LOCUS       NM_146018               3460 bp    mRNA    linear   ROD 25-FEB-2025
DEFINITION  Mus musculus folliculin (Flcn), transcript variant 2, mRNA.
ACCESSION   NM_146018
VERSION     NM_146018.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Raynor,J.L., Collins,N., Shi,H., Guy,C., Saravia,J., Ah Lim,S.,
            Chapman,N.M., Zhou,P., Wang,Y., Sun,Y., Risch,I., Hu,H., Kc,A.,
            Sun,R., Shrestha,S., Huang,H., Connelly,J.P., Pruett-Miller,S.M.,
            Reina-Campos,M., Goldrath,A.W., Belkaid,Y. and Chi,H.
  TITLE     CRISPR screens unveil nutrient-dependent lysosomal and
            mitochondrial nodes impacting intestinal tissue-resident memory
            CD8+ T cell formation
  JOURNAL   Immunity 57 (11), 2597-2614 (2024)
   PUBMED   39406246
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Guo,C., You,Z., Shi,H., Sun,Y., Du,X., Palacios,G., Guy,C.,
            Yuan,S., Chapman,N.M., Lim,S.A., Sun,X., Saravia,J., Rankin,S.,
            Dhungana,Y. and Chi,H.
  TITLE     SLC38A2 and glutamine signalling in cDC1s dictate anti-tumour
            immunity
  JOURNAL   Nature 620 (7972), 200-208 (2023)
   PUBMED   37407815
REFERENCE   3  (bases 1 to 3460)
  AUTHORS   Zhang,X., Li,S., Malik,I., Do,M.H., Ji,L., Chou,C., Shi,W.,
            Capistrano,K.J., Zhang,J., Hsu,T.W., Nixon,B.G., Xu,K., Wang,X.,
            Ballabio,A., Schmidt,L.S., Linehan,W.M. and Li,M.O.
  TITLE     Reprogramming tumour-associated macrophages to outcompete cancer
            cells
  JOURNAL   Nature 619 (7970), 616-623 (2023)
   PUBMED   37380769
REFERENCE   4  (bases 1 to 3460)
  AUTHORS   Di Malta,C., Zampelli,A., Granieri,L., Vilardo,C., De Cegli,R.,
            Cinque,L., Nusco,E., Pece,S., Tosoni,D., Sanguedolce,F.,
            Sorrentino,N.C., Merino,M.J., Nielsen,D., Srinivasan,R., Ball,M.W.,
            Ricketts,C.J., Vocke,C.D., Lang,M., Karim,B., Lanfrancone,L.,
            Schmidt,L.S., Linehan,W.M. and Ballabio,A.
  TITLE     TFEB and TFE3 drive kidney cystogenesis and tumorigenesis
  JOURNAL   EMBO Mol Med 15 (5), e16877 (2023)
   PUBMED   36987696
REFERENCE   5  (bases 1 to 3460)
  AUTHORS   Shillingford,J.M. and Shayman,J.A.
  TITLE     Functional TFEB activation characterizes multiple models of renal
            cystic disease and loss of polycystin-1
  JOURNAL   Am J Physiol Renal Physiol 324 (4), F404-F422 (2023)
   PUBMED   36794754
REFERENCE   6  (bases 1 to 3460)
  AUTHORS   Hudon,V., Sabourin,S., Dydensborg,A.B., Kottis,V., Ghazi,A.,
            Paquet,M., Crosby,K., Pomerleau,V., Uetani,N. and Pause,A.
  TITLE     Renal tumour suppressor function of the Birt-Hogg-Dube syndrome
            gene product folliculin
  JOURNAL   J Med Genet 47 (3), 182-189 (2010)
   PUBMED   19843504
  REMARK    GeneRIF: FLCN is shown as a general tumour suppressor in the
            kidney.
REFERENCE   7  (bases 1 to 3460)
  AUTHORS   Hartman,T.R., Nicolas,E., Klein-Szanto,A., Al-Saleem,T., Cash,T.P.,
            Simon,M.C. and Henske,E.P.
