUTHORS   Diman,N.Y., Brooks,G., Kruithof,B.P., Elemento,O., Seidman,J.G.,
            Seidman,C.E., Basson,C.T. and Hatcher,C.J.
  TITLE     Tbx5 is required for avian and Mammalian epicardial formation and
            coronary vasculogenesis
  JOURNAL   Circ Res 115 (10), 834-844 (2014)
   PUBMED   25245104
REFERENCE   3  (bases 1 to 6626)
  AUTHORS   Makrygiannis,A.K., Pavlakis,E., Petrou,P., Kalogeraki,E. and
            Chalepakis,G.
  TITLE     Segmental and restricted localization pattern of Fras1 in the
            developing meningeal basement membrane in mouse
  JOURNAL   Histochem Cell Biol 140 (5), 595-601 (2013)
   PUBMED   24101214
REFERENCE   4  (bases 1 to 6626)
  AUTHORS   Kerecuk,L., Long,D.A., Ali,Z., Anders,C., Kolatsi-Joannou,M.,
            Scambler,P.J. and Woolf,A.S.
  TITLE     Expression of Fraser syndrome genes in normal and polycystic murine
            kidneys
  JOURNAL   Pediatr Nephrol 27 (6), 991-998 (2012)
   PUBMED   21993971
  REMARK    GeneRIF: Report Fras1 up-regulation/Frem down-regulation in
            nephrons from mice with polycystic kidney disease and Frem2
            mutations.
REFERENCE   5  (bases 1 to 6626)
  AUTHORS   Yates,L.L., Papakrivopoulou,J., Long,D.A., Goggolidou,P.,
            Connolly,J.O., Woolf,A.S. and Dean,C.H.
  TITLE     The planar cell polarity gene Vangl2 is required for mammalian
            kidney-branching morphogenesis and glomerular maturation
  JOURNAL   Hum Mol Genet 19 (23), 4663-4676 (2010)
   PUBMED   20843830
REFERENCE   6  (bases 1 to 6626)
  AUTHORS   Kiyozumi,D., Sugimoto,N., Nakano,I. and Sekiguchi,K.
  TITLE     Frem3, a member of the 12 CSPG repeats-containing extracellular
            matrix protein family, is a basement membrane protein with tissue
            distribution patterns distinct from those of Fras1, Frem2, and
            QBRICK/Frem1
  JOURNAL   Matrix Biol 26 (6), 456-462 (2007)
   PUBMED   17462874
  REMARK    GeneRIF: Frem3 is a basement membrane protein that is distinct from
            other 12 CSPG repeats-containing extracellular matrix protein
            family proteins in its tissue distribution and competence to
            assemble into the basement membrane.
REFERENCE   7  (bases 1 to 6626)
  AUTHORS   Chiotaki,R., Petrou,P., Giakoumaki,E., Pavlakis,E., Sitaru,C. and
            Chalepakis,G.
  TITLE     Spatiotemporal distribution of Fras1/Frem proteins during mouse
            embryonic development
  JOURNAL   Gene Expr Patterns 7 (4), 381-388 (2007)
   PUBMED   17251066
  REMARK    GeneRIF: Study reports the immunofluorescence pattern of Frem3 and
            a comparative analysis of the spatiotemporal localization of all
            Fras1/Frem proteins during mouse embryonic development.
REFERENCE   8  (bases 1 to 6626)
  AUTHORS   Kiyozumi,D., Osada,A., Sugimoto,N., Weber,C.N., Ono,Y., Imai,T.,
            Okada,A. and Sekiguchi,K.
  TITLE     Identification of a novel cell-adhesive protein spatiotemporally
            expressed in the basement membrane of mouse developing hair
            follicle
  JOURNAL   Exp Cell Res 306 (1), 9-23 (2005)
   PUBMED   15878328
REFERENCE   9  (bases 1 to 6626)
  AUTHORS   Smyth,I., Du,X., Taylor,M.S., Justice,M.J., Beutler,B. and
            Jackson,I.J.
  TITLE     The extracellular matrix gene Frem1 is essential for the normal
            adhesion of the embryonic epidermis
  JOURNAL   Proc Natl Acad Sci U S A 101 (37), 13560-13565 (2004)
   PUBMED   15345741
REFERENCE   10 (bases 1 to 6626)
  AUTHORS   Takamiya,K., Kostourou,V., Adams,S., Jadeja,S., Chalepakis,G.,
            Scambler,P.J., Huganir,R.L. and Adams,R.H.
