LOCUS       NM_001198811            9546 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus Fras1 related extracellular matrix protein 1 (Frem1),
            transcript variant 2, mRNA.
ACCESSION   NM_001198811
VERSION     NM_001198811.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   McLaughlin,M.T., Sun,M.R., Beames,T.G., Steward,A.C.,
            Theisen,J.W.M., Chung,H.M., Everson,J.L., Moskowitz,I.P.,
            Sheets,M.D. and Lipinski,R.J.
  TITLE     Frem1 activity is regulated by Sonic hedgehog signaling in the
            cranial neural crest mesenchyme during midfacial morphogenesis
  JOURNAL   Dev Dyn 252 (4), 483-494 (2023)
   PUBMED   36495293
  REMARK    GeneRIF: Frem1 activity is regulated by Sonic hedgehog signaling in
            the cranial neural crest mesenchyme during midfacial morphogenesis.
REFERENCE   2  (bases 1 to 9546)
  AUTHORS   Jordan,V.K., Beck,T.F., Hernandez-Garcia,A., Kundert,P.N.,
            Kim,B.J., Jhangiani,S.N., Gambin,T., Starkovich,M., Punetha,J.,
            Paine,I.S., Posey,J.E., Li,A.H., Muzny,D., Hsu,C.W., Lashua,A.J.,
            Sun,X., Fernandes,C.J., Dickinson,M.E., Lally,K.P., Gibbs,R.A.,
            Boerwinkle,E., Lupski,J.R. and Scott,D.A.
  TITLE     The role of FREM2 and FRAS1 in the development of congenital
            diaphragmatic hernia
  JOURNAL   Hum Mol Genet 27 (12), 2064-2075 (2018)
   PUBMED   29618029
REFERENCE   3  (bases 1 to 9546)
  AUTHORS   Fantauzzo,K.A. and Soriano,P.
  TITLE     PDGFRbeta regulates craniofacial development through homodimers and
            functional heterodimers with PDGFRalpha
  JOURNAL   Genes Dev 30 (21), 2443-2458 (2016)
   PUBMED   27856617
REFERENCE   4  (bases 1 to 9546)
  AUTHORS   Tomann,P., Paus,R., Millar,S.E., Scheidereit,C. and
            Schmidt-Ullrich,R.
  TITLE     Lhx2 is a direct NF-kappaB target gene that promotes primary hair
            follicle placode down-growth
  JOURNAL   Development 143 (9), 1512-1522 (2016)
   PUBMED   26952977
REFERENCE   5  (bases 1 to 9546)
  AUTHORS   Wiradjaja,F., Cottle,D.L., Jones,L. and Smyth,I.
  TITLE     Regulation of PDGFC signalling and extracellular matrix composition
            by FREM1 in mice
  JOURNAL   Dis Model Mech 6 (6), 1426-1433 (2013)
   PUBMED   24046351
  REMARK    GeneRIF: loss of FREM1 function promotes epidermal blistering in
            Fraser syndrome as a consequence of reduced PDGFC activity, in
            addition to its stabilising role in the basement membrane
REFERENCE   6  (bases 1 to 9546)
  AUTHORS   Kiyozumi,D., Osada,A., Sugimoto,N., Weber,C.N., Ono,Y., Imai,T.,
            Okada,A. and Sekiguchi,K.
  TITLE     Identification of a novel cell-adhesive protein spatiotemporally
            expressed in the basement membrane of mouse developing hair
            follicle
  JOURNAL   Exp Cell Res 306 (1), 9-23 (2005)
   PUBMED   15878328
  REMARK    GeneRIF: QBRICK is an adhesive ligand of basement membrane
            distinctively recognized by cells in the embryonic skin and hair
            follicles through different types of integrins directed to the
            Arg-Gly-Asp motif.
REFERENCE   7  (bases 1 to 9546)
  AUTHORS   Smyth,I., Du,X., Taylor,M.S., Justice,M.J., Beutler,B. and
            Jackson,I.J.
  TITLE     The extracellular matrix gene Frem1 is essential for the normal
            adhesion of the embryonic epidermis
  JOURNAL   Proc Natl Acad Sci U S A 101 (37), 13560-13565 (2004)
   PUBMED   15345741
  REMARK    GeneRIF: Frem1 appears to act as a dermal mediator of basement
            membrane adhesion and is required for epidermal adhesion during
            embryonic development
REFERENCE   8  (bases 1 to 9546)
  AUTHORS   Takamiya,K., Kostourou,V., Adams,S., Jadeja,S., Chalepakis,G.,
            Scambler,P.J., Huganir,R.L. and Adams,R.H.