  TITLE     The role of the Birt-Hogg-Dube protein in mTOR activation and renal
            tumorigenesis
  JOURNAL   Oncogene 28 (13), 1594-1604 (2009)
   PUBMED   19234517
REFERENCE   8  (bases 1 to 3460)
  AUTHORS   Baba,M., Furihata,M., Hong,S.B., Tessarollo,L., Haines,D.C.,
            Southon,E., Patel,V., Igarashi,P., Alvord,W.G., Leighty,R., Yao,M.,
            Bernardo,M., Ileva,L., Choyke,P., Warren,M.B., Zbar,B.,
            Linehan,W.M. and Schmidt,L.S.
  TITLE     Kidney-targeted Birt-Hogg-Dube gene inactivation in a mouse model:
            Erk1/2 and Akt-mTOR activation, cell hyperproliferation, and
            polycystic kidneys
  JOURNAL   J Natl Cancer Inst 100 (2), 140-154 (2008)
   PUBMED   18182616
  REMARK    GeneRIF: Homozygous loss of BHD may initiate renal tumorigenesis in
            the mouse. The conditional BHD knockout mouse may be a useful
            research model for dissecting multistep kidney carcinogenesis, and
            rapamycin may be a potential treatment for Birt-Hogg-Dube syndrome
REFERENCE   9  (bases 1 to 3460)
  AUTHORS   Chen,J., Futami,K., Petillo,D., Peng,J., Wang,P., Knol,J., Li,Y.,
            Khoo,S.K., Huang,D., Qian,C.N., Zhao,P., Dykema,K., Zhang,R.,
            Cao,B., Yang,X.J., Furge,K., Williams,B.O. and Teh,B.T.
  TITLE     Deficiency of FLCN in mouse kidney led to development of polycystic
            kidneys and renal neoplasia
  JOURNAL   PLoS One 3 (10), e3581 (2008)
   PUBMED   18974783
  REMARK    GeneRIF: Deficiency of FLCN in mouse kidney led to development of
            polycystic kidneys and renal neoplasia.
            Erratum:[PLoS ONE. 2008;3(11). doi:
            10.1371/annotation/0f06471c-1993-4144-b785-d58924ac0b5c]
REFERENCE   10 (bases 1 to 3460)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ059253.1, AK080933.1 and AK039106.1.
            
            On Sep 19, 2012 this sequence version replaced NM_146018.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK039106.1, AK154040.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-216               CJ059253.1         2-217
            217-3115            AK080933.1         2-2900
            3116-3460           AK039106.1         2904-3248
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 37.79 cM"
     gene            1..3460
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
                     /note="folliculin"
                     /db_xref="GeneID:216805"
                     /db_xref="MGI:MGI:2442184"
     exon            1..380
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
                     /inference="alignment:Splign:2.1.0"
     exon            381..496
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
                     /inference="alignment:Splign:2.1.0"
     exon            497..767
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
                     /inference="alignment:Splign:2.1.0"
     CDS             519..2258
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
                     /EC_number="2.7.1.21"
                     /note="birt-Hogg-Dube syndrome protein homolog"
                     /codon_start=1
                     /product="folliculin"
                     /protein_id="NP_666130.1"
                     /db_xref="CCDS:CCDS24777.1"
                     /db_xref="GeneID:216805"
                     /db_xref="MGI:MGI:2442184"
