LOCUS       NM_001403612            3880 bp    mRNA    linear   ROD 06-NOV-2024
DEFINITION  Mus musculus formin-like 1 (Fmnl1), transcript variant 3, mRNA.
ACCESSION   NM_001403612 XM_006533837
VERSION     NM_001403612.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Sigler,A.L., Thompson,S.B., Ellwood-Digel,L., Kandasamy,A.,
            Michaels,M.J., Thumkeo,D., Narumiya,S., Del Alamo,J.C. and
            Jacobelli,J.
  TITLE     FMNL1 and mDia1 promote efficient T cell migration through complex
            environments via distinct mechanisms
  JOURNAL   Front Immunol 15, 1467415 (2024)
   PUBMED   39430739
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Walch,P., Selkrig,J., Knodler,L.A., Rettel,M., Stein,F.,
            Fernandez,K., Vieitez,C., Potel,C.M., Scholzen,K., Geyer,M.,
            Rottner,K., Steele-Mortimer,O., Savitski,M.M., Holden,D.W. and
            Typas,A.
  TITLE     Global mapping of Salmonella enterica-host protein-protein
            interactions during infection
  JOURNAL   Cell Host Microbe 29 (8), 1316-1332 (2021)
   PUBMED   34237247
REFERENCE   3  (bases 1 to 3880)
  AUTHORS   Thompson,S.B., Sandor,A.M., Lui,V., Chung,J.W., Waldman,M.M.,
            Long,R.A., Estin,M.L., Matsuda,J.L., Friedman,R.S. and Jacobelli,J.
  TITLE     Formin-like 1 mediates effector T cell trafficking to inflammatory
            sites to enable T cell-mediated autoimmunity
  JOURNAL   Elife 9, e58046 (2020)
   PUBMED   32510333
  REMARK    GeneRIF: Formin-like 1 mediates effector T cell trafficking to
            inflammatory sites to enable T cell-mediated autoimmunity.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3880)
  AUTHORS   Miller,M.R., Miller,E.W. and Blystone,S.D.
  TITLE     Non-canonical activity of the podosomal formin FMNL1gamma supports
            immune cell migration
  JOURNAL   J Cell Sci 130 (10), 1730-1739 (2017)
   PUBMED   28348104
REFERENCE   5  (bases 1 to 3880)
  AUTHORS   Pfisterer,S.G., Gateva,G., Horvath,P., Pirhonen,J., Salo,V.T.,
            Karhinen,L., Varjosalo,M., Ryhanen,S.J., Lappalainen,P. and
            Ikonen,E.
  TITLE     Role for formin-like 1-dependent acto-myosin assembly in lipid
            droplet dynamics and lipid storage
  JOURNAL   Nat Commun 8, 14858 (2017)
   PUBMED   28361956
  REMARK    GeneRIF: FMNL1 is required for actin assembly on lipid droplets
            (LDs) in vitro and for NMIIa recruitment to LDs in cells. We
            propose a novel acto-myosin structure regulating lipid storage:
            FMNL1-dependent assembly of myosin II-functionalized actin
            filaments on LDs facilitates their dissociation, thereby affecting
            LD surface-to-volume ratio and enzyme accessibility to
            triglycerides.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3880)
  AUTHORS   Seth,A., Otomo,C. and Rosen,M.K.
  TITLE     Autoinhibition regulates cellular localization and actin assembly
            activity of the diaphanous-related formins FRLalpha and mDia1
  JOURNAL   J Cell Biol 174 (5), 701-713 (2006)
   PUBMED   16943183
REFERENCE   7  (bases 1 to 3880)
  AUTHORS   Harris,E.S., Rouiller,I., Hanein,D. and Higgs,H.N.
  TITLE     Mechanistic differences in actin bundling activity of two mammalian
            formins, FRL1 and mDia2
  JOURNAL   J Biol Chem 281 (20), 14383-14392 (2006)
   PUBMED   16556604
  REMARK    GeneRIF: the bundling activities of FRL1 and mDia2, while producing
            phenotypically similar bundles, differ in mechanistic detail
REFERENCE   8  (bases 1 to 3880)
  AUTHORS   Zhou,F., Leder,P. and Martin,S.S.
  TITLE     Formin-1 protein associates with microtubules through a peptide
            domain encoded by exon-2
  JOURNAL   Exp Cell Res 312 (7), 1119-1126 (2006)
   PUBMED   16480715
  REMARK    GeneRIF: Binding to interphase microtubules through exon-2-encoded
            domain of Fmn1 provides a novel mechanism by which Fmn1 isoform Ib
            could coordinate actin filaments and microtubules.