  TITLE     A direct functional link between the multi-PDZ domain protein GRIP1
            and the Fraser syndrome protein Fras1
  JOURNAL   Nat Genet 36 (2), 172-177 (2004)
   PUBMED   14730302
REFERENCE   9  (bases 1 to 9546)
  AUTHORS   Brown,K.S., Hetzel,S.C. and Harne,L.C.
  TITLE     Craniofacial consequences of connective tissue disorders in mice
  JOURNAL   Birth Defects Orig Artic Ser 20 (3), 113-136 (1984)
   PUBMED   6391574
  REMARK    Review article
REFERENCE   10 (bases 1 to 9546)
  AUTHORS   Varnum,D.S. and Fox,S.C.
  TITLE     Head blebs: a new mutation on chromosome 4 of the mouse
  JOURNAL   J Hered 72 (4), 293 (1981)
   PUBMED   6793660
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AB160986.1.
            
            On Mar 17, 2018 this sequence version replaced NM_001198811.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB160986.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9546              AB160986.1         2-9547
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 39.4 cM"
     gene            1..9546
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /note="Fras1 related extracellular matrix protein 1"
                     /db_xref="GeneID:329872"
                     /db_xref="MGI:MGI:2670972"
     exon            1..266
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            267..777
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     CDS             526..7044
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2"
                     /codon_start=1
                     /product="FRAS1-related extracellular matrix protein 1
                     isoform 2 precursor"
                     /protein_id="NP_001185740.1"
                     /db_xref="CCDS:CCDS57281.1"
                     /db_xref="GeneID:329872"
                     /db_xref="MGI:MGI:2670972"
                     /translation="MHSPGCTGPKAQWFLLLQLLLLHLDRVSATFISINRGLRVMKGS
                     SAFLSGDHLRVAVPKEKDACRLEVVMNEPVTQRVGKLSPQVFDCHFLPNEVKYVHNGC
                     PILDEDSVKLRLYRFTETDTFMETFLLRVYLVEPDCNIIRMSSNVLEVTEFYGLSQAI
                     DKNLLQFDYDRTASLDCTIRLDPLRTQLPAHGKLVVVNRKSEGPRGDQPHSFFSETEL
                     GAGLKCPDGSCALELKQVASLKVSCEEFLLTGFHYQHMQPPSPNIDYIPIQLDLTDRR
                     SKTVYKSESAWLPVYIRVGIPNQVPRAAFMAMFVLEVDQFILTPLTTSVLDCEEDETP
                     KPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQVAYQPPNSSHPERRHYTME
                     LEVYDFFFERSAPITVHISIRTADTNAPRVSWNTGLNLLEGQSRAITWEQFQIVDNDD
                     IGAVQLVTIGGLQHGRLTVREGKGFLFTVTDLQAGVVRYHHDDSDTTKDFVAFRIFDG
                     HHSSHHKFPINILPKDDSPPFLITNVVIELEEGKTILIQGSMLRASDMDSSDDYIFFN
                     ITKFPQAGEIMKKPGPRLIGYPVPGFLQRDLFSGIIYYRHFGGEIFEDSFEFVLWDSH
                     EPPNLSVPQVVTIHITPVDDQLPKEAPGISRHLVVKETEVAYITKKHLHFLDMESRDG
                     ELIYTVTRPPCFSFSHRHLDAGKLFMVDSIPKLTKNPTAPGLSSFTQHAVNHMKVAYM
                     PPMQDIGPSPRHVQFTVSVSNQHGGALHGICFNITVLPVDNQVPEVFTNTLRVVEGGQ
                     CTISTEHILVSDVDTPLDSISLSLKERPLHGGVELDGFPLNPRGTFSWRDLNTLKVWY
                     QHDGSEVLQDEIFLEVTDGTNSAAFVLHIEVFPVNDEPPILKADLIPMMHCSEGGEVT
                     ITPEYISATDADSDDLELLFLIAREPQHGVVRKAGLHVDRFSQGDVISGAVTYKHTGG
                     EIGLEPCSDTVVLVVSDGEADPLMNGCCYDGPDSSVPLHKSFPTYQLNITVHPVDNQP