                     /translation="MNAIVALCHFCELHGPRTLFCTEVLHAPLPQGAGSGDSPDQVEQ
                     AEEEEGGIQMSSRVRAHSPAEGASSESSSPGPKKSDMCEGCRSLAVGHPGYISHDKET
                     SIKYVSHQHPNHPQLFSIVRQACVRSLSCEVCPGREGPIFFGDEQHGFVFSHTFFIKD
                     SLARGFQRWYSIIAIMMDRIYLINSWPFLLGRIRGIISELQAKAFKVFEAEQFGCPQR
                     AQRMNTAFTPFLHQRNGNAARSLTSLTSDDNLWACLHTSFAWLLKACGSRLTEKLLEG
                     APTEDTLVQMEKLADLEEESESWDNSEAEEEEKAPVTPEGAEGRELTSCPTESSFLSA
                     CGSWQPPKLTGFKSLRHMRQVLGAPSFRMLAWHVLMGNQVIWKSRDVNLVHSAFEVLR
                     TMLPVGCVRIIPYSSQYEEAYRCNFLGLSPPVPIPAHVLASEFVVVVEVHTATRSNLH
                     PAGCEDDQSLSKYEFVVTSGSPVAADRVGPTILNKIEAALTNQNLSVDVVDQCLICLK
                     EEWMNKVKVLFKFTKVDSRPKEDTQKLLSVLGASEEDNVKLLKFWMTGLSKTYKSHLM
                     STVRSPTATESRS"
     exon            768..914
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1136
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
                     /inference="alignment:Splign:2.1.0"
     exon            1137..1297
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1389
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1580
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
                     /inference="alignment:Splign:2.1.0"
     exon            1581..1694
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1818
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
                     /inference="alignment:Splign:2.1.0"
     exon            1819..1950
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
                     /inference="alignment:Splign:2.1.0"
     exon            1951..2056
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
                     /inference="alignment:Splign:2.1.0"
     exon            2057..3460
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3097..3102
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
                     /note="hexamer: AATAAA"
     polyA_site      3118
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
                     /note="major polyA site"
     regulatory      3393..3398
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
                     /note="hexamer: ATTAAA"
     polyA_site      3415
                     /gene="Flcn"
                     /gene_synonym="B430214A04Rik; Bhd; FLCL"
ORIGIN      
        1 gcttttccgc ggggcgactt gccgcgccga ggggcgcgcg tggccgaggg gcttggattc
       61 cttgcctgga ggagcattac gtgacacagc aaggctccgc acatcctagt cgcctgcggg
      121 ggacgcaacg ctgctcgctc cggctagtca ctggcgttgc ctgccctctg ccggcctgcg
      181 gatggttcgg tccggctggg tcacgcgcta aggctcagtg caagggcctg cggaacgggc
      241 tagcacttgc cgaggggcag aacagtggcg acagccccag gacagttgcg agcgggttcc
      301 ggcccagcat ccgggagacg gcggcaagcg ccccagctgg gttggtgttg ggccataggg
      361 ctgaatggaa agcgcggatg acaacctcaa gtctttgaat tcgaatagtg cagcttgctt
      421 acctgactct tccggcgggc ctcgtacatg ttctgctcta ggcgggatgg tgcagttgtg
      481 atgtgctaag cataaggcct cggccattct ccagcaccat gaacgccata gtcgccctct
      541 gccacttctg cgagctccat ggcccccgca cgctcttctg cacggaagtt ctacacgctc
      601 ccctgcccca gggggccgga agtggggaca gtcctgacca ggttgagcag gctgaggagg
      661 aggagggtgg cattcagatg agcagccggg tccgtgccca cagcccagcc gagggtgcca
      721 gcagtgagtc cagcagcccg gggcccaaga agtcggacat gtgtgagggc tgccggtcac
