LOCUS       NM_010236               2265 bp    mRNA    linear   ROD 09-DEC-2022
DEFINITION  Mus musculus folylpolyglutamyl synthetase (Fpgs), mRNA; nuclear
            gene for mitochondrial product.
ACCESSION   NM_010236
VERSION     NM_010236.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Yoo D, Park J, Lee C, Song I, Lee YH, Yun T, Lee H, Heguy A, Han
            JY, Dasen JS, Kim H and Baek M.
  TITLE     Little skate genome provides insights into genetic programs
            essential for limb-based locomotion
  JOURNAL   Elife 11, e78345 (2022)
   PUBMED   36288084
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Yang C, Xie LY, Windle JJ, Taylor SM and Moran RG.
  TITLE     Humanizing mouse folate metabolism: conversion of the dual-promoter
            mouse folylpolyglutamate synthetase gene to the human
            single-promoter structure
  JOURNAL   FASEB J 28 (5), 1998-2008 (2014)
   PUBMED   24532667
REFERENCE   3  (bases 1 to 2265)
  AUTHORS   Sakamoto E, Tsukioka S, Oie S, Kobunai T, Tsujimoto H, Sakamoto K,
            Okayama Y, Sugimoto Y, Oka T, Fukushima M and Oka T.
  TITLE     Folylpolyglutamate synthase and gamma-glutamyl hydrolase regulate
            leucovorin-enhanced 5-fluorouracil anticancer activity
  JOURNAL   Biochem Biophys Res Commun 365 (4), 801-807 (2008)
   PUBMED   18035049
  REMARK    GeneRIF: These results suggest that FPGS and GGH expression levels
            in tumors are determinants of the efficacy of LV in enhancing the
            antitumor activity of 5-FU.
REFERENCE   4  (bases 1 to 2265)
  AUTHORS   Racanelli AC, Turner FB, Xie LY, Taylor SM and Moran RG.
  TITLE     A mouse gene that coordinates epigenetic controls and
            transcriptional interference to achieve tissue-specific expression
  JOURNAL   Mol Cell Biol 28 (2), 836-848 (2008)
   PUBMED   17998333
REFERENCE   5  (bases 1 to 2265)
  AUTHORS   Delano DL, Montesinos MC, Desai A, Wilder T, Fernandez P,
            D'Eustachio P, Wiltshire T and Cronstein BN.
  TITLE     Genetically based resistance to the antiinflammatory effects of
            methotrexate in the air-pouch model of acute inflammation
  JOURNAL   Arthritis Rheum 52 (8), 2567-2575 (2005)
   PUBMED   16059892
REFERENCE   6  (bases 1 to 2265)
  AUTHORS   Roy K, Mitsugi K, Sirlin S, Shane B and Sirotnak FM.
  TITLE     Different antifolate-resistant L1210 cell variants with either
            increased or decreased folylpolyglutamate synthetase gene
            expression at the level of mRNA transcription
  JOURNAL   J Biol Chem 270 (45), 26918-26922 (1995)
   PUBMED   7592937
REFERENCE   7  (bases 1 to 2265)
  AUTHORS   Pilz A, Woodward K, Povey S and Abbott C.
  TITLE     Comparative mapping of 50 human chromosome 9 loci in the laboratory
            mouse
  JOURNAL   Genomics 25 (1), 139-149 (1995)
   PUBMED   7774911
REFERENCE   8  (bases 1 to 2265)
  AUTHORS   Rowe LB, Nadeau JH, Turner R, Frankel WN, Letts VA, Eppig JT, Ko
            MS, Thurston SJ and Birkenmeier EH.
  TITLE     Maps from two interspecific backcross DNA panels available as a
            community genetic mapping resource
  JOURNAL   Mamm Genome 5 (5), 253-274 (1994)
   PUBMED   8075499
  REMARK    Erratum:[Mamm Genome 1994 Jul;5(7):463]
REFERENCE   9  (bases 1 to 2265)
  AUTHORS   Levan G, Szpirer J, Szpirer C, Klinga K, Hanson C and Islam MQ.