  TITLE     A direct functional link between the multi-PDZ domain protein GRIP1
            and the Fraser syndrome protein Fras1
  JOURNAL   Nat Genet 36 (2), 172-177 (2004)
   PUBMED   14730302
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC130675.7 and AC132606.3.
            
            On Nov 18, 2009 this sequence version replaced XM_984073.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219383.109847.1,
                                           SRR6116558.193957.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5196              AC130675.7         153022-158217
            5197-5289           AC130675.7         160188-160280
            5290-5436           AC132606.3         192760-192906       c
            5437-5667           AC132606.3         187305-187535       c
            5668-5793           AC132606.3         186947-187072       c
            5794-6045           AC132606.3         169161-169412       c
            6046-6195           AC132606.3         165455-165604       c
            6196-6626           AC132606.3         160719-161149       c
FEATURES             Location/Qualifiers
     source          1..6626
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 38.35 cM"
     gene            1..6626
                     /gene="Frem3"
                     /gene_synonym="Gm795; NV2"
                     /note="Fras1 related extracellular matrix protein 3"
                     /db_xref="GeneID:333315"
                     /db_xref="MGI:MGI:2685641"
     exon            1..5196
                     /gene="Frem3"
                     /gene_synonym="Gm795; NV2"
                     /inference="alignment:Splign:2.1.0"
     CDS             42..6425
                     /gene="Frem3"
                     /gene_synonym="Gm795; NV2"
                     /note="embryonic blastocoelar extracellular matrix
                     protein; FRAS1-related extracellular matrix protein 3; NV
                     domain containing protein NV2; NV domain-containing
                     protein 2"
                     /codon_start=1
                     /product="FRAS1-related extracellular matrix protein 3
                     precursor"
                     /protein_id="NP_001161370.1"
                     /db_xref="CCDS:CCDS52606.1"
                     /db_xref="GeneID:333315"
                     /db_xref="MGI:MGI:2685641"
                     /translation="MAGDSLSLPGMSLQLLVTLTCLLLTCALRERVPLSENVSHCELY
                     LPPWGALNGGSHQNPGILIANSGFQVPQGRSVWLNPLRDLVIRVQRGDQCKVTVLDFP
                     RLQGALSPHQFPCDFGAQQVKYTHFGSPSSTRTRIQLQVRYDAGNSTLVLPFTLQVNV
                     VFPKLQLVARNRPLKVFKVLGCSHAIDRRVLNFASRSRCRLTVLPHPGGPLPKYGLLV
                     DTAGNPLSRGHLADCEAFIQAGVRYQHTTTPSSPSRDYVPMLVELLGPESQGTRSVEV
                     LAQEYFQIQVRIQKRARSTAPIPSLAALMIVEVEQSLLTALTPDVLAAEDSESDPDDL
                     VFNILNVPEAPPGHHGGQGYVVNTDDPLGLPVSFFTQKELRELKIAYRPPTGSSEGDH
                     VFQLKLQVVDEDGDTSEPFAFMVVVKSMNVLAPIASYRGGLVVFEGQARPLSDALSFQ