REFERENCE   9  (bases 1 to 3880)
  AUTHORS   Harris,E.S., Li,F. and Higgs,H.N.
  TITLE     The mouse formin, FRLalpha, slows actin filament barbed end
            elongation, competes with capping protein, accelerates
            polymerization from monomers, and severs filaments
  JOURNAL   J Biol Chem 279 (19), 20076-20087 (2004)
   PUBMED   14990563
REFERENCE   10 (bases 1 to 3880)
  AUTHORS   Yayoshi-Yamamoto,S., Taniuchi,I. and Watanabe,T.
  TITLE     FRL, a novel formin-related protein, binds to Rac and regulates
            cell motility and survival of macrophages
  JOURNAL   Mol Cell Biol 20 (18), 6872-6881 (2000)
   PUBMED   10958683
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL662804.18.
            
            On Apr 1, 2022 this sequence version replaced XM_006533837.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13422598.876778.1,
                                           SRR13422601.1324077.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-354               AL662804.18        36604-36957
            355-438             AL662804.18        45032-45115
            439-552             AL662804.18        46398-46511
            553-626             AL662804.18        47225-47298
            627-710             AL662804.18        47602-47685
            711-836             AL662804.18        48158-48283
            837-893             AL662804.18        48532-48588
            894-1002            AL662804.18        51137-51245
            1003-1079           AL662804.18        51927-52003
            1080-1173           AL662804.18        52129-52222
            1174-1248           AL662804.18        55442-55516
            1249-1359           AL662804.18        55812-55922
            1360-1509           AL662804.18        57531-57680
            1510-1611           AL662804.18        58195-58296
            1612-1864           AL662804.18        58466-58718
            1865-2152           AL662804.18        58964-59251
            2153-2277           AL662804.18        59444-59568
            2278-2480           AL662804.18        60142-60344
            2481-2715           AL662804.18        60775-61009
            2716-2784           AL662804.18        61126-61194
            2785-2865           AL662804.18        61777-61857
            2866-2995           AL662804.18        61932-62061
            2996-3159           AL662804.18        62201-62364
            3160-3261           AL662804.18        62594-62695
            3262-3342           AL662804.18        62831-62911
            3343-3463           AL662804.18        63174-63294
            3464-3880           AL662804.18        63999-64415
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 66.71 cM"
     gene            1..3880
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /note="formin-like 1"
                     /db_xref="GeneID:57778"
                     /db_xref="MGI:MGI:1888994"
     exon            1..354
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     CDS             226..3567
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /note="isoform 3 is encoded by transcript variant 3;
                     lymphocyte specific formin related protein; formin-like
                     protein 1; formin-related protein; formin-related gene in
                     leukocytes"
                     /codon_start=1
                     /product="formin-like protein 1 isoform 3"
                     /protein_id="NP_001390541.1"
                     /db_xref="GeneID:57778"
                     /db_xref="MGI:MGI:1888994"
                     /translation="MGNAAGSAEQPAGPTASPPKQPAVPKQPMPAAGELEERFTRVLN
                     CMNLPPDKVQLLSQYDNEKKWELICDQERFQVKNPPAAYIQKLKSYLDTGGVSRKVAS
                     DWMSNLGFKRRVQESTQVLRELETSLRTNHIGWVQEFLNEENRGLDVLLEYLAFAQCS
                     VAYDMESTDSVASGAEKSKPLDQSVEDLSKAPPSSVPKSRLTIKCPPSPRYVHRHLGA
                     GACWGLTPAHSRKALRNSRIVSQKDDVHVCIMCLRAIMNYQSGFSLVMNHPACVNEIA
                     LSLNNKSPRTKALVLELLAAVCLVRGGHDIILAAFDNFKEVCGEQHRFEKLMEYFRHE