  TITLE     The gene map of the Norway rat (Rattus norvegicus) and comparative
            mapping with mouse and man
  JOURNAL   Genomics 10 (3), 699-718 (1991)
   PUBMED   1889815
REFERENCE   10 (bases 1 to 2265)
  AUTHORS   Fournier,R.E. and Moran,R.G.
  TITLE     Complementation mapping in microcell hybrids: localization of Fpgs
            and Ak-1 on Mus musculus chromosome 2
  JOURNAL   Somatic Cell Genet 9 (1), 69-84 (1983)
   PUBMED   6687641
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY025719.1, CA558410.1, CF749232.1, BM944126.1 and BE627294.1.
            
            [WARNING] On Apr 25, 2023 this sequence was replaced by
            NM_010236.3.
            
            On Apr 5, 2008 this sequence version replaced NM_010236.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U33557.1, AK157040.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164143 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-211               BY025719.1         2-212
            212-602             CA558410.1         65-455
            603-1149            CF749232.1         202-748
            1150-1738           BM944126.1         149-737
            1739-2265           BE627294.1         1-527               c
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 22.09 cM"
     gene            1..2265
                     /gene="Fpgs"
                     /note="folylpolyglutamyl synthetase"
                     /db_xref="GeneID:14287"
                     /db_xref="MGI:MGI:95576"
     exon            1..187
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     CDS             50..1813
                     /gene="Fpgs"
                     /EC_number="6.3.2.17"
                     /note="folylpolyglutamate synthase, mitochondrial;
                     tetrahydrofolate synthase; folylpoly-gamma-glutamate
                     synthetase; tetrahydrofolylpolyglutamate synthase"
                     /codon_start=1
                     /product="folylpolyglutamate synthase, mitochondrial
                     precursor"
                     /protein_id="NP_034366.2"
                     /db_xref="CCDS:CCDS15926.1"
                     /db_xref="GeneID:14287"
                     /db_xref="MGI:MGI:95576"
                     /translation="MSWARSRLCSTLSLAAVSARGATTEGAARRGMSAWPAPQEPGME
                     YQDAVRTLNTLQTNASYLEQVKRQRSDPQAQLEAMEMYLARSGLQVEDLNRLNIIHVT
                     GTKGKGSTCAFTERILRNYGLKTGFFSSPHMVQVRERIRINGKPISPELFTKHFWCLY
                     NQLEEFKDDSHVSMPSYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAFDCTNIIRKPVV
                     CGVSSLGIDHTSLLGDTVEKIAWQKGGIFKPGVPAFTVVQPEGPLAVLRDRAQQIGCP
                     LYLCPPLEALEEVGLPLSLGLEGAHQRSNAALALQLAHCWLERQDHQDIQELKVSRPS
                     IRWQLPLAPVFRPTPHMRRGLRDTVWPGRTQILQRGPLTWYLDGAHTTSSVQACVHWY
                     RQSLERSKRTDGGSEVHILLFNSTGDRDSAALLKLLQPCQFDYAVFCPNVTEVSSIGN
                     ADQQNFTVTLDQVLLRCLQHQQHWNGLAEKQASSNLWSSCGPDPAGPGSLLLAPHPPQ
                     PTRTSSLVFSCISHALLWISQGRDPIFQPQSLPRNLLNHPTANSGASILREAAAIHVL
                     VTGSLHLVGGVLKLLDPSMSQ"
     exon            188..316
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            317..370
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            371..435
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            436..550
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            551..628