                     PSIIIGRMLTVDEGFSAALTTHHLTVVDWDTAPDDLKFMLASQPQFGYLENALPSAGF
                     EKSNIGIRIASFQWTDMKASHINYVQSRHLRVEPTADQFTVYATDGKHRSLETTFHVI
                     INPTNDEAPDLAVQNITVYEGHMVELDSSIISATDRDIPKDPLLFSIALKPQHGLLVD
                     AAISKDSHQIKQLQHEIHSFSVDLLKNGMKLVYAHDDSESSADNFVIQLSDGKHKILK
                     TISVNITPVNDETPTLSKKAEISMAVGDTRVLSSAVLSATDKDSPREKIHYVFERLPQ
                     NGQLQLKIGRDWVPLSTGMQCTQEDVDLNLLRYTHAGKTDSQDGDSFTFYLWDEDNRS
                     PAFDCHIIIEDMGKGDIVIHAKPLVVVKGDRGLLTTATLLAVDGADKPEELLYLITSP
                     PRHGQVEYVHSPGVPIASFSQMDIAGQTVCYIHKSRTAVPTDSFRFTISNGLQTQRGV
                     FEITLQTVDSALPVLTKNKRLRLAEGAMGLLSADHLQLTDPDTPPENLTFFLAQLPRH
                     GYLFLRGKALQHNFTQRDVDSGGVAYQHSGGGAREDYFTFLATDRKNQGFVVDGKVQK
                     EPVRFTIQVDQLDKAAPRITHLHSPTQVGLLKNGCYGIYITSRVLKASDPDTEDDQII
                     FKILRGPLYGRLENTTTGEFIHERFSQKDLSHKTILYIINPSLQVTSDILEFQAMDPT
                     GNTATPQSLDLRWSYIEWAQTAYEVCENVGLLPLEVTRRGYPMDSAFVGVEVNQVSAT
                     VGKDFTVTPSKLLQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTQTKA
                     AVKILDSKGGRCHPSNSFNQSKHSTWGKGPWHPLPSGSSSLTTSGSPLLERPPPSFTS
                     GDALQGFGLTDLTQRKTMTQGNGKSVLPSSVCRNGTDTIYNYHGIVSLKLEGDRFSAH
                     KRKAKISIVSQPQRTIKVAELPLADKVESTTDLHFLRQGLRPLFPKNCSVDLKGLFHF
                     EESTHRLYQCDGISWKAWSPQTKGLEDRSCPGGWLLHSGYCHILVTRQKGTWTTATRA
                     CREQHQGDLVTVLSRRHMQWLWAMSGRKPFWIGLKNQPRTGHWEWIGGEPVAFTNWRR
                     GAPLHPKPGKNCALVQKRGQWQTKNCSKGKAHNFVCSRKL"
     exon            778..872
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            873..1174
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1371
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..1695
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..1804
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..1936
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            1937..2281
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            2282..2424
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            2425..2621
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            2622..2712
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            2713..2880
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            2881..3089
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            3090..3183
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            3184..3436
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            3437..3631
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            3632..3817
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            3818..4014
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            4015..4219
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            4220..4364
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            4365..4506
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            4507..4702
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            4703..4967
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            4968..5379
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            5380..5581