                     DSNIDFMVACMQFINIVVHSVENMNFRVFLQYEFTHLGLDLYLERLRLTESDKLQVQI
                     QAYLDNVFDVGTLLEETETKNAVLEHMEELQEQVATLTERLRDTENDSMAKIAELEKQ
                     LSQARKELETLRERFSESTPMGTSRRIPEPEKVPVPTVVRPSALELKVEELEEKGLIR
                     ILRGPGDVVSIEILPGAAATPSGDDAQAPRVSTDSPSTAESIPEAASPPPPPPPPPPP
                     LPNLQSQQEAPPSAPPLAPPLPGCAEPPPAPPLPGDLPPPPPPPPLGTDGPVPPPPPP
                     PPGGPPDILGGQGPDIGPGVKAKKPIQTKFRMPLLNWVALKPSQITGTVFTELNDEKV
                     LQELDMNDFEEHFKTKSQGPCLDISALKGKASQKAPTKTILIEANRAKNLAITLRKGN
                     LGADRICQAIETYDLQTLSLDFLELLTRFLPTDYERSLIARFEKEQRPMEELSEEDRF
                     MLRFSRIQRLPERMNTLTFLGNFPDTAQLLMPQLNAIIAASMSIKSSDKLRQILEIVL
                     AFGNYMNSSKRGAAYGFRLQSLDALLEMKSTDRKQTLLHYLVKVIAEKYPQLTGFHSD
                     LHFLDKAGSVSLDSVLGDVRSLQRGLELTQREFVRQDDCLVLKEFLRANSPTMDKLLA
                     DSKTAQEAYESVVEYFGENPKTTSPSMFFSLFSRFTKAYKKAEQEVEQWKKEAAADTS
                     GREEPPTPKSPPKARRQQMDLISELKRKQQKEPLIYESDRDGAIEDIITDLRNQPYIR
                     ADTGRRSARRRPPGPPLPVTTDLAL"
     exon            355..438
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            439..552
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            553..626
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            627..710
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            711..836
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            837..893
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            894..1002
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1079
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1173
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1248
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1359
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1509
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            1510..1611
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            1612..1864
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            1865..2152
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2277
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            2278..2480
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            2481..2715
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            2716..2784
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            2785..2865
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            2866..2995
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            2996..3159
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            3160..3261
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            3262..3342
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            3343..3463
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     exon            3464..3880
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3862..3867
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /note="hexamer: AATAAA"
     polyA_site      3880
                     /gene="Fmnl1"
                     /gene_synonym="8030453N10Rik; Fmnl; Fnrl; Frls"
                     /note="major polyA site"
ORIGIN      
        1 acttcctccc cgagcctcgg tcgccgcccg ctccctcgga cggggccgcc gcgatgggac
       61 gccgcgcccc ggcccctgca cgccgctgag ccgagagcca cctagccaag cctcgccaca
      121 cagcgctgcc cttcgcttcc aggctcccgg agcctagtcc gcgaccccca gggaaaactg
      181 gatctccgag gctggaggcg cctggccggc tgggtgggga ccaccatggg caacgcggct
      241 ggcagcgccg aacagcccgc gggccccacc gcgtcgcccc cgaagcagcc agccgtcccc
      301 aagcaaccaa tgccggcggc cggggagctg gaggagaggt tcacgcgggt cttgaactgt
      361 atgaacttgc ctccagacaa ggtccagctg ctgagccagt atgacaatga gaagaaatgg
      421 gagctcatct gtgaccagga gcggtttcaa gtcaagaatc cccctgcagc ctacatccag
      481 aagctgaaga gctacctgga taccggtggg gtcagccgaa aggtagcatc tgattggatg
      541 tccaacctgg ggtttaagag gcgagttcag gagtctacac aggtgctgcg ggaactggag
      601 acttccctga ggacaaacca cattgggtgg gtgcaggagt tcctcaatga agagaaccgg