                     VSDKDNSKEIKIVAVRGLQHGQLVVHGAPAGCKNFTLADLSAGRVVYQHDGSDSYSDN
                     IILKMEGEHHRVDFLFPITIVPVDDAAPVLTANMGLSVTEGQVVQISPFVLCATDIDS
                     EDSAILFVLEDEHLEGKEEETHEDLAPGSYSSSQHPGNMLLRQAEPPSSLLYSDWHYV
                     EKEGLYETVVTEWLQRDIMEGRLFFSHPGPHSPSPVAHLAFHVQDDQDPPNLSNQHFF
                     TISIQPADTSQPQLSPETTLEMTVQGYQLTPFQQKYLQYTDQNSDEQNLWYTLLTRPT
                     DADSNHQVQAGEIVLTDSPGRPIVHFTQTQVNHQKIAYRPPQRNLGIVPRVVQFTYKV
                     EDTAGNSVSGTFTVFLQPLNNQPPKVINRGFAVLEGDSFILGRGELNVSDPDTNDDQI
                     FFILVWGPQHGHLQYFKKYMVPGESFMLADVINGSISYQNSRDQTDSDVIYLEVSDGV
                     HHIPITVQVSVQPTVADRSPGISITGTPVLATSLTVLENAASEITMDVTHGRKNTNDL
                     MLSFIVEDKPKLGMILVNGWPAEQVTQEDLLGGAVSYVHTSGEIGFQRVHDTFSFILS
                     KDAYHRAMRDNILERVLVEVTVLPVDNMAPKVLVRKPFIVYEGGKNLLTPQHVNIEDV
                     DTSKDEILCTLTVQPSSGYLENLAPAPGSEVSRAGNPISAFFVKDVRVGCINYVQSIH
                     KGIEPGADQFTFYCSDGINFSPKVLLPLTILPTNDEQPQLFTREFVVLEGMSRVIDTS
                     LVNGVDADFPSDKLHFRLTVFPQHGQITQQLATGSKPIHSFTLQEIQEPSSIAYEHDD
                     SESTEDSFEVWLSDGRHTTHTTVPIAVILVDDEVPQLTINDGLEIETGHSEIITNHIL
                     KAIDLDSDNKSLSFVLHSEPQQGLLQRLRKPGGDAKHNLTVGMNFTQDEIDRGLIHYI
                     HTGQGEAVDLKFDVTDGVNTLRDRYFYITIDNSNSAVPEIVSKRVTLTEGNRMTLTTE
                     LLNTSDIHSPGEQLLYSITRAPSTGHLESSDQPGEPMASFTQLQLARNKISYVHISND
                     KIKLDHFELRVTGGHHSESRIFRIFVTEQDNKKPTLTIQALALQRGDNIVVTPSQLTV
                     EDEDTPADFILFTITQIPIHGRILYNGSRPVTTFTKKDLTKSLILYCHDGSETSKDSF
                     SFTVTDGIHTGFYVFPDTSLETHVPQTVWIQISPFDDRLPQMGINRGATALKLLHTGH
                     LGFLITNEYLQATHQGVPHRLLTYKVTRGPEHGYIVNAGLGNENTHMFTQADIDEMRV
                     YYILNKGRGRATRDTFYFSVETRGGKQLRNQPFHLNWAWISLEKEYYIVDEDSPFFEV
                     TLTRRGYLGGTSVVSIGTKGDTAEENKDFKGKAPMLVQFSPGQSTATWRVGLIPDTKY
                     ETSETFQIILSEPGAAALEFPEMATVEIVDPEDESTVYIPEAEYQLAEAVGEFLVPVR
                     RSGDTSQELTVICSTRPGSATGTIPSVQLSLSDYVSRPEDNTSMLHFEKDESEKTCQV
                     LIINDSLYEEEESFSIALSLPTGGQLGAKFPTARVTILADREDEPALHFECSEYHAEE
                     SAGYVEVAVWRRGTGLSQASSVIVRSRGTEEQAAEAGTDYIGVRQHLHFSPGVSVQRL
                     RVTILDDLPQPAFEGPEMFELLLQMPTGAVIGEPNKTTVTINDSVTNCEEECV"
     exon            5197..5289
                     /gene="Frem3"
                     /gene_synonym="Gm795; NV2"
                     /inference="alignment:Splign:2.1.0"
     exon            5290..5436
                     /gene="Frem3"
                     /gene_synonym="Gm795; NV2"
                     /inference="alignment:Splign:2.1.0"
     exon            5437..5667
                     /gene="Frem3"
                     /gene_synonym="Gm795; NV2"
                     /inference="alignment:Splign:2.1.0"
     exon            5668..5793
                     /gene="Frem3"
                     /gene_synonym="Gm795; NV2"
                     /inference="alignment:Splign:2.1.0"
     exon            5794..6045
                     /gene="Frem3"