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            5582..5726
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            5727..5856
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            5857..5929
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            5930..6079
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            6080..6300
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            6301..6348
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            6349..6513
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            6514..6642
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            6643..6758
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            6759..6844
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     exon            6845..9523
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9494..9499
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /note="hexamer: AATAAA"
     polyA_site      9514
                     /gene="Frem1"
                     /gene_synonym="crf11; D430009N09; D630008K06; eye<m02Jus>;
                     eyes2; heb; qbrick"
                     /note="major polyA site"
ORIGIN      
        1 acagcggcat tggaggagac ctgcaaacag tgtgtttggg aactccaatt ctctacctat
       61 ttgtttgtgc gggttaaagg atttttagca caaacaaagc ctaaactcag ctgactagaa
      121 cgactggaat ttcagctctc ctccttggat gggatgtttt agcttcctct tcctctgaca
      181 actctttaag gctgttacca ggttagcatc cagccctcta cttccaaggg tggccagccc
      241 ttggctcgga aagaaaacca ggaactgatt aggaaggcac ttgggaggca cattaatctt
      301 gccagcgttg tcaggaatga tcagcgagtt ttattaaaaa gccccgattg cctgaaaggc
      361 ctccttgtct gaatctgaat ggaaggagca tgttaaggtc ttggggcacg ctgtcgggac
      421 ccctagtatt gccccctgga cctcaagctg acacttgggg aagaagtgca ggaagcttgc
      481 tttgttgaac agactgtgcc tgtggacaga gctggccctg gcatcatgca ctctccaggc
      541 tgcactgggc ccaaggctca gtggttcttg ctgctccagc tgcttctgct gcacctggac
      601 cgggtcagtg ccaccttcat cagcatcaac cggggcctga gggtgatgaa gggtagttct
      661 gccttcctgt ctggagatca cctgagggtt gcagtcccta aagaaaagga cgcctgcaga
      721 ctggaggtgg tgatgaacga gcccgtaacc cagagggttg ggaaactctc tccacaggtc
      781 tttgattgcc attttcttcc caacgaagtg aagtatgttc acaatggatg cccaattctg
      841 gatgaagact ctgtgaagct tagactttac aggtttactg aaacagacac cttcatggaa
      901 acctttctct tgcgggttta tcttgtggaa ccagactgta acatcatccg tatgagcagt
      961 aatgtgttgg aagtgactga attctatggc ttatcgcaag ccattgataa gaacctgctc
     1021 cagtttgatt atgataggac tgccagcctg gattgcacca tcagactaga cccactgagg
     1081 actcagctgc cagctcacgg caagctggtc gtggtgaatc gtaagtcaga ggggcctcgt
     1141 ggagatcagc cacacagctt cttctctgag acagagctag gggctggact gaagtgtcca
     1201 gatggaagct gtgccctgga attgaagcaa gtggcaagtc tgaaagtgag ctgtgaggag
     1261 tttctgctga cggggtttca ctaccaacac atgcagcccc cttcacccaa cattgattac
     1321 atccccattc agttggatct caccgacaga aggagcaaaa ccgtatacaa gtcagagagt
     1381 gcgtggttgc ctgtctatat cagagttggc attcctaacc aggtcccaag ggctgcattt
     1441 atggccatgt ttgttctgga ggtggaccag ttcatactga cccctctgac aacctccgtt
     1501 ctggactgtg aggaggatga gactccgaaa cccttgttgg tgttcaacat cactaaagcc
     1561 ccactccagg gctatgtgac ccacctgctg gatcatacca gacccatctc ttcattcacc
     1621 tggaaggatc tcagtgacat gcaagttgcc taccagccac caaacagcag ccaccctgag