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            629..690
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            691..793
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            794..871
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            872..1019
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1109
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1260
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1336
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1403
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..2263
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gaagcggggc ggctccggcc caggcctagc ttgcggtggc attaagacta tgtcgtgggc
       61 gcggagccga ctgtgctcga ctctgtccct ggcagctgtt tctgcgcgtg gtgcaacgac
      121 ggagggcgcg gcgcggcggg ggatgagcgc gtggccagcg ccacaggagc cgggcatgga
      181 gtatcaggat gctgtgcgca cgctcaacac cctgcaaacc aatgccagct acctggagca
      241 ggtaaagcgc caacggagtg acccccaggc gcagctggag gctatggaga tgtacctggc
      301 acggagtgga ctgcaggtgg aggacttgaa ccggctaaac attattcatg tcactgggac
      361 caaagggaag ggctccacct gtgccttcac cgaacggatc ctgcggaatt acggcctgaa
      421 gaccggcttc tttagctctc ctcacatggt gcaggtgcgg gagcggattc gaatcaacgg
      481 gaaaccaatc agccccgagc tcttcaccaa gcacttctgg tgcctctata accagctgga
      541 ggagttcaag gacgacagcc atgtctccat gccctcttac ttccgcttcc tcacactcat
      601 ggccttccat gtcttcctcc aagagaaggt ggacctggca gtggtggagg tgggcattgg
      661 cggggctttt gactgcacca acatcatcag aaagccagtg gtgtgtggag tctcctctct
      721 tggcattgac cacaccagtc tactaggaga tacagtggag aaaatagcat ggcagaaagg
      781 gggcatcttt aagcctggtg tccctgcctt cactgtggtg cagccagaag gtcccctggc
      841 tgtgctgagg gatcgagccc agcagattgg atgcccgttg tacctgtgtc cgccattgga
      901 agccctggag gaggttggac tgccattgag cctgggtctg gagggagcac accagcggtc
      961 taatgctgcc ttggccttgc agctggccca ctgttggctg gagcggcagg accaccaaga
     1021 catccaggag ctgaaggtat ccaggccaag catacggtgg cagctgcccc tggcacctgt
     1081 gttccgccct acccctcaca tgaggcgtgg gcttcgggac acagtgtggc ctggccggac
     1141 acagatactc cagcggggac cccttacctg gtacctggat ggcgcccata ccaccagcag
     1201 tgtgcaggcc tgtgtgcact ggtaccgcca gtcattggag cgcagcaaac gcaccgatgg
     1261 agggtccgaa gtacacatct tgctcttcaa ctctactggt gacagggact ctgctgccct
     1321 gctgaagctg ctgcagccct gccagtttga ctacgctgtc ttctgcccca acgtgacaga
     1381 ggtttcatcc ataggaaatg cagaccagca gaacttcact gtgactctgg accaggtgct
     1441 gctccgctgc ctccaacacc agcagcattg gaacggcctg gctgagaaac aggccagctc
     1501 caacctctgg agcagctgcg gcccagaccc tgctgggcca ggctccctgc tgctggcccc
     1561 gcacccacct cagcctacta ggacgagctc cctcgttttc agctgcatct cccacgcctt
     1621 gctgtggatc agccaaggcc gggatcccat ctttcagccc cagagccttc caaggaatct
     1681 tctcaaccac cccacagcca acagcggggc cagcattctc cgtgaggctg ctgccatcca
     1741 tgtactggtt acaggaagcc tgcacctggt gggcggggtt ctgaaactgc tggatccctc
     1801 tatgtcccag tagccaagga ccatcctaca tcggtctgcc tttccacaga ctcttatact
     1861 cagtgccttg tgatttctgc tctcagattt tttcggactg gcccagggtc ctgggctctt
     1921 ggtagagtgt agggatggga gaggctctct gtacctcggc ctctccttcc tctggcagag
     1981 acagcagggt gcttcccaga gtccccacca tcgtagcagg ctttcagcct ggcccatctc
     2041 cctgctgcct ccaggctcag gtccagctta ctgttgcagc tgcctgagca gcctggatcc
     2101 tgcctgaggt tagaccagag acctcctcct ccctcccaat gctttctggc aaaagagagg
     2161 gcccttgcct ggcatttggg gactgtgttg ctggaactaa ttgaagcttt taaacccttt
     2221 tattttttat tttttgtaaa taaatgacaa aacttttgat tgaaa
//