                     /gene_synonym="Gm795; NV2"
                     /inference="alignment:Splign:2.1.0"
     exon            6046..6195
                     /gene="Frem3"
                     /gene_synonym="Gm795; NV2"
                     /inference="alignment:Splign:2.1.0"
     exon            6196..6626
                     /gene="Frem3"
                     /gene_synonym="Gm795; NV2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6601..6606
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Frem3"
                     /gene_synonym="Gm795; NV2"
                     /note="hexamer: AATAAA"
     polyA_site      6621
                     /gene="Frem3"
                     /gene_synonym="Gm795; NV2"
                     /note="major polyA site"
ORIGIN      
        1 ggcatcctct agatatatca actccagaat tccatcggac tatggctgga gattctctga
       61 gccttcctgg gatgtccctg cagctgctag tgactctcac ctgcctgctt ctgacttgtg
      121 cactgaggga aagggtaccc ttgagtgaaa acgtgtccca ctgtgagctt tacctgcctc
      181 cctggggagc gcttaatggt ggtagtcacc agaaccctgg cattctgatc gccaactccg
      241 ggttccaagt gccacagggc cgctcggttt ggcttaaccc actgcgggat ctagtgattc
      301 gagtgcagcg cggggatcag tgcaaggtga cagtgctgga cttcccgcgg ctccaaggcg
      361 cgctctcccc gcaccaattt ccctgtgatt tcggggccca acaggttaag tacactcact
      421 tcggctcgcc cagctccaca agaacccgga tacagctgca ggtgcgctat gacgcaggga
      481 attccacgct ggtgctgccc ttcactctgc aagtgaatgt agtcttcccc aagcttcagt
      541 tggtggcgcg caacaggcca ctgaaagtgt ttaaagtgct aggctgcagc catgccatcg
      601 acaggagagt gctaaacttt gcctccagga gcaggtgcag gctcaccgtg cttcctcacc
      661 ctggcggacc actccccaag tatggactct tggtggacac cgctgggaac cctctctcca
      721 ggggccatct ggccgactgt gaggctttta tccaggctgg cgtacgctac cagcatacca
      781 ccacaccatc ctcacccagc agggactatg tgcccatgct agtggagctg cttggaccag
      841 agtcacaagg tactaggtca gtggaggtgc tggcccagga gtacttccaa attcaagtca
      901 gaatccagaa gcgagccagg agcacagcac caatacctag tctcgcggct ctaatgatag
      961 tggaggttga acagtcctta ctgacagccc tgacacctga tgtgctggct gcagaagaca
     1021 gcgagtcaga ccctgacgac ctagtcttca acattctcaa tgtacccgag gccccacctg
     1081 gacaccatgg tggacagggt tatgtggtga acacagatga ccccctgggc cttccagtct
     1141 ctttctttac ccagaaagag cttcgagagc tgaaaatagc ctatcgaccc ccaacgggga
     1201 gctcagaagg ggaccacgtc tttcagctca aactgcaggt ggtggatgaa gatggtgaca
     1261 cctctgagcc ttttgctttc atggtggtgg taaagtctat gaatgtgctg gctccaatag
     1321 ctagctacag aggaggactt gtggtgtttg aaggccaggc aaggcccttg tctgatgccc
     1381 taagctttca ggtcagtgat aaagataatt ccaaagaaat aaaaattgtt gcagtcagag
     1441 gtttgcaaca cgggcagctg gtagtgcatg gggcacctgc tgggtgcaag aatttcacac
     1501 tggcagacct aagtgcaggg agagtggtgt accagcatga tggcagtgac tcttacagtg
     1561 acaacatcat cctcaagatg gagggtgagc accaccgggt ggacttcctc ttccccatca
     1621 ctatcgtgcc cgtggatgat gcggcaccag tgctcaccgc caacatggga ctctccgtga
     1681 ctgaagggca agtggtccag atatccccct ttgtcttatg tgccaccgat attgattctg
     1741 aggactcagc tatcctcttt gtgttggaag acgaacacct ggaaggaaaa gaggaagaaa