     1681 aggagacatt acacgatgga gctggaggtg tacgacttct tctttgagag gagtgccccc
     1741 atcactgtcc acatctccat cagaacagca gacacgaatg caccacgggt gtcttggaat
     1801 acaggtttga atctcctaga ggggcagtct cgggccatca cttgggaaca atttcagatc
     1861 gttgacaatg atgacattgg tgctgtgcag ttggtcacca ttggtggcct gcagcatgga
     1921 cggcttacag tacgagaggg gaaaggattt ctcttcaccg tgactgacct tcaagctgga
     1981 gttgtccgct atcatcacga tgacagtgat accaccaaag actttgtggc tttcaggata
     2041 ttcgatggcc accacagcag ccaccacaag tttcctatca acatcttgcc taaagatgac
     2101 agccccccat tcctcatcac caatgtcgtg attgaactag aagaggggaa gaccatcttg
     2161 atccaagggt ctatgttgag agcttcagat atggactcca gtgatgacta catcttcttt
     2221 aatatcacaa agtttccaca ggctggggag atcatgaaga agccagggcc aagactgata
     2281 gggtatcctg tccctggctt cctgcagaga gacctgttca gtggaatcat ttactatcgt
     2341 cactttggtg gagaaatctt tgaagattcc tttgaattcg tactgtggga cagccatgag
     2401 cctccaaatc tctcagtgcc acaggtggtg acaattcata tcactccagt agatgaccag
     2461 cttcctaaag aagctcctgg catttcccgg catttggttg tcaaggaaac agaggtggcc
     2521 tacatcacta aaaagcacct acatttccta gacatggaat cccgtgatgg ggaactcatc
     2581 tacacagtaa cacgccctcc atgtttctcc ttcagccaca gacacttgga tgctgggaag
     2641 ctattcatgg tggacagcat accaaagcta accaaaaatc cgacagcccc agggctaagt
     2701 tcatttactc agcatgccgt gaaccatatg aaagtggcct acatgccacc catgcaagat
     2761 ataggcccct cccctcgaca tgtgcagttc acagtgtctg tcagtaacca gcatggaggt
     2821 gctttgcatg gaatctgctt caacatcaca gttctcccag tggacaacca ggttcccgag
     2881 gtattcacca acaccctgag agtggtcgag ggcggtcagt gcaccatcag cacagagcac
     2941 attttggttt ctgatgtgga caccccgcta gacagcatca gcctctctct taaggagagg
     3001 cctctgcatg gcggggtaga gctggacgga tttcctctaa acccaagagg cacattttct
     3061 tggcgagacc tcaatacctt aaaagtttgg tatcaacatg atggatctga ggttcttcag
     3121 gatgagatat ttttggaggt cacagatggc acaaattcag cagcatttgt tctccacatt
     3181 gaggtgttcc ctgtgaatga tgagccaccc attctgaagg ctgacctcat ccccatgatg
     3241 cactgctctg agggtggaga ggtgaccatc acccctgaat acatttccgc tactgatgca
     3301 gacagcgatg acttagaact gctgtttctg attgcccgag agcctcagca tggagtggtg
     3361 agaaaggctg gacttcacgt ggacaggttc tctcagggag atgtcatctc aggggctgtg
     3421 acatacaaac atacaggtgg cgagattggc cttgaacctt gttctgacac tgttgtattg
     3481 gtggtttcgg atggtgaagc tgaccctttg atgaacggct gttgctacga tggacctgat
     3541 tcatctgttc ctcttcataa gtccttcccc acataccagc tcaacatcac agtacatcca
     3601 gtcgacaacc agccaccttc aattataata ggtaggatgc tcacagtgga tgaaggcttc
     3661 tcagcagccc ttactactca tcacttgact gttgttgatt gggacactgc cccggatgac
     3721 ttaaaattca tgttggcttc tcagccccag tttggctact tagaaaacgc actgccttct
     3781 gcaggctttg agaaaagtaa catcggcata aggatagcct cattccagtg gacagacatg
     3841 aaggcttcac acattaacta tgtgcagtcc aggcacctga gagtggaacc aactgctgac
     3901 cagttcacag tatatgccac agacgggaag catcgctcct tggagacaac atttcacgtt
     3961 atcatcaacc ccacaaacga tgaagctcct gaccttgcag tacagaacat cactgtgtat
     4021 gaggggcaca tggtagaact ggattcttcc atcatcagtg ctactgacag ggatattccc
     4081 aaggacccct tgctcttcag catcgctctc aagccccagc acggcctgct cgtcgatgct
     4141 gccatcagca aggactccca tcagatcaag cagctgcaac acgaaatcca cagcttctcc
     4201 gtggaccttc tcaagaatgg aatgaagctg gtctatgccc acgacgactc agagagctct
     4261 gctgataact tcgtgatcca gttgtcagac gggaaacata aaatccttaa aaccatctca