      781 ttgccgtagg gcacccaggc tatatcagtc atgataaaga gacctctatt aagtacgtca
      841 gtcaccagca ccccaaccac ccgcagctct tcagcatcgt ccgccaggcc tgtgtccgga
      901 gcctgagctg tgaggtatgc cctggtcgtg aaggccccat cttctttggt gatgagcagc
      961 acggctttgt gttcagccac accttcttca tcaaagacag cctggccaga ggcttccagc
     1021 gctggtacag catcatcgcc atcatgatgg atcggatcta cctcatcaac tcctggccct
     1081 tcctgctggg gaggatccgc ggcatcatca gtgagctcca ggccaaggcc ttcaaggtgt
     1141 ttgaggcaga gcagtttgga tgtccacagc gtgcccagag gatgaacact gccttcacgc
     1201 ccttcctgca ccagaggaac ggcaacgctg cccgctctct gacctccttg accagtgatg
     1261 acaacttgtg ggcgtgtctg cacacttcct ttgcctggct cctgaaggca tgcggtagca
     1321 ggctgacaga aaagctctta gagggcgctc ccacagagga caccctggtc cagatggaga
     1381 agcttgctga cttggaggaa gaatcagaaa gttgggacaa ttctgaggct gaggaggagg
     1441 agaaagctcc tgttacacca gagggtgctg aagggcgaga gctgaccagt tgcccaacag
     1501 agtcatcctt tctctcagcc tgtgggagct ggcagccccc aaagcttacc ggcttcaagt
     1561 ctcttcgaca catgagacag gtcttgggtg ctccatcctt ccgtatgttg gcttggcatg
     1621 tcctcatggg gaatcaggtg atctggaaaa gcagagatgt gaacctggtc cattcagcgt
     1681 ttgaagtcct ccggaccatg ctgcctgtgg gctgtgtccg catcatccct tacagcagcc
     1741 agtatgagga ggcctatcgc tgcaacttcc tggggctcag ccctcccgtg cctatccctg
     1801 cccatgttct ggcctcagag ttcgtagttg tcgtggaggt ccacacggcc actcgctcaa
     1861 acctccaccc tgctgggtgc gaggatgacc agtccctcag caagtatgag tttgtggtga
     1921 ccagtggtag ccctgtggct gcagacagag ttgggcccac tatcctgaat aagattgaag
     1981 cagctctgac caaccagaac ctgtctgtgg atgtggtgga ccaatgtctc atctgcctca
     2041 aggaggaatg gatgaacaaa gtgaaagtcc tgtttaaatt caccaaggta gacagtcgcc
     2101 ccaaggagga cacacagaag ctcctaagcg tcctaggcgc atcagaggag gacaacgtca
     2161 aactgctgaa gttctggatg acgggactga gcaaaaccta caagtcccat ctcatgtcca
     2221 ccgtccgaag ccccacagct acagagtcac ggagctgact ccgagaactc cttctggaag
     2281 gtggtgtaca gaccagctct gtgggaaaaa ctgcccttgg gtttctgact tctggggtga
     2341 ggccctgttt atggcctagg gttcaccctc cttgtaagac tctatcagcc catgttgaaa
     2401 tgtaggggac acagagacag tggtcccttt gcatcaaact gcgctgtcag gacctggcga
     2461 gatgtgttct gtgccccgtg aggacaaagg ctgggaactg agaggagtcc atcagtctgc
     2521 ctctgaggtt ttgttgttgg agcgtgtcct catggggtta ggacaacgcc ttcccaccat
     2581 ggaagacagc aagcatcgtg gatcatttgt ctttcggttg gtgacttctc aggtcacgtg
     2641 cacagccgtg gcctctgcac atcttgccgg cctgtggata catcaaaacc atggctgttt
     2701 tgtttgcttt tgtttttttt taattttggt ttttgagtca gggtcttatt ctgtatccta
     2761 tcccagttta gaactcatgg tgtggcctct gctacttcca gacttgtagc aatcctcctg
     2821 cctcagcctc ccaagtgctg ggattaccga actgagccat cagaacatta cattgctttc
     2881 ttctctgttg ctgccgtagc gctaaccagc cactgtagac tatgacggtg ttacgacaga
     2941 tcgcctctca ccattgcagt ttaataagga cgtagcttgg aaagatttcc tgaaagcttt
     3001 cccaatttgt ttttataata ttcttggtgt ttgtttatat ttaacatgaa gactgtgtat
     3061 ctcagtctct gtacagttgc aagaatgttg aaacacaata aatctgtatt tttgtaaact
     3121 gccgactaca tgtttttatg ttaaatactg tggtggtttg tatgtgctct gcccagggag
     3181 tggcactatt aggaggtgca gccctgttgg agtaggtgtg tcactgtggg tgtggcttta
     3241 agaccctcct cttagctgcc tggaagtcag tatcctacta gcagccttca gatgaagatg
     3301 gagaactctt agctcctcct gctccatgcc tgcctggaag ctgccatgtt cccaccttga
     3361 tgataatgga ctgaaactat aagacagccc caattaaatg ttgtccttat gagaattgcc
     3421 ttggtcatgg tgtctgttca tatcaataaa actgctaacc
//