     1801 cacatgaaga tctggcccct ggttcctaca gctcaagcca gcacccaggc aacatgctgc
     1861 tgaggcaggc tgagccccct tcgtcccttc tgtacagtga ctggcactat gtggaaaagg
     1921 agggccttta tgagacggta gtgactgagt ggctgcagag agacataatg gaagggagac
     1981 tgttcttctc ccatcctgga ccacatagcc catctccagt ggctcatctg gccttccatg
     2041 tgcaggatga ccaggaccca ccaaacctct ccaaccagca cttcttcacc atcagcatcc
     2101 agcctgcaga tacgtcgcag cctcagctat ctccagaaac gaccctagaa atgacagtgc
     2161 aaggatacca gctcactccc ttccagcaga agtacctcca gtacactgac cagaactcag
     2221 atgaacagaa cctgtggtac actctactga cgcgaccaac agacgctgac agcaaccatc
     2281 aagtccaggc tggagagatc gtgctcactg attcaccagg tagacccatc gttcatttta
     2341 cccaaaccca ggtcaatcat caaaaaattg cttatcgacc cccacagagg aatcttggca
     2401 ttgtacctcg agttgtacaa ttcacctata aggtggaaga cactgcaggc aacagtgtgt
     2461 caggtacatt cacagtattc ctgcagcctt tgaacaatca gccaccaaaa gtcatcaata
     2521 gaggcttcgc tgtcttggag ggagatagct ttattcttgg cagaggtgaa cttaatgttt
     2581 cagaccctga caccaatgat gatcaaattt tcttcatttt agtttggggt ccccagcatg
     2641 gacacttgca gtactttaaa aaatatatgg ttccaggaga atcatttatg ctagctgatg
     2701 ttatcaatgg gagcatctct tatcagaaca gcagagacca gactgacagt gacgtcatct
     2761 acctagaggt aagtgatgga gtgcaccata tacccatcac tgtccaggtt tctgtgcagc
     2821 ccactgtggc tgacagaagt cctgggattt ctatcacagg gactccagta ttggctactt
     2881 ctctaactgt ccttgaaaat gcagcttctg agatcactat ggatgttact catggcagaa
     2941 agaacacaaa tgatttgatg ctatctttca ttgtggagga caagcctaaa ctgggcatga
     3001 ttctagtgaa tggttggcct gcagagcaag tcacccaaga ggacctcctt ggtggggcag
     3061 tcagctatgt tcacactagt ggtgaaattg gcttccaaag ggtgcatgat actttcagct
     3121 tcatcctctc caaagatgct tatcaccggg ccatgagaga caacatactg gagagggtac
     3181 tggtggaagt aacagtactg cccgtggata acatggcccc caaggtcttg gtaagaaagc
     3241 ccttcatcgt atatgaaggt gggaagaacc tcttgacccc acaacatgtc aacattgaag
     3301 atgtagacac ttccaaagat gaaatccttt gcactctgac tgtacagcca tcctctggct
     3361 acctggaaaa cttggcacca gctcctggtt cagaagtgtc acgggctggc aaccccatca
     3421 gtgccttctt cgtcaaggat gttcgagtgg ggtgcatcaa ttatgtacag agtatccaca
     3481 aaggaataga accaggagca gatcaattca ccttttattg ctctgacggc atcaacttct
     3541 ccccaaaggt cctcctccct ctaaccatct tgcccaccaa tgatgagcag cctcaacttt
     3601 tcacccgtga gtttgtggta ttagagggga tgagccgggt catagacacc tcactggtca
     3661 atggagtaga tgctgacttc ccatcagata agcttcactt ccgactcacg gtcttccccc
     3721 agcatggaca aatcacacag cagctggcca caggcagcaa gcccatccac agctttacct
     3781 tgcaggagat ccaagagccc tcctccatcg cctatgaaca tgatgactca gagagcacgg
     3841 aggacagctt tgaggtctgg ctgagtgatg gcaggcacac cactcacaca acggtgccca
     3901 ttgctgtaat tttggtggat gatgaggttc ctcagttgac catcaatgac ggactggaaa
     3961 tagaaactgg acactctgag atcatcacaa atcatatcct caaagccata gatctcgact
     4021 ctgataacaa gagtctcagt tttgttctcc actctgagcc tcagcaggga cttctgcagc
     4081 ggctgagaaa gccaggagga gatgcaaagc acaacctcac tgtgggaatg aatttcaccc
     4141 aggatgaaat agacagaggc cttatccatt atatccacac aggccaggga gaggctgttg