     4321 gtaaacatta ccccagtgaa tgacgagaca ccaacgctaa gcaagaaggc tgaaatctca
     4381 atggctgtgg gcgatactcg cgtcctgtct agtgctgttc tttcagccac agataaagac
     4441 tcgcccagag agaagattca ctatgtattt gaaagacttc cccaaaatgg acaacttcaa
     4501 cttaagatag ggagggactg ggtccctctc tccactggca tgcagtgcac ccaggaggat
     4561 gtggacctga acttgctaag atacacgcat gctggtaaaa cggactccca ggatggagac
     4621 agcttcacct tctacctctg ggatgaggac aacaggtcac ctgcatttga ctgtcacatc
     4681 atcattgaag acatgggcaa aggtgatatt gtcattcatg caaaaccact cgtggtagtc
     4741 aaaggtgaca gaggtctctt gacgactgcc acccttctgg ctgtggatgg agcagacaag
     4801 cctgaggaac tgctctacct catcacctcc ccaccacggc atggccaagt ggagtacgtc
     4861 cactctcctg gagtccccat tgccagcttc agccaaatgg acatagcagg acagacagtg
     4921 tgctacatac acaagagcag gacagctgtc cccactgaca gcttcagatt taccatcagc
     4981 aatggactgc agacccagcg tggggtgttt gaaatcacac tgcagactgt ggacagcgcc
     5041 ttgcctgtgc tgaccaagaa caaaaggctg aggctagccg aaggggccat gggcctcctg
     5101 tccgctgatc accttcagct gactgacccc gacacacctc cagagaacct gaccttcttt
     5161 ctggctcagc tcccacgcca cgggtacctc ttcctgagag ggaaagcact ccaacacaac
     5221 ttcacccagc gagatgtgga cagcgggggc gtggcctacc agcactcagg aggcggcgcc
     5281 cgggaggact attttacttt tctagccaca gacaggaaga accaaggctt tgttgtggat
     5341 gggaaagttc agaaagagcc tgttcgcttc acaatccagg tggatcagct ggacaaggca
     5401 gcgccccgta tcacacactt gcactcccct actcaagtgg ggctcttgaa aaatggctgc
     5461 tatgggattt acatcacttc ccgtgtgctg aaggcatcag accctgacac agaggatgac
     5521 cagatcatct ttaagatttt acgaggccca ttgtacggac gtctggagaa cacaacaaca
     5581 ggtgaattca tccacgagcg atttagccag aaggacttaa gccataagac catcctttac
     5641 atcataaacc cttctttgca agtgacttcc gatatcctgg aatttcaggc catggacccc
     5701 acagggaaca ctgccactcc ccagagcttg gacttgagat ggtcgtatat tgaatgggcc
     5761 cagactgcat atgaggtttg tgagaatgtg ggcttattgc ccttggaagt taccagaagg
     5821 ggatatccca tggactcagc ctttgtgggt gtagaggtta accaagtgtc agctacagtt
     5881 ggaaaagatt ttactgtcac tccatccaaa ctgcttcaat ttgacccagg aatgtcaact
     5941 aagatgtgga atatagcaat tacctatgac ggattagagg aagatgatga ggtctttgaa
     6001 gtaattctga actcccctgt gaatgcggtt cttggcacgc agacaaaagc ggcggtgaaa
     6061 attttggact caaaaggagg acggtgccat ccttcaaatt ccttcaacca aagcaagcac
     6121 agcacgtggg ggaagggccc ttggcatccg ctgccctcag gctcatcctc gctcaccact
     6181 tcaggttctc ctcttctgga aaggcctcct ccatctttca ccagtggaga tgctcttcag
     6241 ggttttggtc tcaccgacct cactcaaagg aagacaatga cccaggggaa tggcaaatca
     6301 gttctcccat cctctgtttg tagaaatggg acagacacca tctacaatta ccatggtata
     6361 gtttccttga aactagaggg cgacagattc tcagctcaca aacggaaggc caaaatatcc
     6421 attgttagtc agccacagag gacaatcaag gtggcagagc tgcctctagc tgataaggtg
     6481 gaatccacaa ctgatttgca ctttctcaga cagggtctgc ggcccttgtt tccaaagaat
     6541 tgttctgtgg acttaaaggg actctttcat tttgaagaaa gcacccacag attgtatcaa
     6601 tgtgatggga tctcctggaa agcctggagc ccccaaacca aggggctgga ggacagatcc
     6661 tgcccaggtg gatggctcct tcattctggc tactgtcaca tcttggtcac aagacagaaa
     6721 ggcacctgga ccacagctac ccgagcttgc agagaacagc accagggcga ccttgtgacc
     6781 gtactctcca gaaggcatat gcagtggcta tgggcgatga gtggaagaaa acccttctgg
     6841 ataggcttga agaaccaacc gaggactggc cactgggagt ggatcggagg tgaacctgtt
     6901 gcttttacca actggaggag aggggcccct ctgcacccaa aacctggaaa gaactgtgct