      661 ggtctggatg tcctgcttga gtacctggcc tttgcccagt gctctgttgc atatgacatg
      721 gaaagcacag acagtgtggc ctctggtgca gagaagagca agcccctgga ccagtctgtg
      781 gaagacctca gcaaggcacc tccctcatct gtgcccaaaa gtcgcctgac catcaagtgc
      841 cccccttctc cccggtacgt acataggcac ctgggggcag gtgcatgctg ggggctgacc
      901 cctgcccaca gcaggaaggc gctgaggaat tcccgaatcg tcagccagaa ggatgatgtc
      961 cacgtgtgca tcatgtgtct aagagccatc atgaactacc agtccggctt cagccttgtt
     1021 atgaaccacc cagcctgtgt caatgagatc gctctaagcc ttaacaacaa gagccccaga
     1081 accaaggctc tggtactgga gctgctggca gctgtatgtc tcgtgcgggg aggacacgac
     1141 atcatccttg cggcctttga caacttcaag gaggtttgcg gggagcagca ccgatttgaa
     1201 aagctaatgg agtatttccg gcacgaagac agcaacattg acttcatggt ggcttgcatg
     1261 caattcatca acattgtggt gcactctgtg gagaatatga acttccgtgt cttcctgcaa
     1321 tatgagttca ctcacctggg tctggacctg tacttggaga ggctccggct cactgagagt
     1381 gacaagctgc aggtgcagat ccaggcgtat ctggacaacg tttttgatgt ggggacattg
     1441 ttggaggaga cagagacgaa gaatgcagtg ttggagcaca tggaggaact gcaggagcag
     1501 gtggcaacgc tgacagagcg gcttcgggac acagagaacg actccatggc caagatcgct
     1561 gaactggaga agcagctaag ccaggctcgc aaggaattgg agaccctgag ggagcgcttc
     1621 agcgagtcga cccccatggg cacttccaga cgaatccctg aacctgagaa agtgcctgtc
     1681 cctaccgtgg tgcgaccctc ggctctagaa ctgaaggtgg aggagcttga ggaaaagggg
     1741 ttaatccgca tcctgcgggg gccgggggac gtcgtttcca tcgagatcct tccaggcgct
     1801 gcggcgacac caagcggtga cgacgcacaa gctccgaggg tgtccactga ttcccctagc
     1861 acagcagagt caattcccga agcagcatca ccaccgcctc cgccacctcc accaccacct
     1921 ccactaccta acctccagtc ccagcaggaa gcccctccct ccgcaccccc gctggccccg
     1981 cccctcccag gctgcgcaga gccgccacct gcaccaccgt tgcctggaga cctgcctccc
     2041 ccacctccgc cacccccact tggtactgat ggaccagtgc caccgccacc gccaccgcct
     2101 ccgggagggc cccctgatat ccttggagga caaggcccag atatcggccc aggggtgaaa
     2161 gccaagaaac ccatccagac caagttccgg atgccactgc ttaactgggt ggcgctgaaa
     2221 cccagccaga tcacaggcac tgtcttcacg gagctcaatg acgagaaggt gctccaggag
     2281 ctagacatga atgactttga ggaacacttt aagaccaaat cccaaggtcc ctgcctggat
     2341 atcagcgctc tgaaggggaa ggcctcccag aaggccccca ctaagacaat actcattgaa
     2401 gccaaccggg ccaagaattt ggctatcacc ctgcgcaagg gcaacctagg agcagacaga
     2461 atctgccagg ccatcgagac gtatgaccta cagactctca gcttggattt cttggagctg
     2521 ttgacccgct tcctgcccac ggactacgag cgtagcctca tagcccgatt cgagaaggaa
     2581 cagcggccga tggaagagct gtcagaggag gaccgcttca tgcttcgctt cagtcgcatc
     2641 cagagacttc cggagcgcat gaacacactc accttcctgg gcaactttcc agacacggcc
     2701 cagctgctca tgccgcaact gaacgccatc attgcagcct ctatgtctat caagtcctct
     2761 gacaagcttc gccagatcct agagattgtc ctggcttttg gcaactacat gaacagtagc
     2821 aagcgtgggg cagcctatgg tttccgactt cagagtctgg atgcgttgtt ggagatgaag
     2881 tcgaccgacc ggaaacagac gttgttgcac tacttggtga aggtcattgc tgagaagtac
     2941 ccacagctca cgggttttca tagtgatctg cactttttgg acaaggcagg ctcagtgtcc
     3001 ctggacagcg tactgggaga cgtgcgctcc ttgcagcgag gtctggagct gacgcagaga
     3061 gagtttgtgc ggcaagatga ctgcctggtg ctcaaggagt tcctaagggc caactctccc
     3121 accatggaca agttgctggc agacagcaag acagctcagg aggcctatga gtctgtggtg
     3181 gagtactttg gagaaaatcc caagaccaca tccccctcca tgttcttttc cctcttcagt
     3241 cgcttcacca aggcctacaa gaaagcggaa caggaggtcg aacagtggaa gaaggaagca
     3301 gctgctgata cctcaggcag ggaagagcct ccaacaccca agtctccacc caaggcccgg
     3361 cgacaacaga tggacctcat ctctgagcta aaacggaagc agcagaaaga gccactcatc
     3421 tacgagagtg accgagatgg ggccatcgag gacatcatca cagatcttcg gaaccagccc
     3481 tacatccgtg cagacacagg acgtcgaagt gctcgccggc gcccccctgg acccccactg
     3541 ccggtcacta ctgacctcgc gctgtagtca ctgtttctgc tggtggattc taaaaggctg
     3601 taggcaggct ggagctcaaa gaaggtggtc cttagctcgg ctgggtcggg cgtcctctcc
     3661 tcctgccgac tactagcctt gaagactcct ccactccact ccacccccac cccccacccc
     3721 cccaaaaact ggtcgtagca ccctctccct ccaatagcca tactcagcct gagcgggagc
     3781 ctggcctgta acttataaag tgcacctcgc cgccgcccta gctcggaaga ccctctatgg
     3841 accttatttt tatacaagat taataaagat gtttgcaaaa
//