     4201 atctcaaatt tgatgtgact gatggtgtta acactttgag agatcgctat ttctatatca
     4261 ccattgacaa ctcaaacagt gccgtccctg agatagtcag caaaagagtc accttgacag
     4321 aaggtaacag aatgaccctc accaccgaac tgcttaacac cagtgacatc cacagccctg
     4381 gtgaacaact tctctacagt atcacacgag ctcctagcac aggccacttg gaaagctctg
     4441 accagcctgg ggagcccatg gcttctttta ctcagcttca actggccaga aacaagatat
     4501 cctatgttca tatttcaaat gataaaataa agttggacca ctttgagctt agagtgactg
     4561 gaggacacca ctctgagtcc agaatcttta gaatatttgt cacagagcag gacaacaaga
     4621 aacctacctt gactatccaa gcactagccc tgcaaagagg ggacaacata gtggtcactc
     4681 cttctcagct gacagtggaa gatgaggaca ctccagctga ttttattctt tttaccatca
     4741 cacagatccc catccatggc aggattctat ataatggtag ccgtcctgtg accactttca
     4801 ccaagaagga cctgacaaag agcctgattc tctattgtca cgatggcagt gagactagca
     4861 aagacagttt ctccttcact gtgactgatg gaatccacac aggcttctat gtcttcccag
     4921 acacttccct ggagacacac gtgcctcaga cagtgtggat ccagataagc ccctttgacg
     4981 acaggctccc acagatgggc atcaacaggg gtgccacagc cttgaagctc cttcacactg
     5041 gacacttggg tttcctcatt accaacgagt atttacaagc aacacatcag ggcgttcccc
     5101 acagactcct gacatataaa gtgaccagag gaccggagca tggctacatc gtcaatgctg
     5161 gccttggaaa tgagaacacc cacatgttca ctcaagctga cattgatgag atgcgggtct
     5221 actacatcct gaacaagggc agaggccgcg caacaagaga taccttctac ttctctgttg
     5281 aaacccgtgg aggaaagcag ctaagaaacc agcctttcca tctaaactgg gcttggattt
     5341 ctttggaaaa agaatactac attgtggatg aagactcccc attcttcgaa gtgactctca
     5401 cccgcagggg ataccttgga ggcacatctg tcgttagcat tggaaccaaa ggagacactg
     5461 cagaagaaaa taaggatttt aaaggaaagg ctcctatgct ggtccagttc agtcccggcc
     5521 agtctacagc cacatggagg gtaggactta tacccgatac caagtacgag acctcagaaa
     5581 ctttccagat cattctctct gaacctggcg cggctgcact ggagtttcca gagatggcaa
     5641 cagttgaaat tgtagatcct gaagatgaat caacagttta cattccagag gcagaatacc
     5701 aactggcaga agctgttggg gagtttctgg ttcctgtgag acgatctgga gacacgagcc
     5761 aggagctcac ggtcatctgc tctactcgac caggttctgc cactggcaca attccttccg
     5821 tgcagttatc tttgtcagat tatgtctcaa gaccagaaga taataccagc atgctccact
     5881 ttgaaaagga tgagagcgag aagacctgcc aggtcctgat tatcaatgac tccctttatg
     5941 aggaggaaga gtccttcagc attgcattga gcctgccaac gggagggcag ctgggagcca
     6001 aattccccac tgccagggta acgatcctgg ctgacagaga ggatgagcct gccctgcact
     6061 tcgaatgctc tgagtaccat gctgaggaaa gtgctggcta cgtggaggtg gccgtctgga
     6121 gaagaggcac tggcctttct caagcatctt ctgtcattgt gcgctctagg gggacagagg
     6181 aacaggcagc agaagctgga acagactaca ttggtgtcag gcaacatctg cactttagcc
     6241 caggagtgag tgtgcagaga ctccgagtga caatccttga tgacctgcca cagcctgcct
     6301 ttgagggtcc agagatgttt gaattacttc tccagatgcc tacaggtgca gtgattggag
     6361 aaccaaataa gaccactgtt accatcaatg acagtgtcac caactgtgaa gaggagtgtg
     6421 tgtagttcct tggactaaat aagaccttca attggggaac agtatcctag ttatttccca
     6481 acacaaagcc attccagagc ttagtgactc tgtgtaatga cattgtactg tcagtgtgtt
     6541 ttgtgaaaat agtctatgtc aatgcatgga gatagaaata tatatgcaaa ggattgagcc
     6601 aataaaaaaa aaatgaggtc atgttc
//