     6961 ttggttcaga aaagaggaca atggcagaca aagaactgta gcaaaggcaa ggctcacaat
     7021 tttgtgtgct caaggaaact ctgaacacaa cctagccttt ctcattcatc tatttacaag
     7081 atctatgtgt attgcccagt gaagatgcgt tgttgtggct ggctgggcat gtctttgtga
     7141 gtctgtcaag caaacctgga agctaaatca acagtcccag ggagatgact gtatgaatgt
     7201 gcctttcaag atgagataca acttctgtat ctcaatattt ttctagataa atgtctacag
     7261 tattataggc taaaatattt gcttcttcta gaagattcag actctttcct atgcctactc
     7321 tcaggcaata caaatatcca tggaccttaa atgcttttgt ctgtagacct ccaatgagtc
     7381 cacctagaaa ggtcggtatt gcaggtgctt ctaccacaga gtttgagtgg atatagttct
     7441 gtagatccat taaaccaagg ttgaatgcca aaggttggtt tatttcctta ggccctttaa
     7501 atgtgaactt aaaaccagag gctatgtgta gacaaagggg ctctgttttg aatggaagag
     7561 tctttcaaga ggatgctcaa ctatataggt ttagtgaggt agggtcagag gcctgcctct
     7621 attggagagc tcagattcag gcaaggctct gactctacta gggagatcaa cccattccac
     7681 tcgtgagaca tgttacattc caatacaatt atggcattgt gccattcctt gactaactat
     7741 tgggttttca caccttccaa ggtcgtataa actttgaagt agcaatatct tcatctctat
     7801 ttactatgtt gacaagtctt tttaaaggat atgtggaata aggagtgtat atgagtatgg
     7861 ttgacataca gaggaaaagt gccactccct tggaagtacc ttcatgaact gttctgatga
     7921 ccaaagaaaa gtaatactgg aaacagacct tttgaaaggc cagactcact cttggaaagg
     7981 attttgatgt gactttgagc ctttaaatct gtaatatttg cagagacact cttctaatta
     8041 gattctccaa tagagggtag cttattaatt tataaactca gttgagtgag acatttttgt
     8101 agcattcaag catgctgtgc ttctcccaac aatcccttct ggagcccagg gttaattata
     8161 ggaagtcaga acgtcctttt tgaaatgttt attaaaattt ttcttttcat aatctctaaa
     8221 aaatacagct gtgccaaata taacctgtca tatgagcacc cgtgtaactt tagaattaaa
     8281 tgaggtgttc aagtagacta gagctttagg gcagactttt taaatagaat aataatatct
     8341 cgagtgttat agacactaaa aatggcttaa gaaagtcgtt ccagtttctg gatataaaac
     8401 atgacaggct tgcttcattt ctgctcattt atcaaagttt cggttcatag aaatttttac
     8461 ctaaggttag gataagactc agacattctg tctcttgctg aaatccagat gtaattgcag
     8521 agccgggtgc tcttagcttg gaggtttgga gaagtttgta gaccgtaagt gcaaaggcct
     8581 gtggaacata aggaaccttt agtgtttaac ccaaacaggt caaatccttc ctccactcgg
     8641 gctccagcca agctccttgt aacctactgg cttctacact gtgcaaagtt gggattcagc
     8701 gtgcaggaaa gagggtggag ttctccagtt cggatactga ggaattcatt ctatagctgt
     8761 gtgctccgtt aaagctggaa atacagaact aggccaaccg tgctcaacct gcgcggcaca
     8821 gctagtttat tacactttcc tgcactgaaa atggcatggc tgtaattgca agtggttagt
     8881 tagtataggg aatggtttgg gaagagaccc aattagtttc ttccaattag gaaaagacca
     8941 ttttgaaatt ttcttgatca tgaaagcttt accaaaatgt ggaatcgggt ctaagtgatt
     9001 ggcagaacac acaaggtcag gcaagcttag ctgatggaca tggctgtcag ggttgaggag
     9061 gcaaggctgg agatgccagt agccaagaag aacatacagc agagggctga ttattgccct
     9121 tccccctcta acttgaatgc ccactcagga gcttcttatg tgcaaggaca tacatgaagc
     9181 tcaatggtat agaactttta aattttgttc tgatagttga tccttaaaga atacatagta
     9241 tatgtttact gaagataaat cttaaaagct ggcgccaatc atttcctcct cttccctgga
     9301 gtgatacaaa gaaactgagc aacctttatt tgtatttcta gtttagttta tttttaatac
     9361 aacccaaata tgcacgtatt ttaatgaaca ctgtggttct atgtaattta aatcatcaac
     9421 tggtgctgac taatttctgt aaggaagtag catattaact gtactatatc tagtgttgtg
     9481 tcaaagccaa taaaataaac atgtctaatt ctcagacact gtaaaaaaaa aaaaaaaaaa
     9541 aaaaaa
//
